Rh6BG397000

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001445
Physical Location & Seq
Forward (+)
62495561 .. 62501300
5740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG397000.1

Sequence Viewer

Length: 1137 bp
ATGGGCGAAACAAGGGAAGATGTCTACGACGAAGAGCTCGTCGATTACGAAGAGGATGACGAGAAGGCCCCTGACTCCGCAGCTAAAGTCAATGGCGAATCCGCCAAGAAGGGATATGTTGGAATTCACAGCTCAGGATTCAGAGATTTCCTCTTAAAGCCGGAGCTTCTTCGTGCTATTGTGGACTCGGGATTTGAGCATCCTTCTGAAGTACAACATGAATGCATCCCACAAGCTATTCTGGGAATGGATGTTATCTGCCAAGCTAAATCTGGGATGGGGAAAACAGCTGTATTTGTCCTATCTACTTTGCAGCAGATTGACCCTGTCGCAGGCCAAGTTTCTGCCCTTATTCTTTGCCATACTCGAGAATTAGCTTACCAGATTTGCCATGAGTTTGAGAGATTCAGTACCTACTTGCCAGATCTTAAGGTTGCTGTCTTCTATGGTGGTGTCACTATCAAAAATCACAAAGATTTACTCAAAAATGAATGCCCCCACATTGTTGTTGGAACTCCTGGAAGAATACTAGCACTTGCAAGAGATAAAGACCTCTCTTTGAAGAATGTGAGGCATTTTATCCTCGATGAATGTGACAAGATGCTTGAGTCACTTGACATGAGGAGAGATGTGCAAGAGATTTTCAAGATGACGCCCCATGATAAGCAAGTAATGATGTTCTCTGCAACACTCAGCAAAGATATCCGACCGGTTTGCAAGAAATTTATGCAAGATCCTATGGAAATATATGTTGATGACGAAGCCAAGTTGACTCTTCATGGTCTAGTACAGCACTACATCAAGCTAACCGAGGCAGAGAAAAATCGCAAGCTGAATGATCTCCTTGATGCACTGGACTTCAACCAGGTTGTCATCTTCGTCAAAAGTGTGAGCAGAGCTGCAGAGTTGAACAAGTTACTCTCAGAGTGTAATTTTCCCTCTATATGCATCCATTCTGGAATGAACCAAGAAGAAAGGTTGAAGCGCTACAAAGGATTCAAGGAGGGTCAGAACAGGATTCTCGTGGCCACCGATTTGGTTGGTAGAGGAATTGATATTGAACGTGTCAATATTGTTATCAATTATGACATGCCAGACTCTGCGGACACCTACTTGCACAGGGTATGTCAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

378

Amino Acids

42.94

Weight (kDa)

