Rroxscaffold_7G00211110

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001445
Physical Location & Seq
Reverse (-)
61555861 .. 61561363
5503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00211110.1

Sequence Viewer

Length: 1023 bp
ATGGAGGTTCATGGGGTTGGACATTCCCCAACATCTAAAACGAACCTAAGTAGTGCTTCGACCCGTCCGCATACATATGTGCTGCAAATTCGGCGCGTTTGGGATCACGGCATTCAACACATTGCTCAACATCCTCCCATTAGCCTCACAGTTCTCGGCTTCGGTTGGCTTGCTCGCCCATGTCACATAAAAAATGTGAGTGACATCGGTGAGTCGGGAGAGCTCGCCTCGGTCCGTGTAGAGTCGGAGAGGTCACACCGGATGTACCGAATCGGGTGGTCGGCGTTCCACCGCGGCCGGGCCGCCGGCCACTCCGTAGACCTTCCACGGCCCGCCGGGAGTGTCGGGCAGTGGAAGAATCTCGGCGAGGCTGTTTCCGACGAGTCCGGTAACCCCAATTTGGTAAAGACTTTCATAAAAGATTGGAGACATTTTGTTTTACTTACCAATGGTTCCTTGGATGCGGTTTTGTTTCGTTCTTCATCGGTACAACATGAATGCATCCCACAAGCTATTCCGGGAATGGATGTTATCTGCCAAGCTAAATGCGGGATGGGGAAAACGGCTGTATTTGTCCTATCTACTTTGCAGCAGATTAACCCTGTCGCAGGCCAAATTTGCCATGAGTTTGAGAGATTCGGTACCTACTTGCCGGATCTTAAGGTTCTTGTCTTCTATGGTGGTGTCACTATCAAAAATCACAAAGATTTACTTAAAAATGAATGCCCCCACATTGTTGTTGGAACTCCTGGAAGAATACTTGCACTTGCAAGAGATAAAGACCTCTCTTTGAAGAATGTGAGGCATTTTATCCTCGATGAATGTGACAAGATGCTTGAGTCACTAGGTATGCACTACATCAAGCTAACCGAGGCAGAGAAAAATCGCAAGCTGAATGATCTCCTTGATGCACCGGACTTCAACCAGGTTGTCATCTTCGTCAAAAGTGTGAGCAGAGCTGCAGAGTTGAACAAGTTACTCTCGAGTGTAATTTTCCCTCTATATGCATCCATTCTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

340

Amino Acids

37.78

Weight (kDa)

