Rorug06G0278400

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001445
Physical Location & Seq
Reverse (-)
42603503 .. 42604812
1310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0278400.1

Sequence Viewer

Length: 942 bp
ATGAAATCCCAAAAGAAAAGGACGGCTATTCTAGTTGATATCTTTCTTGTCCTGCAGTTCTTGATGATCTCTCTGGTTCAAGGCGAAGTACATTTCTACGATTTTGTATTAAGGGAGAAAAACTTCACCAGATTATGCGAAACAAAGAGCATGTTTGTTGTAAACGACATTTTCCCAGGCCCGGAGATACGAGTTCATAAAGGGGATATGGTCTATGTAAATGTTTATAATCAAGGATATTATGGTCTCACTATTCACTGGCATGGAATAATGCAACTAAGAAATCCATGGTCAGATGGTCCTGAGTATATCACACAGTGTCCAATCCAACCAGGGACTAATTTCACATACCAGGTCTTGTTATCTACGGAAGAAGGAACAGTATGGTGGCATGCTCATAGTGACTGGACACGAGCTAGCGTTCATGGGGCCATTGTCATCTTGCCTACTGTTGGAAGCACATATCCATTTCCTCAACCCGATGAAGATGAGGTCATTATATTATCATCCTGGTACTTAGGAGATTTGAAAGTAAGGGTTGATGAGGCTATGAAAGAAGCCACATCTTTACCCCGTTCTGATGCATACACCATAAATGGGCAGCCGGGGGATCTTTTTCCATGCTCCAAGGGGACAGCATATCGTCGAAAAGTGGATTATGCCAAGACCTATCTTCTTCGTATAGTCAATGCAAACATAGATTCATCCCTTTTCTTTGCAGTTTCTCAACACAACCTCACTGTTGTCGGACTAGACGGAGCCTATATCAAACCCATATACACAACCTACATAGTCATAAGCCCTGGACAAACTATGGATGTTTTACTCAAAGCAGATCAGGTTCTCGGGCGCTATTACATGGCTGGTAGACATTTCTCCAGTGATAATGCCAAAGTTTCTGAATTTGGCCATGATGTTACTGCAATCTTGGAATACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

313

Amino Acids

35.6

Weight (kDa)

6.05

Isoelectric Point (pI)

