pycom09g14020

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001445
Physical Location & Seq
Reverse (-)
13264280 .. 13268297
4018 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g14020.1

Sequence Viewer

Length: 1035 bp
ATGGATGTTATTTGTCAAGCAAAATCTGGGATGGGAAAAACTGCCGTCTTTGTTCTATCAACCCTTCAGCAAATAGATCCAGTACCTGGTCAGGTTGCTGCTCTTATTCTTTGCCACACGAGAGAATTGGCTTACCAGATTTGTCATGAATTTGAGAGGTTCAGTACTTATTTGCCAGACCTGAAGGTTGCAGTTTTCTATGGAGGAGTCAATATCAAGGTTCACAAAGATTTGCTAAAAAACGAATGTCCTCACATTGTTGTTGGTACACCTGGGAGAGTATTAGCTTTGGCAAGAGACAAAGAGCTTGGACTGAAGAATGTCAGACATTTTATTCTTGATGAATGTGATAAGATGCTTGAGTCACTTGACATGAGAAGAGATGTGCAGGAGATCTTTAAGTTGACTCCTCATGACAAGCAGGTGATGATGTTTTCAGCAACACTAAGCAAGGAAATCCGCCCTGTTTGCAAGAAGTTTATGCAAGATCCCATGGAAATCTATGTAGATGATGAGGCCAAATTGACTCTTCATGGTCTCGTACAGCACTACATCAAATTGAGTGAGCCTGAGAAAAACCGCAAATTGACTGACCTCCTTGATGCATTGGATTTCAACCAAGTTGTTATTTTTGTAAAAAGTGTAAGCAGAGCAGCTGAGCTGAACAAGCTACTTGCTGATTGTAACTTCCCCTCCATCTGCATTCACTCTGGAATGCCGCAGGAAGAAAGGCTAAAACGTTACAAGAATTTCAAGGAGGGGCTTTCAAGAATTCTTGTGGCAACGGATTTAGTAGGAAGGGGAATTGACATCGAGCGTGTTAACATTGTTATCAATTATGACATGCCTGATTCTGCTGACACCTACTTACACAGGGTTGGCAGAGCTGGTAGGTTTGGTACCAAGGGCCTAGCAATTACATTTGTCTCTTCCGCATCTGATTCTGATGTTCTTAATCAGGTCCAGTCAAGGTTTGAGGTGGATATAAAGGAGCTTCCGGAGCAAATTGATACTTCTACTTACATGCAAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006974 GO:0007275 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009555 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:1901360 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

345

Amino Acids

38.96

Weight (kDa)

