MD15G1124400.v1.1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001445
Physical Location & Seq
Reverse (-)
9037043 .. 9042547
5505 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1124400.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGGGCGAAACAAGGGACGACGTCTACGACGAGGAGCTTGTTGATTACGAAGAAGAGGAGGAGAAGGCCCCTGACTCTGCAGCTAAAGTCAACGGCGAAGCCCCCAAAAAGGGATATGTTGGAATTCACAGTTCAGGGTTCAGAGATTTCCTTTTGAAGCCTGAGCTTCTTCGGTCTATTGTCGACTCTGGATTTGAGCATCCTTCTGAAGTGCAACATGAATGTATCCCTCAAGCTATCCTCGGAATGGATGTTATTTGTCAAGCTAAATCTGGGATGGGAAAAACTGCTGTTTTTGTTCTATCATCTTTACAGCAGATCGACCCTGTCGCAGGCCAAGTCTCTGCCCTTGTCCTTTGCCATACTAGAGAATTAGCATACCAGATTTGTCATGAATTTGAGAGGTTCAGTACATACTTGCCTGATCTCAAGGTTGCTGTCTTCTATGGTGGTGTTAATATCAAACTTCACAAAGATTTACTGAAAAATGAATGCCCGCACATTGTTGTTGGAACTCCTGGAAGAATACTAGCACTGGCAAGAGATAAAGACCTCTCCTTGAAGAATGTGAGGCATTTTATCCTTGATGAATGTGACAAGATGCTTGAATCACTCGACATGAGGAGAGATGTGCAGGAGATCTTCAAGATGACGCCTCACGATAAGCAAGTGATGATGTTTTCCGCAACACTCAGCAAAGACATCCGCCCAGTTTGCAAGAAATTTATGCAAGATCCTATGGAAATATATGTTGATGATGAAGCCAAGTTGACCTTGCATGGTCTAGTGCAGCACTACATCAAGTTAACTGAGGCAGAGAAGAACCGCAAGTTGAATGACCTCCTTGATGCATTGGACTTCAATCAAGTTGTTATATTCGTCAAAAGCGTTAGCAGAGCTGCTGAGCTGAACAAACTACTTTCAGAGTGTAACTTTCCCTCTATATGCATCCATTCTGGAATGAGCCAAGAAGAAAGGTTGAAGCGGTACAAAGGATTCAAGGAGGGTCAGAACAGGATTCTCGTGGCAACTGATTTAGTCGGTAGAGGAATTGACATTGAGCGTGTTAACATTGTTATCAACTACGACATGCCAGATTCTGCTGACACTTATTTACACAGGGTTGGCAGAGCTGGTAGGTTTGGCACCAAAGGTCTTGCAATTACGTTTGTTTCATCCGCAGCAGACTCTGATGTTCTAAATGATGTTCAGGAGAGGTTTGAGGTTGATATAAAGGAGCTTCCTGAACAGATCGATACTGCCACATACATGCCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006974 GO:0007275 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009555 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:1901360 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

427

Amino Acids

48.09

Weight (kDa)

5.29

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 70 - 235 1.8e-40 DEAD/DEAH box helicase
ResIII PF04851 85 - 232 5.3e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 274 - 382 4.6e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 1145
AccI GTMKAC 2 cut(s) 24, 183
AciI CCGC 6 cut(s) 497, 684, 706, 826, 985, 1179
AclWI GGATC 1 cut(s) 728
AcsI RAATTY 3 cut(s) 123, 395, 722
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 2 cut(s) 21, 653
AfaI GTAC 2 cut(s) 412, 989
AfiI CCNNNNNNNGG 4 cut(s) 109, 110, 247, 332
AgsI TTSAA 8 cut(s) 157, 562, 608, 646, 835, 862, 982, 1000
AjnI CCWGG 1 cut(s) 517
AluBI AGCT 9 cut(s) 37, 83, 166, 236, 266, 899, 907, 1133, 1240
AluI AGCT 9 cut(s) 37, 83, 166, 236, 266, 899, 907, 1133, 1240
Alw26I GTCTC 1 cut(s) 346
AlwI GGATC 1 cut(s) 728
AlwNI CAGNNNCTG 2 cut(s) 1100, 1190
AoxI GGCC 2 cut(s) 66, 334
ApeKI GCWGC 4 cut(s) 80, 790, 899, 1181
