Prupe.3G084000_v2.0.a1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001445
Physical Location & Seq
Reverse (-)
6205522 .. 6210618
5097 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G084000.2

Sequence Viewer

Length: 1305 bp
ATGGGTGAAATCAGAGACAACGATGGTTACGAAGAGGAGCTGGTCGATTATGAAGAGGAAGACCAAAATGCCCCCAACTCCGTCTCCGCCAAGCCCTCTGGTGATACCGTTAAAAAGGGCTATGTTGGCATCCACAGTTCAGGATTCAGAGACTTCCTGTTGAAACCAGAGCTTCTTCGAGCGATTGTGGATTCTGGGTTCGAACATCCTTCAGAAGTTCAACATGAGTGCATCCCTCAAGCTATCTTAGGAATGGATGTTATCTGTCAAGCAAAATCTGGGATGGGAAAGACCGCCGTCTTTGTTCTATCTACCCTTCAGCAAATAGATCCAATTGCTGGTCAGGTTGCTGCTCTTATTCTTTGCCACACAAGAGAACTGGCTTACCAGATTTGTCATGAATTTGAGAGATTCAGTACCTATTTGCCGGACCTGAAGGTTGCAGTCTTCTATGGAGGTGTCAGTATCAAGGTTCACAAAGACTTACTGAAAAATGAATGTCCTCACATTGTTGTTGGAACACCTGGGAGAATATTAGCTTTGGCAAGAGATAAAGAGCTTGGACTAAAGAATGTCAGACATTTTATTCTTGATGAATGTGATAAGATGCTTGAGTCACTTGATATGAGGAGAGATGTGCAGGAGATCTTTAAGTTGACTCCTCATGACAAGCAAGTGATGATGTTTTCAGCAACACTAAGCAAAGAAATCCGCCCTGTTTGCAAGAAATTTATGCAAGATCCCATGGAAATTTATGTAGATGATGAGGCCAAATTGACACTTCATGGTCTCGTTCAGCACTACATCAAATTGAGCGAACCTGAGAAAAACCGCAAGTTGACTGACCTTCTTGATGCATTGGATTTCAACCAAGTTGTTATTTTTGTAAAAAGTGTAAGCAGAGCAGCTGAGCTGAACAAATTACTTGCTGATTGTAACTTCCCATCTATCTGCATTCACTCTGGAATGCCTCAGGAAGAAAGGTTGAAACGTTACAAGAATTTCAAGGAGGGGCTTTCAAGGATTCTTGTGGCAACAGATTTAGTCGGAAGGGGAATTGATATCGAGCGTGTTAACATTGTTATTAATTATGACATGCCAGATTCTGCCGATACCTACTTACACAGGGTTGGCAGAGCTGGTAGGTTTGGTACCAAGGGACTGGCAATTACATTTGTCTCTTCGGCATCAGACTCTGATGTTCTTAATCAGGTCCAGTCAAGGTTTGAGGTGGACATAAAGGAGCTTCCGGAGCAAATTGATACTTCTACATACAGTAAGATAGCTTTTCTCACAAAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006974 GO:0007275 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009555 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:1901360 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

435

Amino Acids

48.88

Weight (kDa)