6.38

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 2 - 153 3.1e-36 DEAD/DEAH box helicase
ResIII PF04851 5 - 150 9.8e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 191 - 300 6.5e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 412
Acc36I ACCTGC 1 cut(s) 412
Acc65I GGTACC 1 cut(s) 899
AccB1I GGYRCC 1 cut(s) 899
AccB7I CCANNNNNTGG 1 cut(s) 86
AccIII TCCGGA 1 cut(s) 997
AciI CCGC 4 cut(s) 460, 580, 719, 933
AclI AACGTT 1 cut(s) 739
AclWI GGATC 2 cut(s) 71, 482
AcsI RAATTY 3 cut(s) 149, 748, 771
AcuI CTGAAG 3 cut(s) 50, 203, 335
AfaI GTAC 5 cut(s) 84, 166, 268, 543, 901
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 3 cut(s) 616, 754, 768
AjnI CCWGG 2 cut(s) 85, 271
AluBI AGCT 7 cut(s) 287, 307, 656, 661, 670, 887, 994
AluI AGCT 7 cut(s) 287, 307, 656, 661, 670, 887, 994
Alw26I GTCTC 3 cut(s) 291, 542, 931
AlwI GGATC 2 cut(s) 71, 482
AlwNI CAGNNNCTG 1 cut(s) 86
Aor13HI TCCGGA 1 cut(s) 997
AoxI GGCC 2 cut(s) 516, 907
ApeKI GCWGC 2 cut(s) 98, 653
ApoI RAATTY 3 cut(s) 149, 748, 771
Asp718I GGTACC 1 cut(s) 899
AspS9I GGNCC 2 cut(s) 907, 961
AsuHPI GGTGA 1 cut(s) 436
AvaII GGWCC 1 cut(s) 961
BanI GGYRCC 1 cut(s) 899
BauI CACGAG 1 cut(s) 118
BbvI GCAGC 2 cut(s) 85, 665
BccI CCATC 2 cut(s) 25, 704
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 2 cut(s) 87, 273
BcoDI GTCTC 3 cut(s) 291, 542, 931
BfaI CTAG 1 cut(s) 911
BfuAI ACCTGC 1 cut(s) 412
BglII AGATCT 1 cut(s) 393
BisI GCNGC 3 cut(s) 99, 654, 719
BlpI GCTNAGC 1 cut(s) 657
BlsI GCNGC 3 cut(s) 100, 655, 720
BmcAI AGTACT 1 cut(s) 166
Bme1390I CCNGG 2 cut(s) 87, 273
Bme18I GGWCC 1 cut(s) 961
BmgT120I GGNCC 2 cut(s) 907, 961
BmiI GGNNCC 1 cut(s) 901
BmrFI CCNGG 2 cut(s) 87, 273
BmsI GCATC 3 cut(s) 345, 592, 944
Bpu1102I GCTNAGC 1 cut(s) 657
BpuEI CTTGAG 1 cut(s) 380
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 3 cut(s) 272, 492, 903
BsaWI WCCGGW 1 cut(s) 997
BsaXI ACNNNNNCTCC 2 cut(s) 677, 707
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 80, 964
BseAI TCCGGA 1 cut(s) 997
BseBI CCWGG 2 cut(s) 87, 273
BseDI CCNNGG 3 cut(s) 272, 492, 903
BseGI GGATG 2 cut(s) 10, 36
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMII CTCAG 2 cut(s) 561, 648
BseNI ACTGG 2 cut(s) 80, 964
BseRI GAGGAG 2 cut(s) 219, 399
BseXI GCAGC 2 cut(s) 85, 665
BsgI GTGCAG 1 cut(s) 407
BshFI GGCC 2 cut(s) 518, 909
BshNI GGYRCC 1 cut(s) 899
BsiSI CCGG 1 cut(s) 998
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 3 cut(s) 291, 542, 931
BsmI GAATGC 2 cut(s) 702, 720
BsnI GGCC 2 cut(s) 518, 909
Bso31I GGTCTC 1 cut(s) 542
Bsp13I TCCGGA 1 cut(s) 997
Bsp143I GATC 3 cut(s) 76, 393, 487
Bsp1720I GCTNAGC 1 cut(s) 657
Bsp19I CCATGG 1 cut(s) 492
BspACI CCGC 4 cut(s) 460, 580, 719, 933
BspANI GGCC 2 cut(s) 518, 909
BspCNI CTCAG 2 cut(s) 562, 649
BspEI TCCGGA 1 cut(s) 997