ApoI RAATTY 3 cut(s) 123, 395, 722
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
AspS9I GGNCC 1 cut(s) 67
BanI GGYRCC 1 cut(s) 1145
BauI CACGAG 1 cut(s) 1022
BbsI GAAGAC 1 cut(s) 433
BbvI GCAGC 4 cut(s) 92, 802, 886, 1193
BccI CCATC 1 cut(s) 271
BceAI ACGGC 1 cut(s) 109
BciT130I CCWGG 1 cut(s) 519
BciVI GTATCC 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 346
BfaI CTAG 3 cut(s) 366, 530, 785
BfmI CTRYAG 1 cut(s) 78
BfuI GTATCC 1 cut(s) 236
BglII AGATCT 1 cut(s) 639
BisI GCNGC 4 cut(s) 81, 791, 900, 1182
BlpI GCTNAGC 1 cut(s) 903
BlsI GCNGC 4 cut(s) 82, 792, 901, 1183
Bme1390I CCNGG 1 cut(s) 519
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 2 cut(s) 69, 1147
BmrFI CCNGG 1 cut(s) 519
BmrI ACTGGG 1 cut(s) 704
BmsI GCATC 4 cut(s) 208, 591, 838, 957
BmuI ACTGGG 1 cut(s) 704
BpiI GAAGAC 1 cut(s) 433
Bpu10I CCTNAGC 1 cut(s) 162
Bpu1102I GCTNAGC 1 cut(s) 903
BpuEI CTTGAG 2 cut(s) 216, 413
Bsa29I ATCGAT 1 cut(s) 1254
BsaHI GRCGYC 2 cut(s) 21, 653
BsaJI CCNNGG 1 cut(s) 241
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 110, 247, 332
Bse1I ACTGG 2 cut(s) 540, 710
BseBI CCWGG 1 cut(s) 519
BseCI ATCGAT 1 cut(s) 1254
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 6 cut(s) 199, 256, 282, 702, 948, 1175
BseLI CCNNNNNNNGG 4 cut(s) 109, 110, 247, 332
BseMII CTCAG 4 cut(s) 153, 706, 801, 894
BseNI ACTGG 2 cut(s) 540, 710
BseRI GAGGAG 4 cut(s) 47, 71, 74, 637
BseXI GCAGC 4 cut(s) 92, 802, 886, 1193
BsgI GTGCAG 2 cut(s) 653, 809
BshFI GGCC 2 cut(s) 68, 336
BshNI GGYRCC 1 cut(s) 1145
BshVI ATCGAT 1 cut(s) 1254
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 4 cut(s) 109, 110, 247, 332
BsmAI GTCTC 1 cut(s) 346
BsmFI GGGAC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 497
BsnI GGCC 2 cut(s) 68, 336
Bsp143I GATC 5 cut(s) 318, 424, 639, 733, 1251
Bsp1720I GCTNAGC 1 cut(s) 903
BspACI CCGC 6 cut(s) 497, 684, 706, 826, 985, 1179
BspANI GGCC 2 cut(s) 68, 336
BspCNI CTCAG 4 cut(s) 154, 705, 802, 895
BspDI ATCGAT 1 cut(s) 1254
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 2 cut(s) 69, 1147
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 1 cut(s) 728
BspT107I GGYRCC 1 cut(s) 1145
BsrI ACTGG 2 cut(s) 540, 710
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 5 cut(s) 318, 424, 639, 733, 1251
BssNI GRCGYC 2 cut(s) 21, 653
BssSI CACGAG 1 cut(s) 1022
Bst2BI CACGAG 1 cut(s) 1022
Bst2UI CCWGG 1 cut(s) 519
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 1 cut(s) 48
BstACI GRCGYC 2 cut(s) 21, 653
BstC8I GCNNGC 2 cut(s) 334, 497
BstDEI CTNAG 4 cut(s) 162, 692, 810, 903
BstENI CCTNNNNNAGG 1 cut(s) 330
BstF5I GGATG 6 cut(s) 199, 256, 282, 702, 948, 1175
BstKTI GATC 5 cut(s) 321, 427, 642, 736, 1254
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 5 cut(s) 318, 424, 639, 733, 1251
