Rh2AG379000

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001445
Physical Location & Seq
Forward (+)
56762836 .. 56766826
3991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG379000.1

Sequence Viewer

Length: 1035 bp
ATGGATGTTCTTTGCCAAGCAAAATCTGGGATGGGCAAAACTGCAGTCTTTGTTCTGTCAACTCTTCAGCAAATAGATCCGGTTGCTGGTCAAGTTGCTGCTCTCATTCTATGCCACACAAGAGAATTGGCTTACCAGATTTGTCATGAATTTGAGAGATTCAGTACGTATTTGCCCGAGTTAAAAGTTGCGGTTTTCTATGGAGGTGTAACTATTAAGGTTCACAGAGATTTGCTGAAAAATGAATGCCCTCATATTGTTGTTGGAACACCTGGGAGAGTGTTGGCTTTGGCAAGAGATAAAGACCTTGGATTAAAGAATGTGAGGCATTTTATTCTTGATGAATGTGACAAGATGCTCGAATCACTTGATATGAGGAGAGATGTGCAGGAGATCTTCAGGTTGACTCCTCATGACAAGCAAGTAATGATGTTTTCAGCAACGCTAAGCAAGGAAATCCGCCCTGTCTGCAAGAAATTTATGCAAGATCCGATGGAAATTTATGTAGATGATGAAGCCAAGTTGACTCTTCATGGTCTCGTTCAGCATTACATCAAATTGAGTGAATCTGAGAAGAACCGCAAGTTGACCGACCTTCTTGATGCGTTGGATTTCAATCAAGTTGTTATTTTTGTAAAAAGTGTGAGCAGAGCAGCTGAGTTGAACAAGTTACTGGCTGACTGTAATTTTCCCTCTATCTGCATTCATTCTGGAATGCCCCAGGAAGAAAGGTTGAAAAGGTACAAGAATTTCAAGGAGGGCCTTTCAAGAATTCTTGTGGCAACAGATTTAGTAGGGAGAGGAATTGACATTGAGCGTGTTAACATTGTTATCAACTATGACATGCCAGATTCTGCTGATACCTACCTACACAGGGTTGGTAGAGCCGGAAGGTTCGGTACTAAAGGGTTGGCAATAACATTTGTCTCATCTGCATCGGACTCTGATGTTCTTAATCAGGTCCAGTCAAGGTTTGAAGTGGACATAAAGGAGCTCCCAGAGCAAATTGATACTTCTACATACATGCAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006974 GO:0007275 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009555 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:1901360 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

344

Amino Acids

39.0

Weight (kDa)

