FvH4_6g29970

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001445
Physical Location & Seq
Forward (+)
23172218 .. 23178175
5958 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29970.t2

Sequence Viewer

Length: 1287 bp
ATGGGTGAAATCAGAGACAACGATGGGTACGAGGAAGAGCTCGTCGACTACGACGAGGAAGAGCAAAACGCCCCCAACTCCGTCTCCGGCAAACCCAATGGCGAATCCGTGAAAAAGGGCTATGTCGGCATCCATAGTTCGGGATTCAGAGACTTCCTGTTGAAACCAGAGCTGCTTCGAGCGATTGTGGATTCTGGATTCGAACATCCATCTGAAGTTCAACACGAGTGCATCCCTCAAGCTATTTTAGGAATGGATGTTCTTTGCCAAGCGAAATCTGGGATGGGAAAAACTGCAGTCTTTGTTCTGTCAACTCTTCAGCAAATAGAACCGGTTGCTGGTCAAGTTGCTGCTCTCATTCTTTGCCACACAAGAGAATTGGCTTACCAGATTTGTCATGAATTTGAGAGGTTTAGTACTTACTTGCCGGAGCTAAAAGTTGCCGTCTTCTATGGAGGTGTAAGTATTAAGGTTCACAGAGATTTGCTGAAAAATGAATGCCCTCATATTGTTGTGGGAACACCTGGGAGAGTGTTGGCTCTGGCGAGAGATAAAGAGCTTGGACTGAAGAATGTGAGGCATTTTATTCTTGATGAATGCGACAAGATGCTTGAATCACTTGATATGAGGAGAGATGTGCAGATGATCTTCAAGTTGACTCCTCATGACAAGCAAGTGATGATGTTTTCTGCAACACTAAGCAAGGAGATCCGCCCTGTCTGCAAGAAATTTATGCAAGATCCGATGGAGATTTATGTTGATGATGAAGCGAAGTTGACGCTTCATGGTCTCGTGCAGCATTACATCAAATTGAGCGAATCTGAGAAGAACCGCAAGCTGACCGACCTTCTAGATGCATTGGATTTCAATCAAGTTGTTATTTTTGTAAAAAGTGTGAGCAGAGCAGCTGAGTTGAACAAGTTACTTGCTGACTGTAATTTCCCCTCTATCTGCATTCATTCTGGAATGCCACAGGAAGAAAGGTTGAAAAGGTACAAGAATTTCAAGGAGGGCCTTTCAAGGATACTTGTGGCAACAGACTTAGTAGGAAGAGGAATTGACATTGAACGTGTTAACATTGTTATCAACTATGACATGCCAGATTCTGCTGATACCTACCTACACAGGGTTGGTAGAGCCGGAAGGTTTGGTACTAAAGGGTTGGCAATAACATTTGTCTCATCCTCAGCTGACTCTGATGTACTTAATCAGGTCCAGTCAAGGTTTGAAGTGGACATAAAGGAGCTCCCAGAGCAAATTGATACTTCTACATACATGCAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006974 GO:0007275 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009555 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:1901360 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

429

Amino Acids

48.28

Weight (kDa)

