pycom08g12530

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001445
Physical Location & Seq
Reverse (-)
11003062 .. 11007962
4901 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g12530.6

Sequence Viewer

Length: 1506 bp
ATGAAGATATTTTTTACAAGCAATTTTTTACGTATATATATCCGCCTCCCAGTTGCCGTAACCCTATCTCTCAGACTCTCTCCTGCTCTCCTCTTTCGTCGAGGTGTCGTCCATCTCTCGTCTCTCTCTAACTACAAAAATTTCAAAATTCGCGTTTTCATCAAACTTTTTCAGTTGCAGAATCTTCAAAAATCCATACTTTCACTCTTAATTCGACCTCAGATCATGGGCGAAACTAGGGACGACGTCTACGACGAGGAGCTCGTCGATTACGAAGAAGAGGAGGAGAAGGCCCCCGACTCCGCAGCTAAAGTCAACGGAGAAGCCCCCAAAAAGGGTTATGTTGGAATTCACAGTTCAGGGTTCAGAGATTTCCTTTTGAAGCCTGAGCTTCTTCGGGCTATTGTCGACTCTGGATTTGAGCATCCTTCTGAAGTGCAACATGAATGTATTCCTCAAGCTATCCTGGGGATGGATGTTATATGTCAGGCTAAATCTGGGATGGGAAAAACTGCTGTTTTTGTTCTATCAACTTTACAGCAGATTGACCCTGTTGCAGGCCAAGTATCTGCACTTGTGCTTTGCCATACTCGAGAATTAGCATACCAGATTTGTCATGAATTTGAGAGGTTCAGTACGTACCTGCCTGATCTCAAGGTTGCTGTCTTCTATGGTGGTGTCAGTATCAAACTTCACAAAGATTTACTGAAAAATGAATGCCCGCACATTGTTGTCGGAACTCCCGGACGAATACTAGCACTGGCAAGAGATAAAGACCTGTCGTTGAAAAACGTGAGGCATTTTATTCTTGATGAATGTGACAAGATGCTTGAATCACTTGACATGCGGAGAGATGTGCAGGAGATCGTCAAGATGACGCCTCACGATAAGCAAGTGATGATGTTTTCCGCAACACTTAGCAAAGACATCCGCCCGGTTTGCAAGAAATTTATGCAAGATCCTATGGAAATATATGTTGATGATGAAGCCAAGTTGACCTTGCATGGACTAGTGCAGCACTACATCAAGTTAACTGAGGCAGAGAAGAACCGCAAGTTGAATGACCTCCTTGATGCATTGGACTTCAATCAAGTTGTTATCTTCGTCAAAAGCGTTAGCAGAGCTGCCGAGCTGAACAAGTTACTTTCAGAGTGTAACTTTCCCTCCATATGCATCCATTCTGGAATGAACCAAGAAGAAAGGTTGAAGCGGTACAAAGGATTCAAGGAGGGTCAGAACAGGATTCTTGTGGCAACTGATTTAGTTGGTAGAGGAATTGACATCGAACGCGTTAACATTGTTATCAACTACGACATGCCAGATTCTGCTGACACTTATTTACACAGGGTTGGCAGAGCTGGTAGGTTTGGCACCAAAGGTCTTGCAATTACGTTTGTTTCATCCGCAGCAGACTCCGATGTTCTAAATGATGTTCAGGAAAGGTTTGAGGTTGATATAAAGGAGCTTCCTGAACAGATTGACACAGCCACATACATGCCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

502

Amino Acids

56.86

Weight (kDa)