BspHI TCATGA 2 cut(s) 145, 412
BspLI GGNNCC 1 cut(s) 901
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 2 cut(s) 71, 482
BspT107I GGYRCC 1 cut(s) 899
BspTNI GGTCTC 1 cut(s) 542
BsrI ACTGG 2 cut(s) 80, 964
BssECI CCNNGG 3 cut(s) 272, 492, 903
BssMI GATC 3 cut(s) 76, 393, 487
BssSI CACGAG 1 cut(s) 118
BssT1I CCWWGG 2 cut(s) 492, 903
Bst2BI CACGAG 1 cut(s) 118
Bst2UI CCWGG 2 cut(s) 87, 273
Bst6I CTCTTC 3 cut(s) 373, 534, 934
BstC8I GCNNGC 1 cut(s) 1029
BstDEI CTNAG 3 cut(s) 446, 570, 657
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 2 cut(s) 10, 36
BstKTI GATC 3 cut(s) 79, 396, 490
BstMAI GTCTC 3 cut(s) 291, 542, 931
BstMBI GATC 3 cut(s) 76, 393, 487
BstMWI GCNNNNNNNGC 3 cut(s) 468, 667, 1000
BstNI CCWGG 2 cut(s) 87, 273
BstNSI RCATGY 2 cut(s) 847, 1027
BstSCI CCNGG 2 cut(s) 85, 271
BstV1I GCAGC 2 cut(s) 85, 665
BstX2I RGATCY 3 cut(s) 76, 393, 487
BstYI RGATCY 3 cut(s) 76, 393, 487
BsuRI GGCC 2 cut(s) 518, 909
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 2 cut(s) 10, 36
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 1 cut(s) 1029
CaiI CAGNNNCTG 1 cut(s) 86
CciI TCATGA 2 cut(s) 145, 412
Cfr13I GGNCC 2 cut(s) 907, 961
CsiI ACCWGGT 1 cut(s) 85
Csp6I GTAC 5 cut(s) 83, 165, 267, 542, 900
CviAII CATG 7 cut(s) 146, 373, 413, 493, 533, 844, 1024
CviQI GTAC 5 cut(s) 83, 165, 267, 542, 900
DdeI CTNAG 3 cut(s) 446, 570, 657
DpnI GATC 3 cut(s) 78, 395, 489
DpnII GATC 3 cut(s) 76, 393, 487
Eam1104I CTCTTC 3 cut(s) 373, 534, 934
EarI CTCTTC 3 cut(s) 373, 534, 934
EciI GGCGGA 1 cut(s) 449
Eco130I CCWWGG 2 cut(s) 492, 903
Eco31I GGTCTC 1 cut(s) 542
Eco47I GGWCC 1 cut(s) 961
Eco57I CTGAAG 3 cut(s) 50, 203, 335
EcoO109I RGGNCCY 1 cut(s) 907
EcoRI GAATTC 1 cut(s) 771
EcoRII CCWGG 2 cut(s) 85, 271
EcoT14I CCWWGG 2 cut(s) 492, 903
EcoT22I ATGCAT 1 cut(s) 607
ErhI CCWWGG 2 cut(s) 492, 903
FaeI CATG 7 cut(s) 149, 376, 416, 496, 536, 847, 1027
FatI CATG 7 cut(s) 145, 372, 412, 492, 532, 843, 1023
Fnu4HI GCNGC 3 cut(s) 99, 654, 719
FokI GGATG 2 cut(s) 17, 43
Fsp4HI GCNGC 3 cut(s) 99, 654, 719
FspBI CTAG 1 cut(s) 911
GluI GCNGC 3 cut(s) 99, 654, 719
HaeIII GGCC 2 cut(s) 518, 909
HapII CCGG 1 cut(s) 998
Hin1II CATG 7 cut(s) 149, 376, 416, 496, 536, 847, 1027
HincII GTYRAC 2 cut(s) 405, 823
HindII GTYRAC 2 cut(s) 405, 823
HinfI GANTC 6 cut(s) 207, 362, 406, 526, 851, 941
HpaI GTTAAC 1 cut(s) 823
HpaII CCGG 1 cut(s) 998
HphI GGTGA 1 cut(s) 436
Hpy166II GTNNAC 4 cut(s) 223, 269, 405, 823
Hpy188I TCNGA 3 cut(s) 326, 940, 946
Hpy188III TCNNGA 6 cut(s) 146, 338, 413, 711, 768, 998
Hpy8I GTNNAC 4 cut(s) 223, 269, 405, 823
HpyAV CCTTC 3 cut(s) 74, 178, 792
HpyCH4IV ACGT 1 cut(s) 739
HpyCH4V TGCA 7 cut(s) 191, 388, 471, 484, 605, 702, 1027
HpyF10VI GCNNNNNNNGC 3 cut(s) 468, 667, 1000
HpyF3I CTNAG 3 cut(s) 446, 570, 657
