Rorug06G0278600

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001445
Physical Location & Seq
Reverse (-)
42615133 .. 42616219
1087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0278600.1

Sequence Viewer

Length: 747 bp
ATGAGGCTATGGAAGAAGCCACATCTTTACAGTTTACCCCATTCTGATGCATACACCATAAATGGCCAGCCAGGGGATCTTTGTCCATGCTCCAACGAGACAACATATTGTCGAAAAGTGGATTATGGCAAGACGTATCTTCTTCGTATAGTCAATGCAAACATAGATGCATACCTTTTCTTCGCAGTTGCTCAACACAATCTCACTGTTGTTGGACTAGACGGAGTCTATATCAAACCCATATACACAACCTACATACTCATAAGCCCTGGACAAACTATGGATGTTTTACTCAAAGCAGATCAGGTTCTTGGGCGCTATTACATGGCTGGTAGACATTTCTCTAGTGATAATGCCAAAGTTTCTGAATTTGACCATGTCAATGTTACTGCAATCTTGGAATCCAATGGCAACTATATTTATCCAACATCTCCTCTTTTTCCCAAAACCCTTCCTATGTACTTAGACTTTGCAGCATCATTAAACTTCACTTACCAAATTAAAAGCTTAGCCTCCCCAGAATATCCAATAAATGTCCCACCAGACAATGACATTACAACCAGAATGTTTATAACAGCTTCCATGAATTCACTCTACTGTGAAGATTCAGGCTTCTTGAGCTGCGTGGAGATACCCGTTGCTACAAGTGTAAATAACATTAGCTGGGTGAACCCGAGGAGCACAGATCTCTTGCAAGCTTACTACAGGTTCAATCCTTTGAACTTGCCTGCATCCTTTGAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

248

Amino Acids

27.95

Weight (kDa)

