FvH4_2g29210

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001445
Physical Location & Seq
Forward (+)
22699277 .. 22704315
5039 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29210.t4

Sequence Viewer

Length: 1281 bp
ATGGGCGAAACCAGGGAGGATGTCTACGACGAGGAGCTCGTCGATTACGAGGAGGACGACGAGAAGGCCCCTGACTCCGCGGCCAAAGTCAATGGCGAATCCGCCAAGAAGGGATATGTTGGAATTCACAGCTCAGGATTCAGAGATTTCCTACTAAAGCCGGAGCTTCTACGTGCTATCGTGGACTCGGGATTTGAGCATCCTTCTGAAGTACAACACGAATGCATCCCACAAGCTATTCTGGGAATGGATGTTATCTGCCAAGCTAAATCTGGGATGGGGAAAACGGCTGTGTTTGTCCTATCTACTTTGCAGCAGATTGACCCTGTGGCCGGCCAAGTTTCTGCACTTATTCTTTGCCATACTAGGGAATTAGCTTACCAGATTTGCCATGAGTTTGAGAGATTCAGTACCTACTTGCCAGATCTCAAGGTTGCTGTCTTCTATGGTGGTGTCACTATCAAAAATCACAAGGACTTGCTGAAAAACGAGTGCCCCCACATTGTTGTTGGGACTCCTGGAAGAATACTAGCACTCGCAAGAGATAAAGACCTCTCATTAAAGAATGTGAGGCATTTTATCCTCGACGAGTGTGACAAGATGCTTGAGTCACTTGACATGAGGAGAGATGTGCAAGAGATATTCAAGATGACGCCCCATGATAAGCAAGTAATGATGTTCTCTGCAACACTCAGCAAAGACATCCGACCGGTTTGCAAGAAATTTATGCAAGATCCTATGGAAATATATGTTGATGACGAAGCCAAGTTGACTCTCCATGGTCTAGTACAGCACTACATCAAGTTAACCGAGGCAGAGAAAAACCGCAAGCTGAATGATCTCCTCGATGCACTGGACTTCAACCAGGTCGTCATATTCGTTAAAAGCGTGAGCAGAGCGGCAGAGTTGAACAAGTTACTCTCAGAGTGTAATTTTCCCTCGATATGTATCCATTCTGGAATGAACCAAGAAGAAAGGTTGAAGCGCTACAAAGGATTCAAGGAGGGTCAGAACAGGATTCTTGTCGCCACTGATTTGGTTGGTAGAGGAATTGATATTGAACGTGTCAATATTGTTATCAATTATGACATGCCAGACTCTGCTGACACCTACTTGCACAGGGTCGGCAGAGCTGGTAGGTTCGGCACCAAAGGGCTGGCGATTACATTTGTATCATCTGCTTCGGACTCTGATGTCCTTAATGATGTTCAGGAGAGGTTTGAGGTTGACATAAAGGAGCTTCCTGAACAAATTGACACTGCCACATACATGCCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

427

Amino Acids

48.11

Weight (kDa)