5.54

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 57 - 222 5.8e-40 DEAD/DEAH box helicase
ResIII PF04851 72 - 218 2.8e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 261 - 369 9.2e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 45
AciI CCGC 2 cut(s) 712, 832
AclWI GGATC 2 cut(s) 703, 734
AcsI RAATTY 3 cut(s) 401, 728, 1000
AcuI CTGAAG 3 cut(s) 234, 302, 587
AfaI GTAC 5 cut(s) 29, 418, 995, 1153, 1203
AfiI CCNNNNNNNGG 3 cut(s) 139, 338, 1126
AflIII ACRYGT 1 cut(s) 1069
AgeI ACCGGT 1 cut(s) 331
AjnI CCWGG 1 cut(s) 523
AloI GAACNNNNNNTCC 2 cut(s) 243, 275
AluBI AGCT 9 cut(s) 40, 172, 242, 433, 559, 838, 908, 1190, 1246
AluI AGCT 9 cut(s) 40, 172, 242, 433, 559, 838, 908, 1190, 1246
Alw21I GWGCWC 2 cut(s) 42, 1248
Alw26I GTCTC 5 cut(s) 9, 88, 144, 794, 1183
AlwI GGATC 2 cut(s) 703, 734
AlwNI CAGNNNCTG 1 cut(s) 1106
AoxI GGCC 1 cut(s) 1012
ApeKI GCWGC 4 cut(s) 172, 350, 796, 905
ApoI RAATTY 3 cut(s) 401, 728, 1000
AsiGI ACCGGT 1 cut(s) 331
AspS9I GGNCC 2 cut(s) 1012, 1213
AsuHPI GGTGA 1 cut(s) 17
AsuII TTCGAA 1 cut(s) 201
AvaII GGWCC 1 cut(s) 1213
BanII GRGCYC 2 cut(s) 42, 1248
BarI GAAGNNNNNNTAC 2 cut(s) 1135, 1167
BauI CACGAG 2 cut(s) 224, 791
BbsI GAAGAC 1 cut(s) 439
Bbv12I GWGCWC 2 cut(s) 42, 1248
BbvCI CCTCAGC 1 cut(s) 1186
BbvI GCAGC 4 cut(s) 159, 337, 808, 917
BccI CCATC 4 cut(s) 17, 217, 277, 739
BceAI ACGGC 1 cut(s) 428
BciT130I CCWGG 1 cut(s) 525
BciVI GTATCC 1 cut(s) 1017
BcoDI GTCTC 5 cut(s) 9, 88, 144, 794, 1183
BfaI CTAG 1 cut(s) 851
BfmI CTRYAG 1 cut(s) 294
BfuI GTATCC 1 cut(s) 1017
BisI GCNGC 4 cut(s) 173, 351, 797, 906
BlsI GCNGC 4 cut(s) 174, 352, 798, 907
BmcAI AGTACT 1 cut(s) 418
Bme1390I CCNGG 1 cut(s) 525
Bme18I GGWCC 1 cut(s) 1213
BmgT120I GGNCC 2 cut(s) 1012, 1213
BmrFI CCNGG 1 cut(s) 525
BmsI GCATC 4 cut(s) 138, 240, 597, 844
BpiI GAAGAC 1 cut(s) 439
Bpu10I CCTNAGC 1 cut(s) 1186
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 222
BsaBI GATNNNNATC 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 794
BsaJI CCNNGG 1 cut(s) 524
BsaWI WCCGGW 1 cut(s) 331
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 338, 1126
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 1 cut(s) 1216
Bse8I GATNNNNATC 1 cut(s) 867
BseBI CCWGG 1 cut(s) 525
BseDI CCNNGG 1 cut(s) 524
BseGI GGATG 6 cut(s) 129, 205, 231, 262, 288, 1181
BseJI GATNNNNATC 1 cut(s) 867
BseLI CCNNNNNNNGG 3 cut(s) 139, 338, 1126