6.3

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 249
Acc36I ACCTGC 1 cut(s) 651
AccB1I GGYRCC 1 cut(s) 1370
AccI GTMKAC 2 cut(s) 249, 408
AccII CGCG 2 cut(s) 153, 1290
AciI CCGC 9 cut(s) 43, 303, 722, 847, 909, 931, 1051, 1210, 1404
AclWI GGATC 1 cut(s) 953
AcsI RAATTY 5 cut(s) 139, 147, 348, 620, 947
AcuI CTGAAG 1 cut(s) 453
AcyI GRCGYC 2 cut(s) 246, 878
AfaI GTAC 3 cut(s) 637, 641, 1214
AfiI CCNNNNNNNGG 4 cut(s) 334, 335, 472, 557
AflIII ACRYGT 1 cut(s) 1288
AgsI TTSAA 9 cut(s) 145, 188, 382, 787, 833, 1060, 1087, 1207, 1225
AhlI ACTAGT 1 cut(s) 1009
AjnI CCWGG 1 cut(s) 465
AluBI AGCT 8 cut(s) 262, 308, 391, 461, 1124, 1132, 1358, 1465
AluI AGCT 8 cut(s) 262, 308, 391, 461, 1124, 1132, 1358, 1465
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 1 cut(s) 953
AlwNI CAGNNNCTG 1 cut(s) 1325
Ama87I CYCGRG 1 cut(s) 591
AoxI GGCC 2 cut(s) 291, 559
ApeKI GCWGC 4 cut(s) 305, 1015, 1124, 1406
ApoI RAATTY 5 cut(s) 139, 147, 348, 620, 947
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
Asp700I GAANNNNTTC 1 cut(s) 450
AspS9I GGNCC 1 cut(s) 292
AsuC2I CCSGG 2 cut(s) 744, 935
AvaI CYCGRG 1 cut(s) 591
BanI GGYRCC 1 cut(s) 1370
BanII GRGCYC 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 658
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 4 cut(s) 317, 1027, 1111, 1418
BccI CCATC 3 cut(s) 120, 466, 496
BceAI ACGGC 1 cut(s) 41
BciT130I CCWGG 1 cut(s) 467
BcnI CCSGG 2 cut(s) 744, 935
BcoDI GTCTC 1 cut(s) 126
BcuI ACTAGT 1 cut(s) 1009
BfaI CTAG 3 cut(s) 237, 755, 1010
BfuAI ACCTGC 1 cut(s) 651
BisI GCNGC 4 cut(s) 306, 1016, 1125, 1407
BlsI GCNGC 4 cut(s) 307, 1017, 1126, 1408
Bme1390I CCNGG 3 cut(s) 467, 744, 935
BmeT110I CYCGRG 1 cut(s) 591
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 2 cut(s) 294, 1372
BmrFI CCNGG 3 cut(s) 467, 744, 935
BmrI ACTGGG 1 cut(s) 44
BmsI GCATC 4 cut(s) 433, 816, 1063, 1182
BmuI ACTGGG 1 cut(s) 44
BpiI GAAGAC 1 cut(s) 658
Bpu10I CCTNAGC 1 cut(s) 387
BpuEI CTTGAG 2 cut(s) 441, 638
BpuMI CCSGG 2 cut(s) 744, 935
BsaAI YACGTR 2 cut(s) 32, 639
BsaHI GRCGYC 2 cut(s) 246, 878
BsaJI CCNNGG 1 cut(s) 466
BsaXI ACNNNNNCTCC 2 cut(s) 1148, 1178
Bsc4I CCNNNNNNNGG 4 cut(s) 334, 335, 472, 557
Bse1I ACTGG 2 cut(s) 50, 765
BseBI CCWGG 1 cut(s) 467
BseDI CCNNGG 1 cut(s) 466
BseGI GGATG 7 cut(s) 424, 477, 481, 507, 927, 1173, 1400
BseLI CCNNNNNNNGG 4 cut(s) 334, 335, 472, 557
BseMII CTCAG 4 cut(s) 85, 233, 378, 1026
BseNI ACTGG 2 cut(s) 50, 765
BseRI GAGGAG 4 cut(s) 80, 272, 296, 299
BseXI GCAGC 4 cut(s) 317, 1027, 1111, 1418
BsgI GTGCAG 3 cut(s) 555, 878, 1034
Bsh1236I CGCG 2 cut(s) 153, 1290
BshFI GGCC 2 cut(s) 293, 561
