MD00G1022900.v1.1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0001445
Physical Location & Seq
Reverse (-)
3860265 .. 3866862
6598 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1022900.v1.1.491

Sequence Viewer

Length: 1287 bp
ATGGGTGAAGTCAGAGACAACGATGGGTACGAAGAGGAGCTCGTCGACTACGAAGAGGAGGACCAAAACGCCCCCAACTCCGTCTCTGCCAAGCCTTCTGGCGATACCGTCAAAAAGGGATATGTTGGCATCCATAGTTCGGGATTCAGAGACTTCCTGTTGAAGCCGGAGCTTCTTCGAGCAATTGTGGATTCTGGATTCGAACATCCTTCGGAAGTTCAACATGAGTGCATCCCTCAAGCTATTTTAGGAATGGATGTTATTTGTCAAGCAAAATCTGGGATGGGAAAAACTGCCGTCTTTGTTCTGTCAACCCTTCAGCAAATAGATCCAGTAGCTGGTCAGGTTGCTGCTCTTATTCTTTGCCACACGAGAGAATTGGCTTACCAGATTTGTCATGAATTTGAGAGGTTTAGTACTTATTTGCCGGACCTGAAGGTTGCAGTTTTCTATGGAGGTGTTAGTATCAAGGTTCACAAAGATTTGCTAAAAAATGAATGTCCTCACATTGTCGTTGGTACACCTGGGAGAGTATTAGCTTTGGCAAGAGACAAAGAGCTTGGACTGAAGAATGTCAGACACTTTATTCTTGATGAATGCGATAAGATGCTTGAGTCACTTGACATGAGGAGAGATGTGCAGGAGATATTTAAGTTGACTCCTCATGACAAGCAAGTGATGATGTTTTCAGCAACACTAAGCAAGGAAATACGCCCTGTTTGCAAGAAGTTTATGCAAGATCCCATGGAAATTTATGTGGATGATGAGGCCAAATTGACTCTTCATGGTCTCGTACAGCACTACATCAAATTGAGTGAGCCTGAGAAAAACCGCAAGTTGACTGACCTCCTTGATGCATTGGATTTCAACCAAGTTGTTATTTTTGTAAAGAGTGTAAGCAGGGCAGCTGAGCTGAACAAGTTACTTGCTGACTGTAACTTCCCCTCCATCTGCATTCACTCTGGAATGCCTCAGGAAGAAAGGCTAAAACGTTACAAGAATTTCAAGGAGGGGCTTTCAAGAATTCTTGTGGCAACAGATTTAGTAGGAAGGGGAATTGACATTGAGCGTGTTAACATTGTTATCAATTATGACATGCCTGATTCTGCTGATACCTACTTGCACAGGGTTGGCAGAGCTGGTAGGTTTGGTACCAAGGGGCTAGCAATTACATTTGTATCTTCTGCATCTGATTCAGAAGTTCTTAATCAGGTCCAGTCAAGGTTTGAGGTGGACATAAAGGAGCTTCCCGAGCAAATTGATACTTCTACTTACATGCAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006974 GO:0007275 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009555 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:1901360 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

429

Amino Acids

48.22

Weight (kDa)