8.78

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 162 - 289 9.1e-22 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 641
AccB1I GGYRCC 1 cut(s) 641
AccI GTMKAC 1 cut(s) 318
AccII CGCG 2 cut(s) 96, 294
AciI CCGC 7 cut(s) 68, 292, 294, 303, 333, 464, 549
AclWI GGATC 2 cut(s) 111, 663
AcoI YGGCCR 2 cut(s) 295, 307
AcsI RAATTY 2 cut(s) 87, 615
AfaI GTAC 3 cut(s) 266, 489, 643
AfiI CCNNNNNNNGG 3 cut(s) 298, 400, 608
AflII CTTAAG 1 cut(s) 659
AgsI TTSAA 4 cut(s) 116, 793, 922, 970
AjnI CCWGG 2 cut(s) 748, 924
AluBI AGCT 6 cut(s) 223, 512, 542, 865, 892, 959
AluI AGCT 6 cut(s) 223, 512, 542, 865, 892, 959
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 1 cut(s) 421
AlwI GGATC 2 cut(s) 111, 663
Ama87I CYCGRG 1 cut(s) 982
AoxI GGCC 5 cut(s) 295, 300, 307, 329, 610
ApeKI GCWGC 3 cut(s) 82, 589, 959
ApoI RAATTY 2 cut(s) 87, 615
Asp718I GGTACC 1 cut(s) 641
AspLEI GCGC 1 cut(s) 96
AspS9I GGNCC 3 cut(s) 232, 300, 330
AsuC2I CCSGG 3 cut(s) 299, 337, 519
AsuHPI GGTGA 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 982
AvaII GGWCC 1 cut(s) 232
BanI GGYRCC 1 cut(s) 641
BanII GRGCYC 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 664
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 3 cut(s) 69, 601, 946
BccI CCATC 1 cut(s) 547
BceAI ACGGC 3 cut(s) 124, 344, 579
BciT130I CCWGG 2 cut(s) 750, 926
BcnI CCSGG 3 cut(s) 299, 337, 519
BcoDI GTCTC 1 cut(s) 421
BfaI CTAG 1 cut(s) 845
BfmI CTRYAG 1 cut(s) 960
BfrI CTTAAG 1 cut(s) 659
BisI GCNGC 5 cut(s) 83, 295, 303, 590, 960
BlsI GCNGC 5 cut(s) 84, 296, 304, 591, 961
Bme1390I CCNGG 5 cut(s) 299, 337, 519, 750, 926
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 982
BmgT120I GGNCC 3 cut(s) 232, 300, 330
BmiI GGNNCC 2 cut(s) 454, 643
BmrFI CCNGG 5 cut(s) 299, 337, 519, 750, 926
BmsI GCATC 5 cut(s) 451, 510, 822, 898, 1016
BpiI GAAGAC 1 cut(s) 664
BplI GAGNNNNNCTC 2 cut(s) 212, 244
BpuEI CTTGAG 1 cut(s) 857
BpuMI CCSGG 3 cut(s) 299, 337, 519
BsaJI CCNNGG 5 cut(s) 228, 292, 326, 456, 870
BsaWI WCCGGW 3 cut(s) 258, 386, 913
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 400, 608
Bse118I RCCGGY 1 cut(s) 305
Bse3DI GCAATG 1 cut(s) 120
BseBI CCWGG 2 cut(s) 750, 926
BseDI CCNNGG 5 cut(s) 228, 292, 326, 456, 870
BseGI GGATG 7 cut(s) 130, 267, 466, 501, 532, 558, 1007
BseLI CCNNNNNNNGG 3 cut(s) 298, 400, 608
BseMI GCAATG 1 cut(s) 120
BseX3I CGGCCG 1 cut(s) 295
BseXI GCAGC 3 cut(s) 69, 601, 946
Bsh1236I CGCG 2 cut(s) 96, 294
Bsh1285I CGRYCG 1 cut(s) 298
BshFI GGCC 5 cut(s) 297, 302, 309, 331, 612
BshNI GGYRCC 1 cut(s) 641
BsiEI CGRYCG 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 982