BshNI GGYRCC 1 cut(s) 1370
BsiHKAI GWGCWC 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 2 cut(s) 744, 935
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 4 cut(s) 334, 335, 472, 557
BsmAI GTCTC 1 cut(s) 126
BsmBI CGTCTC 1 cut(s) 126
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 722
BsnI GGCC 2 cut(s) 293, 561
BsoBI CYCGRG 1 cut(s) 591
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 4 cut(s) 222, 649, 864, 958
BspACI CCGC 9 cut(s) 43, 303, 722, 847, 909, 931, 1051, 1210, 1404
BspANI GGCC 2 cut(s) 293, 561
BspCNI CTCAG 4 cut(s) 84, 232, 379, 1027
BspFNI CGCG 2 cut(s) 153, 1290
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 2 cut(s) 294, 1372
BspMI ACCTGC 1 cut(s) 651
BspPI GGATC 1 cut(s) 953
BspT107I GGYRCC 1 cut(s) 1370
BsrI ACTGG 2 cut(s) 50, 765
BssECI CCNNGG 1 cut(s) 466
BssMI GATC 4 cut(s) 222, 649, 864, 958
BssNI GRCGYC 2 cut(s) 246, 878
Bst2UI CCWGG 1 cut(s) 467
Bst4CI ACNGT 1 cut(s) 356
Bst6I CTCTTC 1 cut(s) 273
BstACI GRCGYC 2 cut(s) 246, 878
BstBAI YACGTR 2 cut(s) 32, 639
BstC8I GCNNGC 2 cut(s) 559, 722
BstDEI CTNAG 5 cut(s) 71, 219, 387, 917, 1035
BstENI CCTNNNNNAGG 1 cut(s) 555
BstF5I GGATG 7 cut(s) 424, 477, 481, 507, 927, 1173, 1400
BstFNI CGCG 2 cut(s) 153, 1290
BstKTI GATC 4 cut(s) 225, 652, 867, 961
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 4 cut(s) 222, 649, 864, 958
BstMWI GCNNNNNNNGC 1 cut(s) 939
BstNI CCWGG 1 cut(s) 467
BstNSI RCATGY 3 cut(s) 847, 1318, 1498
BstSCI CCNGG 3 cut(s) 465, 742, 933
BstSNI TACGTA 2 cut(s) 32, 639
BstUI CGCG 2 cut(s) 153, 1290
BstV1I GCAGC 4 cut(s) 317, 1027, 1111, 1418
BstV2I GAAGAC 1 cut(s) 658
BstX2I RGATCY 1 cut(s) 958
BstYI RGATCY 1 cut(s) 958
BsuRI GGCC 2 cut(s) 293, 561
BtsCI GGATG 7 cut(s) 424, 477, 481, 507, 927, 1173, 1400
BtsIMutI CAGTG 1 cut(s) 758
BveI ACCTGC 1 cut(s) 651
Cac8I GCNNGC 2 cut(s) 559, 722
CaiI CAGNNNCTG 1 cut(s) 1325
CciI TCATGA 1 cut(s) 616
Cfr13I GGNCC 1 cut(s) 292
CseI GACGC 1 cut(s) 886
Csp6I GTAC 3 cut(s) 636, 640, 1213
CviAII CATG 7 cut(s) 226, 443, 617, 844, 1004, 1315, 1495
CviQI GTAC 3 cut(s) 636, 640, 1213
DdeI CTNAG 5 cut(s) 71, 219, 387, 917, 1035
DpnI GATC 4 cut(s) 224, 651, 866, 960
DpnII GATC 4 cut(s) 222, 649, 864, 958
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
EciI GGCGGA 2 cut(s) 32, 920
Ecl136II GAGCTC 1 cut(s) 262
Eco105I TACGTA 2 cut(s) 32, 639
Eco24I GRGCYC 1 cut(s) 264
Eco53kI GAGCTC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 591
EcoICRI GAGCTC 1 cut(s) 262
EcoNI CCTNNNNNAGG 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 292
EcoRI GAATTC 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 465