HpySE526I ACGT 1 cut(s) 739
Hsp92II CATG 7 cut(s) 149, 376, 416, 496, 536, 847, 1027
Kpn2I TCCGGA 1 cut(s) 997
KpnI GGTACC 1 cut(s) 903
KspAI GTTAAC 1 cut(s) 823
Kzo9I GATC 3 cut(s) 76, 393, 487
LmnI GCTCC 2 cut(s) 991, 1000
Lsp1109I GCAGC 2 cut(s) 85, 665
LweI GCATC 3 cut(s) 345, 592, 944
MabI ACCWGGT 1 cut(s) 85
MaeI CTAG 1 cut(s) 911
MaeII ACGT 1 cut(s) 739
MaeIII GTNAC 3 cut(s) 363, 683, 740
MalI GATC 3 cut(s) 78, 395, 489
MboI GATC 3 cut(s) 76, 393, 487
MboII GAAGA 5 cut(s) 328, 390, 521, 737, 921
MflI RGATCY 3 cut(s) 76, 393, 487
MlyI GAGTC 4 cut(s) 216, 371, 400, 520
MnlI CCTC 9 cut(s) 150, 197, 261, 420, 508, 605, 703, 751, 970
Mph1103I ATGCAT 1 cut(s) 607
MroI TCCGGA 1 cut(s) 997
MseI TTAA 3 cut(s) 399, 822, 954
MspA1I CMGCKG 1 cut(s) 656
MspI CCGG 1 cut(s) 998
MspR9I CCNGG 2 cut(s) 87, 273
Mva1269I GAATGC 2 cut(s) 702, 720
MvaI CCWGG 2 cut(s) 87, 273
MwoI GCNNNNNNNGC 3 cut(s) 468, 667, 1000
NcoI CCATGG 1 cut(s) 492
NdeII GATC 3 cut(s) 76, 393, 487
NlaIII CATG 7 cut(s) 149, 376, 416, 496, 536, 847, 1027
NlaIV GGNNCC 1 cut(s) 901
NmuCI GTSAC 1 cut(s) 363
NsiI ATGCAT 1 cut(s) 607
NspI RCATGY 2 cut(s) 847, 1027
PagI TCATGA 2 cut(s) 145, 412
PaqCI CACCTGC 1 cut(s) 412
PctI GAATGC 2 cut(s) 702, 720
PfeI GAWTC 2 cut(s) 851, 941
PflMI CCANNNNNTGG 1 cut(s) 86
PkrI GCNGC 3 cut(s) 100, 655, 720
PleI GAGTC 4 cut(s) 215, 370, 400, 520
PpsI GAGTC 4 cut(s) 215, 370, 400, 520
Psp1406I AACGTT 1 cut(s) 739
Psp6I CCWGG 2 cut(s) 85, 271
PspGI CCWGG 2 cut(s) 85, 271
PspN4I GGNNCC 1 cut(s) 901
PspPI GGNCC 2 cut(s) 907, 961
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 3 cut(s) 76, 393, 487
PvuII CAGCTG 1 cut(s) 656
RsaI GTAC 5 cut(s) 84, 166, 268, 543, 901
RsaNI GTAC 5 cut(s) 83, 165, 267, 542, 900
SaqAI TTAA 3 cut(s) 399, 822, 954
SatI GCNGC 3 cut(s) 99, 654, 719
Sau3AI GATC 3 cut(s) 76, 393, 487
Sau96I GGNCC 2 cut(s) 907, 961
ScaI AGTACT 1 cut(s) 166
SchI GAGTC 4 cut(s) 216, 371, 400, 520
ScrFI CCNGG 2 cut(s) 87, 273
SexAI ACCWGGT 1 cut(s) 85
SfaNI GCATC 3 cut(s) 345, 592, 944
SinI GGWCC 1 cut(s) 961
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
SsiI CCGC 4 cut(s) 460, 580, 719, 933
SspMI CTAG 1 cut(s) 911
StyD4I CCNGG 2 cut(s) 85, 271
StyI CCWWGG 2 cut(s) 492, 903
TaiI ACGT 1 cut(s) 742
TaqI TCGA 1 cut(s) 813
TatI WGTACW 1 cut(s) 164
TauI GCSGC 1 cut(s) 721
TfiI GAWTC 2 cut(s) 851, 941
Tru1I TTAA 3 cut(s) 399, 822, 954
Tru9I TTAA 3 cut(s) 399, 822, 954
TseFI GTSAC 1 cut(s) 363
TseI GCWGC 2 cut(s) 98, 653
Tsp45I GTSAC 1 cut(s) 363
TspDTI ATGAA 3 cut(s) 162, 357, 521
TspGWI ACGGA 1 cut(s) 800
Van91I CCANNNNNTGG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 961
XapI RAATTY 3 cut(s) 149, 748, 771
XceI RCATGY 2 cut(s) 847, 1027
XspI CTAG 1 cut(s) 911
ZrmI AGTACT 1 cut(s) 166
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.