6.38

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 2 - 153 2.7e-36 DEAD/DEAH box helicase
ResIII PF04851 4 - 150 2.9e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 191 - 300 6.5e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 191, 460, 580
AclWI GGATC 2 cut(s) 71, 482
AcsI RAATTY 5 cut(s) 149, 476, 498, 748, 771
AcuI CTGAAG 2 cut(s) 50, 382
AfaI GTAC 3 cut(s) 166, 743, 901
AfiI CCNNNNNNNGG 2 cut(s) 86, 874
AgsI TTSAA 6 cut(s) 616, 664, 736, 754, 768, 977
AjnI CCWGG 2 cut(s) 271, 720
AluBI AGCT 2 cut(s) 656, 994
AluI AGCT 2 cut(s) 656, 994
Alw21I GWGCWC 1 cut(s) 996
Alw26I GTCTC 2 cut(s) 542, 931
AlwI GGATC 2 cut(s) 71, 482
AlwNI CAGNNNCTG 1 cut(s) 854
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 1 cut(s) 760
ApeKI GCWGC 2 cut(s) 98, 653
ApoI RAATTY 5 cut(s) 149, 476, 498, 748, 771
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AspS9I GGNCC 2 cut(s) 760, 961
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 961
BanII GRGCYC 1 cut(s) 996
BarI GAAGNNNNNNTAC 2 cut(s) 883, 915
Bbv12I GWGCWC 1 cut(s) 996
BbvI GCAGC 2 cut(s) 85, 665
BccI CCATC 2 cut(s) 25, 487
BciT130I CCWGG 2 cut(s) 273, 722
BcoDI GTCTC 2 cut(s) 542, 931
BfmI CTRYAG 1 cut(s) 42
BglII AGATCT 1 cut(s) 393
BisI GCNGC 2 cut(s) 99, 654
BlpI GCTNAGC 1 cut(s) 446
BlsI GCNGC 2 cut(s) 100, 655
Bme1390I CCNGG 2 cut(s) 273, 722
Bme18I GGWCC 1 cut(s) 961
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 2 cut(s) 760, 961
BmrFI CCNGG 2 cut(s) 273, 722
BmsI GCATC 3 cut(s) 345, 592, 944
Bpu1102I GCTNAGC 1 cut(s) 446
BsaAI YACGTR 1 cut(s) 168
BsaBI GATNNNNATC 1 cut(s) 615
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 3 cut(s) 272, 307, 720
BsaWI WCCGGW 1 cut(s) 79
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 874
Bse1I ACTGG 2 cut(s) 678, 964
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 2 cut(s) 273, 722
BseDI CCNNGG 3 cut(s) 272, 307, 720
BseGI GGATG 2 cut(s) 10, 36
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 2 cut(s) 86, 874
BseMII CTCAG 2 cut(s) 561, 648
BseNI ACTGG 2 cut(s) 678, 964
BseRI GAGGAG 2 cut(s) 391, 399
BseXI GCAGC 2 cut(s) 85, 665
BsgI GTGCAG 1 cut(s) 407
BshFI GGCC 1 cut(s) 762
BsiHKAI GWGCWC 1 cut(s) 996
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 2 cut(s) 80, 888
BslI CCNNNNNNNGG 2 cut(s) 86, 874
BsmAI GTCTC 2 cut(s) 542, 931
BsmI GAATGC 3 cut(s) 251, 702, 720
BsnI GGCC 1 cut(s) 762
Bso31I GGTCTC 1 cut(s) 542
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 996
Bsp143I GATC 3 cut(s) 76, 393, 487
Bsp1720I GCTNAGC 1 cut(s) 446
BspACI CCGC 3 cut(s) 191, 460, 580
BspANI GGCC 1 cut(s) 762
BspCNI CTCAG 2 cut(s) 562, 649
BspHI TCATGA 2 cut(s) 145, 412
