Rh6AG388800

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001445
Physical Location & Seq
Forward (+)
58764230 .. 58768421
4192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG388800.1

Sequence Viewer

Length: 1281 bp
ATGGGCGAAACAAGGGAAGATGTCTACGACGAAGAGCTCGTCGATTACGAAGAGGATGACGAGAAGGCCCCTGACTCCGCTGCTAAAGTCAATGGCGAATCCGCCAAGAAGGGATATGTTGGAATTCACAGCTCAGGATTCAGAGATTTCCTCTTGAAGCCGGAGCTTCTACGTGCTATTGTGGACTCGGGATTTGAGCATCCCTCTGAAGTACAACATGAATGCATCCCACAAGCTATTCTGGGAATGGATGTTATCTGCCAAGCTAAATCTGGGATGGGGAAAACGGCTGTATTTGTCCTATCTACTTTGCAGCAGATTGACCCTGTCGCGGGCCAAGTTTCTGCACTTATTCTTTGCCATACTCGAGAATTAGCTTACCAGATTTGCCATGAGTTTGAGAGATTCAGTACCTACTTGCCAGATCTTAAGGTTGCTGTCTTCTATGGTGGTGTCACTATCAAAAATCACAAAGATTTACTTAAAAATGAATGCCCCCACATTGTTGTTGGAACTCCTGGAAGAATACTAGCACTTGCAAGAGATAAAGACCTCTCTTTGAAGAATGTGAGGCATTTTATCCTCGATGAATGTGACAAGATGCTTGAGTCACTTGACATGAGGAGAGATGTGCAAGAGATTTTCAAGATGACGCCCCATGATAAGCAAGTAATGATGTTCTCTGCAACACTCAGCAAAGATATCCGACCGGTTTGCAAGAAATTTATGCAAGATCCTATGGAAATATATGTTGATGACGAAGCGAAGTTGACTCTTCATGGTCTAGTACAGCACTACATCAAGCTAACCGAGGCAGAGAAAAATCGCAAGCTGAATGATCTCCTTGATGCACTGGACTTCAACCAGGTTGTCATCTTCGTCAAAAGTGTGAGCAGAGCTGCAGAGTTGAACAAGTTACTCTCAGAGTGTAATTTTCCCTCTATATGCATCCATTCTGGAATGAACCAAGAAGAAAGGTTGAAGCGCTACAAAGGATTCAAGGAGGGTCAGAACAGGATTCTCGTGGCCACCGATTTGGTTGGTAGAGGAATTGATATTGAACGTGTCAATATTGTTATCAATTATGACATGCCAGACTCTGCGGACACCTACTTGCACAGGGTTGGCAGAGCTGGTAGGTTTGGCACCAAAGGGCTGGCAATTACATTTGTATCATCTGCTTCGGACTCTCAAGTCCTTAATGATGTTCAGGAGAGGTTTGAGGTTGACATAAAGGAGCTTCCTGAACAGATTGATACTGCGACATACATGCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

426

Amino Acids

48.12

Weight (kDa)

