RLG00000019493

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001445
Physical Location & Seq
Forward (+)
52748563 .. 52754276
5714 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019493

Sequence Viewer

Length: 1293 bp
ATGTACAGGATGGGTGAAATCAAAGACAGCGATGGGTATGAAGAAGAGCTCGTTGACTACGATGAGGAAGAACAAAACGCCCCCACCTCCGTCTCCGCCAAGCCCAATGGCGAATCCGCCAAAAAAACAGATTTTTTTTTTTTTCCCACATTATTGCCGATCTGGAAGCCATTTATACTTACTGCGGTTTCGTCCGGTACGATTTTCTTTGCTTCTTCATCAGTTCAACACGAGTGCATCCCTCAAGCTATTTTAGGAATGGATGTTCTTTGCCAAGCAAAATCTGGGATGGGCAAAACTGCAGTCTTTGTTCTGTCAACTCTTCAGCAAATAGATCCGGTTGCTGGTCAAGTTGCTGCTCTCATTCTATGCCACACAAGAGAATTGGCTTACCAGATTTGTCATGAATTTCAGAGATTCAGTACTTATTTGCCCGAGTTAAAAGTTGCGGTTTTCTATGGAGGTGTAACTATTAAGGTTCACAGAGATTTGCTGAAAAATGAATGCCCTCATATTGTTGTTGGAACACCTGGGAGAGTGTTGGCTTTGGCAAGAGATAAAGACCTTGGATTAAAGAATGTGAGGCATTTTATTCTTGATGAATGCGACAAGATGCTTGAATCACTTGATATGAGGAGAGATGTGCAGGAGATCTTCAGGTTGACTCCTCATGATAAGCAAGTAATGATGTTTTCAGCAACGCTAAGCAAGGAAATCCGCCCTGTCTGCAAGAAATTTATGCAAGATCCGATGGAAATTTATGTAGATGATGAAGCCAAGTTGACTCTTCATGGTCTCGTTCAGCATTACATCAAATTGAGTGAATCTGAGAAGAACCGCAAGTTGACCGACCTTCTTGATGCGTTGGATTTCAATCAAGTTGTTATTTTTGTAAAAAGTGTGAGCAGAGCAGCTGAGTTGAACAAGTTACTGGCTGACTGTAATTTCCCCTCTATCTGCATTCATTCTGGAATGCCCCAGGAAGAAAGGTTGAAAAGGTACAAGAATTTCAAGGAGGGCCTTTCAAGAATTCTTGTGGCAACAGATTTAGTAGGAAGAGGAATTGACATCGAGCGTGTTAACATTGTTATCAACTATGACATGCCAGATTCTGCTGATACCTACCTGCACAGGGTTGGTAGAGCCGGAAGGTTCGGTACTAAAGGGTTGGCAATAACATTTGTCTCATCCGCATCGGACTCTGATGTACTCAATCAGGTCCAGTCAAGGTTTGAAGTGGACATAAAGGAGCTCCCAGAGCAAATTGATACTTCTACATACATGCAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006974 GO:0007275 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009555 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:1901360 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

431

Amino Acids

48.58

Weight (kDa)

