RLG00000011538

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001445
Physical Location & Seq
Reverse (-)
10379926 .. 10384022
4097 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011538

Sequence Viewer

Length: 1188 bp
ATGGGCGAAACAAGGGAAGATGTCTACGACGAAGAGCTCTATGTGTTTTTTGGATGTGTCGCTTCCCATTGCTTTGATTTTTCACTCTTCTTCTATTCCTGCTTGCTTAATTTAATGTTACAACATGAATGCATCCCACAAGCTATTCTGGGAATGGATGTTATCTGCCAAGCTAAATCTGGGATGGGGAAAACGGCTGTATTTGTCCTATCTACTTTGCAGCAGATTGACCCTGTTGCAGGCCAAGTGTCTGCACTTATTCTTTGCCATACTCGAGAATTAGCTTACCAGATTTGCCATGAGTTTGAGAGATTCAGTACCTACTTGCCAGATCTGAAGGTTGCTGTCTTCTATGGTGGTGTCACTATCAAAAATCACAAAGATTTACTTAAAAATGAATGCCCCCACATTGTTGTTGGAACTCCTGGAAGAATACTAGCACTTGCAAGAGATAAAGACCTCTCTTTGAAGAATGTGAGGCATTTTATCCTCGATGAATGTGACAAGATGCTTGAGTCACTTGACATGAGGAGAGATGTGCAAGAGATTTTCAAGATGACGCCACATGATAAGCAAGTAATGATGTTCTCTGCAACACTCAGCAAAGATATCCGACCGGTTTGCAAGAAATTTATGCAAGATCCTATGGAAATATATGTTGATGACGAAGCCAAGTTGACTCTTCATGGTCTAGTACAGCACTACATCAAGCTAACTGAGGCAGAGAAAAATCGCAAGCTGAATGATCTCCTTGATGCACTGGACTTCAACCAGGTTGTCATCTTCGTCAAAAGTGTGAGCAGAGCTGCAGAGTTGAACAAGTTACTCTCAGAGTGTAATTTTCCCTCTATATGCATCCATTCTGGAATGAACCAAGAAGAAAGGTTGAAGCGCTACAAAGGTTTCAAGGAGGGTCAGAACAGGATTCTAGTGGCCACCGATCTGGTTGGTAGAGGAATTGATATTGAACGTGTCAATATCGTTATCAACTATGACATGCCAGACTCTGCGGACACCTACCTGCACAGGGTTGGCAGAGCTGGTAGGTTTGGCACCAAAGGGCTGGCAATTACATTTGTATCATCTGCTTCGGACTCTCAAGTCCTTAATGATGTTCAGGAGAGGTTTGAGGTTGACATAAAGGAGCTTCCTGAACAGATTGATACTGCCACATACATGCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

396

Amino Acids

44.96

Weight (kDa)