5.29

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 70 - 235 5.6e-41 DEAD/DEAH box helicase
ResIII PF04851 85 - 232 2.1e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 274 - 382 6.8e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1193
AccB1I GGYRCC 1 cut(s) 1145
AccBSI CCGCTC 1 cut(s) 899
AccI GTMKAC 1 cut(s) 24
AccII CGCG 1 cut(s) 80
AciI CCGC 5 cut(s) 78, 80, 102, 826, 899
AclWI GGATC 1 cut(s) 728
AcoI YGGCCR 3 cut(s) 81, 330, 334
AcsI RAATTY 2 cut(s) 123, 722
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 653
AfaI GTAC 3 cut(s) 213, 412, 789
AfeI AGCGCT 1 cut(s) 986
AfiI CCNNNNNNNGG 2 cut(s) 332, 367
AflIII ACRYGT 1 cut(s) 1063
AgeI ACCGGT 1 cut(s) 709
AgsI TTSAA 6 cut(s) 646, 862, 910, 982, 1000, 1061
AjnI CCWGG 3 cut(s) 11, 517, 864
AluBI AGCT 9 cut(s) 37, 132, 166, 236, 266, 377, 832, 1133, 1240
AluI AGCT 9 cut(s) 37, 132, 166, 236, 266, 377, 832, 1133, 1240
Alw21I GWGCWC 1 cut(s) 39
AlwI GGATC 1 cut(s) 728
AlwNI CAGNNNCTG 1 cut(s) 1100
Ama87I CYCGRG 1 cut(s) 187
Aor51HI AGCGCT 1 cut(s) 986
AoxI GGCC 4 cut(s) 66, 81, 330, 334
ApeKI GCWGC 1 cut(s) 313
ApoI RAATTY 2 cut(s) 123, 722
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
AsiGI ACCGGT 1 cut(s) 709
AspLEI GCGC 1 cut(s) 987
AspS9I GGNCC 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 187
BaeGI GKGCMC 1 cut(s) 497
BaeI ACNNNNGTAYC 2 cut(s) 1155, 1188
BanI GGYRCC 1 cut(s) 1145
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 433
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 325
BccI CCATC 1 cut(s) 271
BceAI ACGGC 1 cut(s) 303
BcgI CGANNNNNNTGC 2 cut(s) 696, 730
BciT130I CCWGG 3 cut(s) 13, 519, 866
BciVI GTATCC 1 cut(s) 959
BfaI CTAG 3 cut(s) 366, 530, 785
BfoI RGCGCY 1 cut(s) 988
BfuI GTATCC 1 cut(s) 959
BglII AGATCT 1 cut(s) 424
BisI GCNGC 3 cut(s) 81, 314, 900
BlsI GCNGC 3 cut(s) 82, 315, 901
Bme1390I CCNGG 3 cut(s) 13, 519, 866
BmeT110I CYCGRG 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 2 cut(s) 69, 1147
BmrFI CCNGG 3 cut(s) 13, 519, 866
BmsI GCATC 4 cut(s) 208, 234, 591, 838
BpiI GAAGAC 1 cut(s) 433
Bpu10I CCTNAGC 1 cut(s) 133
BpuEI CTTGAG 2 cut(s) 413, 626
BsaAI YACGTR 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 947
BsaHI GRCGYC 1 cut(s) 653
BsaJI CCNNGG 4 cut(s) 12, 78, 778, 810
BsaWI WCCGGW 1 cut(s) 709
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 367
Bse118I RCCGGY 2 cut(s) 332, 709
Bse1I ACTGG 1 cut(s) 858
Bse8I GATNNNNATC 1 cut(s) 947
BseBI CCWGG 3 cut(s) 13, 519, 866
BseDI CCNNGG 4 cut(s) 12, 78, 778, 810
BseGI GGATG 6 cut(s) 25, 199, 225, 256, 282, 702
BseJI GATNNNNATC 1 cut(s) 947
BseLI CCNNNNNNNGG 2 cut(s) 332, 367
BseMII CTCAG 3 cut(s) 147, 706, 936
BseNI ACTGG 1 cut(s) 858
