MD08G1057000.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002944
Physical Location & Seq
Forward (+)
4421197 .. 4421664
468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1057000.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGAGTCGGCGGAGGCGAAGAGGATTTTGGAGCCGGAGGAGGAGGTGGTGATGAAGTGCTACTGCTGCGGTTTGAAAGAGGAGTGCACACCGGTGTACGTAGCGACGGTGAAGGAGAGGCACCAGGGCCAGTGGATTTGCGGACTCTGCACTGAGGTGATCAAGGACCAGAACTCGAGGTCGTCGAGGAAGATTAGCACGGAGGAGGCCCTGAAGCGGCACGTCCGCTTCTGCGAGGAGTTTCCGACGTCGAGTCCTCCCGCGAACCCGACGGAGGATCTGACATCGGCGATGAAACAGCTGTTGTGGCGGACTCTGGACTCGCCGAGGAGGGAGAGGCTGGGCGACTGCAGGCCGACGATGGTGAGGTCCAGGCCGGTCCTGAGATTTTGGAGCTCCTTGCATGGTCTAAACTTTTGGTTGCTATATTTTTTTTTATATTTTATATTTTTATTGTGTGTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

156

Amino Acids

18.19

Weight (kDa)

8.08

Isoelectric Point (pI)

75.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 18 - 108 9e-35 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 120
AccII CGCG 1 cut(s) 263
AciI CCGC 7 cut(s) 11, 69, 141, 217, 226, 261, 310
AclWI GGATC 1 cut(s) 285
AcuI CTGAAG 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 248
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 216, 274
AgeI ACCGGT 1 cut(s) 91
AgsI TTSAA 1 cut(s) 76
AhdI GACNNNNNGTC 1 cut(s) 252
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 2 cut(s) 123, 371
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AleI CACNNNNGTG 2 cut(s) 92, 155
AluBI AGCT 2 cut(s) 301, 396
AluI AGCT 2 cut(s) 301, 396
Alw21I GWGCWC 2 cut(s) 89, 398
Alw44I GTGCAC 1 cut(s) 85
AlwI GGATC 1 cut(s) 285
Ama87I CYCGRG 1 cut(s) 175
AoxI GGCC 4 cut(s) 127, 207, 353, 374
ApaLI GTGCAC 1 cut(s) 85
ApeKI GCWGC 1 cut(s) 66
AsiGI ACCGGT 1 cut(s) 91
AspS9I GGNCC 5 cut(s) 127, 166, 208, 369, 379
AsuHPI GGTGA 4 cut(s) 61, 121, 169, 376
AvaI CYCGRG 1 cut(s) 175
AvaII GGWCC 3 cut(s) 166, 369, 379
BaeGI GKGCMC 1 cut(s) 89
BanI GGYRCC 1 cut(s) 120
BanII GRGCYC 1 cut(s) 398
Bbv12I GWGCWC 2 cut(s) 89, 398
BbvI GCAGC 1 cut(s) 53
BccI CCATC 1 cut(s) 355
BciT130I CCWGG 2 cut(s) 125, 373
BclI TGATCA 1 cut(s) 159
BfmI CTRYAG 1 cut(s) 349
BisI GCNGC 2 cut(s) 67, 218
BlsI GCNGC 2 cut(s) 68, 219
Bme1390I CCNGG 2 cut(s) 125, 373
Bme18I GGWCC 3 cut(s) 166, 369, 379
BmeRI GACNNNNNGTC 1 cut(s) 252
BmeT110I CYCGRG 1 cut(s) 175
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 5 cut(s) 127, 166, 208, 369, 379
BmiI GGNNCC 2 cut(s) 33, 122
BmrFI CCNGG 2 cut(s) 125, 373
BsaAI YACGTR 1 cut(s) 100
BsaHI GRCGYC 1 cut(s) 248
BsaJI CCNNGG 2 cut(s) 124, 326
BsaWI WCCGGW 1 cut(s) 91
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 274
Bse118I RCCGGY 2 cut(s) 91, 376
Bse1I ACTGG 1 cut(s) 130
BseBI CCWGG 2 cut(s) 125, 373
BseDI CCNNGG 2 cut(s) 124, 326
BseLI CCNNNNNNNGG 2 cut(s) 216, 274
BseMII CTCAG 2 cut(s) 144, 374
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 6 cut(s) 53, 56, 95, 218, 251, 343
BseSI GKGCMC 1 cut(s) 89
BseXI GCAGC 1 cut(s) 53
BseYI CCCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 263