5.51

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 70 - 235 4.3e-41 DEAD/DEAH box helicase
ResIII PF04851 85 - 232 1.6e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 274 - 375 7.4e-25 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 24
AciI CCGC 3 cut(s) 78, 102, 1103
AclWI GGATC 1 cut(s) 728
AcoI YGGCCR 1 cut(s) 1026
AcsI RAATTY 2 cut(s) 123, 722
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 653
AfaI GTAC 3 cut(s) 213, 412, 789
AfeI AGCGCT 1 cut(s) 986
AfiI CCNNNNNNNGG 1 cut(s) 332
AflII CTTAAG 1 cut(s) 428
AflIII ACRYGT 1 cut(s) 1063
AgeI ACCGGT 1 cut(s) 709
AgsI TTSAA 7 cut(s) 562, 646, 862, 910, 982, 1000, 1061
AjnI CCWGG 2 cut(s) 517, 864
Alw21I GWGCWC 1 cut(s) 39
AlwI GGATC 1 cut(s) 728
AlwNI CAGNNNCTG 1 cut(s) 1100
Ama87I CYCGRG 2 cut(s) 187, 366
Aor51HI AGCGCT 1 cut(s) 986
AoxI GGCC 3 cut(s) 66, 334, 1026
ApeKI GCWGC 3 cut(s) 80, 313, 899
ApoI RAATTY 2 cut(s) 123, 722
ArsI GACNNNNNNTTYG 4 cut(s) 24, 56, 176, 208
AsiGI ACCGGT 1 cut(s) 709
AspLEI GCGC 1 cut(s) 987
AspS9I GGNCC 1 cut(s) 67
AvaI CYCGRG 2 cut(s) 187, 366
BalI TGGCCA 1 cut(s) 1028
BanII GRGCYC 1 cut(s) 39
BauI CACGAG 1 cut(s) 1022
BbsI GAAGAC 1 cut(s) 433
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 3 cut(s) 92, 325, 886
BccI CCATC 1 cut(s) 271
BcgI CGANNNNNNTGC 2 cut(s) 696, 730
BciT130I CCWGG 2 cut(s) 519, 866
BfaI CTAG 2 cut(s) 530, 785
BfmI CTRYAG 1 cut(s) 900
BfoI RGCGCY 1 cut(s) 988
BfrI CTTAAG 1 cut(s) 428
BglII AGATCT 1 cut(s) 424
BisI GCNGC 3 cut(s) 81, 314, 900
BlsI GCNGC 3 cut(s) 82, 315, 901
Bme1390I CCNGG 2 cut(s) 519, 866
BmeT110I CYCGRG 2 cut(s) 187, 366
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 2 cut(s) 519, 866
BmsI GCATC 5 cut(s) 208, 234, 591, 838, 957
BpiI GAAGAC 1 cut(s) 433
BplI GAGNNNNNCTC 2 cut(s) 135, 167
Bpu10I CCTNAGC 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 626
BsaHI GRCGYC 1 cut(s) 653
BsaJI CCNNGG 1 cut(s) 810
BsaWI WCCGGW 1 cut(s) 709
Bsc4I CCNNNNNNNGG 1 cut(s) 332
Bse118I RCCGGY 1 cut(s) 709
Bse1I ACTGG 1 cut(s) 858
BseBI CCWGG 2 cut(s) 519, 866
BseDI CCNNGG 1 cut(s) 810
BseGI GGATG 6 cut(s) 61, 199, 225, 256, 282, 948
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMII CTCAG 3 cut(s) 147, 706, 936
BseNI ACTGG 1 cut(s) 858
BseRI GAGGAG 1 cut(s) 637
BseXI GCAGC 3 cut(s) 92, 325, 886
Bsh1285I CGRYCG 1 cut(s) 710
BshFI GGCC 3 cut(s) 68, 336, 1028
BshTI ACCGGT 1 cut(s) 709
BsiEI CGRYCG 1 cut(s) 710
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 2 cut(s) 187, 366
BsiSI CCGG 2 cut(s) 161, 710
BslI CCNNNNNNNGG 1 cut(s) 332
BsmI GAATGC 2 cut(s) 227, 497
BsnI GGCC 3 cut(s) 68, 336, 1028
BsoBI CYCGRG 2 cut(s) 187, 366
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 3 cut(s) 424, 733, 838
BspACI CCGC 3 cut(s) 78, 102, 1103
BspANI GGCC 3 cut(s) 68, 336, 1028
BspCNI CTCAG 3 cut(s) 146, 705, 935
BspLI GGNNCC 1 cut(s) 69
BspMAI CTGCAG 1 cut(s) 904
BspPI GGATC 1 cut(s) 728
BspQI GCTCTTC 1 cut(s) 27
BspTI CTTAAG 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 709
BsrI ACTGG 1 cut(s) 858
BssAI RCCGGY 1 cut(s) 709
BssECI CCNNGG 1 cut(s) 810
BssMI GATC 3 cut(s) 424, 733, 838
BssNI GRCGYC 1 cut(s) 653
BssSI CACGAG 1 cut(s) 1022
Bst2BI CACGAG 1 cut(s) 1022