BsiSI CCGG 8 cut(s) 259, 298, 306, 336, 387, 518, 653, 914
BslI CCNNNNNNNGG 3 cut(s) 298, 400, 608
BsmAI GTCTC 1 cut(s) 421
BsmI GAATGC 3 cut(s) 111, 503, 728
BsnI GGCC 5 cut(s) 297, 302, 309, 331, 612
BsoBI CYCGRG 1 cut(s) 982
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 3 cut(s) 103, 655, 898
BspACI CCGC 7 cut(s) 68, 292, 294, 303, 333, 464, 549
BspANI GGCC 5 cut(s) 297, 302, 309, 331, 612
BspFNI CGCG 2 cut(s) 96, 294
BspLI GGNNCC 2 cut(s) 454, 643
BspMAI CTGCAG 1 cut(s) 964
BspPI GGATC 2 cut(s) 111, 663
BspT107I GGYRCC 1 cut(s) 641
BspTI CTTAAG 1 cut(s) 659
BsrDI GCAATG 1 cut(s) 120
BsrFI RCCGGY 1 cut(s) 305
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 5 cut(s) 228, 292, 326, 456, 870
BssMI GATC 3 cut(s) 103, 655, 898
BssT1I CCWWGG 1 cut(s) 456
Bst2UI CCWGG 2 cut(s) 750, 926
Bst4CI ACNGT 1 cut(s) 151
BstAFI CTTAAG 1 cut(s) 659
BstC8I GCNNGC 7 cut(s) 171, 175, 225, 307, 333, 610, 890
BstDEI CTNAG 1 cut(s) 47
BstDSI CCRYGG 2 cut(s) 292, 326
BstEII GGTNACC 1 cut(s) 389
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 7 cut(s) 130, 267, 466, 501, 532, 558, 1007
BstFNI CGCG 2 cut(s) 96, 294
BstHHI GCGC 1 cut(s) 96
BstKTI GATC 3 cut(s) 106, 658, 901
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 3 cut(s) 103, 655, 898
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 2 cut(s) 91, 618
BstNI CCWGG 2 cut(s) 750, 926
BstPI GGTNACC 1 cut(s) 389
BstSCI CCNGG 5 cut(s) 297, 335, 517, 748, 924
BstSFI CTRYAG 1 cut(s) 960
BstUI CGCG 2 cut(s) 96, 294
BstV1I GCAGC 3 cut(s) 69, 601, 946
BstV2I GAAGAC 1 cut(s) 664
BstX2I RGATCY 1 cut(s) 655
BstYI RGATCY 1 cut(s) 655
BstZI CGGCCG 1 cut(s) 295
BsuRI GGCC 5 cut(s) 297, 302, 309, 331, 612
BtgI CCRYGG 2 cut(s) 292, 326
BtsCI GGATG 7 cut(s) 130, 267, 466, 501, 532, 558, 1007
BtsI GCAGTG 1 cut(s) 356
BtsIMutI CAGTG 1 cut(s) 356
Cac8I GCNNGC 7 cut(s) 171, 175, 225, 307, 333, 610, 890
CfoI GCGC 1 cut(s) 96
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 3 cut(s) 232, 300, 330
Cfr42I CCGCGG 1 cut(s) 295
CpoI CGGWCCG 1 cut(s) 232
CsiI ACCWGGT 1 cut(s) 924
Csp6I GTAC 3 cut(s) 265, 488, 642
CspI CGGWCCG 1 cut(s) 232
CviAII CATG 4 cut(s) 11, 180, 494, 623
CviQI GTAC 3 cut(s) 265, 488, 642
DdeI CTNAG 1 cut(s) 47
DpnI GATC 3 cut(s) 105, 657, 900
DpnII GATC 3 cut(s) 103, 655, 898
EaeI YGGCCR 2 cut(s) 295, 307
EagI CGGCCG 1 cut(s) 295
Ecl136II GAGCTC 1 cut(s) 223
EclXI CGGCCG 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 456
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 232
Eco52I CGGCCG 1 cut(s) 295
Eco53kI GAGCTC 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 982
Eco91I GGTNACC 1 cut(s) 389