BshFI GGCC 4 cut(s) 129, 209, 355, 376
BshNI GGYRCC 1 cut(s) 120
BshTI ACCGGT 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 89, 398
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 3 cut(s) 35, 92, 377
BslI CCNNNNNNNGG 2 cut(s) 216, 274
BsnI GGCC 4 cut(s) 129, 209, 355, 376
BsoBI CYCGRG 1 cut(s) 175
Bsp1286I GDGCHC 2 cut(s) 89, 398
Bsp143I GATC 2 cut(s) 159, 277
BspACI CCGC 7 cut(s) 11, 69, 141, 217, 226, 261, 310
BspANI GGCC 4 cut(s) 129, 209, 355, 376
BspCNI CTCAG 2 cut(s) 145, 375
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 2 cut(s) 33, 122
BspMAI CTGCAG 1 cut(s) 353
BspPI GGATC 1 cut(s) 285
BspT107I GGYRCC 1 cut(s) 120
BsrFI RCCGGY 2 cut(s) 91, 376
BsrI ACTGG 1 cut(s) 130
BssAI RCCGGY 2 cut(s) 91, 376
BssECI CCNNGG 2 cut(s) 124, 326
BssMI GATC 2 cut(s) 159, 277
BssNI GRCGYC 1 cut(s) 248
Bst2UI CCWGG 2 cut(s) 125, 373
Bst4CI ACNGT 1 cut(s) 109
Bst6I CTCTTC 1 cut(s) 14
BstACI GRCGYC 1 cut(s) 248
BstBAI YACGTR 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 2 cut(s) 153, 383
BstFNI CGCG 1 cut(s) 263
BstKTI GATC 2 cut(s) 162, 280
BstMBI GATC 2 cut(s) 159, 277
BstMWI GCNNNNNNNGC 3 cut(s) 66, 147, 307
BstNI CCWGG 2 cut(s) 125, 373
BstSCI CCNGG 2 cut(s) 123, 371
BstSFI CTRYAG 1 cut(s) 349
BstSLI GKGCMC 1 cut(s) 89
BstSNI TACGTA 1 cut(s) 100
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 4 cut(s) 129, 209, 355, 376
BtgZI GCGATG 1 cut(s) 305
BtrI CACGTC 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 137, 150
Cac8I GCNNGC 1 cut(s) 353
Cfr10I RCCGGY 2 cut(s) 91, 376
Cfr13I GGNCC 5 cut(s) 127, 166, 208, 369, 379
Csp6I GTAC 1 cut(s) 97
CspAI ACCGGT 1 cut(s) 91
CviAII CATG 1 cut(s) 404
CviJI RGCY 8 cut(s) 34, 129, 209, 301, 340, 355, 376, 396
CviKI_1 RGCY 8 cut(s) 34, 129, 209, 301, 340, 355, 376, 396
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 2 cut(s) 153, 383
DpnI GATC 2 cut(s) 161, 279
DpnII GATC 2 cut(s) 159, 277
DriI GACNNNNNGTC 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 14
Eam1105I GACNNNNNGTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 14
EciI GGCGGA 2 cut(s) 26, 325
Ecl136II GAGCTC 1 cut(s) 396
Eco105I TACGTA 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 398
Eco47I GGWCC 3 cut(s) 166, 369, 379
Eco53kI GAGCTC 1 cut(s) 396
Eco57I CTGAAG 1 cut(s) 233
Eco88I CYCGRG 1 cut(s) 175
EcoICRI GAGCTC 1 cut(s) 396
EcoO109I RGGNCCY 1 cut(s) 208
EcoRII CCWGG 2 cut(s) 123, 371
EcoT38I GRGCYC 1 cut(s) 398
FaeI CATG 1 cut(s) 407
FaiI YATR 4 cut(s) 405, 427, 439, 446
FatI CATG 1 cut(s) 403
FauI CCCGC 1 cut(s) 268
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 2 cut(s) 67, 218
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 2 cut(s) 67, 218
GluI GCNGC 2 cut(s) 67, 218
GsaI CCCAGC 1 cut(s) 344
HaeIII GGCC 4 cut(s) 129, 209, 355, 376
HapII CCGG 3 cut(s) 35, 92, 377
Hin1I GRCGYC 1 cut(s) 248
Hin1II CATG 1 cut(s) 407
HinfI GANTC 5 cut(s) 5, 144, 253, 313, 320
HpaII CCGG 3 cut(s) 35, 92, 377
HphI GGTGA 4 cut(s) 61, 121, 169, 376
Hpy166II GTNNAC 2 cut(s) 87, 97
Hpy188I TCNGA 2 cut(s) 246, 282