5.64

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 74 - 239 5e-40 DEAD/DEAH box helicase
ResIII PF04851 89 - 236 3.6e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 278 - 386 9.9e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1134
AciI CCGC 7 cut(s) 96, 117, 185, 449, 718, 838, 1191
AclWI GGATC 2 cut(s) 329, 740
AcsI RAATTY 5 cut(s) 407, 734, 756, 1006, 1029
AcuI CTGAAG 2 cut(s) 308, 640
AfaI GTAC 6 cut(s) 5, 199, 424, 1001, 1159, 1209
AfiI CCNNNNNNNGG 2 cut(s) 344, 1132
AgsI TTSAA 8 cut(s) 227, 620, 874, 922, 994, 1012, 1026, 1235
AjnI CCWGG 2 cut(s) 529, 978
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
AluBI AGCT 4 cut(s) 49, 248, 914, 1252
AluI AGCT 4 cut(s) 49, 248, 914, 1252
Alw21I GWGCWC 2 cut(s) 51, 1254
Alw26I GTCTC 3 cut(s) 97, 800, 1189
AlwI GGATC 2 cut(s) 329, 740
AlwNI CAGNNNCTG 1 cut(s) 1112
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 1 cut(s) 1018
ApeKI GCWGC 2 cut(s) 356, 911
ApoI RAATTY 5 cut(s) 407, 734, 756, 1006, 1029
ArsI GACNNNNNNTTYG 2 cut(s) 288, 320
AspS9I GGNCC 2 cut(s) 1018, 1219
AsuHPI GGTGA 1 cut(s) 26
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 1 cut(s) 1219
BanII GRGCYC 2 cut(s) 51, 1254
BarI GAAGNNNNNNTAC 2 cut(s) 1141, 1173
BauI CACGAG 1 cut(s) 230
Bbv12I GWGCWC 2 cut(s) 51, 1254
BbvI GCAGC 2 cut(s) 343, 923
BccI CCATC 4 cut(s) 4, 26, 283, 745
BciT130I CCWGG 2 cut(s) 531, 980
BcoDI GTCTC 3 cut(s) 97, 800, 1189
BfmI CTRYAG 1 cut(s) 300
BfuAI ACCTGC 1 cut(s) 1134
BglII AGATCT 1 cut(s) 651
BisI GCNGC 2 cut(s) 357, 912
BlpI GCTNAGC 1 cut(s) 704
BlsI GCNGC 2 cut(s) 358, 913
BmcAI AGTACT 1 cut(s) 424
Bme1390I CCNGG 2 cut(s) 531, 980
Bme18I GGWCC 1 cut(s) 1219
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 2 cut(s) 1018, 1219
BmrFI CCNGG 2 cut(s) 531, 980
BmsI GCATC 4 cut(s) 246, 603, 850, 1202
Bpu1102I GCTNAGC 1 cut(s) 704
BpuEI CTTGAG 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 873
BsaI GGTCTC 1 cut(s) 800
BsaJI CCNNGG 3 cut(s) 530, 565, 978
BsaWI WCCGGW 2 cut(s) 194, 337
Bsc4I CCNNNNNNNGG 2 cut(s) 344, 1132
Bse1I ACTGG 2 cut(s) 936, 1222
Bse8I GATNNNNATC 1 cut(s) 873
BseBI CCWGG 2 cut(s) 531, 980
BseDI CCNNGG 3 cut(s) 530, 565, 978
BseGI GGATG 5 cut(s) 15, 237, 268, 294, 1187
BseJI GATNNNNATC 1 cut(s) 873
BseLI CCNNNNNNNGG 2 cut(s) 344, 1132
BseMII CTCAG 2 cut(s) 819, 906
BseNI ACTGG 2 cut(s) 936, 1222
BseRI GAGGAG 2 cut(s) 649, 657
BseXI GCAGC 2 cut(s) 343, 923
BsgI GTGCAG 2 cut(s) 665, 1112
BshFI GGCC 1 cut(s) 1020
BsiHKAI GWGCWC 2 cut(s) 51, 1254
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 3 cut(s) 195, 338, 1146
BslI CCNNNNNNNGG 2 cut(s) 344, 1132
BsmAI GTCTC 3 cut(s) 97, 800, 1189
BsmBI CGTCTC 1 cut(s) 97
BsmI GAATGC 4 cut(s) 509, 608, 960, 978
BsnI GGCC 1 cut(s) 1020
Bso31I GGTCTC 1 cut(s) 800
BsoBI CYCGRG 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 51, 1254
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 4 cut(s) 159, 334, 651, 745
Bsp1720I GCTNAGC 1 cut(s) 704
BspACI CCGC 7 cut(s) 96, 117, 185, 449, 718, 838, 1191