5.34

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 70 - 235 5.6e-41 DEAD/DEAH box helicase
ResIII PF04851 85 - 232 2.1e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 274 - 382 6.8e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1193
AccB1I GGYRCC 1 cut(s) 1145
AccI GTMKAC 1 cut(s) 24
AccII CGCG 1 cut(s) 332
AciI CCGC 4 cut(s) 78, 102, 332, 1103
AclWI GGATC 1 cut(s) 728
AcoI YGGCCR 1 cut(s) 1026
AcsI RAATTY 2 cut(s) 123, 722
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 653
AfaI GTAC 3 cut(s) 213, 412, 789
AfeI AGCGCT 1 cut(s) 986
AfiI CCNNNNNNNGG 2 cut(s) 331, 332
AflII CTTAAG 1 cut(s) 428
AflIII ACRYGT 1 cut(s) 1063
AgeI ACCGGT 1 cut(s) 709
AgsI TTSAA 8 cut(s) 157, 562, 646, 862, 910, 982, 1000, 1061
AjnI CCWGG 2 cut(s) 517, 864
Alw21I GWGCWC 1 cut(s) 39
AlwI GGATC 1 cut(s) 728
AlwNI CAGNNNCTG 1 cut(s) 1100
Ama87I CYCGRG 2 cut(s) 187, 366
Aor51HI AGCGCT 1 cut(s) 986
AoxI GGCC 3 cut(s) 66, 334, 1026
ApeKI GCWGC 3 cut(s) 80, 313, 899
ApoI RAATTY 2 cut(s) 123, 722
ArsI GACNNNNNNTTYG 4 cut(s) 24, 56, 176, 208
AsiGI ACCGGT 1 cut(s) 709
AspLEI GCGC 1 cut(s) 987
AspS9I GGNCC 2 cut(s) 67, 334
AvaI CYCGRG 2 cut(s) 187, 366
BaeI ACNNNNGTAYC 2 cut(s) 1155, 1188
BalI TGGCCA 1 cut(s) 1028
BanI GGYRCC 1 cut(s) 1145
BanII GRGCYC 1 cut(s) 39
BauI CACGAG 1 cut(s) 1022
BbsI GAAGAC 1 cut(s) 433
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 3 cut(s) 67, 325, 886
BccI CCATC 1 cut(s) 271
BceAI ACGGC 1 cut(s) 303
BcgI CGANNNNNNTGC 3 cut(s) 696, 730, 1252
BciT130I CCWGG 2 cut(s) 519, 866
BfaI CTAG 2 cut(s) 530, 785
BfmI CTRYAG 1 cut(s) 900
BfoI RGCGCY 1 cut(s) 988
BfrI CTTAAG 1 cut(s) 428
BglII AGATCT 1 cut(s) 424
BisI GCNGC 3 cut(s) 81, 314, 900
BlsI GCNGC 3 cut(s) 82, 315, 901
Bme1390I CCNGG 2 cut(s) 519, 866
BmeT110I CYCGRG 2 cut(s) 187, 366
BmgT120I GGNCC 2 cut(s) 67, 334
BmiI GGNNCC 2 cut(s) 69, 1147
BmrFI CCNGG 2 cut(s) 519, 866
BmsI GCATC 5 cut(s) 208, 234, 591, 838, 957
BpiI GAAGAC 1 cut(s) 433
BplI GAGNNNNNCTC 4 cut(s) 135, 167, 188, 220
Bpu10I CCTNAGC 1 cut(s) 133
BpuEI CTTGAG 2 cut(s) 626, 1176
BsaAI YACGTR 1 cut(s) 173
BsaHI GRCGYC 1 cut(s) 653
BsaJI CCNNGG 1 cut(s) 810
BsaWI WCCGGW 1 cut(s) 709
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 332
Bse118I RCCGGY 1 cut(s) 709
Bse1I ACTGG 1 cut(s) 858
BseBI CCWGG 2 cut(s) 519, 866
BseDI CCNNGG 1 cut(s) 810
BseGI GGATG 6 cut(s) 61, 199, 225, 256, 282, 948
BseLI CCNNNNNNNGG 2 cut(s) 331, 332
BseMII CTCAG 3 cut(s) 147, 706, 936
BseNI ACTGG 1 cut(s) 858
BseRI GAGGAG 1 cut(s) 637
BseXI GCAGC 3 cut(s) 67, 325, 886
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 1 cut(s) 332