EcoICRI GAGCTC 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoO65I GGTNACC 1 cut(s) 389
EcoRII CCWGG 2 cut(s) 748, 924
EcoT14I CCWWGG 1 cut(s) 456
EcoT22I ATGCAT 2 cut(s) 503, 1009
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 456
FaeI CATG 4 cut(s) 14, 183, 497, 626
FalI AAGNNNNNCTT 4 cut(s) 40, 72, 696, 728
FatI CATG 4 cut(s) 10, 179, 493, 622
FauI CCCGC 2 cut(s) 340, 542
FauNDI CATATG 1 cut(s) 76
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 5 cut(s) 83, 295, 303, 590, 960
FokI GGATG 7 cut(s) 117, 274, 473, 488, 539, 565, 994
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 5 cut(s) 83, 295, 303, 590, 960
FspBI CTAG 1 cut(s) 845
GlaI GCGC 1 cut(s) 95
GluI GCNGC 5 cut(s) 83, 295, 303, 590, 960
HaeIII GGCC 5 cut(s) 297, 302, 309, 331, 612
HapII CCGG 8 cut(s) 259, 298, 306, 336, 387, 518, 653, 914
HhaI GCGC 1 cut(s) 96
Hin1II CATG 4 cut(s) 14, 183, 497, 626
Hin6I GCGC 1 cut(s) 94
HinP1I GCGC 1 cut(s) 94
HinfI GANTC 7 cut(s) 212, 242, 270, 358, 383, 636, 839
HpaII CCGG 8 cut(s) 259, 298, 306, 336, 387, 518, 653, 914
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 2 cut(s) 247, 379
Hpy188III TCNNGA 3 cut(s) 216, 982, 1016
Hpy8I GTNNAC 1 cut(s) 319
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 1 cut(s) 332
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4V TGCA 9 cut(s) 85, 501, 589, 764, 770, 853, 911, 962, 1007
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 618
HpyF3I CTNAG 1 cut(s) 47
Hsp92II CATG 4 cut(s) 14, 183, 497, 626
HspAI GCGC 1 cut(s) 94
KpnI GGTACC 1 cut(s) 645
KroI GCCGGC 1 cut(s) 305
KroNI GCCGGC 1 cut(s) 307
KspI CCGCGG 1 cut(s) 295
Kzo9I GATC 3 cut(s) 103, 655, 898
Lsp1109I GCAGC 3 cut(s) 69, 601, 946
LweI GCATC 5 cut(s) 451, 510, 822, 898, 1016
MabI ACCWGGT 1 cut(s) 924
MaeI CTAG 1 cut(s) 845
MaeIII GTNAC 8 cut(s) 182, 200, 252, 389, 685, 824, 840, 975
MalI GATC 3 cut(s) 105, 657, 900
MboI GATC 3 cut(s) 103, 655, 898
MboII GAAGA 6 cut(s) 367, 471, 664, 765, 805, 928
MflI RGATCY 1 cut(s) 655
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 4 cut(s) 87, 397, 615, 990
MlyI GAGTC 4 cut(s) 221, 251, 392, 848
MmeI TCCRAC 3 cut(s) 225, 402, 721
Mph1103I ATGCAT 2 cut(s) 503, 1009
MroNI GCCGGC 1 cut(s) 305
MseI TTAA 3 cut(s) 597, 660, 714
MslI CAYNNNNRTG 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 294
MspCI CTTAAG 1 cut(s) 659
MspI CCGG 8 cut(s) 259, 298, 306, 336, 387, 518, 653, 914
MspR9I CCNGG 5 cut(s) 299, 337, 519, 750, 926
Mva1269I GAATGC 3 cut(s) 111, 503, 728
MvaI CCWGG 2 cut(s) 750, 926
MvnI CGCG 2 cut(s) 96, 294
MwoI GCNNNNNNNGC 2 cut(s) 91, 618
NaeI GCCGGC 1 cut(s) 307
NciI CCSGG 3 cut(s) 299, 337, 519
NdeI CATATG 1 cut(s) 76