5.68

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase PF00394 13 - 137 1.9e-30 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 572
AccI GTMKAC 1 cut(s) 334
AclWI GGATC 1 cut(s) 84
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 2 cut(s) 368, 586
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 3 cut(s) 712, 721, 740
AjnI CCWGG 2 cut(s) 70, 268
AluBI AGCT 5 cut(s) 507, 578, 621, 663, 698
AluI AGCT 5 cut(s) 507, 578, 621, 663, 698
Alw21I GWGCWC 1 cut(s) 683
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 1 cut(s) 84
Ama87I CYCGRG 1 cut(s) 673
AoxI GGCC 1 cut(s) 64
ApeKI GCWGC 2 cut(s) 473, 621
ApoI RAATTY 2 cut(s) 368, 586
AspLEI GCGC 1 cut(s) 318
AsuHPI GGTGA 1 cut(s) 679
AvaI CYCGRG 1 cut(s) 673
BalI TGGCCA 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 683
BbvI GCAGC 2 cut(s) 485, 608
BciT130I CCWGG 2 cut(s) 72, 270
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 2 cut(s) 218, 345
BfmI CTRYAG 1 cut(s) 703
BfoI RGCGCY 1 cut(s) 319
BglII AGATCT 1 cut(s) 685
BisI GCNGC 2 cut(s) 474, 622
BlpI GCTNAGC 1 cut(s) 508
BlsI GCNGC 2 cut(s) 475, 623
Bme1390I CCNGG 2 cut(s) 72, 270
BmeT110I CYCGRG 1 cut(s) 673
BmrFI CCNGG 2 cut(s) 72, 270
BmsI GCATC 4 cut(s) 37, 157, 485, 740
Bpu1102I GCTNAGC 1 cut(s) 508
BpuEI CTTGAG 1 cut(s) 637
BsaJI CCNNGG 3 cut(s) 71, 268, 674
Bsc4I CCNNNNNNNGG 1 cut(s) 73
BseBI CCWGG 2 cut(s) 72, 270
BseDI CCNNGG 3 cut(s) 71, 268, 674
BseGI GGATG 2 cut(s) 289, 731
BseLI CCNNNNNNNGG 1 cut(s) 73
BseRI GAGGAG 2 cut(s) 423, 691
BseXI GCAGC 2 cut(s) 485, 608
BseYI CCCAGC 1 cut(s) 663
BshFI GGCC 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 683
BsiHKCI CYCGRG 1 cut(s) 673
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 1 cut(s) 521
BsnI GGCC 1 cut(s) 66
BsoBI CYCGRG 1 cut(s) 673
Bsp1286I GDGCHC 1 cut(s) 683
Bsp143I GATC 3 cut(s) 76, 301, 685
Bsp1720I GCTNAGC 1 cut(s) 508
BspANI GGCC 1 cut(s) 66
BspPI GGATC 1 cut(s) 84
BssECI CCNNGG 3 cut(s) 71, 268, 674
BssMI GATC 3 cut(s) 76, 301, 685
Bst2UI CCWGG 2 cut(s) 72, 270
Bst4CI ACNGT 3 cut(s) 32, 208, 599
BstC8I GCNNGC 3 cut(s) 68, 696, 729
BstDEI CTNAG 2 cut(s) 463, 508
BstF5I GGATG 2 cut(s) 289, 731
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 3 cut(s) 79, 304, 688
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 3 cut(s) 76, 301, 685
BstMWI GCNNNNNNNGC 1 cut(s) 618
BstNI CCWGG 2 cut(s) 72, 270
BstSCI CCNGG 2 cut(s) 70, 268
BstSFI CTRYAG 1 cut(s) 703
BstV1I GCAGC 2 cut(s) 485, 608
BstX2I RGATCY 2 cut(s) 76, 685
BstYI RGATCY 2 cut(s) 76, 685
BsuRI GGCC 1 cut(s) 66
BtsCI GGATG 2 cut(s) 289, 731
BtsIMutI CAGTG 1 cut(s) 204
Cac8I GCNNGC 3 cut(s) 68, 696, 729
CfoI GCGC 1 cut(s) 318
Csp6I GTAC 1 cut(s) 460
CviAII CATG 4 cut(s) 87, 325, 377, 583
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 2 cut(s) 463, 508
DpnI GATC 3 cut(s) 78, 303, 687
DpnII GATC 3 cut(s) 76, 301, 685
EaeI YGGCCR 1 cut(s) 64
Eco88I CYCGRG 1 cut(s) 673
EcoRI GAATTC 1 cut(s) 586
EcoRII CCWGG 2 cut(s) 70, 268
EcoT22I ATGCAT 2 cut(s) 52, 172
FaeI CATG 4 cut(s) 90, 328, 380, 586
FaqI GGGAC 1 cut(s) 521
FatI CATG 4 cut(s) 86, 324, 376, 582
FblI GTMKAC 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 474, 622