BstMWI GCNNNNNNNGC 1 cut(s) 714
BstNI CCWGG 1 cut(s) 519
BstNSI RCATGY 2 cut(s) 1093, 1273
BstSCI CCNGG 1 cut(s) 517
BstSFI CTRYAG 1 cut(s) 78
BstV1I GCAGC 4 cut(s) 92, 802, 886, 1193
BstV2I GAAGAC 1 cut(s) 433
BstX2I RGATCY 2 cut(s) 639, 733
BstYI RGATCY 2 cut(s) 639, 733
Bsu15I ATCGAT 1 cut(s) 1254
BsuI GTATCC 1 cut(s) 236
BsuRI GGCC 2 cut(s) 68, 336
BsuTUI ATCGAT 1 cut(s) 1254
BtsCI GGATG 6 cut(s) 199, 256, 282, 702, 948, 1175
BtsIMutI CAGTG 1 cut(s) 533
Cac8I GCNNGC 2 cut(s) 334, 497
CaiI CAGNNNCTG 2 cut(s) 1100, 1190
CciI TCATGA 1 cut(s) 391
Cfr13I GGNCC 1 cut(s) 67
ClaI ATCGAT 1 cut(s) 1254
CseI GACGC 1 cut(s) 661
Csp6I GTAC 2 cut(s) 411, 988
CviAII CATG 6 cut(s) 218, 392, 619, 779, 1090, 1270
CviQI GTAC 2 cut(s) 411, 988
DdeI CTNAG 4 cut(s) 162, 692, 810, 903
DpnI GATC 5 cut(s) 320, 426, 641, 735, 1253
DpnII GATC 5 cut(s) 318, 424, 639, 733, 1251
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
EciI GGCGGA 1 cut(s) 695
Eco57I CTGAAG 1 cut(s) 228
EcoNI CCTNNNNNAGG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 123
EcoRII CCWGG 1 cut(s) 517
EcoT22I ATGCAT 2 cut(s) 853, 950
FaeI CATG 6 cut(s) 221, 395, 622, 782, 1093, 1273
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FaqI GGGAC 1 cut(s) 29
FatI CATG 6 cut(s) 217, 391, 618, 778, 1089, 1269
FauI CCCGC 1 cut(s) 504
FblI GTMKAC 2 cut(s) 24, 183
Fnu4HI GCNGC 4 cut(s) 81, 791, 900, 1182
FokI GGATG 6 cut(s) 186, 263, 289, 689, 935, 1162
Fsp4HI GCNGC 4 cut(s) 81, 791, 900, 1182
FspBI CTAG 3 cut(s) 366, 530, 785
GluI GCNGC 4 cut(s) 81, 791, 900, 1182
HaeIII GGCC 2 cut(s) 68, 336
HgaI GACGC 1 cut(s) 661
Hin1I GRCGYC 2 cut(s) 21, 653
Hin1II CATG 6 cut(s) 221, 395, 622, 782, 1093, 1273
HincII GTYRAC 5 cut(s) 91, 184, 771, 807, 1069
HindII GTYRAC 5 cut(s) 91, 184, 771, 807, 1069
HinfI GANTC 7 cut(s) 74, 185, 608, 996, 1018, 1097, 1187
HpaI GTTAAC 2 cut(s) 807, 1069
Hpy166II GTNNAC 6 cut(s) 25, 91, 184, 771, 807, 1069
Hpy188I TCNGA 6 cut(s) 143, 208, 245, 925, 1011, 1192
Hpy188III TCNNGA 7 cut(s) 189, 392, 646, 659, 957, 1211, 1244
Hpy8I GTNNAC 6 cut(s) 25, 91, 184, 771, 807, 1069
Hpy99I CGWCG 2 cut(s) 23, 32
HpyAV CCTTC 2 cut(s) 58, 213
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 2 cut(s) 21, 1166
HpyF10VI GCNNNNNNNGC 1 cut(s) 714
HpyF3I CTNAG 4 cut(s) 162, 692, 810, 903
HpySE526I ACGT 2 cut(s) 21, 1166
Hsp92I GRCGYC 2 cut(s) 21, 653
Hsp92II CATG 6 cut(s) 221, 395, 622, 782, 1093, 1273
KspAI GTTAAC 2 cut(s) 807, 1069
Kzo9I GATC 5 cut(s) 318, 424, 639, 733, 1251
LmnI GCTCC 2 cut(s) 34, 1237
Lsp1109I GCAGC 4 cut(s) 92, 802, 886, 1193
LweI GCATC 4 cut(s) 208, 591, 838, 957
MaeI CTAG 3 cut(s) 366, 530, 785
MaeII ACGT 2 cut(s) 21, 1166
MaeIII GTNAC 2 cut(s) 593, 929
MalI GATC 5 cut(s) 320, 426, 641, 735, 1253
