pycom15g11200

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001445
Physical Location & Seq
Reverse (-)
7568954 .. 7572287
3334 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g11200.2

Sequence Viewer

Length: 1110 bp
ATGGATGTTATTTGTCAAGCTAAATCTGGGATGGGAAAAACTGCTGTTTTTGTTCTATCATCTTTACAGCAGATTGACCCTGTCGCAGGCCAAGTCTCTGCCCTTGTCCTTTGCCATACTAGAGAATTAGCATACCAGATTTGTCATGAATTTGAGAGGTTCAGTACATACTTGCCTGATCTCAAGGTTGCTGTCTTCTATGGTGGTGTTAATATCAAACTTCACAAAGATTTACTGAAAAATGAATGCCCGCACATTGTTGTTGGAACTCCCGGAAGAATACTAGCACTGGCAAGAGATAAAGACCTCTCCTTGAAGAATGTGAGGCATTTTATCCTTGATGAATGCGACAAGATGCTTGAATCACTCGACATGAGGAGAGATGTGCAGGAGATCTTCAAGATCACGCCTCACGATAAGCAAGTGACGATGTTTTCCGCAACACTCAGCAAAGACATTCGCCCGGTTTGCAAGAAATTTATGCAAGATCCTATGGAAATATATGTTGATGATGAAGCCAAGTTGACCTTGCATGGTCTAGTACAGCACTACATCAAGTTAACCGAGGCAGAGAAGAACCGCAAGTTGAATGACCTCCTTGATGCATTGGACTTCAATCAAGTTGTTATATTCGTCAAAAGCGTTAGCAGAGCTGCTGAGCTGAACAAACTGCTTTCAGAGTGTAACTTTCCCTCTATATGCATCCATTCTGGAATGAGCCAAGAAGAAAGGTTGAAGCGGTACAAAGGATTCAAGGAGGGTCAGAACAGGATTCTCGTGGCAACTGATTTAGTCGGTAGAGGAATCGACATTGAGCGTGTTAACATTGTTATCAACTACGACATGCCAGATTCTGCTGACACTTATTTACACAGGGTTGGCAGAGCTGGTAGGTTTGGCACCAAAGGTCTTGCTATTACGTTTGTTTCATCCGCAGCAGACTCTGATGTTCTAAATGATGTTCAGGAGAGGTTTGAGGTTGACATAAAGGAGCTTCCTGAACAGATCGATACTGCCACATACAGTATGTTTTCTTTGCTGGATATTTTTTTTTCTGGTGTTTTGCTTTATAGTCAACTTTTTGTTTTTGGTCTAAAGGTCAGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006974 GO:0007275 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009555 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:1901360 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

370

Amino Acids

41.83

Weight (kDa)

