FvH4_2g30810

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002944
Physical Location & Seq
Forward (+)
23781129 .. 23781892
764 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30810.t1

Sequence Viewer

Length: 471 bp
ATGGCATCAGTTTCTGTAATGGATGAGTCTGCTGCTGCTGTTGCCGATGAGGTCGAGCTGGTGACGTGTCACTGCTGCGGGCTGGAAGAGGAGTGCACGCCGGGATACATAGCGCGGGAAAAGGAGAAACACCAGGGACGTTGGATCTGCGGGCTTTGCGCGACGGCGATCGAGGACGATAAGATGAAGTTGCGGAGGGAGATCACTGCGGATGAGGCCATTAGGCGACACGTCAAGCTTGTCGAGAACTTTCGGTCGTCGTCGTCGAGGAGTATGGATTCTCCCGCGGCCGATGAGAAGGAGGAGGAGACGGCGGACTTGTTGATCTCGAAAGTAATGCAGCTCCTGCGGCGTACATTGGACTCGCCGAGAAGGACAGGGTCTGGTTCCGGTGGCGGGGCGTTTGATAGGCGTCTGTTAAGGTCGAAGAGCTGTTTTTCGACAGTAGGAGAGAAGGGTCGTCTACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

157

Amino Acids

17.28

Weight (kDa)

6.33

Isoelectric Point (pI)

