Prupe.1G479900_v2.0.a1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001445
Physical Location & Seq
Reverse (-)
40107379 .. 40113148
5770 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G479900.6

Sequence Viewer

Length: 1281 bp
ATGGGCGAAACTAGGGACGATGTGTACGAGGAGGAGCTTGTCGATTATGAAGAAGAGGAGGAGAAGGCCCCCGACTCTGCAGCTAAAGTCAATGGCGAAGCCCCTAAGAAGGGATATGTTGGAATTCACAGTTCAGGGTTCAGAGATTTCCTTTTGAAGCCAGAGCTTCTTCGAGCTATTGTGGACTCCGGGTTTGAGCATCCGTCTGAAGTGCAACATGAATGTATTCCTCAAGCTATCCTGGGAATGGATGTCATTTGTCAAGCTAAATCTGGGATGGGGAAAACTGCTGTTTTTGTTCTATCAACTTTGCAGCAGATTGACCCTGTAGCAGGCCAAGTTTCTGCACTCGTTCTTTGCCATACTAGAGAATTAGCTTACCAGATCTGTCACGAATTTGAGAGGTTCAGTACGTACTTGCCTGATCTCAAGGTTGCTGTCTTCTACGGTGGTGTCAATATCAAACTTCACAAAGATTTGCTGAAAAATGAATGCCCTCACATTGTTGTTGGAACGCCTGGAAGAATATTGGCATTGGCAAGGGATAAAGACCTCTCCTTGAAGAATGTGAGGCATTTCATCCTTGATGAATGTGACAAAATGCTTGAATCCCTAGACATGAGGAGAGACGTGCAAGAGATTTTCAAGATGACGCCGCATGATAAGCAAGTGATGATGTTTTCTGCAACACTCAGCAAAGAAATCCGCCCGGTTTGCAAGAAATTTATGCAAGATCCTATGGAAATATATGTCGACGATGAAGCCAAGTTGACACTGCATGGTCTAGTACAGCACTACATCAAGTTAACCGAGGCAGAGAAAAACCGCAAGTTGAACGACCTCCTTGATGCACTGGACTTCAACCAAGTTGTTATATTCGTCAAGAGCGTGAGCAGAGCCGCTGAATTGAACAAGTTACTTTCAGAGTGTAATTTTCCCTCTATATGCATCCATTCTGGAATGAACCAAGAAGAAAGGTTGAAGCGCTACAAAGGATTCAAGGAGGGTCATAACAGGATTCTTGTGGCCACAGATTTGGTCGGCAGAGGAATCGACATTGAGCGTGTTAACATCGTTATCAACTATGACATGCCAGATTCTGCTGACACTTATTTACACAGGGTTGGCAGAGCTGGCAGGTTTGGCACCAAAGGTCTGGCAATTACATTTGTTTCATCTGCAGCGGACTCTGATGTTCTTAATGATGTTCAGGAAAGGTTTGAGGTTGATATAAAGGAGCTTCCTGAACAGATTGATACTGCCACATACATGCCGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006974 GO:0007275 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009555 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:1901360 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

427

Amino Acids

48.15

Weight (kDa)

5.35

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1128
AccB1I GGYRCC 1 cut(s) 1145
AccI GTMKAC 1 cut(s) 753
AciI CCGC 5 cut(s) 656, 706, 826, 900, 1184
AclWI GGATC 1 cut(s) 728
AcoI YGGCCR 1 cut(s) 1026
AcsI RAATTY 3 cut(s) 123, 395, 722
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 653
AfaI GTAC 4 cut(s) 26, 412, 416, 789
AfeI AGCGCT 1 cut(s) 986
AfiI CCNNNNNNNGG 4 cut(s) 109, 110, 247, 332
AgsI TTSAA 9 cut(s) 157, 562, 608, 646, 835, 862, 910, 982, 1000