30.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 38 - 149 3.1e-37 Multicopper oxidase
Cu-oxidase PF00394 163 - 313 1.5e-31 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
AccI GTMKAC 1 cut(s) 868
AclWI GGATC 1 cut(s) 618
AcoI YGGCCR 1 cut(s) 907
AcsI RAATTY 1 cut(s) 902
AdeI CACNNNGTG 1 cut(s) 318
AfaI GTAC 2 cut(s) 90, 515
AfiI CCNNNNNNNGG 3 cut(s) 181, 452, 597
AgsI TTSAA 2 cut(s) 80, 529
AjnI CCWGG 5 cut(s) 175, 331, 351, 509, 802
AluBI AGCT 1 cut(s) 416
AluI AGCT 1 cut(s) 416
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 1 cut(s) 618
Ama87I CYCGRG 1 cut(s) 845
AoxI GGCC 3 cut(s) 178, 429, 907
ApeKI GCWGC 1 cut(s) 601
ApoI RAATTY 1 cut(s) 902
Asp700I GAANNNNTTC 1 cut(s) 122
AspLEI GCGC 1 cut(s) 852
AspS9I GGNCC 3 cut(s) 179, 299, 429
AsuC2I CCSGG 2 cut(s) 182, 606
AsuHPI GGTGA 1 cut(s) 118
AsuNHI GCTAGC 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 845
AvaII GGWCC 1 cut(s) 299
BalI TGGCCA 1 cut(s) 909
BauI CACGAG 1 cut(s) 411
BbvI GCAGC 1 cut(s) 613
BccI CCATC 1 cut(s) 290
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 5 cut(s) 177, 333, 353, 511, 804
BcnI CCSGG 2 cut(s) 182, 606
BcoDI GTCTC 1 cut(s) 251
BfaI CTAG 3 cut(s) 32, 417, 752
BfmI CTRYAG 1 cut(s) 53
BfoI RGCGCY 1 cut(s) 853
BisI GCNGC 1 cut(s) 602
BlsI GCNGC 1 cut(s) 603
Bme1390I CCNGG 7 cut(s) 177, 182, 333, 353, 511, 606, 804
Bme18I GGWCC 1 cut(s) 299
BmeT110I CYCGRG 1 cut(s) 845
BmgT120I GGNCC 3 cut(s) 179, 299, 429
BmiI GGNNCC 2 cut(s) 430, 760
BmrFI CCNGG 7 cut(s) 177, 182, 333, 353, 511, 606, 804
BmsI GCATC 1 cut(s) 571
BmtI GCTAGC 1 cut(s) 420
BpmI CTGGAG 1 cut(s) 862
BpuMI CCSGG 2 cut(s) 182, 606
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 6 cut(s) 175, 287, 332, 605, 627, 802
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 452, 597
Bse1I ACTGG 3 cut(s) 263, 410, 879
BseBI CCWGG 5 cut(s) 177, 333, 353, 511, 804
BseDI CCNNGG 6 cut(s) 175, 287, 332, 605, 627, 802
BseGI GGATG 3 cut(s) 506, 704, 823
BseLI CCNNNNNNNGG 3 cut(s) 181, 452, 597
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 3 cut(s) 263, 410, 879
BseXI GCAGC 1 cut(s) 613
BshFI GGCC 3 cut(s) 180, 431, 909
BsiHKCI CYCGRG 1 cut(s) 845
BsiSI CCGG 2 cut(s) 182, 605
BslFI GGGAC 2 cut(s) 349, 646
BslI CCNNNNNNNGG 3 cut(s) 181, 452, 597
BsmAI GTCTC 1 cut(s) 251
BsmFI GGGAC 2 cut(s) 349, 646
BsnI GGCC 3 cut(s) 180, 431, 909
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 845
Bsp143I GATC 3 cut(s) 66, 610, 835
Bsp19I CCATGG 1 cut(s) 287
BspANI GGCC 3 cut(s) 180, 431, 909
BspCNI CTCAG 1 cut(s) 295
BspLI GGNNCC 2 cut(s) 430, 760
BspMAI CTGCAG 1 cut(s) 57
BspOI GCTAGC 1 cut(s) 420
BspPI GGATC 1 cut(s) 618
BspTNI GGTCTC 1 cut(s) 251
BsrI ACTGG 3 cut(s) 263, 410, 879
BssECI CCNNGG 6 cut(s) 175, 287, 332, 605, 627, 802