BspANI GGCC 1 cut(s) 1020
BspCNI CTCAG 2 cut(s) 820, 907
BspHI TCATGA 2 cut(s) 403, 670
BspMAI CTGCAG 1 cut(s) 304
BspMI ACCTGC 1 cut(s) 1134
BspPI GGATC 2 cut(s) 329, 740
BspQI GCTCTTC 1 cut(s) 39
BspTNI GGTCTC 1 cut(s) 800
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 936, 1222
BssECI CCNNGG 3 cut(s) 530, 565, 978
BssMI GATC 4 cut(s) 159, 334, 651, 745
BssSI CACGAG 1 cut(s) 230
BssT1I CCWWGG 1 cut(s) 565
Bst2BI CACGAG 1 cut(s) 230
Bst2UI CCWGG 2 cut(s) 531, 980
Bst4CI ACNGT 1 cut(s) 941
Bst6I CTCTTC 4 cut(s) 39, 327, 792, 1051
BstAUI TGTACA 1 cut(s) 3
BstDEI CTNAG 3 cut(s) 704, 828, 915
BstF5I GGATG 5 cut(s) 15, 237, 268, 294, 1187
BstKTI GATC 4 cut(s) 162, 337, 654, 748
BstMAI GTCTC 3 cut(s) 97, 800, 1189
BstMBI GATC 4 cut(s) 159, 334, 651, 745
BstMWI GCNNNNNNNGC 2 cut(s) 726, 1258
BstNI CCWGG 2 cut(s) 531, 980
BstNSI RCATGY 2 cut(s) 1105, 1285
BstSCI CCNGG 2 cut(s) 529, 978
BstSFI CTRYAG 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 343, 923
BstX2I RGATCY 3 cut(s) 334, 651, 745
BstYI RGATCY 3 cut(s) 334, 651, 745
BsuRI GGCC 1 cut(s) 1020
BtgZI GCGATG 1 cut(s) 45
BtsCI GGATG 5 cut(s) 15, 237, 268, 294, 1187
BveI ACCTGC 1 cut(s) 1134
CaiI CAGNNNCTG 1 cut(s) 1112
CciI TCATGA 2 cut(s) 403, 670
Cfr13I GGNCC 2 cut(s) 1018, 1219
Csp6I GTAC 6 cut(s) 4, 198, 423, 1000, 1158, 1208
CviAII CATG 5 cut(s) 404, 671, 791, 1102, 1282
CviQI GTAC 6 cut(s) 4, 198, 423, 1000, 1158, 1208
DdeI CTNAG 3 cut(s) 704, 828, 915
DpnI GATC 4 cut(s) 161, 336, 653, 747
DpnII GATC 4 cut(s) 159, 334, 651, 745
Eam1104I CTCTTC 4 cut(s) 39, 327, 792, 1051
EarI CTCTTC 4 cut(s) 39, 327, 792, 1051
EciI GGCGGA 3 cut(s) 85, 106, 707
Ecl136II GAGCTC 2 cut(s) 49, 1252
Eco130I CCWWGG 1 cut(s) 565
Eco24I GRGCYC 2 cut(s) 51, 1254
Eco31I GGTCTC 1 cut(s) 800
Eco47I GGWCC 1 cut(s) 1219
Eco53kI GAGCTC 2 cut(s) 49, 1252
Eco57I CTGAAG 2 cut(s) 308, 640
Eco88I CYCGRG 1 cut(s) 434
EcoICRI GAGCTC 2 cut(s) 49, 1252
EcoO109I RGGNCCY 1 cut(s) 1018
EcoRI GAATTC 1 cut(s) 1029
EcoRII CCWGG 2 cut(s) 529, 978
EcoT14I CCWWGG 1 cut(s) 565
EcoT38I GRGCYC 2 cut(s) 51, 1254
ErhI CCWWGG 1 cut(s) 565
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 5 cut(s) 407, 674, 794, 1105, 1285
FatI CATG 5 cut(s) 403, 670, 790, 1101, 1281
Fnu4HI GCNGC 2 cut(s) 357, 912
FokI GGATG 5 cut(s) 22, 224, 275, 301, 1174
FriOI GRGCYC 2 cut(s) 51, 1254
Fsp4HI GCNGC 2 cut(s) 357, 912
GluI GCNGC 2 cut(s) 357, 912
HaeIII GGCC 1 cut(s) 1020
HapII CCGG 3 cut(s) 195, 338, 1146
Hin1II CATG 5 cut(s) 407, 674, 794, 1105, 1285
HincII GTYRAC 6 cut(s) 55, 318, 663, 783, 846, 1081
HindII GTYRAC 6 cut(s) 55, 318, 663, 783, 846, 1081
HinfI GANTC 8 cut(s) 113, 417, 620, 664, 784, 824, 1109, 1199
HpaI GTTAAC 1 cut(s) 1081
HpaII CCGG 3 cut(s) 195, 338, 1146
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 8 cut(s) 55, 318, 481, 663, 783, 846, 1081, 1240
Hpy188I TCNGA 5 cut(s) 414, 750, 829, 1198, 1204
Hpy188III TCNNGA 7 cut(s) 163, 404, 596, 671, 857, 969, 1026
Hpy8I GTNNAC 8 cut(s) 55, 318, 481, 663, 783, 846, 1081, 1240