Bsh1285I CGRYCG 1 cut(s) 710
BshFI GGCC 3 cut(s) 68, 336, 1028
BshNI GGYRCC 1 cut(s) 1145
BshTI ACCGGT 1 cut(s) 709
BsiEI CGRYCG 1 cut(s) 710
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 2 cut(s) 187, 366
BsiSI CCGG 2 cut(s) 161, 710
BslI CCNNNNNNNGG 2 cut(s) 331, 332
BsmI GAATGC 2 cut(s) 227, 497
BsnI GGCC 3 cut(s) 68, 336, 1028
BsoBI CYCGRG 2 cut(s) 187, 366
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 3 cut(s) 424, 733, 838
BspACI CCGC 4 cut(s) 78, 102, 332, 1103
BspANI GGCC 3 cut(s) 68, 336, 1028
BspCNI CTCAG 3 cut(s) 146, 705, 935
BspFNI CGCG 1 cut(s) 332
BspLI GGNNCC 2 cut(s) 69, 1147
BspMAI CTGCAG 1 cut(s) 904
BspPI GGATC 1 cut(s) 728
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 1145
BspTI CTTAAG 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 709
BsrI ACTGG 1 cut(s) 858
BssAI RCCGGY 1 cut(s) 709
BssECI CCNNGG 1 cut(s) 810
BssMI GATC 3 cut(s) 424, 733, 838
BssNI GRCGYC 1 cut(s) 653
BssSI CACGAG 1 cut(s) 1022
Bst2BI CACGAG 1 cut(s) 1022
Bst2UI CCWGG 2 cut(s) 519, 866
Bst6I CTCTTC 3 cut(s) 27, 45, 780
BstACI GRCGYC 1 cut(s) 653
BstAFI CTTAAG 1 cut(s) 428
BstBAI YACGTR 1 cut(s) 173
BstC8I GCNNGC 3 cut(s) 334, 830, 1158
BstDEI CTNAG 3 cut(s) 133, 692, 922
BstF5I GGATG 6 cut(s) 61, 199, 225, 256, 282, 948
BstFNI CGCG 1 cut(s) 332
BstH2I RGCGCY 1 cut(s) 988
BstHHI GCGC 1 cut(s) 987
BstKTI GATC 3 cut(s) 427, 736, 841
BstMBI GATC 3 cut(s) 424, 733, 838
BstMCI CGRYCG 1 cut(s) 710
BstNI CCWGG 2 cut(s) 519, 866
BstNSI RCATGY 2 cut(s) 1093, 1273
BstSCI CCNGG 2 cut(s) 517, 864
BstSFI CTRYAG 1 cut(s) 900
BstUI CGCG 1 cut(s) 332
BstV1I GCAGC 3 cut(s) 67, 325, 886
BstV2I GAAGAC 1 cut(s) 433
BstX2I RGATCY 2 cut(s) 424, 733
BstXI CCANNNNNNTGG 2 cut(s) 1036, 1156
BstYI RGATCY 2 cut(s) 424, 733
BsuRI GGCC 3 cut(s) 68, 336, 1028
BtsCI GGATG 6 cut(s) 61, 199, 225, 256, 282, 948
BtsIMutI CAGTG 1 cut(s) 851
Cac8I GCNNGC 3 cut(s) 334, 830, 1158
CaiI CAGNNNCTG 1 cut(s) 1100
CfoI GCGC 1 cut(s) 987
Cfr10I RCCGGY 1 cut(s) 709
Cfr13I GGNCC 2 cut(s) 67, 334
CseI GACGC 1 cut(s) 661
CsiI ACCWGGT 1 cut(s) 864
Csp6I GTAC 3 cut(s) 212, 411, 788
CspAI ACCGGT 1 cut(s) 709
CviAII CATG 7 cut(s) 218, 392, 619, 659, 779, 1090, 1270
CviQI GTAC 3 cut(s) 212, 411, 788
DdeI CTNAG 3 cut(s) 133, 692, 922
DpnI GATC 3 cut(s) 426, 735, 840
DpnII GATC 3 cut(s) 424, 733, 838
DrdI GACNNNNNNGTC 1 cut(s) 1193
DseDI GACNNNNNNGTC 1 cut(s) 1193
EaeI YGGCCR 1 cut(s) 1026
Eam1104I CTCTTC 3 cut(s) 27, 45, 780
EarI CTCTTC 3 cut(s) 27, 45, 780
EciI GGCGGA 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 39
Eco32I GATATC 1 cut(s) 703
Eco47III AGCGCT 1 cut(s) 986