AjiI CACGTC 1 cut(s) 631
AjnI CCWGG 2 cut(s) 240, 517
AluBI AGCT 9 cut(s) 37, 83, 166, 176, 236, 266, 377, 1133, 1240
AluI AGCT 9 cut(s) 37, 83, 166, 176, 236, 266, 377, 1133, 1240
Alw26I GTCTC 1 cut(s) 621
AlwI GGATC 1 cut(s) 728
AlwNI CAGNNNCTG 1 cut(s) 1100
Aor51HI AGCGCT 1 cut(s) 986
AoxI GGCC 3 cut(s) 66, 334, 1026
ApeKI GCWGC 3 cut(s) 80, 313, 1181
ApoI RAATTY 3 cut(s) 123, 395, 722
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
Asp700I GAANNNNTTC 1 cut(s) 225
AspLEI GCGC 1 cut(s) 987
AspS9I GGNCC 1 cut(s) 67
AsuC2I CCSGG 2 cut(s) 190, 710
BalI TGGCCA 1 cut(s) 1028
BanI GGYRCC 1 cut(s) 1145
BbsI GAAGAC 1 cut(s) 433
BbvI GCAGC 3 cut(s) 92, 325, 1193
BccI CCATC 1 cut(s) 271
BceAI ACGGC 1 cut(s) 1258
BcgI CGANNNNNNTGC 2 cut(s) 1033, 1067
BciT130I CCWGG 2 cut(s) 242, 519
BcnI CCSGG 2 cut(s) 190, 710
BcoDI GTCTC 1 cut(s) 621
BfaI CTAG 4 cut(s) 12, 366, 614, 785
BfmI CTRYAG 3 cut(s) 78, 327, 1179
BfoI RGCGCY 1 cut(s) 988
BfuAI ACCTGC 1 cut(s) 1128
BglII AGATCT 1 cut(s) 384
BisI GCNGC 5 cut(s) 81, 314, 656, 900, 1182
BlsI GCNGC 5 cut(s) 82, 315, 657, 901, 1183
Bme1390I CCNGG 4 cut(s) 190, 242, 519, 710
BmgBI CACGTC 1 cut(s) 631
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 2 cut(s) 69, 1147
BmrFI CCNGG 4 cut(s) 190, 242, 519, 710
BmsI GCATC 3 cut(s) 208, 838, 957
BpiI GAAGAC 1 cut(s) 433
BpuEI CTTGAG 2 cut(s) 216, 413
BpuMI CCSGG 2 cut(s) 190, 710
BsaAI YACGTR 1 cut(s) 414
BsaHI GRCGYC 1 cut(s) 653
BsaJI CCNNGG 2 cut(s) 241, 810
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 110, 247, 332
Bse1I ACTGG 1 cut(s) 858
BseBI CCWGG 2 cut(s) 242, 519
BseDI CCNNGG 2 cut(s) 241, 810
BseGI GGATG 5 cut(s) 199, 256, 282, 579, 948
BseLI CCNNNNNNNGG 4 cut(s) 109, 110, 247, 332
BseMII CTCAG 1 cut(s) 706
BseNI ACTGG 1 cut(s) 858
BseRI GAGGAG 5 cut(s) 44, 47, 71, 74, 637
BseXI GCAGC 3 cut(s) 92, 325, 1193
BsgI GTGCAG 1 cut(s) 330
BshFI GGCC 3 cut(s) 68, 336, 1028
BshNI GGYRCC 1 cut(s) 1145
BsiSI CCGG 2 cut(s) 189, 710
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 4 cut(s) 109, 110, 247, 332
BsmAI GTCTC 1 cut(s) 621
BsmBI CGTCTC 1 cut(s) 621
BsmFI GGGAC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 497
BsnI GGCC 3 cut(s) 68, 336, 1028
Bsp143I GATC 3 cut(s) 384, 424, 733