BseRI GAGGAG 4 cut(s) 47, 65, 637, 833
BseSI GKGCMC 1 cut(s) 497
BseXI GCAGC 1 cut(s) 325
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 1 cut(s) 80
Bsh1285I CGRYCG 1 cut(s) 710
BshFI GGCC 4 cut(s) 68, 83, 332, 336
BshNI GGYRCC 1 cut(s) 1145
BshTI ACCGGT 1 cut(s) 709
BsiEI CGRYCG 1 cut(s) 710
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 187
BsiSI CCGG 3 cut(s) 161, 333, 710
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 2 cut(s) 332, 367
BsmFI GGGAC 1 cut(s) 526
BsmI GAATGC 1 cut(s) 227
BsnI GGCC 4 cut(s) 68, 83, 332, 336
BsoBI CYCGRG 1 cut(s) 187
Bsp1286I GDGCHC 2 cut(s) 39, 497
Bsp143I GATC 3 cut(s) 424, 733, 838
Bsp19I CCATGG 1 cut(s) 778
BspACI CCGC 5 cut(s) 78, 80, 102, 826, 899
BspANI GGCC 4 cut(s) 68, 83, 332, 336
BspCNI CTCAG 3 cut(s) 146, 705, 935
BspFNI CGCG 1 cut(s) 80
BspLI GGNNCC 2 cut(s) 69, 1147
BspPI GGATC 1 cut(s) 728
BspT107I GGYRCC 1 cut(s) 1145
BsrBI CCGCTC 1 cut(s) 899
BsrFI RCCGGY 2 cut(s) 332, 709
BsrI ACTGG 1 cut(s) 858
BssAI RCCGGY 2 cut(s) 332, 709
BssECI CCNNGG 4 cut(s) 12, 78, 778, 810
BssMI GATC 3 cut(s) 424, 733, 838
BssNI GRCGYC 1 cut(s) 653
BssT1I CCWWGG 1 cut(s) 778
Bst2UI CCWGG 3 cut(s) 13, 519, 866
BstACI GRCGYC 1 cut(s) 653
BstBAI YACGTR 1 cut(s) 173
BstC8I GCNNGC 3 cut(s) 334, 830, 1158
BstDEI CTNAG 3 cut(s) 133, 692, 922
BstDSI CCRYGG 2 cut(s) 78, 778
BstF5I GGATG 6 cut(s) 25, 199, 225, 256, 282, 702
BstFNI CGCG 1 cut(s) 80
BstH2I RGCGCY 1 cut(s) 988
BstHHI GCGC 1 cut(s) 987
BstKTI GATC 3 cut(s) 427, 736, 841
BstMBI GATC 3 cut(s) 424, 733, 838
BstMCI CGRYCG 1 cut(s) 710
BstNI CCWGG 3 cut(s) 13, 519, 866
BstNSI RCATGY 2 cut(s) 1093, 1273
BstSCI CCNGG 3 cut(s) 11, 517, 864
BstSLI GKGCMC 1 cut(s) 497
BstUI CGCG 1 cut(s) 80
BstV1I GCAGC 1 cut(s) 325
BstV2I GAAGAC 1 cut(s) 433
BstX2I RGATCY 2 cut(s) 424, 733
BstXI CCANNNNNNTGG 2 cut(s) 1036, 1156
BstYI RGATCY 2 cut(s) 424, 733
BsuI GTATCC 1 cut(s) 959
BsuRI GGCC 4 cut(s) 68, 83, 332, 336
BtgI CCRYGG 2 cut(s) 78, 778
BtsCI GGATG 6 cut(s) 25, 199, 225, 256, 282, 702
BtsI GCAGTG 1 cut(s) 1257
BtsIMutI CAGTG 3 cut(s) 851, 1029, 1257
Cac8I GCNNGC 3 cut(s) 334, 830, 1158
CaiI CAGNNNCTG 1 cut(s) 1100
CfoI GCGC 1 cut(s) 987
Cfr10I RCCGGY 2 cut(s) 332, 709
Cfr13I GGNCC 1 cut(s) 67
Cfr42I CCGCGG 1 cut(s) 81
CseI GACGC 1 cut(s) 661
CsiI ACCWGGT 1 cut(s) 864
Csp6I GTAC 3 cut(s) 212, 411, 788
CspAI ACCGGT 1 cut(s) 709
CviAII CATG 6 cut(s) 392, 619, 659, 779, 1090, 1270
CviQI GTAC 3 cut(s) 212, 411, 788
DdeI CTNAG 3 cut(s) 133, 692, 922
DpnI GATC 3 cut(s) 426, 735, 840
DpnII GATC 3 cut(s) 424, 733, 838
DrdI GACNNNNNNGTC 1 cut(s) 1193
DseDI GACNNNNNNGTC 1 cut(s) 1193
EaeI YGGCCR 3 cut(s) 81, 330, 334