Bst2UI CCWGG 2 cut(s) 519, 866
Bst6I CTCTTC 3 cut(s) 27, 45, 780
BstACI GRCGYC 1 cut(s) 653
BstAFI CTTAAG 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 334, 830
BstDEI CTNAG 3 cut(s) 133, 692, 922
BstENI CCTNNNNNAGG 1 cut(s) 330
BstF5I GGATG 6 cut(s) 61, 199, 225, 256, 282, 948
BstH2I RGCGCY 1 cut(s) 988
BstHHI GCGC 1 cut(s) 987
BstKTI GATC 3 cut(s) 427, 736, 841
BstMBI GATC 3 cut(s) 424, 733, 838
BstMCI CGRYCG 1 cut(s) 710
BstNI CCWGG 2 cut(s) 519, 866
BstNSI RCATGY 1 cut(s) 1093
BstSCI CCNGG 2 cut(s) 517, 864
BstSFI CTRYAG 1 cut(s) 900
BstV1I GCAGC 3 cut(s) 92, 325, 886
BstV2I GAAGAC 1 cut(s) 433
BstX2I RGATCY 2 cut(s) 424, 733
BstXI CCANNNNNNTGG 1 cut(s) 1036
BstYI RGATCY 2 cut(s) 424, 733
BsuRI GGCC 3 cut(s) 68, 336, 1028
BtsCI GGATG 6 cut(s) 61, 199, 225, 256, 282, 948
BtsIMutI CAGTG 1 cut(s) 851
Cac8I GCNNGC 2 cut(s) 334, 830
CaiI CAGNNNCTG 1 cut(s) 1100
CfoI GCGC 1 cut(s) 987
Cfr10I RCCGGY 1 cut(s) 709
Cfr13I GGNCC 1 cut(s) 67
CseI GACGC 1 cut(s) 661
CsiI ACCWGGT 1 cut(s) 864
Csp6I GTAC 3 cut(s) 212, 411, 788
CspAI ACCGGT 1 cut(s) 709
CviAII CATG 6 cut(s) 218, 392, 619, 659, 779, 1090
CviQI GTAC 3 cut(s) 212, 411, 788
DdeI CTNAG 3 cut(s) 133, 692, 922
DpnI GATC 3 cut(s) 426, 735, 840
DpnII GATC 3 cut(s) 424, 733, 838
EaeI YGGCCR 1 cut(s) 1026
Eam1104I CTCTTC 3 cut(s) 27, 45, 780
EarI CTCTTC 3 cut(s) 27, 45, 780
EciI GGCGGA 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 39
Eco32I GATATC 1 cut(s) 703
Eco47III AGCGCT 1 cut(s) 986
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 228
Eco88I CYCGRG 2 cut(s) 187, 366
EcoICRI GAGCTC 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 123
EcoRII CCWGG 2 cut(s) 517, 864
EcoRV GATATC 1 cut(s) 703
EcoT22I ATGCAT 2 cut(s) 227, 950
EcoT38I GRGCYC 1 cut(s) 39
FaeI CATG 6 cut(s) 221, 395, 622, 662, 782, 1093
FatI CATG 6 cut(s) 217, 391, 618, 658, 778, 1089
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 3 cut(s) 81, 314, 900
FokI GGATG 6 cut(s) 68, 186, 212, 263, 289, 935
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 3 cut(s) 81, 314, 900
FspBI CTAG 2 cut(s) 530, 785
GlaI GCGC 1 cut(s) 986
GluI GCNGC 3 cut(s) 81, 314, 900
HaeII RGCGCY 1 cut(s) 988
HaeIII GGCC 3 cut(s) 68, 336, 1028
HapII CCGG 2 cut(s) 161, 710
HgaI GACGC 1 cut(s) 661
HhaI GCGC 1 cut(s) 987
Hin1I GRCGYC 1 cut(s) 653
Hin1II CATG 6 cut(s) 221, 395, 622, 662, 782, 1093
Hin6I GCGC 1 cut(s) 985
HinP1I GCGC 1 cut(s) 985
HincII GTYRAC 1 cut(s) 771
HindII GTYRAC 1 cut(s) 771
HpaII CCGG 2 cut(s) 161, 710
Hpy166II GTNNAC 3 cut(s) 25, 184, 771
Hpy188I TCNGA 5 cut(s) 143, 208, 707, 925, 1011
Hpy188III TCNNGA 5 cut(s) 135, 189, 368, 646, 957
Hpy8I GTNNAC 3 cut(s) 25, 184, 771
Hpy99I CGWCG 2 cut(s) 32, 44
HpyAV CCTTC 3 cut(s) 58, 103, 213
HpyCH4IV ACGT 1 cut(s) 1063
HpyF3I CTNAG 3 cut(s) 133, 692, 922
HpySE526I ACGT 1 cut(s) 1063
Hsp92I GRCGYC 1 cut(s) 653
Hsp92II CATG 6 cut(s) 221, 395, 622, 662, 782, 1093
HspAI GCGC 1 cut(s) 985
Kzo9I GATC 3 cut(s) 424, 733, 838
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 1 cut(s) 163
Lsp1109I GCAGC 3 cut(s) 92, 325, 886
LweI GCATC 5 cut(s) 208, 234, 591, 838, 957
MabI ACCWGGT 1 cut(s) 864
MaeI CTAG 2 cut(s) 530, 785
MaeII ACGT 1 cut(s) 1063