BspMAI CTGCAG 1 cut(s) 46
BspPI GGATC 2 cut(s) 71, 482
BspTNI GGTCTC 1 cut(s) 542
BsrI ACTGG 2 cut(s) 678, 964
BssECI CCNNGG 3 cut(s) 272, 307, 720
BssMI GATC 3 cut(s) 76, 393, 487
BssT1I CCWWGG 1 cut(s) 307
Bst2UI CCWGG 2 cut(s) 273, 722
Bst4CI ACNGT 1 cut(s) 683
Bst6I CTCTTC 2 cut(s) 69, 534
BstBAI YACGTR 1 cut(s) 168
BstDEI CTNAG 3 cut(s) 446, 570, 657
BstF5I GGATG 2 cut(s) 10, 36
BstKTI GATC 3 cut(s) 79, 396, 490
BstMAI GTCTC 2 cut(s) 542, 931
BstMBI GATC 3 cut(s) 76, 393, 487
BstMWI GCNNNNNNNGC 2 cut(s) 468, 1000
BstNI CCWGG 2 cut(s) 273, 722
BstNSI RCATGY 2 cut(s) 847, 1027
BstSCI CCNGG 2 cut(s) 271, 720
BstSFI CTRYAG 1 cut(s) 42
BstSNI TACGTA 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 85, 665
BstX2I RGATCY 3 cut(s) 76, 393, 487
BstYI RGATCY 3 cut(s) 76, 393, 487
BsuRI GGCC 1 cut(s) 762
BtsCI GGATG 2 cut(s) 10, 36
CaiI CAGNNNCTG 1 cut(s) 854
CciI TCATGA 2 cut(s) 145, 412
Cfr13I GGNCC 2 cut(s) 760, 961
Csp6I GTAC 3 cut(s) 165, 742, 900
CviAII CATG 5 cut(s) 146, 413, 533, 844, 1024
CviJI RGCY 8 cut(s) 131, 287, 518, 656, 677, 762, 887, 994
CviKI_1 RGCY 8 cut(s) 131, 287, 518, 656, 677, 762, 887, 994
CviQI GTAC 3 cut(s) 165, 742, 900
DdeI CTNAG 3 cut(s) 446, 570, 657
DpnI GATC 3 cut(s) 78, 395, 489
DpnII GATC 3 cut(s) 76, 393, 487
Eam1104I CTCTTC 2 cut(s) 69, 534
EarI CTCTTC 2 cut(s) 69, 534
EciI GGCGGA 1 cut(s) 449
Ecl136II GAGCTC 1 cut(s) 994
Eco105I TACGTA 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 307
Eco24I GRGCYC 1 cut(s) 996
Eco31I GGTCTC 1 cut(s) 542
Eco47I GGWCC 1 cut(s) 961
Eco53kI GAGCTC 1 cut(s) 994
Eco57I CTGAAG 2 cut(s) 50, 382
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 994
EcoO109I RGGNCCY 1 cut(s) 760
EcoRI GAATTC 1 cut(s) 771
EcoRII CCWGG 2 cut(s) 271, 720
EcoT14I CCWWGG 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 996
ErhI CCWWGG 1 cut(s) 307
FaeI CATG 5 cut(s) 149, 416, 536, 847, 1027
FatI CATG 5 cut(s) 145, 412, 532, 843, 1023
Fnu4HI GCNGC 2 cut(s) 99, 654
FokI GGATG 2 cut(s) 17, 43
FriOI GRGCYC 1 cut(s) 996
Fsp4HI GCNGC 2 cut(s) 99, 654
GluI GCNGC 2 cut(s) 99, 654
HaeIII GGCC 1 cut(s) 762
HapII CCGG 2 cut(s) 80, 888
Hin1II CATG 5 cut(s) 149, 416, 536, 847, 1027
HincII GTYRAC 5 cut(s) 60, 405, 525, 588, 823
HindII GTYRAC 5 cut(s) 60, 405, 525, 588, 823
HinfI GANTC 7 cut(s) 159, 362, 406, 526, 566, 851, 941
HpaI GTTAAC 1 cut(s) 823
HpaII CCGG 2 cut(s) 80, 888
Hpy166II GTNNAC 7 cut(s) 60, 223, 405, 525, 588, 823, 982
Hpy188I TCNGA 4 cut(s) 492, 571, 940, 946
Hpy188III TCNNGA 6 cut(s) 146, 338, 413, 599, 711, 768
Hpy8I GTNNAC 7 cut(s) 60, 223, 405, 525, 588, 823, 982
HpyAV CCTTC 2 cut(s) 605, 885
HpyCH4III ACNGT 1 cut(s) 683
