Rorug06G0278300

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001445
Physical Location & Seq
Reverse (-)
42602301 .. 42603453
1153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0278300.1

Sequence Viewer

Length: 651 bp
ATGTACTCAGACTTTGCAGCAGCATTAAACTTCACTTACCAAATTAAAAGCTTAGCTTCCCCAGAATATCCAATAAATGTCCCACCAGACAATGACATTACTACCAGAATGTTTATAACAGCTTCCATGAATTCACTTTACTGTGAAGATTCAGGTTTCTTGAGCTGCACAGAGATACCAATCGCTACAAGCTACAAGGAACAAAGGTACGTGAAGGTGTTGAGTTATAATGAGTCAGTTGAGATTGTGTTCCAAGGGACTGATGTGCAAGGAGGCTCTGTGAATCATCCACTGCATATGCATGGATATAAGTTTTATGTCGTTGGATATGGTTTCGGAAATTATGACAATGAGACCGACTCAAAAGGGTTTAATCTTGTTGATCCTCCTCAAGTCACTACATTTGGAATTCCCAAAAATGGATGGTTGGCGATCAGATTCATAGCGAATAATCCTGGTGTTTGGTTTTGGCATTGTCATATGGAGAGACACCTGACTTTGGGTATGGAAGCTGCATTCATAGTGAAGAATGGGGACACCGCTGAAACTAGCATACTCGAACCCCCAGCTTACATGCCTTCCTGTAATGTTCCATTGGCTTCTCGTTTCAAAAATTCTGATGTGTTTGTTGAAAAGAAAATAGATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

216

Amino Acids

24.31

Weight (kDa)

