MD08G1154600.v1.1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001445
Physical Location & Seq
Reverse (-)
17181555 .. 17186884
5330 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1154600.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGTCCGCCTCCCGGTTACTGCAACCCTATCTCTCACACTCTCTCCTGCTCTCCTCTTTCGTCGAGAACATGGGCGAAACTAGGGACGACGTCTACGACGAGGAGCTCGTCGATTACGAAGAAGAGGAGGAGAAGGCACCCGACTCCGCAGCTAAAGTCAACGGAGAAGCCCCCAAAAAGGGTTATGTTGGAATTCACAGTTCAGGGTTCAGAGATTTCCTTTTGAAGCCTGAACTTCTTCGTGCTATTGTCGACTCTGGATTTGAGCATCCTTCTGAAGGCAAGCTCTTGAACTTAGACTTTTTGATTGTTATTTTCTTGCGGAAGTCAAACCATGTTATATCTTTTCTTTCCGTTCTGTGCTTAGTTCTAATTCAGTTTGATGTATTTTTTCCAGTGCAACATGAATGTATTCCTCAAGCTATCCTGGGGATGGATGTTATATGTCAGGCTAAATCTGGGATGGGAAAAACTGCTGTGTTTGTTCTATCAACTTTACAACAGATTGACCCTGTTGCAGGCCAAGTATCTGCACTTGTCCTTTGCCATACTAGAGAATTAGCATACCAGATTTGTCATGAATTTGAGAGGTTCAGTACGTACCTGCCTGATCTCAAGGTTGCTGTCTTCTATGGTGGTGTCAGTATCAAACTTCACAAAGATTTACTGAAAAACGAATGCCCGCACATTGTTGTCGGAACTCCTGGAAGAATACTAGCACTGGCAAGAGATAAAGACCTGTCGTTGAAAAACGTGAGGCATTTTATTCTTGATGAATGTGACAAGATGCTTGAATCACTTGACATGCGGAGAGATGTGCAGGAGATCTTCAAGATGACGCCTCACGATAAGCAAGTGATGATGTTTTCCGCAACACTCAGCAAAGACATCCGCCCGGTTTGCAAGAAATTTATGCAAGATCCTATGGAAATATATGTTGATGATGAAGCCAAGTTGACCTTGCATGGTCTAGTGCAGCACTACATCAAGTTAACTGAGGCAGAGAAGAACCGCAAGTTGAATGACCTCCTTGATGCATTGGACTTCAATCAAGTTGTTATCTTCGTCAAAAGCGTTAGCAGAGCTGCTGAGCTGAACAAGTTACTTTCAGAGTGTAACTTTCCCTCTATCTGCATCCATTCTGGAATGAACCAAGAAGAAAGGTTGAAGCGGTACAAAGGATTCAAGGAGGGTCAGAACAGGATTCTTGTGGCAACTGATTTAGTCGGTAGAGGAATTGACATCGAACGTGTTAACATTGTTATCAACTACGACATGCCAGATTCTGCTGACACTTATTTACACAGGGTTGGCAGAGCTGGTAGGTTTGGCACCAAAGGTCTTGCAATTACGTTTGTTTCATCCGCAGCAGACTCTGATGTTCTAAATGATGTTCAGGAAAGGTTTGAGGTTGATATAAAGGAGCTTCCTGAACAGATTGACACTGCCACATACATGCCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

489

Amino Acids

55.17

Weight (kDa)

5.46

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 132 - 297 6.3e-41 DEAD/DEAH box helicase
ResIII PF04851 147 - 294 3.4e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 336 - 444 8.6e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
Acc36I ACCTGC 1 cut(s) 612
AccB1I GGYRCC 2 cut(s) 136, 1331
AccI GTMKAC 2 cut(s) 93, 252
AclWI GGATC 1 cut(s) 914
AcsI RAATTY 3 cut(s) 192, 581, 908
AcuI CTGAAG 1 cut(s) 297
AcyI GRCGYC 2 cut(s) 90, 839