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 228
Eco88I CYCGRG 2 cut(s) 187, 366
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 123
EcoRII CCWGG 2 cut(s) 517, 864
EcoRV GATATC 1 cut(s) 703
EcoT22I ATGCAT 2 cut(s) 227, 950
EcoT38I GRGCYC 1 cut(s) 39
FaeI CATG 7 cut(s) 221, 395, 622, 662, 782, 1093, 1273
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FatI CATG 7 cut(s) 217, 391, 618, 658, 778, 1089, 1269
FauI CCCGC 1 cut(s) 325
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 3 cut(s) 81, 314, 900
FokI GGATG 6 cut(s) 68, 186, 212, 263, 289, 935
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 3 cut(s) 81, 314, 900
FspBI CTAG 2 cut(s) 530, 785
GlaI GCGC 1 cut(s) 986
GluI GCNGC 3 cut(s) 81, 314, 900
HaeII RGCGCY 1 cut(s) 988
HaeIII GGCC 3 cut(s) 68, 336, 1028
HapII CCGG 2 cut(s) 161, 710
HgaI GACGC 1 cut(s) 661
HhaI GCGC 1 cut(s) 987
Hin1I GRCGYC 1 cut(s) 653
Hin1II CATG 7 cut(s) 221, 395, 622, 662, 782, 1093, 1273
Hin6I GCGC 1 cut(s) 985
HinP1I GCGC 1 cut(s) 985
HincII GTYRAC 2 cut(s) 771, 1228
HindII GTYRAC 2 cut(s) 771, 1228
HpaII CCGG 2 cut(s) 161, 710
Hpy166II GTNNAC 4 cut(s) 25, 184, 771, 1228
Hpy188I TCNGA 6 cut(s) 143, 208, 707, 925, 1011, 1186
Hpy188III TCNNGA 9 cut(s) 135, 154, 189, 368, 646, 957, 1211, 1244, 1278
Hpy8I GTNNAC 4 cut(s) 25, 184, 771, 1228
Hpy99I CGWCG 2 cut(s) 32, 44
HpyAV CCTTC 2 cut(s) 58, 103
HpyCH4IV ACGT 2 cut(s) 172, 1063
HpyF3I CTNAG 3 cut(s) 133, 692, 922
HpySE526I ACGT 2 cut(s) 172, 1063
Hsp92I GRCGYC 1 cut(s) 653
Hsp92II CATG 7 cut(s) 221, 395, 622, 662, 782, 1093, 1273
HspAI GCGC 1 cut(s) 985
Kzo9I GATC 3 cut(s) 424, 733, 838
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 2 cut(s) 163, 1237
Lsp1109I GCAGC 3 cut(s) 67, 325, 886
LweI GCATC 5 cut(s) 208, 234, 591, 838, 957
MabI ACCWGGT 1 cut(s) 864
MaeI CTAG 2 cut(s) 530, 785
MaeII ACGT 2 cut(s) 172, 1063
MaeIII GTNAC 4 cut(s) 454, 593, 609, 915
MalI GATC 3 cut(s) 426, 735, 840
MboI GATC 3 cut(s) 424, 733, 838
MboII GAAGA 9 cut(s) 29, 44, 62, 433, 534, 574, 767, 868, 983
MflI RGATCY 2 cut(s) 424, 733
MhlI GDGCHC 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 1028
MluCI AATT 7 cut(s) 123, 371, 722, 931, 1050, 1081, 1161
MluNI TGGCCA 1 cut(s) 1028
MlyI GAGTC 6 cut(s) 68, 179, 617, 766, 1091, 1181
MmeI TCCRAC 3 cut(s) 100, 490, 730
Mox20I TGGCCA 1 cut(s) 1028
Mph1103I ATGCAT 2 cut(s) 227, 950
MscI TGGCCA 1 cut(s) 1028
MseI TTAA 3 cut(s) 429, 483, 1200
Msp20I TGGCCA 1 cut(s) 1028
MspA1I CMGCKG 1 cut(s) 80
MspCI CTTAAG 1 cut(s) 428
MspI CCGG 2 cut(s) 161, 710
MspR9I CCNGG 2 cut(s) 519, 866
Mva1269I GAATGC 2 cut(s) 227, 497