BspACI CCGC 5 cut(s) 656, 706, 826, 900, 1184
BspANI GGCC 3 cut(s) 68, 336, 1028
BspCNI CTCAG 1 cut(s) 705
BspLI GGNNCC 2 cut(s) 69, 1147
BspMAI CTGCAG 2 cut(s) 82, 1183
BspMI ACCTGC 1 cut(s) 1128
BspPI GGATC 1 cut(s) 728
BspT107I GGYRCC 1 cut(s) 1145
BsrI ACTGG 1 cut(s) 858
BssECI CCNNGG 2 cut(s) 241, 810
BssMI GATC 3 cut(s) 384, 424, 733
BssNI GRCGYC 1 cut(s) 653
Bst2UI CCWGG 2 cut(s) 242, 519
Bst4CI ACNGT 2 cut(s) 131, 449
Bst6I CTCTTC 1 cut(s) 48
BstACI GRCGYC 1 cut(s) 653
BstBAI YACGTR 1 cut(s) 414
BstC8I GCNNGC 2 cut(s) 334, 1135
BstDEI CTNAG 2 cut(s) 105, 692
BstENI CCTNNNNNAGG 1 cut(s) 330
BstF5I GGATG 5 cut(s) 199, 256, 282, 579, 948
BstH2I RGCGCY 1 cut(s) 988
BstHHI GCGC 1 cut(s) 987
BstKTI GATC 3 cut(s) 387, 427, 736
BstMAI GTCTC 1 cut(s) 621
BstMBI GATC 3 cut(s) 384, 424, 733
BstMWI GCNNNNNNNGC 4 cut(s) 664, 714, 1134, 1143
BstNI CCWGG 2 cut(s) 242, 519
BstNSI RCATGY 2 cut(s) 1093, 1273
BstSCI CCNGG 4 cut(s) 188, 240, 517, 708
BstSFI CTRYAG 3 cut(s) 78, 327, 1179
BstSNI TACGTA 1 cut(s) 414
BstV1I GCAGC 3 cut(s) 92, 325, 1193
BstV2I GAAGAC 1 cut(s) 433
BstX2I RGATCY 2 cut(s) 384, 733
BstXI CCANNNNNNTGG 2 cut(s) 1036, 1156
BstYI RGATCY 2 cut(s) 384, 733
BsuRI GGCC 3 cut(s) 68, 336, 1028
BtrI CACGTC 1 cut(s) 631
BtsCI GGATG 5 cut(s) 199, 256, 282, 579, 948
BtsI GCAGTG 1 cut(s) 773
BtsIMutI CAGTG 2 cut(s) 773, 851
BveI ACCTGC 1 cut(s) 1128
Cac8I GCNNGC 2 cut(s) 334, 1135
CaiI CAGNNNCTG 1 cut(s) 1100
CfoI GCGC 1 cut(s) 987
Cfr13I GGNCC 1 cut(s) 67
CseI GACGC 1 cut(s) 661
Csp6I GTAC 4 cut(s) 25, 411, 415, 788
CviAII CATG 6 cut(s) 218, 619, 659, 779, 1090, 1270
CviQI GTAC 4 cut(s) 25, 411, 415, 788
DdeI CTNAG 2 cut(s) 105, 692
DpnI GATC 3 cut(s) 386, 426, 735
DpnII GATC 3 cut(s) 384, 424, 733
EaeI YGGCCR 1 cut(s) 1026
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
EciI GGCGGA 1 cut(s) 695
Eco105I TACGTA 1 cut(s) 414
Eco47III AGCGCT 1 cut(s) 986
Eco57I CTGAAG 1 cut(s) 228
EcoNI CCTNNNNNAGG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 123
EcoRII CCWGG 2 cut(s) 240, 517
EcoT22I ATGCAT 1 cut(s) 950
Esp3I CGTCTC 1 cut(s) 621
FaeI CATG 6 cut(s) 221, 622, 662, 782, 1093, 1273
FaqI GGGAC 1 cut(s) 29
FatI CATG 6 cut(s) 217, 618, 658, 778, 1089, 1269
FblI GTMKAC 1 cut(s) 753
Fnu4HI GCNGC 5 cut(s) 81, 314, 656, 900, 1182