5.61

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1151
AccB1I GGYRCC 1 cut(s) 1151
AccB7I CCANNNNNTGG 1 cut(s) 338
AccIII TCCGGA 1 cut(s) 1249
AciI CCGC 4 cut(s) 87, 294, 712, 832
AclI AACGTT 1 cut(s) 991
AclWI GGATC 2 cut(s) 323, 734
AcsI RAATTY 4 cut(s) 401, 728, 750, 1000
AcuI CTGAAG 3 cut(s) 195, 302, 455
AfaI GTAC 2 cut(s) 418, 1153
AfiI CCNNNNNNNGG 1 cut(s) 338
AgsI TTSAA 6 cut(s) 163, 221, 868, 988, 1006, 1020
AjnI CCWGG 1 cut(s) 523
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
Alw26I GTCTC 5 cut(s) 9, 88, 144, 794, 1183
AlwI GGATC 2 cut(s) 323, 734
AlwNI CAGNNNCTG 2 cut(s) 1106, 1196
Aor13HI TCCGGA 1 cut(s) 1249
AoxI GGCC 1 cut(s) 768
ApeKI GCWGC 2 cut(s) 350, 905
ApoI RAATTY 4 cut(s) 401, 728, 750, 1000
AseI ATTAAT 1 cut(s) 1086
Asp718I GGTACC 1 cut(s) 1151
AspS9I GGNCC 2 cut(s) 430, 1213
AsuHPI GGTGA 2 cut(s) 17, 113
AsuII TTCGAA 1 cut(s) 201
AvaII GGWCC 2 cut(s) 430, 1213
AxyI CCTNAGG 1 cut(s) 972
BanI GGYRCC 1 cut(s) 1151
BbsI GAAGAC 2 cut(s) 66, 439
BbvI GCAGC 2 cut(s) 337, 917
BccI CCATC 3 cut(s) 17, 277, 952
BceAI ACGGC 1 cut(s) 281
BciT130I CCWGG 1 cut(s) 525
BcoDI GTCTC 5 cut(s) 9, 88, 144, 794, 1183
BglII AGATCT 1 cut(s) 645
BisI GCNGC 2 cut(s) 351, 906
BlpI GCTNAGC 1 cut(s) 909
BlsI GCNGC 2 cut(s) 352, 907
Bme1390I CCNGG 1 cut(s) 525
Bme18I GGWCC 2 cut(s) 430, 1213
BmgT120I GGNCC 2 cut(s) 430, 1213
BmiI GGNNCC 1 cut(s) 1153
BmrFI CCNGG 1 cut(s) 525
BmsI GCATC 5 cut(s) 138, 240, 597, 844, 1196
BoxI GACNNNNGTC 1 cut(s) 296
BpiI GAAGAC 2 cut(s) 66, 439
Bpu1102I GCTNAGC 1 cut(s) 909
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 222, 632
BsaI GGTCTC 1 cut(s) 794
BsaJI CCNNGG 3 cut(s) 524, 744, 1155
BsaWI WCCGGW 1 cut(s) 1249
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse1I ACTGG 3 cut(s) 384, 1167, 1216
Bse21I CCTNAGG 1 cut(s) 972
BseAI TCCGGA 1 cut(s) 1249
BseBI CCWGG 1 cut(s) 525
BseDI CCNNGG 3 cut(s) 524, 744, 1155
BseGI GGATG 5 cut(s) 129, 205, 231, 262, 288
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 3 cut(s) 813, 900, 986
BseNI ACTGG 3 cut(s) 384, 1167, 1216
BseRI GAGGAG 3 cut(s) 50, 643, 651
BseXI GCAGC 2 cut(s) 337, 917
BsgI GTGCAG 1 cut(s) 659
BshFI GGCC 1 cut(s) 770
BshNI GGYRCC 1 cut(s) 1151
BsiSI CCGG 2 cut(s) 428, 1250
BslFI GGGAC 1 cut(s) 1173
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 5 cut(s) 9, 88, 144, 794, 1183
BsmBI CGTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 1173
BsmI GAATGC 2 cut(s) 954, 972
BsnI GGCC 1 cut(s) 770
Bso31I GGTCTC 1 cut(s) 794
Bsp119I TTCGAA 1 cut(s) 201
Bsp13I TCCGGA 1 cut(s) 1249
Bsp143I GATC 3 cut(s) 328, 645, 739
Bsp1720I GCTNAGC 1 cut(s) 909
Bsp19I CCATGG 1 cut(s) 744
BspACI CCGC 4 cut(s) 87, 294, 712, 832
BspANI GGCC 1 cut(s) 770
BspCNI CTCAG 3 cut(s) 814, 901, 985
BspEI TCCGGA 1 cut(s) 1249
BspHI TCATGA 2 cut(s) 397, 664
BspLI GGNNCC 1 cut(s) 1153
BspPI GGATC 2 cut(s) 323, 734
BspT104I TTCGAA 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 1151