BseMII CTCAG 3 cut(s) 813, 900, 1200
BseNI ACTGG 1 cut(s) 1216
BseRI GAGGAG 2 cut(s) 643, 651
BseXI GCAGC 4 cut(s) 159, 337, 808, 917
BsgI GTGCAG 2 cut(s) 659, 815
BshFI GGCC 1 cut(s) 1014
BshTI ACCGGT 1 cut(s) 331
BsiHKAI GWGCWC 2 cut(s) 42, 1248
BsiSI CCGG 4 cut(s) 87, 332, 428, 1140
BslI CCNNNNNNNGG 3 cut(s) 139, 338, 1126
BsmAI GTCTC 5 cut(s) 9, 88, 144, 794, 1183
BsmBI CGTCTC 1 cut(s) 88
BsmI GAATGC 4 cut(s) 503, 602, 954, 972
BsnI GGCC 1 cut(s) 1014
Bso31I GGTCTC 1 cut(s) 794
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 2 cut(s) 42, 1248
Bsp143I GATC 3 cut(s) 645, 708, 739
BspACI CCGC 2 cut(s) 712, 832
BspANI GGCC 1 cut(s) 1014
BspCNI CTCAG 3 cut(s) 814, 901, 1199
BspHI TCATGA 2 cut(s) 397, 664
BspMAI CTGCAG 1 cut(s) 298
BspPI GGATC 2 cut(s) 703, 734
BspQI GCTCTTC 2 cut(s) 30, 54
BspT104I TTCGAA 1 cut(s) 201
BspTNI GGTCTC 1 cut(s) 794
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 1 cut(s) 1216
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 1 cut(s) 524
BssMI GATC 3 cut(s) 645, 708, 739
BssSI CACGAG 2 cut(s) 224, 791
Bst2BI CACGAG 2 cut(s) 224, 791
Bst2UI CCWGG 1 cut(s) 525
Bst4CI ACNGT 1 cut(s) 935
Bst6I CTCTTC 4 cut(s) 30, 54, 321, 1045
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 836
BstDEI CTNAG 5 cut(s) 698, 822, 909, 1042, 1186
BstF5I GGATG 6 cut(s) 129, 205, 231, 262, 288, 1181
BstKTI GATC 3 cut(s) 648, 711, 742
BstMAI GTCTC 5 cut(s) 9, 88, 144, 794, 1183
BstMBI GATC 3 cut(s) 645, 708, 739
BstMWI GCNNNNNNNGC 3 cut(s) 126, 720, 1252
BstNI CCWGG 1 cut(s) 525
BstNSI RCATGY 2 cut(s) 1099, 1279
BstSCI CCNGG 1 cut(s) 523
BstSFI CTRYAG 1 cut(s) 294
BstV1I GCAGC 4 cut(s) 159, 337, 808, 917
BstV2I GAAGAC 1 cut(s) 439
BstX2I RGATCY 2 cut(s) 708, 739
BstYI RGATCY 2 cut(s) 708, 739
BsuI GTATCC 1 cut(s) 1017
BsuRI GGCC 1 cut(s) 1014
BtsCI GGATG 6 cut(s) 129, 205, 231, 262, 288, 1181
Cac8I GCNNGC 1 cut(s) 836
CaiI CAGNNNCTG 1 cut(s) 1106
CciI TCATGA 2 cut(s) 397, 664
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 1012, 1213
CseI GACGC 1 cut(s) 787
Csp6I GTAC 5 cut(s) 28, 417, 994, 1152, 1202
CspAI ACCGGT 1 cut(s) 331
CviAII CATG 5 cut(s) 398, 665, 785, 1096, 1276
CviQI GTAC 5 cut(s) 28, 417, 994, 1152, 1202
DdeI CTNAG 5 cut(s) 698, 822, 909, 1042, 1186
DpnI GATC 3 cut(s) 647, 710, 741
DpnII GATC 3 cut(s) 645, 708, 739
Eam1104I CTCTTC 4 cut(s) 30, 54, 321, 1045
EarI CTCTTC 4 cut(s) 30, 54, 321, 1045