MboI GATC 5 cut(s) 318, 424, 639, 733, 1251
MboII GAAGA 9 cut(s) 62, 65, 161, 433, 534, 574, 634, 832, 983
MflI RGATCY 2 cut(s) 639, 733
MluCI AATT 6 cut(s) 123, 371, 395, 722, 1050, 1161
MlyI GAGTC 3 cut(s) 68, 179, 1181
MmeI TCCRAC 2 cut(s) 100, 490
Mph1103I ATGCAT 2 cut(s) 853, 950
MseI TTAA 4 cut(s) 456, 806, 1068, 1279
MslI CAYNNNNRTG 1 cut(s) 1268
MspR9I CCNGG 1 cut(s) 519
Mva1269I GAATGC 1 cut(s) 497
MvaI CCWGG 1 cut(s) 519
MwoI GCNNNNNNNGC 1 cut(s) 714
NdeII GATC 5 cut(s) 318, 424, 639, 733, 1251
NlaIII CATG 6 cut(s) 221, 395, 622, 782, 1093, 1273
NlaIV GGNNCC 2 cut(s) 69, 1147
NmuCI GTSAC 1 cut(s) 593
NsiI ATGCAT 2 cut(s) 853, 950
NspI RCATGY 2 cut(s) 1093, 1273
PagI TCATGA 1 cut(s) 391
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 27, 885
PctI GAATGC 1 cut(s) 497
PfeI GAWTC 4 cut(s) 608, 996, 1018, 1097
PflFI GACNNNGTC 2 cut(s) 20, 326
PfoI TCCNGGA 1 cut(s) 517
PkrI GCNGC 4 cut(s) 82, 792, 901, 1183
PleI GAGTC 3 cut(s) 68, 179, 1181
PpsI GAGTC 3 cut(s) 68, 179, 1181
Psp6I CCWGG 1 cut(s) 517
PspGI CCWGG 1 cut(s) 517
PspN4I GGNNCC 2 cut(s) 69, 1147
PspPI GGNCC 1 cut(s) 67
PstI CTGCAG 1 cut(s) 82
PstNI CAGNNNCTG 2 cut(s) 1100, 1190
PsuI RGATCY 2 cut(s) 639, 733
PsyI GACNNNGTC 2 cut(s) 20, 326
RsaI GTAC 2 cut(s) 412, 989
RsaNI GTAC 2 cut(s) 411, 988
RseI CAYNNNNRTG 1 cut(s) 1268
SalI GTCGAC 1 cut(s) 182
SaqAI TTAA 4 cut(s) 456, 806, 1068, 1279
SatI GCNGC 4 cut(s) 81, 791, 900, 1182
Sau3AI GATC 5 cut(s) 318, 424, 639, 733, 1251
Sau96I GGNCC 1 cut(s) 67
SchI GAGTC 3 cut(s) 68, 179, 1181
ScrFI CCNGG 1 cut(s) 519
SfaNI GCATC 4 cut(s) 208, 591, 838, 957
SfcI CTRYAG 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 1268
SmlI CTYRAG 2 cut(s) 231, 428
SmoI CTYRAG 2 cut(s) 231, 428
Sse9I AATT 6 cut(s) 123, 371, 395, 722, 1050, 1161
SsiI CCGC 6 cut(s) 497, 684, 706, 826, 985, 1179
SspMI CTAG 3 cut(s) 366, 530, 785
StyD4I CCNGG 1 cut(s) 517
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 2 cut(s) 24, 1169
TaqI TCGA 4 cut(s) 183, 321, 615, 1254
TaqII GACCGA 1 cut(s) 162
TasI AATT 6 cut(s) 123, 371, 395, 722, 1050, 1161
TatI WGTACW 1 cut(s) 410
TfiI GAWTC 4 cut(s) 608, 996, 1018, 1097
Tru1I TTAA 4 cut(s) 456, 806, 1068, 1279
Tru9I TTAA 4 cut(s) 456, 806, 1068, 1279
TscAI CASTG 1 cut(s) 540
TseFI GTSAC 1 cut(s) 593
TseI GCWGC 4 cut(s) 80, 790, 899, 1181
Tsp45I GTSAC 1 cut(s) 593
TspDTI ATGAA 6 cut(s) 234, 408, 504, 603, 774, 1164
TspRI CASTG 1 cut(s) 540
Tth111I GACNNNGTC 2 cut(s) 20, 326
XagI CCTNNNNNAGG 1 cut(s) 330
XapI RAATTY 3 cut(s) 123, 395, 722
XceI RCATGY 2 cut(s) 1093, 1273
XmiI GTMKAC 2 cut(s) 24, 183
XspI CTAG 3 cut(s) 366, 530, 785
ZraI GACGTC 1 cut(s) 22
Zsp2I ATGCAT 2 cut(s) 853, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.