EciI GGCGGA 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 778
Eco24I GRGCYC 1 cut(s) 39
Eco47III AGCGCT 1 cut(s) 986
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 187
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 123
EcoRII CCWGG 3 cut(s) 11, 517, 864
EcoT14I CCWWGG 1 cut(s) 778
EcoT22I ATGCAT 1 cut(s) 227
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 778
FaeI CATG 6 cut(s) 395, 622, 662, 782, 1093, 1273
FaqI GGGAC 1 cut(s) 526
FatI CATG 6 cut(s) 391, 618, 658, 778, 1089, 1269
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 3 cut(s) 81, 314, 900
FokI GGATG 6 cut(s) 32, 186, 212, 263, 289, 689
FriOI GRGCYC 1 cut(s) 39
FseI GGCCGGCC 1 cut(s) 336
Fsp4HI GCNGC 3 cut(s) 81, 314, 900
FspBI CTAG 3 cut(s) 366, 530, 785
GlaI GCGC 1 cut(s) 986
GluI GCNGC 3 cut(s) 81, 314, 900
HaeII RGCGCY 1 cut(s) 988
HaeIII GGCC 4 cut(s) 68, 83, 332, 336
HapII CCGG 3 cut(s) 161, 333, 710
HgaI GACGC 1 cut(s) 661
HhaI GCGC 1 cut(s) 987
Hin1I GRCGYC 1 cut(s) 653
Hin1II CATG 6 cut(s) 395, 622, 662, 782, 1093, 1273
Hin6I GCGC 1 cut(s) 985
HinP1I GCGC 1 cut(s) 985
HincII GTYRAC 3 cut(s) 771, 807, 1228
HindII GTYRAC 3 cut(s) 771, 807, 1228
HpaI GTTAAC 1 cut(s) 807
HpaII CCGG 3 cut(s) 161, 333, 710
Hpy166II GTNNAC 5 cut(s) 25, 184, 771, 807, 1228
Hpy188I TCNGA 7 cut(s) 143, 208, 707, 925, 1011, 1186, 1192
Hpy188III TCNNGA 6 cut(s) 135, 189, 646, 957, 1211, 1244
Hpy8I GTNNAC 5 cut(s) 25, 184, 771, 807, 1228
Hpy99I CGWCG 4 cut(s) 32, 44, 62, 590
HpyAV CCTTC 3 cut(s) 58, 103, 213
HpyCH4IV ACGT 2 cut(s) 172, 1063
HpyCH4V TGCA 9 cut(s) 225, 313, 347, 634, 686, 717, 730, 851, 1117
HpyF3I CTNAG 3 cut(s) 133, 692, 922
HpySE526I ACGT 2 cut(s) 172, 1063
Hsp92I GRCGYC 1 cut(s) 653
Hsp92II CATG 6 cut(s) 395, 622, 662, 782, 1093, 1273
HspAI GCGC 1 cut(s) 985
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
KspAI GTTAAC 1 cut(s) 807
KspI CCGCGG 1 cut(s) 81
Kzo9I GATC 3 cut(s) 424, 733, 838
LmnI GCTCC 3 cut(s) 34, 163, 1237
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 4 cut(s) 208, 234, 591, 838
MabI ACCWGGT 1 cut(s) 864
MaeI CTAG 3 cut(s) 366, 530, 785
MaeII ACGT 2 cut(s) 172, 1063
MaeIII GTNAC 4 cut(s) 454, 593, 609, 915
MalI GATC 3 cut(s) 426, 735, 840
MbiI CCGCTC 1 cut(s) 899
MboI GATC 3 cut(s) 424, 733, 838
MboII GAAGA 3 cut(s) 433, 534, 983
MflI RGATCY 2 cut(s) 424, 733
MhlI GDGCHC 2 cut(s) 39, 497
MluCI AATT 7 cut(s) 123, 371, 722, 931, 1050, 1081, 1251
MlyI GAGTC 7 cut(s) 68, 179, 508, 617, 766, 1091, 1181
MmeI TCCRAC 2 cut(s) 100, 730
Mph1103I ATGCAT 1 cut(s) 227
MroNI GCCGGC 1 cut(s) 332
MseI TTAA 5 cut(s) 560, 806, 882, 1200, 1279
MslI CAYNNNNRTG 1 cut(s) 1268
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 3 cut(s) 161, 333, 710