5.47

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 72 - 237 3e-40 DEAD/DEAH box helicase
ResIII PF04851 87 - 234 9.9e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 276 - 384 9.9e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1151
AccB1I GGYRCC 1 cut(s) 1151
AccB7I CCANNNNNTGG 1 cut(s) 338
AccI GTMKAC 1 cut(s) 45
AciI CCGC 1 cut(s) 832
AclI AACGTT 1 cut(s) 991
AclWI GGATC 2 cut(s) 323, 734
AcsI RAATTY 4 cut(s) 401, 750, 1000, 1023
AcuI CTGAAG 3 cut(s) 302, 455, 587
AfaI GTAC 5 cut(s) 29, 418, 520, 795, 1153
AfiI CCNNNNNNNGG 2 cut(s) 139, 338
AgsI TTSAA 5 cut(s) 163, 221, 868, 1006, 1020
AjnI CCWGG 1 cut(s) 523
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 5 cut(s) 9, 88, 144, 543, 794
AlwI GGATC 2 cut(s) 323, 734
AlwNI CAGNNNCTG 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 1250
AoxI GGCC 1 cut(s) 768
ApeKI GCWGC 2 cut(s) 350, 905
ApoI RAATTY 4 cut(s) 401, 750, 1000, 1023
Asp718I GGTACC 1 cut(s) 1151
AspS9I GGNCC 3 cut(s) 61, 430, 1213
AsuHPI GGTGA 1 cut(s) 17
AsuII TTCGAA 1 cut(s) 201
AsuNHI GCTAGC 1 cut(s) 1162
AvaI CYCGRG 1 cut(s) 1250
AvaII GGWCC 3 cut(s) 61, 430, 1213
AxyI CCTNAGG 1 cut(s) 972
BaeI ACNNNNGTAYC 2 cut(s) 1161, 1194
BanI GGYRCC 1 cut(s) 1151
BanII GRGCYC 1 cut(s) 42
BauI CACGAG 1 cut(s) 370
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 2 cut(s) 337, 917
BccI CCATC 3 cut(s) 17, 277, 956
BceAI ACGGC 1 cut(s) 281
BciT130I CCWGG 1 cut(s) 525
BcoDI GTCTC 5 cut(s) 9, 88, 144, 543, 794
BfaI CTAG 1 cut(s) 1163
BisI GCNGC 2 cut(s) 351, 906
BlpI GCTNAGC 1 cut(s) 909
BlsI GCNGC 2 cut(s) 352, 907
BmcAI AGTACT 1 cut(s) 418
Bme1390I CCNGG 1 cut(s) 525
Bme18I GGWCC 3 cut(s) 61, 430, 1213
BmeT110I CYCGRG 1 cut(s) 1250
BmgT120I GGNCC 3 cut(s) 61, 430, 1213
BmiI GGNNCC 1 cut(s) 1153
BmrFI CCNGG 1 cut(s) 525
BmsI GCATC 5 cut(s) 138, 240, 597, 844, 1196
BmtI GCTAGC 1 cut(s) 1166
Bpu1102I GCTNAGC 1 cut(s) 909
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 222, 632
BsaI GGTCTC 1 cut(s) 794
BsaJI CCNNGG 3 cut(s) 524, 744, 1155
BsaXI ACNNNNNCTCC 2 cut(s) 929, 959
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 338
Bse1I ACTGG 2 cut(s) 332, 1216
Bse21I CCTNAGG 1 cut(s) 972
BseBI CCWGG 1 cut(s) 525
BseDI CCNNGG 3 cut(s) 524, 744, 1155
BseGI GGATG 6 cut(s) 129, 205, 231, 262, 288, 766
BseLI CCNNNNNNNGG 2 cut(s) 139, 338
BseMII CTCAG 3 cut(s) 813, 900, 986
BseNI ACTGG 2 cut(s) 332, 1216
BseRI GAGGAG 4 cut(s) 50, 71, 643, 651
BseXI GCAGC 2 cut(s) 337, 917
BsgI GTGCAG 1 cut(s) 659
BshFI GGCC 1 cut(s) 770
BshNI GGYRCC 1 cut(s) 1151
BsiHKAI GWGCWC 1 cut(s) 42
BsiHKCI CYCGRG 1 cut(s) 1250
BsiSI CCGG 2 cut(s) 167, 428
BslI CCNNNNNNNGG 2 cut(s) 139, 338
BsmAI GTCTC 5 cut(s) 9, 88, 144, 543, 794
BsmBI CGTCTC 1 cut(s) 88
BsmI GAATGC 3 cut(s) 602, 954, 972
BsnI GGCC 1 cut(s) 770
Bso31I GGTCTC 1 cut(s) 794
BsoBI CYCGRG 1 cut(s) 1250
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 2 cut(s) 328, 739
Bsp1720I GCTNAGC 1 cut(s) 909
Bsp19I CCATGG 1 cut(s) 744
BspACI CCGC 1 cut(s) 832
BspANI GGCC 1 cut(s) 770
BspCNI CTCAG 3 cut(s) 814, 901, 985
BspHI TCATGA 2 cut(s) 397, 664
BspLI GGNNCC 1 cut(s) 1153
BspOI GCTAGC 1 cut(s) 1166
BspPI GGATC 2 cut(s) 323, 734