79.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 18 - 120 5.5e-25 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 463
AccII CGCG 3 cut(s) 115, 161, 287
AclWI GGATC 1 cut(s) 152
AcoI YGGCCR 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 412
AfaI GTAC 1 cut(s) 355
AfiI CCNNNNNNNGG 1 cut(s) 396
AflIII ACRYGT 2 cut(s) 65, 229
AjiI CACGTC 2 cut(s) 66, 232
AjnI CCWGG 1 cut(s) 132
AluBI AGCT 4 cut(s) 58, 238, 343, 432
AluI AGCT 4 cut(s) 58, 238, 343, 432
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 302
Alw44I GTGCAC 1 cut(s) 94
AlwI GGATC 1 cut(s) 152
AlwNI CAGNNNCTG 3 cut(s) 14, 346, 383
AoxI GGCC 2 cut(s) 216, 288
ApaLI GTGCAC 1 cut(s) 94
ApeKI GCWGC 4 cut(s) 32, 35, 75, 340
AspLEI GCGC 2 cut(s) 115, 161
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 73
BaeGI GKGCMC 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 4 cut(s) 19, 22, 62, 352
BceAI ACGGC 2 cut(s) 180, 327
BcgI CGANNNNNNTGC 2 cut(s) 319, 353
BciT130I CCWGG 1 cut(s) 134
BciVI GTATCC 1 cut(s) 98
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 302
BfuI GTATCC 1 cut(s) 98
BisI GCNGC 6 cut(s) 33, 36, 76, 288, 341, 350
BlsI GCNGC 6 cut(s) 34, 37, 77, 289, 342, 351
Bme1390I CCNGG 2 cut(s) 102, 134
BmgBI CACGTC 2 cut(s) 66, 232
BmiI GGNNCC 1 cut(s) 388
BmrFI CCNGG 2 cut(s) 102, 134
BmsI GCATC 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 412
BsaJI CCNNGG 2 cut(s) 133, 285
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 1 cut(s) 396
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 2 cut(s) 133, 285
BseGI GGATG 2 cut(s) 28, 217
BseLI CCNNNNNNNGG 1 cut(s) 396
BseRI GAGGAG 4 cut(s) 104, 283, 317, 320
BseSI GKGCMC 1 cut(s) 98
BseX3I CGGCCG 1 cut(s) 288
BseXI GCAGC 4 cut(s) 19, 22, 62, 352
Bsh1236I CGCG 3 cut(s) 115, 161, 287
Bsh1285I CGRYCG 3 cut(s) 171, 257, 291
BshFI GGCC 2 cut(s) 218, 290
BsiEI CGRYCG 3 cut(s) 171, 257, 291
BsiHKAI GWGCWC 1 cut(s) 98
BsiSI CCGG 2 cut(s) 101, 390
BslFI GGGAC 1 cut(s) 150
BslI CCNNNNNNNGG 1 cut(s) 396
BsmAI GTCTC 1 cut(s) 302
BsmBI CGTCTC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 150
BsnI GGCC 2 cut(s) 218, 290
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 4 cut(s) 144, 168, 201, 324
BspANI GGCC 2 cut(s) 218, 290
BspFNI CGCG 3 cut(s) 115, 161, 287
BspLI GGNNCC 1 cut(s) 388
BspPI GGATC 1 cut(s) 152
BspQI GCTCTTC 1 cut(s) 422
BssECI CCNNGG 2 cut(s) 133, 285
BssMI GATC 4 cut(s) 144, 168, 201, 324
BssNI GRCGYC 1 cut(s) 412
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 1 cut(s) 445
Bst6I CTCTTC 2 cut(s) 81, 422
BstACI GRCGYC 1 cut(s) 412
BstAPI GCANNNNNTGC 1 cut(s) 346
BstC8I GCNNGC 3 cut(s) 80, 98, 152
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 2 cut(s) 28, 217
BstFNI CGCG 3 cut(s) 115, 161, 287
BstHHI GCGC 2 cut(s) 115, 161
BstKTI GATC 4 cut(s) 147, 171, 204, 327
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 4 cut(s) 144, 168, 201, 324
BstMCI CGRYCG 3 cut(s) 171, 257, 291
BstMWI GCNNNNNNNGC 5 cut(s) 41, 156, 215, 346, 349
BstNI CCWGG 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 100, 132
BstSLI GKGCMC 1 cut(s) 98
BstUI CGCG 3 cut(s) 115, 161, 287
BstV1I GCAGC 4 cut(s) 19, 22, 62, 352
BstX2I RGATCY 1 cut(s) 144
BstYI RGATCY 1 cut(s) 144
BstZI CGGCCG 1 cut(s) 288
BsuI GTATCC 1 cut(s) 98
BsuRI GGCC 2 cut(s) 218, 290
BtgI CCRYGG 1 cut(s) 285
BtrI CACGTC 2 cut(s) 66, 232
BtsCI GGATG 2 cut(s) 28, 217
BtsI GCAGTG 2 cut(s) 70, 204
BtsIMutI CAGTG 2 cut(s) 70, 204
Cac8I GCNNGC 3 cut(s) 80, 98, 152
CaiI CAGNNNCTG 3 cut(s) 14, 346, 383
CfoI GCGC 2 cut(s) 115, 161
Cfr42I CCGCGG 1 cut(s) 288
CseI GACGC 1 cut(s) 401
Csp6I GTAC 1 cut(s) 354
CviJI RGCY 8 cut(s) 58, 82, 154, 218, 238, 290, 343, 432
CviKI_1 RGCY 8 cut(s) 58, 82, 154, 218, 238, 290, 343, 432
CviQI GTAC 1 cut(s) 354
DpnI GATC 4 cut(s) 146, 170, 203, 326