BspTNI GGTCTC 1 cut(s) 794
BsrI ACTGG 3 cut(s) 384, 1167, 1216
BssECI CCNNGG 3 cut(s) 524, 744, 1155
BssMI GATC 3 cut(s) 328, 645, 739
BssT1I CCWWGG 2 cut(s) 744, 1155
Bst2UI CCWGG 1 cut(s) 525
Bst4CI ACNGT 3 cut(s) 109, 137, 1277
Bst6I CTCTTC 3 cut(s) 27, 48, 1186
BstBI TTCGAA 1 cut(s) 201
BstDEI CTNAG 5 cut(s) 247, 698, 822, 909, 972
BstDSI CCRYGG 1 cut(s) 744
BstF5I GGATG 5 cut(s) 129, 205, 231, 262, 288
BstKTI GATC 3 cut(s) 331, 648, 742
BstMAI GTCTC 5 cut(s) 9, 88, 144, 794, 1183
BstMBI GATC 3 cut(s) 328, 645, 739
BstMWI GCNNNNNNNGC 3 cut(s) 126, 720, 1252
BstNI CCWGG 1 cut(s) 525
BstNSI RCATGY 1 cut(s) 1099
BstPAI GACNNNNGTC 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 523
BstV1I GCAGC 2 cut(s) 337, 917
BstV2I GAAGAC 2 cut(s) 66, 439
BstX2I RGATCY 3 cut(s) 328, 645, 739
BstXI CCANNNNNNTGG 1 cut(s) 1162
BstYI RGATCY 3 cut(s) 328, 645, 739
Bsu36I CCTNAGG 1 cut(s) 972
BsuRI GGCC 1 cut(s) 770
BtgI CCRYGG 1 cut(s) 744
BtsCI GGATG 5 cut(s) 129, 205, 231, 262, 288
CaiI CAGNNNCTG 2 cut(s) 1106, 1196
CciI TCATGA 2 cut(s) 397, 664
Cfr13I GGNCC 2 cut(s) 430, 1213
Csp6I GTAC 2 cut(s) 417, 1152
CviAII CATG 6 cut(s) 224, 398, 665, 745, 785, 1096
CviQI GTAC 2 cut(s) 417, 1152
DdeI CTNAG 5 cut(s) 247, 698, 822, 909, 972
DpnI GATC 3 cut(s) 330, 647, 741
DpnII GATC 3 cut(s) 328, 645, 739
Eam1104I CTCTTC 3 cut(s) 27, 48, 1186
EarI CTCTTC 3 cut(s) 27, 48, 1186
EciI GGCGGA 2 cut(s) 76, 701
Eco130I CCWWGG 2 cut(s) 744, 1155
Eco31I GGTCTC 1 cut(s) 794
Eco32I GATATC 1 cut(s) 1063
Eco47I GGWCC 2 cut(s) 430, 1213
Eco57I CTGAAG 3 cut(s) 195, 302, 455
Eco81I CCTNAGG 1 cut(s) 972
EcoRII CCWGG 1 cut(s) 523
EcoRV GATATC 1 cut(s) 1063
EcoT14I CCWWGG 2 cut(s) 744, 1155
EcoT22I ATGCAT 1 cut(s) 859
ErhI CCWWGG 2 cut(s) 744, 1155
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 6 cut(s) 227, 401, 668, 748, 788, 1099
FaqI GGGAC 1 cut(s) 1173
FatI CATG 6 cut(s) 223, 397, 664, 744, 784, 1095
Fnu4HI GCNGC 2 cut(s) 351, 906
FokI GGATG 5 cut(s) 116, 192, 218, 269, 295
Fsp4HI GCNGC 2 cut(s) 351, 906
GluI GCNGC 2 cut(s) 351, 906
HaeIII GGCC 1 cut(s) 770
HapII CCGG 2 cut(s) 428, 1250
Hin1II CATG 6 cut(s) 227, 401, 668, 748, 788, 1099
HincII GTYRAC 3 cut(s) 657, 840, 1075
HindII GTYRAC 3 cut(s) 657, 840, 1075
HinfI GANTC 8 cut(s) 144, 191, 411, 614, 658, 1024, 1103, 1193
HpaI GTTAAC 1 cut(s) 1075
HpaII CCGG 2 cut(s) 428, 1250
HphI GGTGA 2 cut(s) 17, 113
Hpy166II GTNNAC 5 cut(s) 475, 657, 840, 1075, 1234
Hpy188I TCNGA 7 cut(s) 14, 149, 214, 578, 1049, 1192, 1198
Hpy188III TCNNGA 8 cut(s) 141, 398, 590, 665, 851, 963, 974, 1250
Hpy8I GTNNAC 5 cut(s) 475, 657, 840, 1075, 1234
HpyAV CCTTC 5 cut(s) 219, 326, 430, 857, 1044
HpyCH4III ACNGT 3 cut(s) 109, 137, 1277
HpyCH4IV ACGT 1 cut(s) 991
HpyCH4V TGCA 7 cut(s) 231, 443, 640, 723, 736, 857, 954
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 720, 1252
HpyF3I CTNAG 5 cut(s) 247, 698, 822, 909, 972
HpySE526I ACGT 1 cut(s) 991