FokI GGATG 5 cut(s) 186, 263, 289, 566, 935
Fsp4HI GCNGC 5 cut(s) 81, 314, 656, 900, 1182
FspBI CTAG 4 cut(s) 12, 366, 614, 785
GlaI GCGC 1 cut(s) 986
GluI GCNGC 5 cut(s) 81, 314, 656, 900, 1182
HaeII RGCGCY 1 cut(s) 988
HaeIII GGCC 3 cut(s) 68, 336, 1028
HapII CCGG 2 cut(s) 189, 710
HgaI GACGC 1 cut(s) 661
HhaI GCGC 1 cut(s) 987
Hin1I GRCGYC 1 cut(s) 653
Hin1II CATG 6 cut(s) 221, 622, 662, 782, 1093, 1273
Hin6I GCGC 1 cut(s) 985
HinP1I GCGC 1 cut(s) 985
HincII GTYRAC 4 cut(s) 754, 771, 807, 1069
HindII GTYRAC 4 cut(s) 754, 771, 807, 1069
HinfI GANTC 8 cut(s) 74, 185, 608, 996, 1018, 1050, 1097, 1187
HpaI GTTAAC 2 cut(s) 807, 1069
HpaII CCGG 2 cut(s) 189, 710
Hpy166II GTNNAC 6 cut(s) 25, 184, 754, 771, 807, 1069
Hpy188I TCNGA 4 cut(s) 143, 208, 925, 1192
Hpy188III TCNNGA 6 cut(s) 392, 646, 883, 957, 1211, 1244
Hpy8I GTNNAC 6 cut(s) 25, 184, 754, 771, 807, 1069
Hpy99I CGWCG 1 cut(s) 758
HpyAV CCTTC 2 cut(s) 58, 103
HpyCH4III ACNGT 2 cut(s) 131, 449
HpyCH4IV ACGT 2 cut(s) 413, 630
HpyF10VI GCNNNNNNNGC 4 cut(s) 664, 714, 1134, 1143
HpyF3I CTNAG 2 cut(s) 105, 692
HpySE526I ACGT 2 cut(s) 413, 630
Hsp92I GRCGYC 1 cut(s) 653
Hsp92II CATG 6 cut(s) 221, 622, 662, 782, 1093, 1273
HspAI GCGC 1 cut(s) 985
KspAI GTTAAC 2 cut(s) 807, 1069
Kzo9I GATC 3 cut(s) 384, 424, 733
LmnI GCTCC 2 cut(s) 34, 1237
Lsp1109I GCAGC 3 cut(s) 92, 325, 1193
LweI GCATC 3 cut(s) 208, 838, 957
MaeI CTAG 4 cut(s) 12, 366, 614, 785
MaeII ACGT 2 cut(s) 413, 630
MaeIII GTNAC 3 cut(s) 389, 593, 915
MalI GATC 3 cut(s) 386, 426, 735
MboI GATC 3 cut(s) 384, 424, 733
MboII GAAGA 7 cut(s) 62, 65, 161, 433, 534, 574, 983
MflI RGATCY 2 cut(s) 384, 733
MlsI TGGCCA 1 cut(s) 1028
MluCI AATT 7 cut(s) 123, 371, 395, 722, 905, 931, 1161
MluNI TGGCCA 1 cut(s) 1028
MlyI GAGTC 3 cut(s) 68, 179, 1181
MmeI TCCRAC 2 cut(s) 100, 490
Mox20I TGGCCA 1 cut(s) 1028
Mph1103I ATGCAT 1 cut(s) 950
MroXI GAANNNNTTC 1 cut(s) 225
MscI TGGCCA 1 cut(s) 1028
MseI TTAA 4 cut(s) 806, 1068, 1200, 1279
MslI CAYNNNNRTG 1 cut(s) 1268
Msp20I TGGCCA 1 cut(s) 1028
MspA1I CMGCKG 2 cut(s) 902, 1184
MspI CCGG 2 cut(s) 189, 710
MspR9I CCNGG 4 cut(s) 190, 242, 519, 710
Mva1269I GAATGC 1 cut(s) 497
MvaI CCWGG 2 cut(s) 242, 519
MwoI GCNNNNNNNGC 4 cut(s) 664, 714, 1134, 1143
NciI CCSGG 2 cut(s) 190, 710
NdeII GATC 3 cut(s) 384, 424, 733