NdeII GATC 3 cut(s) 103, 655, 898
NgoMIV GCCGGC 1 cut(s) 305
NlaIII CATG 4 cut(s) 14, 183, 497, 626
NlaIV GGNNCC 2 cut(s) 454, 643
NmeAIII GCCGAG 2 cut(s) 135, 342
NmuCI GTSAC 6 cut(s) 182, 200, 252, 685, 824, 840
NsiI ATGCAT 2 cut(s) 503, 1009
PaeR7I CTCGAG 1 cut(s) 982
PctI GAATGC 3 cut(s) 111, 503, 728
PdiI GCCGGC 1 cut(s) 307
PfeI GAWTC 3 cut(s) 270, 358, 636
PfoI TCCNGGA 2 cut(s) 517, 748
PkrI GCNGC 5 cut(s) 84, 296, 304, 591, 961
PleI GAGTC 4 cut(s) 220, 250, 391, 847
PpsI GAGTC 4 cut(s) 220, 250, 391, 847
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 2 cut(s) 748, 924
PspEI GGTNACC 1 cut(s) 389
PspGI CCWGG 2 cut(s) 748, 924
PspN4I GGNNCC 2 cut(s) 454, 643
PspPI GGNCC 3 cut(s) 232, 300, 330
PstI CTGCAG 1 cut(s) 964
PsuI RGATCY 1 cut(s) 655
RsaI GTAC 3 cut(s) 266, 489, 643
RsaNI GTAC 3 cut(s) 265, 488, 642
RseI CAYNNNNRTG 1 cut(s) 75
Rsr2I CGGWCCG 1 cut(s) 232
RsrII CGGWCCG 1 cut(s) 232
SacI GAGCTC 1 cut(s) 225
SacII CCGCGG 1 cut(s) 295
SaqAI TTAA 3 cut(s) 597, 660, 714
SatI GCNGC 5 cut(s) 83, 295, 303, 590, 960
Sau3AI GATC 3 cut(s) 103, 655, 898
Sau96I GGNCC 3 cut(s) 232, 300, 330
SchI GAGTC 4 cut(s) 221, 251, 392, 848
ScrFI CCNGG 5 cut(s) 299, 337, 519, 750, 926
SduI GDGCHC 1 cut(s) 225
SexAI ACCWGGT 1 cut(s) 924
SfaNI GCATC 5 cut(s) 451, 510, 822, 898, 1016
SfcI CTRYAG 1 cut(s) 960
Sfr274I CTCGAG 1 cut(s) 982
Sfr303I CCGCGG 1 cut(s) 295
SgrBI CCGCGG 1 cut(s) 295
SinI GGWCC 1 cut(s) 232
SlaI CTCGAG 1 cut(s) 982
SmiMI CAYNNNNRTG 1 cut(s) 75
SmlI CTYRAG 3 cut(s) 659, 836, 982
SmoI CTYRAG 3 cut(s) 659, 836, 982
Sse9I AATT 4 cut(s) 87, 397, 615, 990
SsiI CCGC 7 cut(s) 68, 292, 294, 303, 333, 464, 549
SspMI CTAG 1 cut(s) 845
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 5 cut(s) 297, 335, 517, 748, 924
StyI CCWWGG 1 cut(s) 456
TaaI ACNGT 1 cut(s) 151
TaqI TCGA 3 cut(s) 59, 816, 983
TaqII GACCGA 1 cut(s) 220
TasI AATT 4 cut(s) 87, 397, 615, 990
TauI GCSGC 2 cut(s) 297, 305
TfiI GAWTC 3 cut(s) 270, 358, 636
Tru1I TTAA 3 cut(s) 597, 660, 714
Tru9I TTAA 3 cut(s) 597, 660, 714
TscAI CASTG 1 cut(s) 356
TseFI GTSAC 6 cut(s) 182, 200, 252, 685, 824, 840
TseI GCWGC 3 cut(s) 82, 589, 959
Tsp45I GTSAC 6 cut(s) 182, 200, 252, 685, 824, 840
TspDTI ATGAA 5 cut(s) 403, 471, 510, 735, 834
TspGWI ACGGA 2 cut(s) 224, 304
TspRI CASTG 1 cut(s) 356
Vha464I CTTAAG 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 232
XagI CCTNNNNNAGG 1 cut(s) 606
XapI RAATTY 2 cut(s) 87, 615
XcmI CCANNNNNNNNNTGG 2 cut(s) 454, 737
XhoI CTCGAG 1 cut(s) 982
XmiI GTMKAC 1 cut(s) 318
XspI CTAG 1 cut(s) 845
Zsp2I ATGCAT 2 cut(s) 503, 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.