MvaI CCWGG 2 cut(s) 519, 866
MvnI CGCG 1 cut(s) 332
NdeII GATC 3 cut(s) 424, 733, 838
NlaIII CATG 7 cut(s) 221, 395, 622, 662, 782, 1093, 1273
NlaIV GGNNCC 2 cut(s) 69, 1147
NmuCI GTSAC 3 cut(s) 454, 593, 609
NsiI ATGCAT 2 cut(s) 227, 950
NspI RCATGY 2 cut(s) 1093, 1273
PaeR7I CTCGAG 1 cut(s) 366
PciSI GCTCTTC 1 cut(s) 27
PcsI WCGNNNNNNNCGW 3 cut(s) 36, 45, 1029
PctI GAATGC 2 cut(s) 227, 497
PfeI GAWTC 5 cut(s) 98, 138, 405, 996, 1018
PflFI GACNNNGTC 1 cut(s) 326
PfoI TCCNGGA 1 cut(s) 517
PinAI ACCGGT 1 cut(s) 709
PkrI GCNGC 3 cut(s) 82, 315, 901
PleI GAGTC 6 cut(s) 68, 179, 616, 766, 1091, 1181
PpsI GAGTC 6 cut(s) 68, 179, 616, 766, 1091, 1181
Ppu21I YACGTR 1 cut(s) 173
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 2 cut(s) 517, 864
PspGI CCWGG 2 cut(s) 517, 864
PspN4I GGNNCC 2 cut(s) 69, 1147
PspPI GGNCC 2 cut(s) 67, 334
PstI CTGCAG 1 cut(s) 904
PstNI CAGNNNCTG 1 cut(s) 1100
PsuI RGATCY 2 cut(s) 424, 733
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 3 cut(s) 213, 412, 789
RsaNI GTAC 3 cut(s) 212, 411, 788
SacI GAGCTC 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 3 cut(s) 429, 483, 1200
SatI GCNGC 3 cut(s) 81, 314, 900
Sau3AI GATC 3 cut(s) 424, 733, 838
Sau96I GGNCC 2 cut(s) 67, 334
SchI GAGTC 6 cut(s) 68, 179, 617, 766, 1091, 1181
ScrFI CCNGG 2 cut(s) 519, 866
SduI GDGCHC 1 cut(s) 39
SexAI ACCWGGT 1 cut(s) 864
SfaNI GCATC 5 cut(s) 208, 234, 591, 838, 957
SfcI CTRYAG 1 cut(s) 900
Sfr274I CTCGAG 1 cut(s) 366
SlaI CTCGAG 1 cut(s) 366
SmlI CTYRAG 4 cut(s) 366, 428, 605, 1191
SmoI CTYRAG 4 cut(s) 366, 428, 605, 1191
Sse9I AATT 7 cut(s) 123, 371, 722, 931, 1050, 1081, 1161
SsiI CCGC 4 cut(s) 78, 102, 332, 1103
SspI AATATT 1 cut(s) 1072
SspMI CTAG 2 cut(s) 530, 785
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 517, 864
TaiI ACGT 2 cut(s) 175, 1066
TaqI TCGA 3 cut(s) 42, 367, 585
TasI AATT 7 cut(s) 123, 371, 722, 931, 1050, 1081, 1161
TatI WGTACW 2 cut(s) 211, 787
TfiI GAWTC 5 cut(s) 98, 138, 405, 996, 1018
Tru1I TTAA 3 cut(s) 429, 483, 1200
Tru9I TTAA 3 cut(s) 429, 483, 1200
TscAI CASTG 1 cut(s) 858
TseFI GTSAC 3 cut(s) 454, 593, 609
TseI GCWGC 3 cut(s) 80, 313, 899
Tsp45I GTSAC 3 cut(s) 454, 593, 609
TspDTI ATGAA 5 cut(s) 234, 504, 603, 767, 977
TspRI CASTG 1 cut(s) 858
Tth111I GACNNNGTC 1 cut(s) 326
Vha464I CTTAAG 1 cut(s) 428
XapI RAATTY 2 cut(s) 123, 722
XceI RCATGY 2 cut(s) 1093, 1273
XcmI CCANNNNNNNNNTGG 2 cut(s) 269, 506
XhoI CTCGAG 1 cut(s) 366
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 2 cut(s) 530, 785
Zsp2I ATGCAT 2 cut(s) 227, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.