EcoT22I ATGCAT 2 cut(s) 1078, 1175
EcoT38I GRGCYC 1 cut(s) 264
Esp3I CGTCTC 1 cut(s) 126
FaeI CATG 7 cut(s) 229, 446, 620, 847, 1007, 1318, 1498
FalI AAGNNNNNCTT 2 cut(s) 983, 1015
FaqI GGGAC 1 cut(s) 254
FatI CATG 7 cut(s) 225, 442, 616, 843, 1003, 1314, 1494
FauI CCCGC 1 cut(s) 729
FauNDI CATATG 1 cut(s) 1169
FblI GTMKAC 2 cut(s) 249, 408
Fnu4HI GCNGC 4 cut(s) 306, 1016, 1125, 1407
FokI GGATG 7 cut(s) 411, 484, 488, 514, 914, 1160, 1387
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 4 cut(s) 306, 1016, 1125, 1407
FspBI CTAG 3 cut(s) 237, 755, 1010
GluI GCNGC 4 cut(s) 306, 1016, 1125, 1407
HaeIII GGCC 2 cut(s) 293, 561
HapII CCGG 2 cut(s) 744, 935
HgaI GACGC 1 cut(s) 886
Hin1I GRCGYC 2 cut(s) 246, 878
Hin1II CATG 7 cut(s) 229, 446, 620, 847, 1007, 1318, 1498
HincII GTYRAC 5 cut(s) 316, 409, 996, 1032, 1294
HindII GTYRAC 5 cut(s) 316, 409, 996, 1032, 1294
HinfI GANTC 9 cut(s) 75, 181, 299, 410, 833, 1221, 1243, 1322, 1412
HpaI GTTAAC 2 cut(s) 1032, 1294
HpaII CCGG 2 cut(s) 744, 935
Hpy166II GTNNAC 6 cut(s) 250, 316, 409, 996, 1032, 1294
Hpy188I TCNGA 8 cut(s) 74, 222, 368, 433, 737, 1150, 1236, 1417
Hpy188III TCNNGA 9 cut(s) 414, 593, 617, 809, 871, 884, 1182, 1436, 1469
Hpy8I GTNNAC 6 cut(s) 250, 316, 409, 996, 1032, 1294
Hpy99I CGWCG 4 cut(s) 102, 248, 257, 269
HpyAV CCTTC 2 cut(s) 283, 438
HpyCH4III ACNGT 1 cut(s) 356
HpyCH4IV ACGT 5 cut(s) 31, 246, 638, 792, 1391
HpyF10VI GCNNNNNNNGC 1 cut(s) 939
HpyF3I CTNAG 5 cut(s) 71, 219, 387, 917, 1035
HpySE526I ACGT 5 cut(s) 31, 246, 638, 792, 1391
Hsp92I GRCGYC 2 cut(s) 246, 878
Hsp92II CATG 7 cut(s) 229, 446, 620, 847, 1007, 1318, 1498
KspAI GTTAAC 2 cut(s) 1032, 1294
Kzo9I GATC 4 cut(s) 222, 649, 864, 958
LmnI GCTCC 2 cut(s) 259, 1462
Lsp1109I GCAGC 4 cut(s) 317, 1027, 1111, 1418
LweI GCATC 4 cut(s) 433, 816, 1063, 1182
MaeI CTAG 3 cut(s) 237, 755, 1010
MaeII ACGT 5 cut(s) 31, 246, 638, 792, 1391
MaeIII GTNAC 4 cut(s) 58, 818, 1140, 1154
MalI GATC 4 cut(s) 224, 651, 866, 960
MboI GATC 4 cut(s) 222, 649, 864, 958
MboII GAAGA 9 cut(s) 16, 176, 287, 290, 386, 658, 1057, 1093, 1208
MflI RGATCY 1 cut(s) 958
MhlI GDGCHC 1 cut(s) 264
MluI ACGCGT 1 cut(s) 1288
MlyI GAGTC 4 cut(s) 69, 293, 404, 1406
MmeI TCCRAC 2 cut(s) 325, 715
Mph1103I ATGCAT 2 cut(s) 1078, 1175
MroXI GAANNNNTTC 1 cut(s) 450
MseI TTAA 4 cut(s) 209, 1031, 1293, 1504
MslI CAYNNNNRTG 1 cut(s) 1493
MspI CCGG 2 cut(s) 744, 935
MspR9I CCNGG 3 cut(s) 467, 744, 935
Mva1269I GAATGC 1 cut(s) 722
MvaI CCWGG 1 cut(s) 467
MvnI CGCG 2 cut(s) 153, 1290
MwoI GCNNNNNNNGC 1 cut(s) 939
NciI CCSGG 2 cut(s) 744, 935
NdeI CATATG 1 cut(s) 1169
NdeII GATC 4 cut(s) 222, 649, 864, 958