BssMI GATC 3 cut(s) 66, 610, 835
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 2 cut(s) 287, 627
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 5 cut(s) 177, 333, 353, 511, 804
Bst4CI ACNGT 4 cut(s) 318, 382, 451, 742
BstC8I GCNNGC 2 cut(s) 393, 418
BstDEI CTNAG 3 cut(s) 278, 303, 517
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 3 cut(s) 506, 704, 823
BstH2I RGCGCY 1 cut(s) 853
BstHHI GCGC 1 cut(s) 852
BstKTI GATC 3 cut(s) 69, 613, 838
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 3 cut(s) 66, 610, 835
BstNI CCWGG 5 cut(s) 177, 333, 353, 511, 804
BstNSI RCATGY 2 cut(s) 154, 395
BstSCI CCNGG 7 cut(s) 175, 180, 331, 351, 509, 604, 802
BstSFI CTRYAG 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 613
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BsuRI GGCC 3 cut(s) 180, 431, 909
BtgI CCRYGG 1 cut(s) 287
BtsCI GGATG 3 cut(s) 506, 704, 823
BtsIMutI CAGTG 4 cut(s) 256, 323, 738, 886
Cac8I GCNNGC 2 cut(s) 393, 418
CfoI GCGC 1 cut(s) 852
Cfr13I GGNCC 3 cut(s) 179, 299, 429
CsiI ACCWGGT 1 cut(s) 351
Csp6I GTAC 2 cut(s) 89, 514
CviAII CATG 8 cut(s) 151, 263, 288, 392, 425, 621, 859, 911
CviQI GTAC 2 cut(s) 89, 514
DdeI CTNAG 3 cut(s) 278, 303, 517
DpnI GATC 3 cut(s) 68, 612, 837
DpnII GATC 3 cut(s) 66, 610, 835
DraIII CACNNNGTG 1 cut(s) 318
EaeI YGGCCR 1 cut(s) 907
Eco130I CCWWGG 2 cut(s) 287, 627
Eco31I GGTCTC 1 cut(s) 251
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 845
EcoRII CCWGG 5 cut(s) 175, 331, 351, 509, 802
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 2 cut(s) 287, 627
EcoT22I ATGCAT 1 cut(s) 586
ErhI CCWWGG 2 cut(s) 287, 627
FaeI CATG 8 cut(s) 154, 266, 291, 395, 428, 624, 862, 914
FaqI GGGAC 2 cut(s) 349, 646
FatI CATG 8 cut(s) 150, 262, 287, 391, 424, 620, 858, 910
FblI GTMKAC 1 cut(s) 868
Fnu4HI GCNGC 1 cut(s) 602
FokI GGATG 3 cut(s) 493, 691, 830
Fsp4HI GCNGC 1 cut(s) 602
FspBI CTAG 3 cut(s) 32, 417, 752
GlaI GCGC 1 cut(s) 851
GluI GCNGC 1 cut(s) 602
GsuI CTGGAG 1 cut(s) 862
HaeII RGCGCY 1 cut(s) 853
HaeIII GGCC 3 cut(s) 180, 431, 909
HapII CCGG 2 cut(s) 182, 605
HhaI GCGC 1 cut(s) 852
Hin1II CATG 8 cut(s) 154, 266, 291, 395, 428, 624, 862, 914
Hin6I GCGC 1 cut(s) 850
HinP1I GCGC 1 cut(s) 850
HinfI GANTC 1 cut(s) 701
HpaII CCGG 2 cut(s) 182, 605
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 2 cut(s) 163, 869
Hpy188I TCNGA 4 cut(s) 295, 580, 749, 901
Hpy188III TCNNGA 2 cut(s) 61, 302
Hpy8I GTNNAC 2 cut(s) 163, 869
Hpy99I CGWCG 1 cut(s) 648
HpyAV CCTTC 1 cut(s) 368
HpyCH4III ACNGT 4 cut(s) 318, 382, 451, 742
HpyCH4V TGCA 6 cut(s) 55, 274, 584, 692, 719, 923
HpyF3I CTNAG 3 cut(s) 278, 303, 517
Hsp92II CATG 8 cut(s) 154, 266, 291, 395, 428, 624, 862, 914
HspAI GCGC 1 cut(s) 850
Kzo9I GATC 3 cut(s) 66, 610, 835
LmnI GCTCC 2 cut(s) 629, 758
