Rorug06G0278500

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001445
Physical Location & Seq
Reverse (-)
42609463 .. 42610107
645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0278500.1

Sequence Viewer

Length: 474 bp
ATGAAGGAACATAAGTGGGTACAAGGGACAAAGGTGAAGGTGTTGAGTTATAATGAGTCAGTTGAGATTGTGTTCCAAGGGACTGATGTGCAAGGAGCCTCTGTGAATCATCCACTGCATATGCATGGATATAAGTTTTATGTCGTTGGATATGGTTTCGGAAATTATGACAATGAGACCGACTCGAAAGGGTTTAATCTTGTTGATCCTCCTCATGTCACTACATTTGGAATTCCCAAAAATGGATGGTTGGCGATCAGATTCATAGCGAATAATCCTGGTGTTTGGTTTTGGCATTGTCATATGGAGAGACACCTGACTTTGGGTATGGAAGCTGCATTCATAGTGAAGAATGGGGACACCGCTGAAACTAGCATACTCGAACCCCCAGCTTACATGCCTTCCTGTAATGTTCCATTGGTTTCTCGTATCGAAAATTCTGATGAGTTTGTTGAAAAGTTAATAGATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

157

Amino Acids

17.77

Weight (kDa)

5.69

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 5 - 119 3.9e-37 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 51
AciI CCGC 1 cut(s) 363
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 2 cut(s) 231, 436
AfaI GTAC 1 cut(s) 21
AfiI CCNNNNNNNGG 2 cut(s) 242, 322
AgsI TTSAA 1 cut(s) 455
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 2 cut(s) 335, 392
AluI AGCT 2 cut(s) 335, 392
Alw26I GTCTC 2 cut(s) 170, 304
AlwI GGATC 1 cut(s) 200
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 2 cut(s) 231, 436
AsuHPI GGTGA 1 cut(s) 46
BbvI GCAGC 1 cut(s) 322
BccI CCATC 1 cut(s) 240
BciT130I CCWGG 1 cut(s) 279
BcoDI GTCTC 2 cut(s) 170, 304
BfaI CTAG 1 cut(s) 372
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
Bme1390I CCNGG 1 cut(s) 279
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 1 cut(s) 279
BplI GAGNNNNNCTC 2 cut(s) 167, 199
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 322
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 76
BseGI GGATG 2 cut(s) 109, 251
BseLI CCNNNNNNNGG 2 cut(s) 242, 322
BseRI GAGGAG 1 cut(s) 201
BseXI GCAGC 1 cut(s) 322
BseYI CCCAGC 1 cut(s) 388
BslFI GGGAC 3 cut(s) 40, 94, 371
BslI CCNNNNNNNGG 2 cut(s) 242, 322
BsmAI GTCTC 2 cut(s) 170, 304
BsmFI GGGAC 3 cut(s) 40, 94, 371
BsmI GAATGC 1 cut(s) 338
Bso31I GGTCTC 1 cut(s) 170
Bsp143I GATC 3 cut(s) 205, 255, 466
BspACI CCGC 1 cut(s) 363
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 3 cut(s) 205, 255, 466
BssT1I CCWWGG 1 cut(s) 76
Bst2UI CCWGG 1 cut(s) 279
BstF5I GGATG 2 cut(s) 109, 251
BstKTI GATC 3 cut(s) 208, 258, 469
BstMAI GTCTC 2 cut(s) 170, 304
BstMBI GATC 3 cut(s) 205, 255, 466
BstNI CCWGG 1 cut(s) 279
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 277
BstV1I GCAGC 1 cut(s) 322
BtsCI GGATG 2 cut(s) 109, 251
BtsI GCAGTG 1 cut(s) 113
BtsIMutI CAGTG 1 cut(s) 113
Csp6I GTAC 1 cut(s) 20
CviAII CATG 3 cut(s) 125, 215, 397
CviJI RGCY 3 cut(s) 98, 335, 392
CviKI_1 RGCY 3 cut(s) 98, 335, 392
CviQI GTAC 1 cut(s) 20
DpnI GATC 3 cut(s) 207, 257, 468
DpnII GATC 3 cut(s) 205, 255, 466