AfaI GTAC 3 cut(s) 598, 602, 1175
AfiI CCNNNNNNNGG 6 cut(s) 12, 178, 179, 278, 433, 518
AflIII ACRYGT 1 cut(s) 1249
AgsI TTSAA 9 cut(s) 226, 292, 748, 794, 832, 1021, 1048, 1168, 1186
AjnI CCWGG 2 cut(s) 426, 703
AluBI AGCT 8 cut(s) 106, 152, 286, 422, 1085, 1093, 1319, 1426
AluI AGCT 8 cut(s) 106, 152, 286, 422, 1085, 1093, 1319, 1426
Alw21I GWGCWC 1 cut(s) 108
AlwI GGATC 1 cut(s) 914
AlwNI CAGNNNCTG 2 cut(s) 1286, 1376
AoxI GGCC 1 cut(s) 520
ApeKI GCWGC 4 cut(s) 149, 976, 1085, 1367
ApoI RAATTY 3 cut(s) 192, 581, 908
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
Asp700I GAANNNNTTC 2 cut(s) 237, 411
AsuC2I CCSGG 2 cut(s) 13, 896
BanI GGYRCC 2 cut(s) 136, 1331
BanII GRGCYC 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 619
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 4 cut(s) 161, 988, 1072, 1379
BccI CCATC 2 cut(s) 427, 457
BciT130I CCWGG 2 cut(s) 428, 705
BcnI CCSGG 2 cut(s) 13, 896
BfaI CTAG 4 cut(s) 81, 552, 716, 971
BfuAI ACCTGC 1 cut(s) 612
BglII AGATCT 1 cut(s) 825
BisI GCNGC 4 cut(s) 150, 977, 1086, 1368
BlpI GCTNAGC 1 cut(s) 1089
BlsI GCNGC 4 cut(s) 151, 978, 1087, 1369
Bme1390I CCNGG 4 cut(s) 13, 428, 705, 896
BmiI GGNNCC 2 cut(s) 138, 1333
BmrFI CCNGG 4 cut(s) 13, 428, 705, 896
BmsI GCATC 4 cut(s) 277, 777, 1024, 1143
BpiI GAAGAC 1 cut(s) 619
Bpu1102I GCTNAGC 1 cut(s) 1089
BpuEI CTTGAG 2 cut(s) 402, 599
BpuMI CCSGG 2 cut(s) 13, 896
BsaAI YACGTR 1 cut(s) 600
BsaHI GRCGYC 2 cut(s) 90, 839
BsaJI CCNNGG 1 cut(s) 427
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 178, 179, 278, 433, 518
Bse1I ACTGG 2 cut(s) 395, 726
BseBI CCWGG 2 cut(s) 428, 705
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 7 cut(s) 268, 438, 442, 468, 888, 1134, 1361
BseLI CCNNNNNNNGG 6 cut(s) 12, 178, 179, 278, 433, 518
BseMII CTCAG 3 cut(s) 892, 987, 1080
BseNI ACTGG 2 cut(s) 395, 726
BseRI GAGGAG 4 cut(s) 43, 116, 140, 143
BseXI GCAGC 4 cut(s) 161, 988, 1072, 1379
BsgI GTGCAG 3 cut(s) 516, 839, 995
BshFI GGCC 1 cut(s) 522
BshNI GGYRCC 2 cut(s) 136, 1331
BsiHKAI GWGCWC 1 cut(s) 108
BsiSI CCGG 2 cut(s) 13, 896
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 6 cut(s) 12, 178, 179, 278, 433, 518
BsmFI GGGAC 1 cut(s) 98
BsmI GAATGC 1 cut(s) 683
BsnI GGCC 1 cut(s) 522
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 3 cut(s) 610, 825, 919