NlaIII CATG 6 cut(s) 221, 622, 662, 782, 1093, 1273
NlaIV GGNNCC 2 cut(s) 69, 1147
NmuCI GTSAC 2 cut(s) 389, 593
NsiI ATGCAT 1 cut(s) 950
NspI RCATGY 2 cut(s) 1093, 1273
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 885
PctI GAATGC 1 cut(s) 497
PdmI GAANNNNTTC 1 cut(s) 225
PfeI GAWTC 5 cut(s) 608, 996, 1018, 1050, 1097
PkrI GCNGC 5 cut(s) 82, 315, 657, 901, 1183
PleI GAGTC 3 cut(s) 68, 179, 1181
PpsI GAGTC 3 cut(s) 68, 179, 1181
Ppu21I YACGTR 1 cut(s) 414
Psp6I CCWGG 2 cut(s) 240, 517
PspGI CCWGG 2 cut(s) 240, 517
PspN4I GGNNCC 2 cut(s) 69, 1147
PspPI GGNCC 1 cut(s) 67
PstI CTGCAG 2 cut(s) 82, 1183
PstNI CAGNNNCTG 1 cut(s) 1100
PsuI RGATCY 2 cut(s) 384, 733
RsaI GTAC 4 cut(s) 26, 412, 416, 789
RsaNI GTAC 4 cut(s) 25, 411, 415, 788
RseI CAYNNNNRTG 1 cut(s) 1268
SalI GTCGAC 1 cut(s) 752
SaqAI TTAA 4 cut(s) 806, 1068, 1200, 1279
SatI GCNGC 5 cut(s) 81, 314, 656, 900, 1182
Sau3AI GATC 3 cut(s) 384, 424, 733
Sau96I GGNCC 1 cut(s) 67
SchI GAGTC 3 cut(s) 68, 179, 1181
ScrFI CCNGG 4 cut(s) 190, 242, 519, 710
SfaNI GCATC 3 cut(s) 208, 838, 957
SfcI CTRYAG 3 cut(s) 78, 327, 1179
SmiMI CAYNNNNRTG 1 cut(s) 1268
SmlI CTYRAG 2 cut(s) 231, 428
SmoI CTYRAG 2 cut(s) 231, 428
SnaBI TACGTA 1 cut(s) 414
Sse9I AATT 7 cut(s) 123, 371, 395, 722, 905, 931, 1161
SsiI CCGC 5 cut(s) 656, 706, 826, 900, 1184
SspI AATATT 1 cut(s) 528
SspMI CTAG 4 cut(s) 12, 366, 614, 785
StyD4I CCNGG 4 cut(s) 188, 240, 517, 708
TaaI ACNGT 2 cut(s) 131, 449
TaiI ACGT 2 cut(s) 416, 633
TaqI TCGA 4 cut(s) 42, 172, 753, 1053
TasI AATT 7 cut(s) 123, 371, 395, 722, 905, 931, 1161
TatI WGTACW 1 cut(s) 787
TauI GCSGC 2 cut(s) 658, 902
TfiI GAWTC 5 cut(s) 608, 996, 1018, 1050, 1097
Tru1I TTAA 4 cut(s) 806, 1068, 1200, 1279
Tru9I TTAA 4 cut(s) 806, 1068, 1200, 1279
TscAI CASTG 2 cut(s) 780, 858
TseFI GTSAC 2 cut(s) 389, 593
TseI GCWGC 3 cut(s) 80, 313, 1181
Tsp45I GTSAC 2 cut(s) 389, 593
TspDTI ATGAA 8 cut(s) 63, 234, 504, 568, 603, 774, 977, 1164
TspGWI ACGGA 1 cut(s) 192
TspRI CASTG 2 cut(s) 780, 858
XagI CCTNNNNNAGG 1 cut(s) 330
XapI RAATTY 3 cut(s) 123, 395, 722
XceI RCATGY 2 cut(s) 1093, 1273
XmiI GTMKAC 1 cut(s) 753
XmnI GAANNNNTTC 1 cut(s) 225
XspI CTAG 4 cut(s) 12, 366, 614, 785
Zsp2I ATGCAT 1 cut(s) 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.