6.38

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 899
AciI CCGC 5 cut(s) 251, 438, 580, 739, 933
AclWI GGATC 1 cut(s) 482
AcsI RAATTY 2 cut(s) 149, 476
AfaI GTAC 3 cut(s) 166, 543, 743
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 7 cut(s) 316, 362, 400, 589, 616, 736, 754
AluBI AGCT 5 cut(s) 20, 653, 661, 887, 994
AluI AGCT 5 cut(s) 20, 653, 661, 887, 994
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 1 cut(s) 482
AlwNI CAGNNNCTG 2 cut(s) 854, 944
AoxI GGCC 1 cut(s) 88
ApeKI GCWGC 2 cut(s) 653, 935
ApoI RAATTY 2 cut(s) 149, 476
AsuC2I CCSGG 2 cut(s) 273, 464
BanI GGYRCC 1 cut(s) 899
BauI CACGAG 1 cut(s) 776
BbsI GAAGAC 1 cut(s) 187
BbvI GCAGC 2 cut(s) 640, 947
BccI CCATC 1 cut(s) 25
BcnI CCSGG 2 cut(s) 273, 464
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 3 cut(s) 120, 284, 539
BglII AGATCT 1 cut(s) 393
BisI GCNGC 2 cut(s) 654, 936
BlpI GCTNAGC 1 cut(s) 657
BlsI GCNGC 2 cut(s) 655, 937
Bme1390I CCNGG 2 cut(s) 273, 464
BmiI GGNNCC 1 cut(s) 901
BmrFI CCNGG 2 cut(s) 273, 464
BmsI GCATC 3 cut(s) 345, 592, 711
BpiI GAAGAC 1 cut(s) 187
Bpu1102I GCTNAGC 1 cut(s) 657
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 2 cut(s) 273, 464
Bsa29I ATCGAT 1 cut(s) 1008
BsaJI CCNNGG 1 cut(s) 564
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 294
BseCI ATCGAT 1 cut(s) 1008
BseDI CCNNGG 1 cut(s) 564
BseGI GGATG 4 cut(s) 10, 36, 702, 929
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMII CTCAG 2 cut(s) 460, 648
BseNI ACTGG 1 cut(s) 294
BseRI GAGGAG 1 cut(s) 391
BseXI GCAGC 2 cut(s) 640, 947
BsgI GTGCAG 1 cut(s) 407
BshFI GGCC 1 cut(s) 90
BshNI GGYRCC 1 cut(s) 899
BshVI ATCGAT 1 cut(s) 1008
BsiSI CCGG 2 cut(s) 273, 464
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 1 cut(s) 100
BsmI GAATGC 2 cut(s) 251, 350
BsnI GGCC 1 cut(s) 90
Bsp143I GATC 5 cut(s) 178, 393, 402, 487, 1005
Bsp1720I GCTNAGC 1 cut(s) 657
BspACI CCGC 5 cut(s) 251, 438, 580, 739, 933
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 2 cut(s) 459, 649
BspDI ATCGAT 1 cut(s) 1008
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 901
BspPI GGATC 1 cut(s) 482
BspT107I GGYRCC 1 cut(s) 899
BsrI ACTGG 1 cut(s) 294
BssECI CCNNGG 1 cut(s) 564
BssMI GATC 5 cut(s) 178, 393, 402, 487, 1005
BssSI CACGAG 1 cut(s) 776
Bst2BI CACGAG 1 cut(s) 776
Bst4CI ACNGT 1 cut(s) 1025
BstC8I GCNNGC 2 cut(s) 88, 251
BstDEI CTNAG 2 cut(s) 446, 657
BstENI CCTNNNNNAGG 1 cut(s) 84
BstF5I GGATG 4 cut(s) 10, 36, 702, 929
BstKTI GATC 5 cut(s) 181, 396, 405, 490, 1008
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 5 cut(s) 178, 393, 402, 487, 1005
BstMWI GCNNNNNNNGC 1 cut(s) 468
BstNSI RCATGY 1 cut(s) 847
BstSCI CCNGG 2 cut(s) 271, 462
BstV1I GCAGC 2 cut(s) 640, 947
BstV2I GAAGAC 1 cut(s) 187
BstX2I RGATCY 2 cut(s) 393, 487
BstYI RGATCY 2 cut(s) 393, 487
Bsu15I ATCGAT 1 cut(s) 1008
BsuRI GGCC 1 cut(s) 90
BsuTUI ATCGAT 1 cut(s) 1008
BtsCI GGATG 4 cut(s) 10, 36, 702, 929
BtsIMutI CAGTG 1 cut(s) 287
Cac8I GCNNGC 2 cut(s) 88, 251
CaiI CAGNNNCTG 2 cut(s) 854, 944
CciI TCATGA 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 1008
Csp6I GTAC 3 cut(s) 165, 542, 742
CviAII CATG 4 cut(s) 146, 373, 533, 844
CviJI RGCY 8 cut(s) 20, 90, 518, 653, 661, 720, 887, 994
CviKI_1 RGCY 8 cut(s) 20, 90, 518, 653, 661, 720, 887, 994
CviQI GTAC 3 cut(s) 165, 542, 742
DdeI CTNAG 2 cut(s) 446, 657
DpnI GATC 5 cut(s) 180, 395, 404, 489, 1007
DpnII GATC 5 cut(s) 178, 393, 402, 487, 1005
EcoNI CCTNNNNNAGG 1 cut(s) 84
EcoT22I ATGCAT 2 cut(s) 607, 704
FaeI CATG 4 cut(s) 149, 376, 536, 847