Hsp92II CATG 6 cut(s) 227, 401, 668, 748, 788, 1099
Kpn2I TCCGGA 1 cut(s) 1249
KpnI GGTACC 1 cut(s) 1155
KspAI GTTAAC 1 cut(s) 1075
Kzo9I GATC 3 cut(s) 328, 645, 739
LmnI GCTCC 3 cut(s) 37, 1243, 1252
Lsp1109I GCAGC 2 cut(s) 337, 917
LweI GCATC 5 cut(s) 138, 240, 597, 844, 1196
MaeII ACGT 1 cut(s) 991
MaeIII GTNAC 4 cut(s) 26, 615, 935, 992
MalI GATC 3 cut(s) 330, 647, 741
MboI GATC 3 cut(s) 328, 645, 739
MboII GAAGA 7 cut(s) 44, 65, 71, 167, 439, 989, 1173
MfeI CAATTG 1 cut(s) 333
MflI RGATCY 3 cut(s) 328, 645, 739
MlyI GAGTC 3 cut(s) 623, 652, 1187
MmeI TCCRAC 2 cut(s) 496, 1027
Mph1103I ATGCAT 1 cut(s) 859
MroI TCCGGA 1 cut(s) 1249
MseI TTAA 6 cut(s) 111, 651, 1074, 1086, 1206, 1303
MspA1I CMGCKG 1 cut(s) 908
MspI CCGG 2 cut(s) 428, 1250
MspR9I CCNGG 1 cut(s) 525
MunI CAATTG 1 cut(s) 333
Mva1269I GAATGC 2 cut(s) 954, 972
MvaI CCWGG 1 cut(s) 525
MwoI GCNNNNNNNGC 3 cut(s) 126, 720, 1252
NcoI CCATGG 1 cut(s) 744
NdeII GATC 3 cut(s) 328, 645, 739
NlaIII CATG 6 cut(s) 227, 401, 668, 748, 788, 1099
NlaIV GGNNCC 1 cut(s) 1153
NmuCI GTSAC 1 cut(s) 615
NsiI ATGCAT 1 cut(s) 859
NspI RCATGY 1 cut(s) 1099
NspV TTCGAA 1 cut(s) 201
PagI TCATGA 2 cut(s) 397, 664
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PctI GAATGC 2 cut(s) 954, 972
PfeI GAWTC 5 cut(s) 144, 191, 411, 1024, 1103
PflMI CCANNNNNTGG 1 cut(s) 338
PkrI GCNGC 2 cut(s) 352, 907
PleI GAGTC 3 cut(s) 622, 652, 1187
PpsI GAGTC 3 cut(s) 622, 652, 1187
PshAI GACNNNNGTC 1 cut(s) 296
PshBI ATTAAT 1 cut(s) 1086
Psp1406I AACGTT 1 cut(s) 991
Psp6I CCWGG 1 cut(s) 523
PspGI CCWGG 1 cut(s) 523
PspN4I GGNNCC 1 cut(s) 1153
PspPI GGNCC 2 cut(s) 430, 1213
PstNI CAGNNNCTG 2 cut(s) 1106, 1196
PsuI RGATCY 3 cut(s) 328, 645, 739
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 2 cut(s) 418, 1153
RsaNI GTAC 2 cut(s) 417, 1152
SaqAI TTAA 6 cut(s) 111, 651, 1074, 1086, 1206, 1303
SatI GCNGC 2 cut(s) 351, 906
Sau3AI GATC 3 cut(s) 328, 645, 739
Sau96I GGNCC 2 cut(s) 430, 1213
SchI GAGTC 3 cut(s) 623, 652, 1187
ScrFI CCNGG 1 cut(s) 525
SfaNI GCATC 5 cut(s) 138, 240, 597, 844, 1196
SfuI TTCGAA 1 cut(s) 201
SinI GGWCC 2 cut(s) 430, 1213
SmlI CTYRAG 2 cut(s) 237, 611
SmoI CTYRAG 2 cut(s) 237, 611
SsiI CCGC 4 cut(s) 87, 294, 712, 832
SspI AATATT 1 cut(s) 534
StyD4I CCNGG 1 cut(s) 523
StyI CCWWGG 2 cut(s) 744, 1155
TaaI ACNGT 3 cut(s) 109, 137, 1277
TaiI ACGT 1 cut(s) 994
TaqI TCGA 4 cut(s) 45, 178, 201, 1065
TfiI GAWTC 5 cut(s) 144, 191, 411, 1024, 1103
Tru1I TTAA 6 cut(s) 111, 651, 1074, 1086, 1206, 1303
Tru9I TTAA 6 cut(s) 111, 651, 1074, 1086, 1206, 1303
TseFI GTSAC 1 cut(s) 615
TseI GCWGC 2 cut(s) 350, 905
Tsp45I GTSAC 1 cut(s) 615
TspDTI ATGAA 5 cut(s) 66, 414, 510, 609, 773
TspGWI ACGGA 1 cut(s) 70
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 2 cut(s) 430, 1213
VspI ATTAAT 1 cut(s) 1086
XapI RAATTY 4 cut(s) 401, 728, 750, 1000
XceI RCATGY 1 cut(s) 1099
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.