5.6

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 41 - 204 2e-40 DEAD/DEAH box helicase
ResIII PF04851 55 - 201 2.2e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 243 - 351 5.9e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1100
Acc36I ACCTGC 1 cut(s) 1029
AccB1I GGYRCC 1 cut(s) 1052
AccI GTMKAC 1 cut(s) 24
AciI CCGC 1 cut(s) 1010
AclWI GGATC 1 cut(s) 635
AcoI YGGCCR 1 cut(s) 933
AcsI RAATTY 1 cut(s) 629
AcuI CTGAAG 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 560
AfaI GTAC 2 cut(s) 319, 696
AfeI AGCGCT 1 cut(s) 893
AfiI CCNNNNNNNGG 2 cut(s) 239, 1027
AflIII ACRYGT 1 cut(s) 970
AgeI ACCGGT 1 cut(s) 616
AgsI TTSAA 7 cut(s) 469, 553, 769, 817, 889, 907, 968
AjnI CCWGG 2 cut(s) 424, 771
AluBI AGCT 9 cut(s) 37, 143, 173, 284, 712, 739, 806, 1040, 1147
AluI AGCT 9 cut(s) 37, 143, 173, 284, 712, 739, 806, 1040, 1147
Alw21I GWGCWC 1 cut(s) 39
AlwI GGATC 1 cut(s) 635
AlwNI CAGNNNCTG 1 cut(s) 1007
Ama87I CYCGRG 1 cut(s) 273
Aor51HI AGCGCT 1 cut(s) 893
AoxI GGCC 2 cut(s) 241, 933
ApeKI GCWGC 2 cut(s) 220, 806
ApoI RAATTY 1 cut(s) 629
AsiGI ACCGGT 1 cut(s) 616
AspLEI GCGC 1 cut(s) 894
AvaI CYCGRG 1 cut(s) 273
BaeI ACNNNNGTAYC 2 cut(s) 1062, 1095
BalI TGGCCA 1 cut(s) 935
BanI GGYRCC 1 cut(s) 1052
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 340
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 2 cut(s) 232, 793
BccI CCATC 1 cut(s) 178
BceAI ACGGC 1 cut(s) 210
BcgI CGANNNNNNTGC 2 cut(s) 603, 637
BciT130I CCWGG 2 cut(s) 426, 773
BfaI CTAG 3 cut(s) 437, 692, 929
BfmI CTRYAG 1 cut(s) 807
BfoI RGCGCY 1 cut(s) 895
BfuAI ACCTGC 1 cut(s) 1029
BglII AGATCT 1 cut(s) 331
BisI GCNGC 2 cut(s) 221, 807
BlsI GCNGC 2 cut(s) 222, 808
Bme1390I CCNGG 2 cut(s) 426, 773
BmeT110I CYCGRG 1 cut(s) 273
BmiI GGNNCC 1 cut(s) 1054
BmrFI CCNGG 2 cut(s) 426, 773
BmsI GCATC 4 cut(s) 141, 498, 745, 864
BpiI GAAGAC 1 cut(s) 340
BpuEI CTTGAG 2 cut(s) 533, 1083
BsaHI GRCGYC 1 cut(s) 560
BsaWI WCCGGW 1 cut(s) 616
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 1027
Bse118I RCCGGY 1 cut(s) 616
Bse1I ACTGG 1 cut(s) 765
Bse3DI GCAATG 1 cut(s) 67
BseBI CCWGG 2 cut(s) 426, 773
BseGI GGATG 5 cut(s) 59, 132, 163, 189, 855
BseLI CCNNNNNNNGG 2 cut(s) 239, 1027
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 3 cut(s) 613, 708, 843
BseNI ACTGG 1 cut(s) 765
BseRI GAGGAG 1 cut(s) 544
BseXI GCAGC 2 cut(s) 232, 793
BsgI GTGCAG 2 cut(s) 237, 1007
Bsh1285I CGRYCG 1 cut(s) 617
BshFI GGCC 2 cut(s) 243, 935
BshNI GGYRCC 1 cut(s) 1052
BshTI ACCGGT 1 cut(s) 616
BsiEI CGRYCG 1 cut(s) 617
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 1 cut(s) 617
BslI CCNNNNNNNGG 2 cut(s) 239, 1027
BsmI GAATGC 2 cut(s) 134, 404
BsnI GGCC 2 cut(s) 243, 935
BsoBI CYCGRG 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 4 cut(s) 331, 640, 745, 940
BspACI CCGC 1 cut(s) 1010
BspANI GGCC 2 cut(s) 243, 935
BspCNI CTCAG 3 cut(s) 612, 709, 842
BspLI GGNNCC 1 cut(s) 1054
BspMAI CTGCAG 1 cut(s) 811
BspMI ACCTGC 1 cut(s) 1029
BspPI GGATC 1 cut(s) 635
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 1052
BsrDI GCAATG 1 cut(s) 67
BsrFI RCCGGY 1 cut(s) 616
BsrI ACTGG 1 cut(s) 765
BssAI RCCGGY 1 cut(s) 616