HpyAV CCTTC 2 cut(s) 863, 1143
HpyCH4III ACNGT 1 cut(s) 941
HpyCH4V TGCA 8 cut(s) 237, 302, 646, 729, 742, 960, 1129, 1285
HpyF10VI GCNNNNNNNGC 2 cut(s) 726, 1258
HpyF3I CTNAG 3 cut(s) 704, 828, 915
Hsp92II CATG 5 cut(s) 407, 674, 794, 1105, 1285
KspAI GTTAAC 1 cut(s) 1081
Kzo9I GATC 4 cut(s) 159, 334, 651, 745
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 2 cut(s) 1249, 1257
Lsp1109I GCAGC 2 cut(s) 343, 923
LweI GCATC 4 cut(s) 246, 603, 850, 1202
MaeIII GTNAC 2 cut(s) 466, 927
MalI GATC 4 cut(s) 161, 336, 653, 747
MboI GATC 4 cut(s) 159, 334, 651, 745
MflI RGATCY 3 cut(s) 334, 651, 745
MhlI GDGCHC 2 cut(s) 51, 1254
MlyI GAGTC 3 cut(s) 658, 778, 1193
MmeI TCCRAC 2 cut(s) 502, 846
MseI TTAA 4 cut(s) 440, 474, 572, 1080
MslI CAYNNNNRTG 1 cut(s) 1286
MspA1I CMGCKG 1 cut(s) 914
MspI CCGG 3 cut(s) 195, 338, 1146
MspR9I CCNGG 2 cut(s) 531, 980
Mva1269I GAATGC 4 cut(s) 509, 608, 960, 978
MvaI CCWGG 2 cut(s) 531, 980
MwoI GCNNNNNNNGC 2 cut(s) 726, 1258
NdeII GATC 4 cut(s) 159, 334, 651, 745
NlaIII CATG 5 cut(s) 407, 674, 794, 1105, 1285
NspI RCATGY 2 cut(s) 1105, 1285
PagI TCATGA 2 cut(s) 403, 670
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 197
PctI GAATGC 4 cut(s) 509, 608, 960, 978
PfeI GAWTC 5 cut(s) 113, 417, 620, 824, 1109
PkrI GCNGC 2 cut(s) 358, 913
PleI GAGTC 3 cut(s) 658, 778, 1193
PpsI GAGTC 3 cut(s) 658, 778, 1193
Psp124BI GAGCTC 2 cut(s) 51, 1254
Psp6I CCWGG 2 cut(s) 529, 978
PspGI CCWGG 2 cut(s) 529, 978
PspPI GGNCC 2 cut(s) 1018, 1219
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 1112
PsuI RGATCY 3 cut(s) 334, 651, 745
PvuII CAGCTG 1 cut(s) 914
RsaI GTAC 6 cut(s) 5, 199, 424, 1001, 1159, 1209
RsaNI GTAC 6 cut(s) 4, 198, 423, 1000, 1158, 1208
RseI CAYNNNNRTG 1 cut(s) 1286
SacI GAGCTC 2 cut(s) 51, 1254
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 4 cut(s) 440, 474, 572, 1080
SatI GCNGC 2 cut(s) 357, 912
Sau3AI GATC 4 cut(s) 159, 334, 651, 745
Sau96I GGNCC 2 cut(s) 1018, 1219
ScaI AGTACT 1 cut(s) 424
SchI GAGTC 3 cut(s) 658, 778, 1193
ScrFI CCNGG 2 cut(s) 531, 980
SduI GDGCHC 2 cut(s) 51, 1254
SfaNI GCATC 4 cut(s) 246, 603, 850, 1202
SfcI CTRYAG 1 cut(s) 300
SinI GGWCC 1 cut(s) 1219
SmiMI CAYNNNNRTG 1 cut(s) 1286
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
SsiI CCGC 7 cut(s) 96, 117, 185, 449, 718, 838, 1191
SstI GAGCTC 2 cut(s) 51, 1254
StyD4I CCNGG 2 cut(s) 529, 978
StyI CCWWGG 1 cut(s) 565
TaaI ACNGT 1 cut(s) 941
TaqI TCGA 1 cut(s) 1071
TaqII GACCGA 1 cut(s) 863
TatI WGTACW 3 cut(s) 3, 422, 1207
TfiI GAWTC 5 cut(s) 113, 417, 620, 824, 1109
Tru1I TTAA 4 cut(s) 440, 474, 572, 1080
Tru9I TTAA 4 cut(s) 440, 474, 572, 1080
TseI GCWGC 2 cut(s) 356, 911
TspDTI ATGAA 8 cut(s) 54, 207, 420, 516, 615, 779, 786, 953
TspGWI ACGGA 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 1219
XapI RAATTY 5 cut(s) 407, 734, 756, 1006, 1029
XceI RCATGY 2 cut(s) 1105, 1285
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
ZrmI AGTACT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.