NlaIII CATG 7 cut(s) 229, 446, 620, 847, 1007, 1318, 1498
NlaIV GGNNCC 2 cut(s) 294, 1372
NmeAIII GCCGAG 1 cut(s) 1153
NmuCI GTSAC 1 cut(s) 818
NsiI ATGCAT 2 cut(s) 1078, 1175
NspI RCATGY 3 cut(s) 847, 1318, 1498
PaeR7I CTCGAG 1 cut(s) 591
PagI TCATGA 1 cut(s) 616
PcsI WCGNNNNNNNCGW 5 cut(s) 249, 252, 261, 270, 1110
PctI GAATGC 1 cut(s) 722
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 5 cut(s) 181, 833, 1221, 1243, 1322
PflFI GACNNNGTC 1 cut(s) 245
PfoI TCCNGGA 1 cut(s) 742
PkrI GCNGC 4 cut(s) 307, 1017, 1126, 1408
PleI GAGTC 4 cut(s) 69, 293, 404, 1406
PpsI GAGTC 4 cut(s) 69, 293, 404, 1406
Ppu21I YACGTR 2 cut(s) 32, 639
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 465
PspGI CCWGG 1 cut(s) 465
PspN4I GGNNCC 2 cut(s) 294, 1372
PspPI GGNCC 1 cut(s) 292
PstNI CAGNNNCTG 1 cut(s) 1325
PsuI RGATCY 1 cut(s) 958
PsyI GACNNNGTC 1 cut(s) 245
RsaI GTAC 3 cut(s) 637, 641, 1214
RsaNI GTAC 3 cut(s) 636, 640, 1213
RseI CAYNNNNRTG 1 cut(s) 1493
SacI GAGCTC 1 cut(s) 264
SalI GTCGAC 1 cut(s) 407
SaqAI TTAA 4 cut(s) 209, 1031, 1293, 1504
SatI GCNGC 4 cut(s) 306, 1016, 1125, 1407
Sau3AI GATC 4 cut(s) 222, 649, 864, 958
Sau96I GGNCC 1 cut(s) 292
SchI GAGTC 4 cut(s) 69, 293, 404, 1406
ScrFI CCNGG 3 cut(s) 467, 744, 935
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 4 cut(s) 433, 816, 1063, 1182
Sfr274I CTCGAG 1 cut(s) 591
SlaI CTCGAG 1 cut(s) 591
SmiMI CAYNNNNRTG 1 cut(s) 1493
SmlI CTYRAG 3 cut(s) 456, 591, 653
SmoI CTYRAG 3 cut(s) 456, 591, 653
SnaBI TACGTA 2 cut(s) 32, 639
SpeI ACTAGT 1 cut(s) 1009
SsiI CCGC 9 cut(s) 43, 303, 722, 847, 909, 931, 1051, 1210, 1404
SspMI CTAG 3 cut(s) 237, 755, 1010
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 3 cut(s) 465, 742, 933
TaaI ACNGT 1 cut(s) 356
TaiI ACGT 5 cut(s) 34, 249, 641, 795, 1394
TaqI TCGA 6 cut(s) 100, 214, 267, 408, 592, 1284
TfiI GAWTC 5 cut(s) 181, 833, 1221, 1243, 1322
Tru1I TTAA 4 cut(s) 209, 1031, 1293, 1504
Tru9I TTAA 4 cut(s) 209, 1031, 1293, 1504
TscAI CASTG 1 cut(s) 765
TseFI GTSAC 1 cut(s) 818
TseI GCWGC 4 cut(s) 305, 1015, 1124, 1406
Tsp45I GTSAC 1 cut(s) 818
TspDTI ATGAA 9 cut(s) 17, 148, 459, 633, 729, 828, 999, 1202, 1389
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 1 cut(s) 765
Tth111I GACNNNGTC 1 cut(s) 245
XagI CCTNNNNNAGG 1 cut(s) 555
XapI RAATTY 5 cut(s) 139, 147, 348, 620, 947
XceI RCATGY 3 cut(s) 847, 1318, 1498
XhoI CTCGAG 1 cut(s) 591
XmiI GTMKAC 2 cut(s) 249, 408
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 3 cut(s) 237, 755, 1010
ZraI GACGTC 1 cut(s) 247
Zsp2I ATGCAT 2 cut(s) 1078, 1175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.