Eco130I CCWWGG 1 cut(s) 76
Eco31I GGTCTC 1 cut(s) 170
EcoRI GAATTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 76
EcoT22I ATGCAT 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 76
FaeI CATG 3 cut(s) 128, 218, 400
FaqI GGGAC 3 cut(s) 40, 94, 371
FatI CATG 3 cut(s) 124, 214, 396
FauNDI CATATG 2 cut(s) 120, 303
Fnu4HI GCNGC 1 cut(s) 336
FokI GGATG 2 cut(s) 96, 258
Fsp4HI GCNGC 1 cut(s) 336
FspBI CTAG 1 cut(s) 372
GluI GCNGC 1 cut(s) 336
GsaI CCCAGC 1 cut(s) 392
Hin1II CATG 3 cut(s) 128, 218, 400
HinfI GANTC 4 cut(s) 56, 106, 182, 261
HphI GGTGA 1 cut(s) 46
Hpy188I TCNGA 3 cut(s) 161, 260, 442
HpyAV CCTTC 2 cut(s) 31, 411
HpyCH4V TGCA 4 cut(s) 91, 118, 124, 338
Hsp92II CATG 3 cut(s) 128, 218, 400
Kzo9I GATC 3 cut(s) 205, 255, 466
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 5 cut(s) 264, 291, 329, 402, 418
Lsp1109I GCAGC 1 cut(s) 322
MaeI CTAG 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 217
MalI GATC 3 cut(s) 207, 257, 468
MboI GATC 3 cut(s) 205, 255, 466
MboII GAAGA 1 cut(s) 361
MluCI AATT 3 cut(s) 163, 231, 436
MlyI GAGTC 2 cut(s) 65, 176
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 3 cut(s) 109, 219, 222
Mph1103I ATGCAT 1 cut(s) 126
MseI TTAA 2 cut(s) 195, 461
MslI CAYNNNNRTG 1 cut(s) 123
MspA1I CMGCKG 1 cut(s) 365
MspR9I CCNGG 1 cut(s) 279
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 1 cut(s) 279
NdeI CATATG 2 cut(s) 120, 303
NdeII GATC 3 cut(s) 205, 255, 466
NlaIII CATG 3 cut(s) 128, 218, 400
NlaIV GGNNCC 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 217
NsiI ATGCAT 1 cut(s) 126
NspI RCATGY 1 cut(s) 400
PctI GAATGC 1 cut(s) 338
PfeI GAWTC 2 cut(s) 106, 261
PkrI GCNGC 1 cut(s) 337
PleI GAGTC 2 cut(s) 64, 176
PpsI GAGTC 2 cut(s) 64, 176
PsiI TTATAA 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 277
PspFI CCCAGC 1 cut(s) 388
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 1 cut(s) 97
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
RseI CAYNNNNRTG 1 cut(s) 123
SaqAI TTAA 2 cut(s) 195, 461
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 3 cut(s) 205, 255, 466
SchI GAGTC 2 cut(s) 65, 176
ScrFI CCNGG 1 cut(s) 279
SetI ASST 5 cut(s) 36, 42, 318, 337, 394
SmiMI CAYNNNNRTG 1 cut(s) 123
Sse9I AATT 3 cut(s) 163, 231, 436
SsiI CCGC 1 cut(s) 363
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 1 cut(s) 76
TaqI TCGA 3 cut(s) 185, 381, 432
TaqII GACCGA 1 cut(s) 194
TasI AATT 3 cut(s) 163, 231, 436
TfiI GAWTC 2 cut(s) 106, 261
Tru1I TTAA 2 cut(s) 195, 461
Tru9I TTAA 2 cut(s) 195, 461
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 1 cut(s) 335
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 3 cut(s) 17, 253, 331
TspRI CASTG 1 cut(s) 120
XapI RAATTY 2 cut(s) 231, 436
XceI RCATGY 1 cut(s) 400
XspI CTAG 1 cut(s) 372
Zsp2I ATGCAT 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.