MaeIII GTNAC 4 cut(s) 454, 593, 609, 915
MalI GATC 3 cut(s) 426, 735, 840
MboI GATC 3 cut(s) 424, 733, 838
MflI RGATCY 2 cut(s) 424, 733
MhlI GDGCHC 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 1028
MluCI AATT 7 cut(s) 123, 371, 722, 931, 1050, 1081, 1130
MluNI TGGCCA 1 cut(s) 1028
MlyI GAGTC 5 cut(s) 68, 179, 617, 766, 1091
MmeI TCCRAC 3 cut(s) 100, 490, 730
Mox20I TGGCCA 1 cut(s) 1028
Mph1103I ATGCAT 2 cut(s) 227, 950
MscI TGGCCA 1 cut(s) 1028
MseI TTAA 2 cut(s) 155, 429
Msp20I TGGCCA 1 cut(s) 1028
MspA1I CMGCKG 1 cut(s) 290
MspCI CTTAAG 1 cut(s) 428
MspI CCGG 2 cut(s) 161, 710
MspR9I CCNGG 2 cut(s) 519, 866
Mva1269I GAATGC 2 cut(s) 227, 497
MvaI CCWGG 2 cut(s) 519, 866
NdeII GATC 3 cut(s) 424, 733, 838
NlaIII CATG 6 cut(s) 221, 395, 622, 662, 782, 1093
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 3 cut(s) 454, 593, 609
NsiI ATGCAT 2 cut(s) 227, 950
NspI RCATGY 1 cut(s) 1093
PaeR7I CTCGAG 1 cut(s) 366
PciSI GCTCTTC 1 cut(s) 27
PcsI WCGNNNNNNNCGW 3 cut(s) 36, 45, 1029
PctI GAATGC 2 cut(s) 227, 497
PfeI GAWTC 5 cut(s) 98, 138, 405, 996, 1018
PflFI GACNNNGTC 1 cut(s) 326
PfoI TCCNGGA 1 cut(s) 517
PinAI ACCGGT 1 cut(s) 709
PkrI GCNGC 3 cut(s) 82, 315, 901
PleI GAGTC 5 cut(s) 68, 179, 616, 766, 1091
PpsI GAGTC 5 cut(s) 68, 179, 616, 766, 1091
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 2 cut(s) 517, 864
PspGI CCWGG 2 cut(s) 517, 864
PspN4I GGNNCC 1 cut(s) 69
PspPI GGNCC 1 cut(s) 67
PstI CTGCAG 1 cut(s) 904
PstNI CAGNNNCTG 1 cut(s) 1100
PsuI RGATCY 2 cut(s) 424, 733
PsyI GACNNNGTC 1 cut(s) 326
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 3 cut(s) 213, 412, 789
RsaNI GTAC 3 cut(s) 212, 411, 788
SacI GAGCTC 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 2 cut(s) 155, 429
SatI GCNGC 3 cut(s) 81, 314, 900
Sau3AI GATC 3 cut(s) 424, 733, 838
Sau96I GGNCC 1 cut(s) 67
SchI GAGTC 5 cut(s) 68, 179, 617, 766, 1091
ScrFI CCNGG 2 cut(s) 519, 866
SduI GDGCHC 1 cut(s) 39
SexAI ACCWGGT 1 cut(s) 864
SfaNI GCATC 5 cut(s) 208, 234, 591, 838, 957
SfcI CTRYAG 1 cut(s) 900
Sfr274I CTCGAG 1 cut(s) 366
SlaI CTCGAG 1 cut(s) 366
SmlI CTYRAG 3 cut(s) 366, 428, 605
SmoI CTYRAG 3 cut(s) 366, 428, 605
Sse9I AATT 7 cut(s) 123, 371, 722, 931, 1050, 1081, 1130
SsiI CCGC 3 cut(s) 78, 102, 1103
SspI AATATT 1 cut(s) 1072
SspMI CTAG 2 cut(s) 530, 785
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 517, 864
TaiI ACGT 1 cut(s) 1066
TaqI TCGA 3 cut(s) 42, 367, 585
TasI AATT 7 cut(s) 123, 371, 722, 931, 1050, 1081, 1130
TatI WGTACW 2 cut(s) 211, 787
TfiI GAWTC 5 cut(s) 98, 138, 405, 996, 1018
Tru1I TTAA 2 cut(s) 155, 429
Tru9I TTAA 2 cut(s) 155, 429
TscAI CASTG 1 cut(s) 858
TseFI GTSAC 3 cut(s) 454, 593, 609
TseI GCWGC 3 cut(s) 80, 313, 899
Tsp45I GTSAC 3 cut(s) 454, 593, 609
TspDTI ATGAA 5 cut(s) 234, 504, 603, 767, 977
TspRI CASTG 1 cut(s) 858
Tth111I GACNNNGTC 1 cut(s) 326
Vha464I CTTAAG 1 cut(s) 428
XagI CCTNNNNNAGG 1 cut(s) 330
XapI RAATTY 2 cut(s) 123, 722
XceI RCATGY 1 cut(s) 1093
XcmI CCANNNNNNNNNTGG 2 cut(s) 269, 506
XhoI CTCGAG 1 cut(s) 366
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 2 cut(s) 530, 785
Zsp2I ATGCAT 2 cut(s) 227, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.