DpnII GATC 4 cut(s) 144, 168, 201, 324
EaeI YGGCCR 1 cut(s) 288
EagI CGGCCG 1 cut(s) 288
Eam1104I CTCTTC 2 cut(s) 81, 422
EarI CTCTTC 2 cut(s) 81, 422
EciI GGCGGA 1 cut(s) 329
EclXI CGGCCG 1 cut(s) 288
Eco52I CGGCCG 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 132
Esp3I CGTCTC 1 cut(s) 302
FaiI YATR 2 cut(s) 110, 275
FaqI GGGAC 1 cut(s) 150
FauI CCCGC 5 cut(s) 71, 108, 143, 292, 389
FblI GTMKAC 1 cut(s) 463
Fnu4HI GCNGC 6 cut(s) 33, 36, 76, 288, 341, 350
FokI GGATG 2 cut(s) 35, 224
Fsp4HI GCNGC 6 cut(s) 33, 36, 76, 288, 341, 350
GlaI GCGC 2 cut(s) 114, 160
GluI GCNGC 6 cut(s) 33, 36, 76, 288, 341, 350
HaeIII GGCC 2 cut(s) 218, 290
HapII CCGG 2 cut(s) 101, 390
HgaI GACGC 1 cut(s) 401
HhaI GCGC 2 cut(s) 115, 161
Hin1I GRCGYC 1 cut(s) 412
Hin6I GCGC 2 cut(s) 113, 159
HinP1I GCGC 2 cut(s) 113, 159
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 3 cut(s) 26, 278, 362
HpaII CCGG 2 cut(s) 101, 390
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 96, 464
Hpy188III TCNNGA 2 cut(s) 244, 328
Hpy8I GTNNAC 2 cut(s) 96, 464
Hpy99I CGWCG 4 cut(s) 166, 262, 265, 268
HpyAV CCTTC 3 cut(s) 292, 366, 448
HpyCH4III ACNGT 1 cut(s) 445
HpyCH4IV ACGT 4 cut(s) 65, 139, 231, 466
HpyCH4V TGCA 2 cut(s) 96, 340
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 156, 215, 346, 349
HpySE526I ACGT 4 cut(s) 65, 139, 231, 466
Hsp92I GRCGYC 1 cut(s) 412
HspAI GCGC 2 cut(s) 113, 159
KspI CCGCGG 1 cut(s) 288
Kzo9I GATC 4 cut(s) 144, 168, 201, 324
LguI GCTCTTC 1 cut(s) 422
LmnI GCTCC 1 cut(s) 348
LpnPI CCDG 9 cut(s) 44, 68, 114, 119, 146, 359, 363, 369, 403
Lsp1109I GCAGC 4 cut(s) 19, 22, 62, 352
LweI GCATC 1 cut(s) 14
MaeII ACGT 4 cut(s) 65, 139, 231, 466
MaeIII GTNAC 2 cut(s) 61, 68
MalI GATC 4 cut(s) 146, 170, 203, 326
MboI GATC 4 cut(s) 144, 168, 201, 324
MboII GAAGA 2 cut(s) 98, 439
MflI RGATCY 1 cut(s) 144
MhlI GDGCHC 1 cut(s) 98
MlyI GAGTC 2 cut(s) 35, 356
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 8 cut(s) 43, 82, 166, 189, 208, 261, 295, 298
MseI TTAA 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 2 cut(s) 101, 390
MspR9I CCNGG 2 cut(s) 102, 134
MvaI CCWGG 1 cut(s) 134
MvnI CGCG 3 cut(s) 115, 161, 287
MwoI GCNNNNNNNGC 5 cut(s) 41, 156, 215, 346, 349
NciI CCSGG 1 cut(s) 102
NdeII GATC 4 cut(s) 144, 168, 201, 324
NlaIV GGNNCC 1 cut(s) 388
NmeAIII GCCGAG 1 cut(s) 393
NmuCI GTSAC 2 cut(s) 61, 68
PciSI GCTCTTC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PfeI GAWTC 1 cut(s) 278
PflFI GACNNNGTC 1 cut(s) 379
PkrI GCNGC 6 cut(s) 34, 37, 77, 289, 342, 351
Ple19I CGATCG 1 cut(s) 171
PleI GAGTC 2 cut(s) 34, 356
PpsI GAGTC 2 cut(s) 34, 356
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 388
PstNI CAGNNNCTG 3 cut(s) 14, 346, 383
PsuI RGATCY 1 cut(s) 144
PsyI GACNNNGTC 1 cut(s) 379
PvuI CGATCG 1 cut(s) 171
RsaI GTAC 1 cut(s) 355
RsaNI GTAC 1 cut(s) 354
SacII CCGCGG 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 422
SaqAI TTAA 1 cut(s) 419
SatI GCNGC 6 cut(s) 33, 36, 76, 288, 341, 350
Sau3AI GATC 4 cut(s) 144, 168, 201, 324
SchI GAGTC 2 cut(s) 35, 356
ScrFI CCNGG 2 cut(s) 102, 134
SduI GDGCHC 1 cut(s) 98
SfaNI GCATC 1 cut(s) 14
Sfr303I CCGCGG 1 cut(s) 288
SgrBI CCGCGG 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 100, 132
TaaI ACNGT 1 cut(s) 445
TaiI ACGT 4 cut(s) 68, 142, 234, 469
TaqI TCGA 7 cut(s) 54, 171, 243, 266, 329, 425, 440
TaqII GACCGA 1 cut(s) 243
TauI GCSGC 2 cut(s) 290, 352
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 1 cut(s) 419
Tru9I TTAA 1 cut(s) 419
TscAI CASTG 2 cut(s) 77, 211
TseFI GTSAC 2 cut(s) 61, 68
TseI GCWGC 4 cut(s) 32, 35, 75, 340
Tsp45I GTSAC 2 cut(s) 61, 68
TspDTI ATGAA 1 cut(s) 200
TspRI CASTG 2 cut(s) 77, 211
Tth111I GACNNNGTC 1 cut(s) 379
VneI GTGCAC 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.