5.16

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 62 - 178 3.1e-37 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 116, 228
AciI CCGC 1 cut(s) 540
AclWI GGATC 1 cut(s) 377
AcsI RAATTY 3 cut(s) 130, 408, 613
AfaI GTAC 2 cut(s) 5, 209
AfiI CCNNNNNNNGG 2 cut(s) 419, 499
AgsI TTSAA 2 cut(s) 610, 632
AjnI CCWGG 1 cut(s) 454
AluBI AGCT 7 cut(s) 51, 56, 122, 165, 192, 512, 569
AluI AGCT 7 cut(s) 51, 56, 122, 165, 192, 512, 569
Alw26I GTCTC 2 cut(s) 347, 481
AlwI GGATC 1 cut(s) 377
ApeKI GCWGC 4 cut(s) 17, 20, 165, 512
ApoI RAATTY 3 cut(s) 130, 408, 613
BbvI GCAGC 4 cut(s) 29, 32, 152, 499
BccI CCATC 1 cut(s) 417
BciT130I CCWGG 1 cut(s) 456
BcoDI GTCTC 2 cut(s) 347, 481
BfaI CTAG 1 cut(s) 549
BisI GCNGC 4 cut(s) 18, 21, 166, 513
BlpI GCTNAGC 1 cut(s) 52
BlsI GCNGC 4 cut(s) 19, 22, 167, 514
Bme1390I CCNGG 1 cut(s) 456
BmrFI CCNGG 1 cut(s) 456
BplI GAGNNNNNCTC 2 cut(s) 344, 376
Bpu1102I GCTNAGC 1 cut(s) 52
BpuEI CTTGAG 2 cut(s) 181, 375
BsaAI YACGTR 1 cut(s) 211
BsaBI GATNNNNATC 1 cut(s) 179
BsaI GGTCTC 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 253
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 499
Bse8I GATNNNNATC 1 cut(s) 179
BseBI CCWGG 1 cut(s) 456
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 2 cut(s) 286, 428
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 2 cut(s) 419, 499
BseMII CTCAG 1 cut(s) 21
BseRI GAGGAG 1 cut(s) 378
BseXI GCAGC 4 cut(s) 29, 32, 152, 499
BseYI CCCAGC 1 cut(s) 565
BsgI GTGCAG 1 cut(s) 151
BslFI GGGAC 3 cut(s) 65, 271, 548
BslI CCNNNNNNNGG 2 cut(s) 419, 499
BsmAI GTCTC 2 cut(s) 347, 481
BsmFI GGGAC 3 cut(s) 65, 271, 548
BsmI GAATGC 1 cut(s) 515
Bso31I GGTCTC 1 cut(s) 347
Bsp143I GATC 3 cut(s) 382, 432, 643
Bsp1720I GCTNAGC 1 cut(s) 52
BspACI CCGC 1 cut(s) 540
BspCNI CTCAG 1 cut(s) 20
BspPI GGATC 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 3 cut(s) 382, 432, 643
BssT1I CCWWGG 1 cut(s) 253
Bst2UI CCWGG 1 cut(s) 456
Bst4CI ACNGT 1 cut(s) 143
BstBAI YACGTR 1 cut(s) 211
BstDEI CTNAG 2 cut(s) 7, 52
BstF5I GGATG 2 cut(s) 286, 428
BstKTI GATC 3 cut(s) 385, 435, 646
BstMAI GTCTC 2 cut(s) 347, 481
BstMBI GATC 3 cut(s) 382, 432, 643
BstNI CCWGG 1 cut(s) 456
BstNSI RCATGY 1 cut(s) 577
BstSCI CCNGG 1 cut(s) 454
BstV1I GCAGC 4 cut(s) 29, 32, 152, 499
BtsCI GGATG 2 cut(s) 286, 428
BtsI GCAGTG 1 cut(s) 290
BtsIMutI CAGTG 1 cut(s) 290
Csp6I GTAC 2 cut(s) 4, 208
CviAII CATG 3 cut(s) 127, 302, 574
CviJI RGCY 9 cut(s) 51, 56, 122, 165, 192, 276, 512, 569, 599
CviKI_1 RGCY 9 cut(s) 51, 56, 122, 165, 192, 276, 512, 569, 599
CviQI GTAC 2 cut(s) 4, 208
DdeI CTNAG 2 cut(s) 7, 52
DpnI GATC 3 cut(s) 384, 434, 645
DpnII GATC 3 cut(s) 382, 432, 643
Eco130I CCWWGG 1 cut(s) 253
Eco31I GGTCTC 1 cut(s) 347
EcoRI GAATTC 2 cut(s) 130, 408
EcoRII CCWGG 1 cut(s) 454
EcoT14I CCWWGG 1 cut(s) 253
EcoT22I ATGCAT 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 3 cut(s) 130, 305, 577
FalI AAGNNNNNCTT 2 cut(s) 40, 72
FaqI GGGAC 3 cut(s) 65, 271, 548
FatI CATG 3 cut(s) 126, 301, 573
FauNDI CATATG 2 cut(s) 297, 480
Fnu4HI GCNGC 4 cut(s) 18, 21, 166, 513
FokI GGATG 2 cut(s) 273, 435
Fsp4HI GCNGC 4 cut(s) 18, 21, 166, 513
FspBI CTAG 1 cut(s) 549
GluI GCNGC 4 cut(s) 18, 21, 166, 513
GsaI CCCAGC 1 cut(s) 569
Hin1II CATG 3 cut(s) 130, 305, 577
HindIII AAGCTT 1 cut(s) 49
HinfI GANTC 5 cut(s) 149, 233, 283, 359, 438
Hpy188I TCNGA 4 cut(s) 10, 338, 437, 619
Hpy188III TCNNGA 1 cut(s) 160
HpyAV CCTTC 2 cut(s) 208, 588
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 6 cut(s) 17, 168, 268, 295, 301, 515
HpyF3I CTNAG 2 cut(s) 7, 52
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 3 cut(s) 130, 305, 577
Kzo9I GATC 3 cut(s) 382, 432, 643
LpnPI CCDG 9 cut(s) 75, 99, 118, 138, 441, 468, 506, 579, 595
Lsp1109I GCAGC 4 cut(s) 29, 32, 152, 499
MaeI CTAG 1 cut(s) 549
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 394
MalI GATC 3 cut(s) 384, 434, 645
MboI GATC 3 cut(s) 382, 432, 643
MboII GAAGA 2 cut(s) 158, 538
MluCI AATT 5 cut(s) 42, 130, 340, 408, 613
MlyI GAGTC 2 cut(s) 242, 353
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 3 cut(s) 266, 396, 399
Mph1103I ATGCAT 1 cut(s) 303
MseI TTAA 3 cut(s) 26, 45, 372
MslI CAYNNNNRTG 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 542
MspR9I CCNGG 1 cut(s) 456
Mva1269I GAATGC 1 cut(s) 515
MvaI CCWGG 1 cut(s) 456
NdeI CATATG 2 cut(s) 297, 480
NdeII GATC 3 cut(s) 382, 432, 643
NlaIII CATG 3 cut(s) 130, 305, 577
NmuCI GTSAC 1 cut(s) 394
NsiI ATGCAT 1 cut(s) 303
NspI RCATGY 1 cut(s) 577
PctI GAATGC 1 cut(s) 515
PfeI GAWTC 3 cut(s) 149, 283, 438
PkrI GCNGC 4 cut(s) 19, 22, 167, 514
PleI GAGTC 2 cut(s) 241, 353
PpsI GAGTC 2 cut(s) 241, 353
Ppu21I YACGTR 1 cut(s) 211
PsiI TTATAA 2 cut(s) 116, 228
Psp6I CCWGG 1 cut(s) 454
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 1 cut(s) 454
RsaI GTAC 2 cut(s) 5, 209
RsaNI GTAC 2 cut(s) 4, 208
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 3 cut(s) 26, 45, 372
SatI GCNGC 4 cut(s) 18, 21, 166, 513
Sau3AI GATC 3 cut(s) 382, 432, 643
SchI GAGTC 2 cut(s) 242, 353
ScrFI CCNGG 1 cut(s) 456
SmiMI CAYNNNNRTG 1 cut(s) 300
SmlI CTYRAG 2 cut(s) 160, 390
SmoI CTYRAG 2 cut(s) 160, 390
Sse9I AATT 5 cut(s) 42, 130, 340, 408, 613
SsiI CCGC 1 cut(s) 540
SspMI CTAG 1 cut(s) 549
StyD4I CCNGG 1 cut(s) 454
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 1 cut(s) 143
TaiI ACGT 1 cut(s) 213
TaqI TCGA 1 cut(s) 558
TaqII GACCGA 1 cut(s) 371
TasI AATT 5 cut(s) 42, 130, 340, 408, 613
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 3 cut(s) 149, 283, 438
Tru1I TTAA 3 cut(s) 26, 45, 372
Tru9I TTAA 3 cut(s) 26, 45, 372
TscAI CASTG 1 cut(s) 297
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 4 cut(s) 17, 20, 165, 512
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 3 cut(s) 143, 430, 508
TspRI CASTG 1 cut(s) 297
XapI RAATTY 3 cut(s) 130, 408, 613
XceI RCATGY 1 cut(s) 577
XspI CTAG 1 cut(s) 549
Zsp2I ATGCAT 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.