BspT104I TTCGAA 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 1151
BspTNI GGTCTC 1 cut(s) 794
BsrI ACTGG 2 cut(s) 332, 1216
BssECI CCNNGG 3 cut(s) 524, 744, 1155
BssMI GATC 2 cut(s) 328, 739
BssSI CACGAG 1 cut(s) 370
BssT1I CCWWGG 2 cut(s) 744, 1155
Bst2BI CACGAG 1 cut(s) 370
Bst2UI CCWGG 1 cut(s) 525
Bst4CI ACNGT 2 cut(s) 109, 935
Bst6I CTCTTC 3 cut(s) 27, 48, 786
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 1164
BstDEI CTNAG 4 cut(s) 698, 822, 909, 972
BstDSI CCRYGG 1 cut(s) 744
BstF5I GGATG 6 cut(s) 129, 205, 231, 262, 288, 766
BstKTI GATC 2 cut(s) 331, 742
BstMAI GTCTC 5 cut(s) 9, 88, 144, 543, 794
BstMBI GATC 2 cut(s) 328, 739
BstMWI GCNNNNNNNGC 2 cut(s) 720, 1252
BstNI CCWGG 1 cut(s) 525
BstNSI RCATGY 2 cut(s) 1099, 1279
BstSCI CCNGG 1 cut(s) 523
BstV1I GCAGC 2 cut(s) 337, 917
BstX2I RGATCY 2 cut(s) 328, 739
BstYI RGATCY 2 cut(s) 328, 739
Bsu36I CCTNAGG 1 cut(s) 972
BsuRI GGCC 1 cut(s) 770
BtgI CCRYGG 1 cut(s) 744
BtsCI GGATG 6 cut(s) 129, 205, 231, 262, 288, 766
Cac8I GCNNGC 1 cut(s) 1164
CaiI CAGNNNCTG 1 cut(s) 338
CciI TCATGA 2 cut(s) 397, 664
Cfr13I GGNCC 3 cut(s) 61, 430, 1213
Csp6I GTAC 5 cut(s) 28, 417, 519, 794, 1152
CviAII CATG 8 cut(s) 224, 398, 625, 665, 745, 785, 1096, 1276
CviQI GTAC 5 cut(s) 28, 417, 519, 794, 1152
DdeI CTNAG 4 cut(s) 698, 822, 909, 972
DpnI GATC 2 cut(s) 330, 741
DpnII GATC 2 cut(s) 328, 739
Eam1104I CTCTTC 3 cut(s) 27, 48, 786
EarI CTCTTC 3 cut(s) 27, 48, 786
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 2 cut(s) 744, 1155
Eco24I GRGCYC 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 794
Eco47I GGWCC 3 cut(s) 61, 430, 1213
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 3 cut(s) 302, 455, 587
Eco81I CCTNAGG 1 cut(s) 972
Eco88I CYCGRG 1 cut(s) 1250
EcoICRI GAGCTC 1 cut(s) 40
EcoRI GAATTC 1 cut(s) 1023
EcoRII CCWGG 1 cut(s) 523
EcoT14I CCWWGG 2 cut(s) 744, 1155
EcoT22I ATGCAT 1 cut(s) 859
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 2 cut(s) 744, 1155
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 8 cut(s) 227, 401, 628, 668, 748, 788, 1099, 1279
FatI CATG 8 cut(s) 223, 397, 624, 664, 744, 784, 1095, 1275
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 2 cut(s) 351, 906
FokI GGATG 6 cut(s) 116, 192, 218, 269, 295, 773
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 2 cut(s) 351, 906
FspBI CTAG 1 cut(s) 1163
GluI GCNGC 2 cut(s) 351, 906
HaeIII GGCC 1 cut(s) 770
HapII CCGG 2 cut(s) 167, 428
Hin1II CATG 8 cut(s) 227, 401, 628, 668, 748, 788, 1099, 1279
HincII GTYRAC 5 cut(s) 46, 312, 657, 840, 1075
HindII GTYRAC 5 cut(s) 46, 312, 657, 840, 1075
HinfI GANTC 8 cut(s) 144, 191, 198, 614, 658, 778, 1103, 1193
HpaI GTTAAC 1 cut(s) 1075
HpaII CCGG 2 cut(s) 167, 428
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 8 cut(s) 46, 312, 475, 521, 657, 840, 1075, 1234
Hpy188I TCNGA 6 cut(s) 14, 149, 214, 578, 1192, 1198
Hpy188III TCNNGA 9 cut(s) 141, 195, 398, 590, 665, 963, 974, 1020, 1250
Hpy8I GTNNAC 8 cut(s) 46, 312, 475, 521, 657, 840, 1075, 1234
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 5 cut(s) 105, 219, 326, 430, 1044
HpyCH4III ACNGT 2 cut(s) 109, 935
HpyCH4IV ACGT 1 cut(s) 991
HpyF10VI GCNNNNNNNGC 2 cut(s) 720, 1252