Bsp1720I GCTNAGC 1 cut(s) 1089
BspANI GGCC 1 cut(s) 522
BspCNI CTCAG 3 cut(s) 891, 988, 1081
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 2 cut(s) 138, 1333
BspMI ACCTGC 1 cut(s) 612
BspPI GGATC 1 cut(s) 914
BspT107I GGYRCC 2 cut(s) 136, 1331
BsrI ACTGG 2 cut(s) 395, 726
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 3 cut(s) 610, 825, 919
BssNI GRCGYC 2 cut(s) 90, 839
Bst2UI CCWGG 2 cut(s) 428, 705
Bst4CI ACNGT 1 cut(s) 200
Bst6I CTCTTC 1 cut(s) 117
BstACI GRCGYC 2 cut(s) 90, 839
BstBAI YACGTR 1 cut(s) 600
BstC8I GCNNGC 3 cut(s) 284, 520, 683
BstDEI CTNAG 5 cut(s) 295, 364, 878, 996, 1089
BstENI CCTNNNNNAGG 2 cut(s) 276, 516
BstF5I GGATG 7 cut(s) 268, 438, 442, 468, 888, 1134, 1361
BstKTI GATC 3 cut(s) 613, 828, 922
BstMBI GATC 3 cut(s) 610, 825, 919
BstMWI GCNNNNNNNGC 1 cut(s) 900
BstNI CCWGG 2 cut(s) 428, 705
BstNSI RCATGY 3 cut(s) 808, 1279, 1459
BstSCI CCNGG 4 cut(s) 11, 426, 703, 894
BstSNI TACGTA 1 cut(s) 600
BstV1I GCAGC 4 cut(s) 161, 988, 1072, 1379
BstV2I GAAGAC 1 cut(s) 619
BstX2I RGATCY 2 cut(s) 825, 919
BstYI RGATCY 2 cut(s) 825, 919
BsuRI GGCC 1 cut(s) 522
BtsCI GGATG 7 cut(s) 268, 438, 442, 468, 888, 1134, 1361
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 3 cut(s) 402, 719, 1443
BveI ACCTGC 1 cut(s) 612
Cac8I GCNNGC 3 cut(s) 284, 520, 683
CaiI CAGNNNCTG 2 cut(s) 1286, 1376
CciI TCATGA 1 cut(s) 577
CseI GACGC 1 cut(s) 847
Csp6I GTAC 3 cut(s) 597, 601, 1174
CviAII CATG 8 cut(s) 70, 335, 404, 578, 805, 965, 1276, 1456
CviQI GTAC 3 cut(s) 597, 601, 1174
DdeI CTNAG 5 cut(s) 295, 364, 878, 996, 1089
DpnI GATC 3 cut(s) 612, 827, 921
DpnII GATC 3 cut(s) 610, 825, 919
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
EciI GGCGGA 1 cut(s) 881
Ecl136II GAGCTC 1 cut(s) 106
Eco105I TACGTA 1 cut(s) 600
Eco24I GRGCYC 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 106
EcoNI CCTNNNNNAGG 2 cut(s) 276, 516
EcoRI GAATTC 1 cut(s) 192
EcoRII CCWGG 2 cut(s) 426, 703
EcoT22I ATGCAT 1 cut(s) 1039
EcoT38I GRGCYC 1 cut(s) 108
FaeI CATG 8 cut(s) 73, 338, 407, 581, 808, 968, 1279, 1459
FalI AAGNNNNNCTT 2 cut(s) 944, 976
FaqI GGGAC 1 cut(s) 98
FatI CATG 8 cut(s) 69, 334, 403, 577, 804, 964, 1275, 1455
FauI CCCGC 1 cut(s) 690
FblI GTMKAC 2 cut(s) 93, 252