HpyCH4IV ACGT 1 cut(s) 167
HpyCH4V TGCA 7 cut(s) 44, 388, 471, 484, 702, 935, 1027
HpyF10VI GCNNNNNNNGC 2 cut(s) 468, 1000
HpyF3I CTNAG 3 cut(s) 446, 570, 657
HpySE526I ACGT 1 cut(s) 167
Hsp92II CATG 5 cut(s) 149, 416, 536, 847, 1027
KspAI GTTAAC 1 cut(s) 823
Kzo9I GATC 3 cut(s) 76, 393, 487
LmnI GCTCC 2 cut(s) 991, 999
Lsp1109I GCAGC 2 cut(s) 85, 665
LweI GCATC 3 cut(s) 345, 592, 944
MaeII ACGT 1 cut(s) 167
MaeIII GTNAC 3 cut(s) 208, 347, 669
MalI GATC 3 cut(s) 78, 395, 489
MboI GATC 3 cut(s) 76, 393, 487
MboII GAAGA 5 cut(s) 56, 388, 521, 586, 737
MflI RGATCY 3 cut(s) 76, 393, 487
MhlI GDGCHC 1 cut(s) 996
MlyI GAGTC 3 cut(s) 400, 520, 935
MmeI TCCRAC 2 cut(s) 244, 588
MnlI CCTC 8 cut(s) 197, 261, 318, 369, 420, 703, 751, 794
MseI TTAA 5 cut(s) 182, 216, 314, 822, 954
MspA1I CMGCKG 1 cut(s) 656
MspI CCGG 2 cut(s) 80, 888
MspR9I CCNGG 2 cut(s) 273, 722
Mva1269I GAATGC 3 cut(s) 251, 702, 720
MvaI CCWGG 2 cut(s) 273, 722
MwoI GCNNNNNNNGC 2 cut(s) 468, 1000
NdeII GATC 3 cut(s) 76, 393, 487
NlaIII CATG 5 cut(s) 149, 416, 536, 847, 1027
NmuCI GTSAC 1 cut(s) 347
NspI RCATGY 2 cut(s) 847, 1027
PagI TCATGA 2 cut(s) 145, 412
PctI GAATGC 3 cut(s) 251, 702, 720
PfeI GAWTC 4 cut(s) 159, 362, 566, 851
PkrI GCNGC 2 cut(s) 100, 655
PleI GAGTC 3 cut(s) 400, 520, 935
PpsI GAGTC 3 cut(s) 400, 520, 935
Ppu21I YACGTR 1 cut(s) 168
Psp124BI GAGCTC 1 cut(s) 996
Psp6I CCWGG 2 cut(s) 271, 720
PspGI CCWGG 2 cut(s) 271, 720
PspPI GGNCC 2 cut(s) 760, 961
PstI CTGCAG 1 cut(s) 46
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 3 cut(s) 76, 393, 487
PvuII CAGCTG 1 cut(s) 656
RsaI GTAC 3 cut(s) 166, 743, 901
RsaNI GTAC 3 cut(s) 165, 742, 900
SacI GAGCTC 1 cut(s) 996
SaqAI TTAA 5 cut(s) 182, 216, 314, 822, 954
SatI GCNGC 2 cut(s) 99, 654
Sau3AI GATC 3 cut(s) 76, 393, 487
Sau96I GGNCC 2 cut(s) 760, 961
SchI GAGTC 3 cut(s) 400, 520, 935
ScrFI CCNGG 2 cut(s) 273, 722
SduI GDGCHC 1 cut(s) 996
SfaNI GCATC 3 cut(s) 345, 592, 944
SfcI CTRYAG 1 cut(s) 42
SinI GGWCC 1 cut(s) 961
SnaBI TACGTA 1 cut(s) 168
SsiI CCGC 3 cut(s) 191, 460, 580
SstI GAGCTC 1 cut(s) 996
StyD4I CCNGG 2 cut(s) 271, 720
StyI CCWWGG 1 cut(s) 307
TaaI ACNGT 1 cut(s) 683
TaiI ACGT 1 cut(s) 170
TaqI TCGA 1 cut(s) 360
TaqII GACCGA 1 cut(s) 605
TfiI GAWTC 4 cut(s) 159, 362, 566, 851
Tru1I TTAA 5 cut(s) 182, 216, 314, 822, 954
Tru9I TTAA 5 cut(s) 182, 216, 314, 822, 954
TseFI GTSAC 1 cut(s) 347
TseI GCWGC 2 cut(s) 98, 653
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 6 cut(s) 162, 258, 357, 521, 528, 695
VpaK11BI GGWCC 1 cut(s) 961
XapI RAATTY 5 cut(s) 149, 476, 498, 748, 771
XceI RCATGY 2 cut(s) 847, 1027
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.