Lsp1109I GCAGC 1 cut(s) 613
LweI GCATC 1 cut(s) 571
MabI ACCWGGT 1 cut(s) 351
MaeI CTAG 3 cut(s) 32, 417, 752
MaeIII GTNAC 2 cut(s) 401, 916
MalI GATC 3 cut(s) 68, 612, 837
MboI GATC 3 cut(s) 66, 610, 835
MboII GAAGA 4 cut(s) 383, 497, 665, 668
MflI RGATCY 1 cut(s) 610
MlsI TGGCCA 1 cut(s) 909
MluCI AATT 3 cut(s) 340, 902, 937
MluNI TGGCCA 1 cut(s) 909
MmeI TCCRAC 3 cut(s) 352, 433, 727
MnlI CCTC 4 cut(s) 483, 484, 538, 746
Mox20I TGGCCA 1 cut(s) 909
Mph1103I ATGCAT 1 cut(s) 586
MroXI GAANNNNTTC 1 cut(s) 122
MscI TGGCCA 1 cut(s) 909
MseI TTAA 2 cut(s) 110, 940
MslI CAYNNNNRTG 1 cut(s) 261
Msp20I TGGCCA 1 cut(s) 909
MspI CCGG 2 cut(s) 182, 605
MspR9I CCNGG 7 cut(s) 177, 182, 333, 353, 511, 606, 804
MvaI CCWGG 5 cut(s) 177, 333, 353, 511, 804
NciI CCSGG 2 cut(s) 182, 606
NcoI CCATGG 1 cut(s) 287
NdeII GATC 3 cut(s) 66, 610, 835
NheI GCTAGC 1 cut(s) 416
NlaIII CATG 8 cut(s) 154, 266, 291, 395, 428, 624, 862, 914
NlaIV GGNNCC 2 cut(s) 430, 760
NmuCI GTSAC 1 cut(s) 401
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 2 cut(s) 154, 395
PaeI GCATGC 1 cut(s) 395
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 1 cut(s) 701
PkrI GCNGC 1 cut(s) 603
PsiI TTATAA 1 cut(s) 228
Psp6I CCWGG 5 cut(s) 175, 331, 351, 509, 802
PspGI CCWGG 5 cut(s) 175, 331, 351, 509, 802
PspN4I GGNNCC 2 cut(s) 430, 760
PspPI GGNCC 3 cut(s) 179, 299, 429
PstI CTGCAG 1 cut(s) 57
PsuI RGATCY 1 cut(s) 610
RsaI GTAC 2 cut(s) 90, 515
RsaNI GTAC 2 cut(s) 89, 514
RseI CAYNNNNRTG 1 cut(s) 261
SaqAI TTAA 2 cut(s) 110, 940
SatI GCNGC 1 cut(s) 602
Sau3AI GATC 3 cut(s) 66, 610, 835
Sau96I GGNCC 3 cut(s) 179, 299, 429
ScrFI CCNGG 7 cut(s) 177, 182, 333, 353, 511, 606, 804
SetI ASST 7 cut(s) 357, 418, 495, 671, 738, 788, 843
SexAI ACCWGGT 1 cut(s) 351
SfaNI GCATC 1 cut(s) 571
SfcI CTRYAG 1 cut(s) 53
SinI GGWCC 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 261
SphI GCATGC 1 cut(s) 395
Sse9I AATT 3 cut(s) 340, 902, 937
SspMI CTAG 3 cut(s) 32, 417, 752
StyD4I CCNGG 7 cut(s) 175, 180, 331, 351, 509, 604, 802
StyI CCWWGG 2 cut(s) 287, 627
TaaI ACNGT 4 cut(s) 318, 382, 451, 742
TaqI TCGA 1 cut(s) 646
TasI AATT 3 cut(s) 340, 902, 937
TatI WGTACW 1 cut(s) 88
TfiI GAWTC 1 cut(s) 701
Tru1I TTAA 2 cut(s) 110, 940
Tru9I TTAA 2 cut(s) 110, 940
TscAI CASTG 4 cut(s) 263, 323, 745, 886
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 1 cut(s) 601
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 6 cut(s) 17, 185, 413, 498, 566, 693
TspGWI ACGGA 2 cut(s) 383, 771
TspRI CASTG 4 cut(s) 263, 323, 745, 886
VpaK11BI GGWCC 1 cut(s) 299
XapI RAATTY 1 cut(s) 902
XceI RCATGY 2 cut(s) 154, 395
XmiI GTMKAC 1 cut(s) 868
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 3 cut(s) 32, 417, 752
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.