MspR9I CCNGG 3 cut(s) 13, 519, 866
Mva1269I GAATGC 1 cut(s) 227
MvaI CCWGG 3 cut(s) 13, 519, 866
MvnI CGCG 1 cut(s) 80
NaeI GCCGGC 1 cut(s) 334
NcoI CCATGG 1 cut(s) 778
NdeII GATC 3 cut(s) 424, 733, 838
NgoMIV GCCGGC 1 cut(s) 332
NlaIII CATG 6 cut(s) 395, 622, 662, 782, 1093, 1273
NlaIV GGNNCC 2 cut(s) 69, 1147
NmuCI GTSAC 3 cut(s) 454, 593, 609
NsiI ATGCAT 1 cut(s) 227
NspI RCATGY 2 cut(s) 1093, 1273
PcsI WCGNNNNNNNCGW 5 cut(s) 36, 45, 54, 876, 885
PctI GAATGC 1 cut(s) 227
PdiI GCCGGC 1 cut(s) 334
PfeI GAWTC 5 cut(s) 98, 138, 405, 996, 1018
PfoI TCCNGGA 1 cut(s) 517
PinAI ACCGGT 1 cut(s) 709
PkrI GCNGC 3 cut(s) 82, 315, 901
PleI GAGTC 7 cut(s) 68, 179, 508, 616, 766, 1091, 1181
PpsI GAGTC 7 cut(s) 68, 179, 508, 616, 766, 1091, 1181
Ppu21I YACGTR 1 cut(s) 173
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 3 cut(s) 11, 517, 864
PspGI CCWGG 3 cut(s) 11, 517, 864
PspN4I GGNNCC 2 cut(s) 69, 1147
PspPI GGNCC 1 cut(s) 67
PstNI CAGNNNCTG 1 cut(s) 1100
PsuI RGATCY 2 cut(s) 424, 733
RigI GGCCGGCC 1 cut(s) 336
RsaI GTAC 3 cut(s) 213, 412, 789
RsaNI GTAC 3 cut(s) 212, 411, 788
RseI CAYNNNNRTG 1 cut(s) 1268
SacI GAGCTC 1 cut(s) 39
SacII CCGCGG 1 cut(s) 81
SaqAI TTAA 5 cut(s) 560, 806, 882, 1200, 1279
SatI GCNGC 3 cut(s) 81, 314, 900
Sau3AI GATC 3 cut(s) 424, 733, 838
Sau96I GGNCC 1 cut(s) 67
SchI GAGTC 7 cut(s) 68, 179, 508, 617, 766, 1091, 1181
ScrFI CCNGG 3 cut(s) 13, 519, 866
SduI GDGCHC 2 cut(s) 39, 497
SexAI ACCWGGT 1 cut(s) 864
SfaNI GCATC 4 cut(s) 208, 234, 591, 838
Sfr303I CCGCGG 1 cut(s) 81
SgrBI CCGCGG 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 1268
SmlI CTYRAG 2 cut(s) 428, 605
SmoI CTYRAG 2 cut(s) 428, 605
Sse9I AATT 7 cut(s) 123, 371, 722, 931, 1050, 1081, 1251
SsiI CCGC 5 cut(s) 78, 80, 102, 826, 899
SspI AATATT 1 cut(s) 1072
SspMI CTAG 3 cut(s) 366, 530, 785
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 3 cut(s) 11, 517, 864
StyI CCWWGG 1 cut(s) 778
TaiI ACGT 2 cut(s) 175, 1066
TaqI TCGA 4 cut(s) 42, 585, 846, 941
TasI AATT 7 cut(s) 123, 371, 722, 931, 1050, 1081, 1251
TatI WGTACW 2 cut(s) 211, 787
TauI GCSGC 2 cut(s) 83, 902
TfiI GAWTC 5 cut(s) 98, 138, 405, 996, 1018
Tru1I TTAA 5 cut(s) 560, 806, 882, 1200, 1279
Tru9I TTAA 5 cut(s) 560, 806, 882, 1200, 1279
TscAI CASTG 3 cut(s) 858, 1036, 1264
TseFI GTSAC 3 cut(s) 454, 593, 609
TseI GCWGC 1 cut(s) 313
Tsp45I GTSAC 3 cut(s) 454, 593, 609
TspDTI ATGAA 1 cut(s) 977
TspRI CASTG 3 cut(s) 858, 1036, 1264
XapI RAATTY 2 cut(s) 123, 722
XceI RCATGY 2 cut(s) 1093, 1273
XcmI CCANNNNNNNNNTGG 2 cut(s) 269, 506
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 3 cut(s) 366, 530, 785
Zsp2I ATGCAT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.