EciI GGCGGA 1 cut(s) 701
Ecl136II GAGCTC 2 cut(s) 40, 1246
Eco24I GRGCYC 2 cut(s) 42, 1248
Eco31I GGTCTC 1 cut(s) 794
Eco47I GGWCC 1 cut(s) 1213
Eco53kI GAGCTC 2 cut(s) 40, 1246
Eco57I CTGAAG 3 cut(s) 234, 302, 587
EcoICRI GAGCTC 2 cut(s) 40, 1246
EcoO109I RGGNCCY 1 cut(s) 1012
EcoRII CCWGG 1 cut(s) 523
EcoT22I ATGCAT 1 cut(s) 859
EcoT38I GRGCYC 2 cut(s) 42, 1248
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 5 cut(s) 401, 668, 788, 1099, 1279
FatI CATG 5 cut(s) 397, 664, 784, 1095, 1275
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 4 cut(s) 173, 351, 797, 906
FokI GGATG 6 cut(s) 116, 192, 218, 269, 295, 1168
FriOI GRGCYC 2 cut(s) 42, 1248
Fsp4HI GCNGC 4 cut(s) 173, 351, 797, 906
FspBI CTAG 1 cut(s) 851
GluI GCNGC 4 cut(s) 173, 351, 797, 906
HaeIII GGCC 1 cut(s) 1014
HapII CCGG 4 cut(s) 87, 332, 428, 1140
HgaI GACGC 1 cut(s) 787
Hin1II CATG 5 cut(s) 401, 668, 788, 1099, 1279
HincII GTYRAC 5 cut(s) 46, 312, 657, 777, 1075
HindII GTYRAC 5 cut(s) 46, 312, 657, 777, 1075
HinfI GANTC 9 cut(s) 104, 144, 191, 198, 614, 658, 818, 1103, 1193
HpaI GTTAAC 1 cut(s) 1075
HpaII CCGG 4 cut(s) 87, 332, 428, 1140
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 7 cut(s) 46, 312, 475, 657, 777, 1075, 1234
Hpy188I TCNGA 6 cut(s) 14, 149, 214, 744, 823, 1198
Hpy188III TCNNGA 7 cut(s) 141, 195, 398, 590, 665, 851, 963
Hpy8I GTNNAC 7 cut(s) 46, 312, 475, 657, 777, 1075, 1234
Hpy99I CGWCG 2 cut(s) 47, 56
HpyAV CCTTC 2 cut(s) 857, 1137
HpyCH4III ACNGT 1 cut(s) 935
HpyCH4IV ACGT 1 cut(s) 1069
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 720, 1252
HpyF3I CTNAG 5 cut(s) 698, 822, 909, 1042, 1186
HpySE526I ACGT 1 cut(s) 1069
Hsp92II CATG 5 cut(s) 401, 668, 788, 1099, 1279
KspAI GTTAAC 1 cut(s) 1075
Kzo9I GATC 3 cut(s) 645, 708, 739
LguI GCTCTTC 2 cut(s) 30, 54
LmnI GCTCC 3 cut(s) 430, 1243, 1251
Lsp1109I GCAGC 4 cut(s) 159, 337, 808, 917
LweI GCATC 4 cut(s) 138, 240, 597, 844
MaeI CTAG 1 cut(s) 851
MaeII ACGT 1 cut(s) 1069
MaeIII GTNAC 1 cut(s) 921
MalI GATC 3 cut(s) 647, 710, 741
MboI GATC 3 cut(s) 645, 708, 739
MboII GAAGA 9 cut(s) 47, 71, 308, 439, 580, 640, 838, 989, 1062
MflI RGATCY 2 cut(s) 708, 739
MhlI GDGCHC 2 cut(s) 42, 1248
MluCI AATT 8 cut(s) 377, 401, 728, 809, 937, 1000, 1056, 1257
MlyI GAGTC 2 cut(s) 652, 1187
Mph1103I ATGCAT 1 cut(s) 859
MseI TTAA 3 cut(s) 468, 1074, 1206
MspA1I CMGCKG 2 cut(s) 908, 1190