Fnu4HI GCNGC 4 cut(s) 150, 977, 1086, 1368
FokI GGATG 7 cut(s) 255, 445, 449, 475, 875, 1121, 1348
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 4 cut(s) 150, 977, 1086, 1368
FspBI CTAG 4 cut(s) 81, 552, 716, 971
GluI GCNGC 4 cut(s) 150, 977, 1086, 1368
HaeIII GGCC 1 cut(s) 522
HapII CCGG 2 cut(s) 13, 896
HgaI GACGC 1 cut(s) 847
Hin1I GRCGYC 2 cut(s) 90, 839
Hin1II CATG 8 cut(s) 73, 338, 407, 581, 808, 968, 1279, 1459
HincII GTYRAC 5 cut(s) 160, 253, 957, 993, 1255
HindII GTYRAC 5 cut(s) 160, 253, 957, 993, 1255
HinfI GANTC 7 cut(s) 143, 254, 794, 1182, 1204, 1283, 1373
HpaI GTTAAC 2 cut(s) 993, 1255
HpaII CCGG 2 cut(s) 13, 896
Hpy166II GTNNAC 6 cut(s) 94, 160, 253, 957, 993, 1255
Hpy188I TCNGA 6 cut(s) 212, 277, 698, 1111, 1197, 1378
Hpy8I GTNNAC 6 cut(s) 94, 160, 253, 957, 993, 1255
Hpy99I CGWCG 4 cut(s) 65, 92, 101, 113
HpyAV CCTTC 3 cut(s) 127, 272, 282
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4IV ACGT 5 cut(s) 90, 599, 753, 1249, 1352
HpyF10VI GCNNNNNNNGC 1 cut(s) 900
HpyF3I CTNAG 5 cut(s) 295, 364, 878, 996, 1089
HpySE526I ACGT 5 cut(s) 90, 599, 753, 1249, 1352
Hsp92I GRCGYC 2 cut(s) 90, 839
Hsp92II CATG 8 cut(s) 73, 338, 407, 581, 808, 968, 1279, 1459
KspAI GTTAAC 2 cut(s) 993, 1255
Kzo9I GATC 3 cut(s) 610, 825, 919
LmnI GCTCC 2 cut(s) 103, 1423
Lsp1109I GCAGC 4 cut(s) 161, 988, 1072, 1379
LweI GCATC 4 cut(s) 277, 777, 1024, 1143
MaeI CTAG 4 cut(s) 81, 552, 716, 971
MaeII ACGT 5 cut(s) 90, 599, 753, 1249, 1352
MaeIII GTNAC 4 cut(s) 15, 779, 1101, 1115
MalI GATC 3 cut(s) 612, 827, 921
MboI GATC 3 cut(s) 610, 825, 919
MboII GAAGA 9 cut(s) 131, 134, 230, 619, 720, 820, 1018, 1054, 1169
MflI RGATCY 2 cut(s) 825, 919
MhlI GDGCHC 1 cut(s) 108
MluCI AATT 7 cut(s) 192, 372, 557, 581, 908, 1236, 1347
MlyI GAGTC 3 cut(s) 137, 248, 1367
MmeI TCCRAC 2 cut(s) 169, 676
Mph1103I ATGCAT 1 cut(s) 1039
MroXI GAANNNNTTC 2 cut(s) 237, 411
MseI TTAA 3 cut(s) 992, 1254, 1465
MslI CAYNNNNRTG 1 cut(s) 1454
MspI CCGG 2 cut(s) 13, 896
MspR9I CCNGG 4 cut(s) 13, 428, 705, 896
Mva1269I GAATGC 1 cut(s) 683
MvaI CCWGG 2 cut(s) 428, 705
MwoI GCNNNNNNNGC 1 cut(s) 900
NciI CCSGG 2 cut(s) 13, 896
NdeII GATC 3 cut(s) 610, 825, 919
NlaIII CATG 8 cut(s) 73, 338, 407, 581, 808, 968, 1279, 1459
NlaIV GGNNCC 2 cut(s) 138, 1333
NmuCI GTSAC 1 cut(s) 779