BssMI GATC 4 cut(s) 331, 640, 745, 940
BssNI GRCGYC 1 cut(s) 560
Bst2UI CCWGG 2 cut(s) 426, 773
Bst6I CTCTTC 3 cut(s) 27, 92, 687
BstACI GRCGYC 1 cut(s) 560
BstC8I GCNNGC 4 cut(s) 104, 241, 737, 1065
BstDEI CTNAG 3 cut(s) 599, 717, 829
BstENI CCTNNNNNAGG 1 cut(s) 237
BstF5I GGATG 5 cut(s) 59, 132, 163, 189, 855
BstH2I RGCGCY 1 cut(s) 895
BstHHI GCGC 1 cut(s) 894
BstKTI GATC 4 cut(s) 334, 643, 748, 943
BstMBI GATC 4 cut(s) 331, 640, 745, 940
BstMCI CGRYCG 1 cut(s) 617
BstNI CCWGG 2 cut(s) 426, 773
BstNSI RCATGY 2 cut(s) 1000, 1180
BstSCI CCNGG 2 cut(s) 424, 771
BstSFI CTRYAG 1 cut(s) 807
BstV1I GCAGC 2 cut(s) 232, 793
BstV2I GAAGAC 1 cut(s) 340
BstX2I RGATCY 2 cut(s) 331, 640
BstXI CCANNNNNNTGG 2 cut(s) 943, 1063
BstYI RGATCY 2 cut(s) 331, 640
BsuRI GGCC 2 cut(s) 243, 935
BtsCI GGATG 5 cut(s) 59, 132, 163, 189, 855
BtsIMutI CAGTG 1 cut(s) 758
BveI ACCTGC 1 cut(s) 1029
Cac8I GCNNGC 4 cut(s) 104, 241, 737, 1065
CaiI CAGNNNCTG 1 cut(s) 1007
CfoI GCGC 1 cut(s) 894
Cfr10I RCCGGY 1 cut(s) 616
CseI GACGC 1 cut(s) 568
CsiI ACCWGGT 1 cut(s) 771
Csp6I GTAC 2 cut(s) 318, 695
CspAI ACCGGT 1 cut(s) 616
CviAII CATG 7 cut(s) 125, 299, 526, 566, 686, 997, 1177
CviQI GTAC 2 cut(s) 318, 695
DdeI CTNAG 3 cut(s) 599, 717, 829
DpnI GATC 4 cut(s) 333, 642, 747, 942
DpnII GATC 4 cut(s) 331, 640, 745, 940
DrdI GACNNNNNNGTC 1 cut(s) 1100
DseDI GACNNNNNNGTC 1 cut(s) 1100
EaeI YGGCCR 1 cut(s) 933
Eam1104I CTCTTC 3 cut(s) 27, 92, 687
EarI CTCTTC 3 cut(s) 27, 92, 687
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 39
Eco32I GATATC 1 cut(s) 610
Eco47III AGCGCT 1 cut(s) 893
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 356
Eco88I CYCGRG 1 cut(s) 273
EcoICRI GAGCTC 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 237
EcoRII CCWGG 2 cut(s) 424, 771
EcoRV GATATC 1 cut(s) 610
EcoT22I ATGCAT 2 cut(s) 134, 857
EcoT38I GRGCYC 1 cut(s) 39
FaeI CATG 7 cut(s) 128, 302, 529, 569, 689, 1000, 1180
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FatI CATG 7 cut(s) 124, 298, 525, 565, 685, 996, 1176
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 221, 807
FokI GGATG 5 cut(s) 66, 119, 170, 196, 842
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 2 cut(s) 221, 807
FspBI CTAG 3 cut(s) 437, 692, 929
GlaI GCGC 1 cut(s) 893
GluI GCNGC 2 cut(s) 221, 807
HaeII RGCGCY 1 cut(s) 895
HaeIII GGCC 2 cut(s) 243, 935
HapII CCGG 1 cut(s) 617
HgaI GACGC 1 cut(s) 568
HhaI GCGC 1 cut(s) 894
Hin1I GRCGYC 1 cut(s) 560
Hin1II CATG 7 cut(s) 128, 302, 529, 569, 689, 1000, 1180
Hin6I GCGC 1 cut(s) 892
HinP1I GCGC 1 cut(s) 892
HincII GTYRAC 2 cut(s) 678, 1135
HindII GTYRAC 2 cut(s) 678, 1135
HinfI GANTC 6 cut(s) 312, 515, 679, 925, 1004, 1094
HpaII CCGG 1 cut(s) 617
Hpy166II GTNNAC 3 cut(s) 25, 678, 1135
Hpy188I TCNGA 5 cut(s) 336, 614, 832, 918, 1093
Hpy188III TCNNGA 6 cut(s) 275, 553, 864, 1118, 1151, 1185
Hpy8I GTNNAC 3 cut(s) 25, 678, 1135
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 1 cut(s) 331
HpyCH4IV ACGT 1 cut(s) 970
HpyF3I CTNAG 3 cut(s) 599, 717, 829
HpySE526I ACGT 1 cut(s) 970
Hsp92I GRCGYC 1 cut(s) 560
Hsp92II CATG 7 cut(s) 128, 302, 529, 569, 689, 1000, 1180
HspAI GCGC 1 cut(s) 892