HpyF3I CTNAG 4 cut(s) 698, 822, 909, 972
HpySE526I ACGT 1 cut(s) 991
Hsp92II CATG 8 cut(s) 227, 401, 628, 668, 748, 788, 1099, 1279
KpnI GGTACC 1 cut(s) 1155
KspAI GTTAAC 1 cut(s) 1075
Kzo9I GATC 2 cut(s) 328, 739
LmnI GCTCC 3 cut(s) 37, 169, 1243
Lsp1109I GCAGC 2 cut(s) 337, 917
LweI GCATC 5 cut(s) 138, 240, 597, 844, 1196
MaeI CTAG 1 cut(s) 1163
MaeII ACGT 1 cut(s) 991
MaeIII GTNAC 4 cut(s) 615, 921, 935, 992
MalI GATC 2 cut(s) 330, 741
MboI GATC 2 cut(s) 328, 739
MboII GAAGA 7 cut(s) 44, 65, 167, 580, 773, 989, 1173
MfeI CAATTG 1 cut(s) 183
MflI RGATCY 2 cut(s) 328, 739
MhlI GDGCHC 1 cut(s) 42
MlyI GAGTC 3 cut(s) 623, 652, 772
Mph1103I ATGCAT 1 cut(s) 859
MseI TTAA 3 cut(s) 651, 1074, 1206
MslI CAYNNNNRTG 1 cut(s) 1280
MspA1I CMGCKG 1 cut(s) 908
MspI CCGG 2 cut(s) 167, 428
MspR9I CCNGG 1 cut(s) 525
MunI CAATTG 1 cut(s) 183
Mva1269I GAATGC 3 cut(s) 602, 954, 972
MvaI CCWGG 1 cut(s) 525
MwoI GCNNNNNNNGC 2 cut(s) 720, 1252
NcoI CCATGG 1 cut(s) 744
NdeII GATC 2 cut(s) 328, 739
NheI GCTAGC 1 cut(s) 1162
NlaIII CATG 8 cut(s) 227, 401, 628, 668, 748, 788, 1099, 1279
NlaIV GGNNCC 1 cut(s) 1153
NmuCI GTSAC 1 cut(s) 615
NsiI ATGCAT 1 cut(s) 859
NspI RCATGY 2 cut(s) 1099, 1279
NspV TTCGAA 1 cut(s) 201
PagI TCATGA 2 cut(s) 397, 664
PcsI WCGNNNNNNNCGW 2 cut(s) 27, 48
PctI GAATGC 3 cut(s) 602, 954, 972
PfeI GAWTC 5 cut(s) 144, 191, 198, 1103, 1193
PflMI CCANNNNNTGG 1 cut(s) 338
PkrI GCNGC 2 cut(s) 352, 907
PleI GAGTC 3 cut(s) 622, 652, 772
PpsI GAGTC 3 cut(s) 622, 652, 772
Psp124BI GAGCTC 1 cut(s) 42
Psp1406I AACGTT 1 cut(s) 991
Psp6I CCWGG 1 cut(s) 523
PspGI CCWGG 1 cut(s) 523
PspN4I GGNNCC 1 cut(s) 1153
PspPI GGNCC 3 cut(s) 61, 430, 1213
PstNI CAGNNNCTG 1 cut(s) 338
PsuI RGATCY 2 cut(s) 328, 739
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 5 cut(s) 29, 418, 520, 795, 1153
RsaNI GTAC 5 cut(s) 28, 417, 519, 794, 1152
RseI CAYNNNNRTG 1 cut(s) 1280
SacI GAGCTC 1 cut(s) 42
SalI GTCGAC 1 cut(s) 44
SaqAI TTAA 3 cut(s) 651, 1074, 1206
SatI GCNGC 2 cut(s) 351, 906
Sau3AI GATC 2 cut(s) 328, 739
Sau96I GGNCC 3 cut(s) 61, 430, 1213
ScaI AGTACT 1 cut(s) 418
SchI GAGTC 3 cut(s) 623, 652, 772
ScrFI CCNGG 1 cut(s) 525
SduI GDGCHC 1 cut(s) 42
SfaNI GCATC 5 cut(s) 138, 240, 597, 844, 1196
SfuI TTCGAA 1 cut(s) 201
SinI GGWCC 3 cut(s) 61, 430, 1213
SmiMI CAYNNNNRTG 1 cut(s) 1280
SmlI CTYRAG 2 cut(s) 237, 611
SmoI CTYRAG 2 cut(s) 237, 611
SsiI CCGC 1 cut(s) 832
SspMI CTAG 1 cut(s) 1163
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 523
StyI CCWWGG 2 cut(s) 744, 1155
TaaI ACNGT 2 cut(s) 109, 935
TaiI ACGT 1 cut(s) 994
TaqI TCGA 3 cut(s) 45, 178, 201
TatI WGTACW 1 cut(s) 416
TfiI GAWTC 5 cut(s) 144, 191, 198, 1103, 1193
Tru1I TTAA 3 cut(s) 651, 1074, 1206
Tru9I TTAA 3 cut(s) 651, 1074, 1206
TseFI GTSAC 1 cut(s) 615
TseI GCWGC 2 cut(s) 350, 905
Tsp45I GTSAC 1 cut(s) 615
TspDTI ATGAA 4 cut(s) 414, 510, 609, 773
TspGWI ACGGA 1 cut(s) 70
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 3 cut(s) 61, 430, 1213
XapI RAATTY 4 cut(s) 401, 750, 1000, 1023
XceI RCATGY 2 cut(s) 1099, 1279
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 1 cut(s) 1163
ZrmI AGTACT 1 cut(s) 418
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.