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FatI CATG 4 cut(s) 145, 372, 532, 843
FauI CCCGC 1 cut(s) 258
Fnu4HI GCNGC 2 cut(s) 654, 936
FokI GGATG 4 cut(s) 17, 43, 689, 916
Fsp4HI GCNGC 2 cut(s) 654, 936
FspBI CTAG 3 cut(s) 120, 284, 539
GluI GCNGC 2 cut(s) 654, 936
HaeIII GGCC 1 cut(s) 90
HapII CCGG 2 cut(s) 273, 464
Hin1II CATG 4 cut(s) 149, 376, 536, 847
HincII GTYRAC 5 cut(s) 525, 561, 823, 982, 1076
HindII GTYRAC 5 cut(s) 525, 561, 823, 982, 1076
HinfI GANTC 6 cut(s) 362, 750, 772, 804, 851, 941
HpaI GTTAAC 2 cut(s) 561, 823
HpaII CCGG 2 cut(s) 273, 464
Hpy166II GTNNAC 5 cut(s) 525, 561, 823, 982, 1076
Hpy188I TCNGA 4 cut(s) 679, 765, 946, 1103
Hpy188III TCNNGA 6 cut(s) 146, 400, 413, 711, 965, 998
Hpy8I GTNNAC 5 cut(s) 525, 561, 823, 982, 1076
HpyCH4III ACNGT 1 cut(s) 1025
HpyCH4IV ACGT 1 cut(s) 920
HpyCH4V TGCA 6 cut(s) 388, 471, 484, 532, 605, 702
HpyF10VI GCNNNNNNNGC 1 cut(s) 468
HpyF3I CTNAG 2 cut(s) 446, 657
HpySE526I ACGT 1 cut(s) 920
Hsp92II CATG 4 cut(s) 149, 376, 536, 847
KspAI GTTAAC 2 cut(s) 561, 823
Kzo9I GATC 5 cut(s) 178, 393, 402, 487, 1005
LmnI GCTCC 1 cut(s) 991
Lsp1109I GCAGC 2 cut(s) 640, 947
LweI GCATC 3 cut(s) 345, 592, 711
MaeI CTAG 3 cut(s) 120, 284, 539
MaeII ACGT 1 cut(s) 920
MaeIII GTNAC 2 cut(s) 424, 683
MalI GATC 5 cut(s) 180, 395, 404, 489, 1007
MboI GATC 5 cut(s) 178, 393, 402, 487, 1005
MboII GAAGA 6 cut(s) 187, 288, 328, 388, 586, 737
MflI RGATCY 2 cut(s) 393, 487
MluCI AATT 3 cut(s) 125, 149, 476
MlyI GAGTC 1 cut(s) 935
MmeI TCCRAC 1 cut(s) 244
Mph1103I ATGCAT 2 cut(s) 607, 704
MseI TTAA 3 cut(s) 210, 560, 822
MspI CCGG 2 cut(s) 273, 464
MspR9I CCNGG 2 cut(s) 273, 464
Mva1269I GAATGC 2 cut(s) 251, 350
MwoI GCNNNNNNNGC 1 cut(s) 468
NciI CCSGG 2 cut(s) 273, 464
NdeII GATC 5 cut(s) 178, 393, 402, 487, 1005
NlaIII CATG 4 cut(s) 149, 376, 536, 847
NlaIV GGNNCC 1 cut(s) 901
NmuCI GTSAC 1 cut(s) 424
NsiI ATGCAT 2 cut(s) 607, 704
NspI RCATGY 1 cut(s) 847
PagI TCATGA 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 639
PctI GAATGC 2 cut(s) 251, 350
PfeI GAWTC 5 cut(s) 362, 750, 772, 804, 851
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 271
PkrI GCNGC 2 cut(s) 655, 937
PleI GAGTC 1 cut(s) 935
PpsI GAGTC 1 cut(s) 935
PspN4I GGNNCC 1 cut(s) 901
PstNI CAGNNNCTG 2 cut(s) 854, 944
PsuI RGATCY 2 cut(s) 393, 487
PsyI GACNNNGTC 1 cut(s) 80
RsaI GTAC 3 cut(s) 166, 543, 743
RsaNI GTAC 3 cut(s) 165, 542, 742
SaqAI TTAA 3 cut(s) 210, 560, 822
SatI GCNGC 2 cut(s) 654, 936
Sau3AI GATC 5 cut(s) 178, 393, 402, 487, 1005
SchI GAGTC 1 cut(s) 935
ScrFI CCNGG 2 cut(s) 273, 464
SfaNI GCATC 3 cut(s) 345, 592, 711
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 3 cut(s) 125, 149, 476
SsiI CCGC 5 cut(s) 251, 438, 580, 739, 933
SspMI CTAG 3 cut(s) 120, 284, 539
StyD4I CCNGG 2 cut(s) 271, 462
TaaI ACNGT 1 cut(s) 1025
TaiI ACGT 1 cut(s) 923
TaqI TCGA 3 cut(s) 369, 807, 1008
TasI AATT 3 cut(s) 125, 149, 476
TatI WGTACW 2 cut(s) 164, 541
TfiI GAWTC 5 cut(s) 362, 750, 772, 804, 851
Tru1I TTAA 3 cut(s) 210, 560, 822
Tru9I TTAA 3 cut(s) 210, 560, 822
TscAI CASTG 1 cut(s) 294
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 2 cut(s) 653, 935
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 5 cut(s) 162, 258, 357, 528, 918
TspRI CASTG 1 cut(s) 294
Tth111I GACNNNGTC 1 cut(s) 80
XagI CCTNNNNNAGG 1 cut(s) 84
XapI RAATTY 2 cut(s) 149, 476
XceI RCATGY 1 cut(s) 847
XspI CTAG 3 cut(s) 120, 284, 539
Zsp2I ATGCAT 2 cut(s) 607, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.