Kzo9I GATC 4 cut(s) 331, 640, 745, 940
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 1 cut(s) 1144
Lsp1109I GCAGC 2 cut(s) 232, 793
LweI GCATC 4 cut(s) 141, 498, 745, 864
MabI ACCWGGT 1 cut(s) 771
MaeI CTAG 3 cut(s) 437, 692, 929
MaeII ACGT 1 cut(s) 970
MaeIII GTNAC 5 cut(s) 117, 361, 500, 516, 822
MalI GATC 4 cut(s) 333, 642, 747, 942
MboI GATC 4 cut(s) 331, 640, 745, 940
MflI RGATCY 2 cut(s) 331, 640
MhlI GDGCHC 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 935
MluCI AATT 6 cut(s) 109, 278, 629, 838, 957, 1068
MluNI TGGCCA 1 cut(s) 935
MlyI GAGTC 4 cut(s) 524, 673, 998, 1088
MmeI TCCRAC 2 cut(s) 397, 637
Mox20I TGGCCA 1 cut(s) 935
Mph1103I ATGCAT 2 cut(s) 134, 857
MscI TGGCCA 1 cut(s) 935
MseI TTAA 4 cut(s) 108, 113, 390, 1107
MslI CAYNNNNRTG 1 cut(s) 1175
Msp20I TGGCCA 1 cut(s) 935
MspI CCGG 1 cut(s) 617
MspR9I CCNGG 2 cut(s) 426, 773
Mva1269I GAATGC 2 cut(s) 134, 404
MvaI CCWGG 2 cut(s) 426, 773
NdeII GATC 4 cut(s) 331, 640, 745, 940
NlaIII CATG 7 cut(s) 128, 302, 529, 569, 689, 1000, 1180
NlaIV GGNNCC 1 cut(s) 1054
NmuCI GTSAC 3 cut(s) 361, 500, 516
NsiI ATGCAT 2 cut(s) 134, 857
NspI RCATGY 2 cut(s) 1000, 1180
PaeR7I CTCGAG 1 cut(s) 273
PciSI GCTCTTC 1 cut(s) 27
PctI GAATGC 2 cut(s) 134, 404
PfeI GAWTC 2 cut(s) 312, 925
PfoI TCCNGGA 1 cut(s) 424
PinAI ACCGGT 1 cut(s) 616
PkrI GCNGC 2 cut(s) 222, 808
PleI GAGTC 4 cut(s) 523, 673, 998, 1088
PpsI GAGTC 4 cut(s) 523, 673, 998, 1088
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 2 cut(s) 424, 771
PspGI CCWGG 2 cut(s) 424, 771
PspN4I GGNNCC 1 cut(s) 1054
PstI CTGCAG 1 cut(s) 811
PstNI CAGNNNCTG 1 cut(s) 1007
PsuI RGATCY 2 cut(s) 331, 640
RsaI GTAC 2 cut(s) 319, 696
RsaNI GTAC 2 cut(s) 318, 695
RseI CAYNNNNRTG 1 cut(s) 1175
SacI GAGCTC 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 4 cut(s) 108, 113, 390, 1107
SatI GCNGC 2 cut(s) 221, 807
Sau3AI GATC 4 cut(s) 331, 640, 745, 940
SchI GAGTC 4 cut(s) 524, 673, 998, 1088
ScrFI CCNGG 2 cut(s) 426, 773
SduI GDGCHC 1 cut(s) 39
SexAI ACCWGGT 1 cut(s) 771
SfaNI GCATC 4 cut(s) 141, 498, 745, 864
SfcI CTRYAG 1 cut(s) 807
Sfr274I CTCGAG 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 1175
SmlI CTYRAG 3 cut(s) 273, 512, 1098
SmoI CTYRAG 3 cut(s) 273, 512, 1098
Sse9I AATT 6 cut(s) 109, 278, 629, 838, 957, 1068
SsiI CCGC 1 cut(s) 1010
SspMI CTAG 3 cut(s) 437, 692, 929
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 424, 771
TaiI ACGT 1 cut(s) 973
TaqI TCGA 2 cut(s) 274, 492
TasI AATT 6 cut(s) 109, 278, 629, 838, 957, 1068
TatI WGTACW 1 cut(s) 694
TfiI GAWTC 2 cut(s) 312, 925
Tru1I TTAA 4 cut(s) 108, 113, 390, 1107
Tru9I TTAA 4 cut(s) 108, 113, 390, 1107
TscAI CASTG 1 cut(s) 765
TseFI GTSAC 3 cut(s) 361, 500, 516
TseI GCWGC 2 cut(s) 220, 806
Tsp45I GTSAC 3 cut(s) 361, 500, 516
TspDTI ATGAA 5 cut(s) 141, 411, 510, 674, 884
TspRI CASTG 1 cut(s) 765
XagI CCTNNNNNAGG 1 cut(s) 237
XapI RAATTY 1 cut(s) 629
XceI RCATGY 2 cut(s) 1000, 1180
XcmI CCANNNNNNNNNTGG 2 cut(s) 176, 413
XhoI CTCGAG 1 cut(s) 273
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 3 cut(s) 437, 692, 929
Zsp2I ATGCAT 2 cut(s) 134, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.