NsiI ATGCAT 1 cut(s) 1039
NspI RCATGY 3 cut(s) 808, 1279, 1459
PagI TCATGA 1 cut(s) 577
PcsI WCGNNNNNNNCGW 5 cut(s) 93, 96, 105, 114, 1071
PctI GAATGC 1 cut(s) 683
PdmI GAANNNNTTC 2 cut(s) 237, 411
PfeI GAWTC 4 cut(s) 794, 1182, 1204, 1283
PflFI GACNNNGTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 703
PkrI GCNGC 4 cut(s) 151, 978, 1087, 1369
PleI GAGTC 3 cut(s) 137, 248, 1367
PpsI GAGTC 3 cut(s) 137, 248, 1367
Ppu21I YACGTR 1 cut(s) 600
Psp124BI GAGCTC 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 426, 703
PspGI CCWGG 2 cut(s) 426, 703
PspN4I GGNNCC 2 cut(s) 138, 1333
PstNI CAGNNNCTG 2 cut(s) 1286, 1376
PsuI RGATCY 2 cut(s) 825, 919
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 3 cut(s) 598, 602, 1175
RsaNI GTAC 3 cut(s) 597, 601, 1174
RseI CAYNNNNRTG 1 cut(s) 1454
SacI GAGCTC 1 cut(s) 108
SalI GTCGAC 1 cut(s) 251
SaqAI TTAA 3 cut(s) 992, 1254, 1465
SatI GCNGC 4 cut(s) 150, 977, 1086, 1368
Sau3AI GATC 3 cut(s) 610, 825, 919
SchI GAGTC 3 cut(s) 137, 248, 1367
ScrFI CCNGG 4 cut(s) 13, 428, 705, 896
SduI GDGCHC 1 cut(s) 108
SfaNI GCATC 4 cut(s) 277, 777, 1024, 1143
SmiMI CAYNNNNRTG 1 cut(s) 1454
SmlI CTYRAG 2 cut(s) 417, 614
SmoI CTYRAG 2 cut(s) 417, 614
SnaBI TACGTA 1 cut(s) 600
Sse9I AATT 7 cut(s) 192, 372, 557, 581, 908, 1236, 1347
SspMI CTAG 4 cut(s) 81, 552, 716, 971
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 4 cut(s) 11, 426, 703, 894
TaaI ACNGT 1 cut(s) 200
TaiI ACGT 5 cut(s) 93, 602, 756, 1252, 1355
TaqI TCGA 4 cut(s) 63, 111, 252, 1245
TasI AATT 7 cut(s) 192, 372, 557, 581, 908, 1236, 1347
TfiI GAWTC 4 cut(s) 794, 1182, 1204, 1283
Tru1I TTAA 3 cut(s) 992, 1254, 1465
Tru9I TTAA 3 cut(s) 992, 1254, 1465
TscAI CASTG 3 cut(s) 402, 726, 1450
TseFI GTSAC 1 cut(s) 779
TseI GCWGC 4 cut(s) 149, 976, 1085, 1367
Tsp45I GTSAC 1 cut(s) 779
TspDTI ATGAA 6 cut(s) 420, 594, 789, 960, 1163, 1350
TspGWI ACGGA 2 cut(s) 177, 343
TspRI CASTG 3 cut(s) 402, 726, 1450
Tth111I GACNNNGTC 1 cut(s) 89
XagI CCTNNNNNAGG 2 cut(s) 276, 516
XapI RAATTY 3 cut(s) 192, 581, 908
XceI RCATGY 3 cut(s) 808, 1279, 1459
XmiI GTMKAC 2 cut(s) 93, 252
XmnI GAANNNNTTC 2 cut(s) 237, 411
XspI CTAG 4 cut(s) 81, 552, 716, 971
ZraI GACGTC 1 cut(s) 91
Zsp2I ATGCAT 1 cut(s) 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.