Hpy188III TCNNGA 2 cut(s) 317, 382
Hpy8I GTNNAC 2 cut(s) 87, 97
Hpy99I CGWCG 6 cut(s) 109, 187, 250, 253, 274, 361
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 3 cut(s) 99, 222, 248
HpyCH4V TGCA 4 cut(s) 87, 150, 351, 403
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 147, 307
HpyF3I CTNAG 2 cut(s) 153, 383
HpySE526I ACGT 3 cut(s) 99, 222, 248
Hsp92I GRCGYC 1 cut(s) 248
Hsp92II CATG 1 cut(s) 407
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 2 cut(s) 159, 277
LmnI GCTCC 3 cut(s) 31, 393, 401
Lsp1109I GCAGC 1 cut(s) 53
MaeII ACGT 3 cut(s) 99, 222, 248
MalI GATC 2 cut(s) 161, 279
MboI GATC 2 cut(s) 159, 277
MboII GAAGA 2 cut(s) 31, 202
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 89, 398
MluCI AATT 1 cut(s) 463
MlyI GAGTC 5 cut(s) 14, 138, 262, 307, 314
MmeI TCCRAC 1 cut(s) 269
MslI CAYNNNNRTG 2 cut(s) 92, 155
MspA1I CMGCKG 1 cut(s) 301
MspI CCGG 3 cut(s) 35, 92, 377
MspR9I CCNGG 2 cut(s) 125, 373
MvaI CCWGG 2 cut(s) 125, 373
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 3 cut(s) 66, 147, 307
NdeII GATC 2 cut(s) 159, 277
NlaIII CATG 1 cut(s) 407
NlaIV GGNNCC 2 cut(s) 33, 122
NmeAIII GCCGAG 1 cut(s) 351
OliI CACNNNNGTG 2 cut(s) 92, 155
PaeR7I CTCGAG 1 cut(s) 175
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 182
PinAI ACCGGT 1 cut(s) 91
PkrI GCNGC 2 cut(s) 68, 219
PleI GAGTC 5 cut(s) 13, 138, 261, 307, 314
PpsI GAGTC 5 cut(s) 13, 138, 261, 307, 314
Ppu21I YACGTR 1 cut(s) 100
Psp124BI GAGCTC 1 cut(s) 398
Psp6I CCWGG 2 cut(s) 123, 371
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 2 cut(s) 123, 371
PspN4I GGNNCC 2 cut(s) 33, 122
PspPI GGNCC 5 cut(s) 127, 166, 208, 369, 379
PspXI VCTCGAGB 1 cut(s) 175
PstI CTGCAG 1 cut(s) 353
PsuI RGATCY 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 301
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
RseI CAYNNNNRTG 2 cut(s) 92, 155
SacI GAGCTC 1 cut(s) 398
SatI GCNGC 2 cut(s) 67, 218
Sau3AI GATC 2 cut(s) 159, 277
Sau96I GGNCC 5 cut(s) 127, 166, 208, 369, 379
SchI GAGTC 5 cut(s) 14, 138, 262, 307, 314
ScrFI CCNGG 2 cut(s) 125, 373
SduI GDGCHC 2 cut(s) 89, 398
SetI ASST 9 cut(s) 48, 102, 159, 182, 225, 251, 303, 371, 398
SfcI CTRYAG 1 cut(s) 349
Sfr274I CTCGAG 1 cut(s) 175
SgrAI CRCCGGYG 1 cut(s) 91
SinI GGWCC 3 cut(s) 166, 369, 379
SlaI CTCGAG 1 cut(s) 175
SmiMI CAYNNNNRTG 2 cut(s) 92, 155
SmlI CTYRAG 1 cut(s) 175
SmoI CTYRAG 1 cut(s) 175
SnaBI TACGTA 1 cut(s) 100
Sse9I AATT 1 cut(s) 463
SsiI CCGC 7 cut(s) 11, 69, 141, 217, 226, 261, 310
SstI GAGCTC 1 cut(s) 398
StyD4I CCNGG 2 cut(s) 123, 371
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 3 cut(s) 102, 225, 251
TaqI TCGA 3 cut(s) 176, 185, 251
TasI AATT 1 cut(s) 463
TauI GCSGC 1 cut(s) 220
TscAI CASTG 2 cut(s) 137, 157
TseI GCWGC 1 cut(s) 66
TspDTI ATGAA 2 cut(s) 68, 308
TspGWI ACGGA 2 cut(s) 215, 287
TspRI CASTG 2 cut(s) 137, 157
VneI GTGCAC 1 cut(s) 85
VpaK11BI GGWCC 3 cut(s) 166, 369, 379
XhoI CTCGAG 1 cut(s) 175
ZraI GACGTC 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.