FokI GGATG 2 cut(s) 296, 718
Fsp4HI GCNGC 2 cut(s) 474, 622
FspBI CTAG 2 cut(s) 218, 345
GlaI GCGC 1 cut(s) 317
GluI GCNGC 2 cut(s) 474, 622
GsaI CCCAGC 1 cut(s) 667
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 1 cut(s) 66
HhaI GCGC 1 cut(s) 318
Hin1II CATG 4 cut(s) 90, 328, 380, 586
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HindIII AAGCTT 2 cut(s) 505, 696
HinfI GANTC 4 cut(s) 225, 401, 605, 740
HphI GGTGA 1 cut(s) 679
Hpy166II GTNNAC 3 cut(s) 35, 335, 670
Hpy188I TCNGA 2 cut(s) 46, 367
Hpy188III TCNNGA 2 cut(s) 616, 744
Hpy8I GTNNAC 3 cut(s) 35, 335, 670
HpyAV CCTTC 1 cut(s) 461
HpyCH4III ACNGT 3 cut(s) 32, 208, 599
HpyCH4IV ACGT 1 cut(s) 134
HpyCH4V TGCA 7 cut(s) 50, 158, 170, 392, 473, 694, 731
HpyF10VI GCNNNNNNNGC 1 cut(s) 618
HpyF3I CTNAG 2 cut(s) 463, 508
HpySE526I ACGT 1 cut(s) 134
Hsp92II CATG 4 cut(s) 90, 328, 380, 586
HspAI GCGC 1 cut(s) 316
Kzo9I GATC 3 cut(s) 76, 301, 685
LmnI GCTCC 2 cut(s) 95, 678
Lsp1109I GCAGC 2 cut(s) 485, 608
LweI GCATC 4 cut(s) 37, 157, 485, 740
MaeI CTAG 2 cut(s) 218, 345
MaeII ACGT 1 cut(s) 134
MaeIII GTNAC 1 cut(s) 385
MalI GATC 3 cut(s) 78, 303, 687
MboI GATC 3 cut(s) 76, 301, 685
MboII GAAGA 5 cut(s) 25, 131, 134, 172, 614
MflI RGATCY 2 cut(s) 76, 685
MhlI GDGCHC 1 cut(s) 683
MlsI TGGCCA 1 cut(s) 66
MluCI AATT 3 cut(s) 368, 498, 586
MluNI TGGCCA 1 cut(s) 66
MlyI GAGTC 1 cut(s) 234
MmeI TCCRAC 3 cut(s) 117, 193, 449
MnlI CCTC 3 cut(s) 444, 523, 669
Mox20I TGGCCA 1 cut(s) 66
Mph1103I ATGCAT 2 cut(s) 52, 172
MscI TGGCCA 1 cut(s) 66
MseI TTAA 2 cut(s) 482, 501
MslI CAYNNNNRTG 2 cut(s) 45, 381
Msp20I TGGCCA 1 cut(s) 66
MspR9I CCNGG 2 cut(s) 72, 270
MvaI CCWGG 2 cut(s) 72, 270
MwoI GCNNNNNNNGC 1 cut(s) 618
NdeII GATC 3 cut(s) 76, 301, 685
NlaIII CATG 4 cut(s) 90, 328, 380, 586
NsiI ATGCAT 2 cut(s) 52, 172
PfeI GAWTC 3 cut(s) 401, 605, 740
PflFI GACNNNGTC 2 cut(s) 224, 377
PkrI GCNGC 2 cut(s) 475, 623
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
PsiI TTATAA 1 cut(s) 572
Psp6I CCWGG 2 cut(s) 70, 268
PspFI CCCAGC 1 cut(s) 663
PspGI CCWGG 2 cut(s) 70, 268
PsuI RGATCY 2 cut(s) 76, 685
PsyI GACNNNGTC 2 cut(s) 224, 377
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
RseI CAYNNNNRTG 2 cut(s) 45, 381
SaqAI TTAA 2 cut(s) 482, 501
SatI GCNGC 2 cut(s) 474, 622
Sau3AI GATC 3 cut(s) 76, 301, 685
SchI GAGTC 1 cut(s) 234
ScrFI CCNGG 2 cut(s) 72, 270
SduI GDGCHC 1 cut(s) 683
SfaNI GCATC 4 cut(s) 37, 157, 485, 740
SfcI CTRYAG 1 cut(s) 703
SmiMI CAYNNNNRTG 2 cut(s) 45, 381
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
Sse9I AATT 3 cut(s) 368, 498, 586
SspMI CTAG 2 cut(s) 218, 345
StyD4I CCNGG 2 cut(s) 70, 268
TaaI ACNGT 3 cut(s) 32, 208, 599
TaiI ACGT 1 cut(s) 137
TaqI TCGA 1 cut(s) 112
TasI AATT 3 cut(s) 368, 498, 586
TatI WGTACW 1 cut(s) 459
TfiI GAWTC 3 cut(s) 401, 605, 740
Tru1I TTAA 2 cut(s) 482, 501
Tru9I TTAA 2 cut(s) 482, 501
TscAI CASTG 1 cut(s) 211
TseI GCWGC 2 cut(s) 473, 621
TspDTI ATGAA 1 cut(s) 599
TspGWI ACGGA 1 cut(s) 237
TspRI CASTG 1 cut(s) 211
Tth111I GACNNNGTC 2 cut(s) 224, 377
XapI RAATTY 2 cut(s) 368, 586
XmiI GTMKAC 1 cut(s) 334
XspI CTAG 2 cut(s) 218, 345
Zsp2I ATGCAT 2 cut(s) 52, 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.