MspI CCGG 4 cut(s) 87, 332, 428, 1140
MspR9I CCNGG 1 cut(s) 525
Mva1269I GAATGC 4 cut(s) 503, 602, 954, 972
MvaI CCWGG 1 cut(s) 525
MwoI GCNNNNNNNGC 3 cut(s) 126, 720, 1252
NdeII GATC 3 cut(s) 645, 708, 739
NlaIII CATG 5 cut(s) 401, 668, 788, 1099, 1279
NsiI ATGCAT 1 cut(s) 859
NspI RCATGY 2 cut(s) 1099, 1279
NspV TTCGAA 1 cut(s) 201
PagI TCATGA 2 cut(s) 397, 664
PciSI GCTCTTC 2 cut(s) 30, 54
PcsI WCGNNNNNNNCGW 3 cut(s) 27, 48, 51
PctI GAATGC 4 cut(s) 503, 602, 954, 972
PfeI GAWTC 7 cut(s) 104, 144, 191, 198, 614, 818, 1103
PinAI ACCGGT 1 cut(s) 331
PkrI GCNGC 4 cut(s) 174, 352, 798, 907
PleI GAGTC 2 cut(s) 652, 1187
PpsI GAGTC 2 cut(s) 652, 1187
Psp124BI GAGCTC 2 cut(s) 42, 1248
Psp6I CCWGG 1 cut(s) 523
PspGI CCWGG 1 cut(s) 523
PspPI GGNCC 2 cut(s) 1012, 1213
PstI CTGCAG 1 cut(s) 298
PstNI CAGNNNCTG 1 cut(s) 1106
PsuI RGATCY 2 cut(s) 708, 739
PvuII CAGCTG 2 cut(s) 908, 1190
RsaI GTAC 5 cut(s) 29, 418, 995, 1153, 1203
RsaNI GTAC 5 cut(s) 28, 417, 994, 1152, 1202
SacI GAGCTC 2 cut(s) 42, 1248
SalI GTCGAC 1 cut(s) 44
SapI GCTCTTC 2 cut(s) 30, 54
SaqAI TTAA 3 cut(s) 468, 1074, 1206
SatI GCNGC 4 cut(s) 173, 351, 797, 906
Sau3AI GATC 3 cut(s) 645, 708, 739
Sau96I GGNCC 2 cut(s) 1012, 1213
ScaI AGTACT 1 cut(s) 418
SchI GAGTC 2 cut(s) 652, 1187
ScrFI CCNGG 1 cut(s) 525
SduI GDGCHC 2 cut(s) 42, 1248
SfaNI GCATC 4 cut(s) 138, 240, 597, 844
SfcI CTRYAG 1 cut(s) 294
SfuI TTCGAA 1 cut(s) 201
SinI GGWCC 1 cut(s) 1213
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
Sse9I AATT 8 cut(s) 377, 401, 728, 809, 937, 1000, 1056, 1257
SsiI CCGC 2 cut(s) 712, 832
SspMI CTAG 1 cut(s) 851
SstI GAGCTC 2 cut(s) 42, 1248
StyD4I CCNGG 1 cut(s) 523
TaaI ACNGT 1 cut(s) 935
TaiI ACGT 1 cut(s) 1072
TaqI TCGA 3 cut(s) 45, 178, 201
TaqII GACCGA 1 cut(s) 857
TasI AATT 8 cut(s) 377, 401, 728, 809, 937, 1000, 1056, 1257
TatI WGTACW 2 cut(s) 416, 1201
TfiI GAWTC 7 cut(s) 104, 144, 191, 198, 614, 818, 1103
Tru1I TTAA 3 cut(s) 468, 1074, 1206
Tru9I TTAA 3 cut(s) 468, 1074, 1206
TseI GCWGC 4 cut(s) 172, 350, 796, 905
TspDTI ATGAA 6 cut(s) 414, 510, 609, 773, 780, 947
TspGWI ACGGA 2 cut(s) 70, 97
VpaK11BI GGWCC 1 cut(s) 1213
XapI RAATTY 3 cut(s) 401, 728, 1000
XbaI TCTAGA 1 cut(s) 850
XceI RCATGY 2 cut(s) 1099, 1279
XcmI CCANNNNNNNNNTGG 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 1 cut(s) 851
ZrmI AGTACT 1 cut(s) 418
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.