MD17G1165800.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002944
Physical Location & Seq
Forward (+)
16474891 .. 16476831
1941 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1165800.v1.1.491

Sequence Viewer

Length: 510 bp
ATGTGGTTACTAATAATGGCAATGTCTGCAACTATCATCAGCAGCGACCCGATTGTTCTACCGGCGGCCGCCACCGAAACTCAGCCAACACAAGCCACAACAAAACTCGTTTCCCCAATCGAAATCGAGTTCGTAAAATGTGACTGCTGTGGATTAACAGAAGAGTGCACGCCGGCGTACATTGAGCACGTGAGGGAGAGATATCAGGGCCATTGGATTTGCGGGTTGTGTGCAGAAGCTGTAAAAGATGAGATTGTTAGGTCACAGAAGAGGCTGATGAGCACGGAAGAAGCCATGACCACACACATGAACTTCTGCAAAAAGTTCAAGGCCTCAGGGCCTCCTCCAAACCCTACCATTCACTTGATATCCGCCATGAGGCAGATTCTCCGGCGCAGTTTGGATTCTCCGAGGGGGGGTTTGAGATCAACTCCAAGTAGTCCTACAAAAATTAATGCAGGGAAAGGGCTCAAGAGGTCTGAGAGTTGCTTTCCAACTCTGACTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

170

Amino Acids

18.59

Weight (kDa)

8.66

Isoelectric Point (pI)

79.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 42 - 136 4.8e-42 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 65, 69, 222, 372
AcoI YGGCCR 1 cut(s) 66
AcvI CACGTG 1 cut(s) 190
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 2 cut(s) 378, 416
AgsI TTSAA 1 cut(s) 328
AluBI AGCT 1 cut(s) 239
AluI AGCT 1 cut(s) 239
Alw21I GWGCWC 3 cut(s) 170, 189, 284
Alw44I GTGCAC 1 cut(s) 166
AlwNI CAGNNNCTG 1 cut(s) 239
AoxI GGCC 4 cut(s) 66, 208, 330, 338
ApaLI GTGCAC 1 cut(s) 166
ApeKI GCWGC 1 cut(s) 42
AseI ATTAAT 1 cut(s) 453
AspLEI GCGC 1 cut(s) 396
AspS9I GGNCC 2 cut(s) 208, 338
AxyI CCTNAGG 1 cut(s) 334
BaeGI GKGCMC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 471
BbrPI CACGTG 1 cut(s) 190
Bbv12I GWGCWC 3 cut(s) 170, 189, 284
BbvI GCAGC 1 cut(s) 54
BisI GCNGC 3 cut(s) 43, 66, 69
BlsI GCNGC 3 cut(s) 44, 67, 70
BmgT120I GGNCC 2 cut(s) 208, 338
BplI GAGNNNNNCTC 2 cut(s) 415, 447
BpuEI CTTGAG 1 cut(s) 455
BsaAI YACGTR 1 cut(s) 190
BsaJI CCNNGG 1 cut(s) 410
Bsc4I CCNNNNNNNGG 2 cut(s) 378, 416
Bse118I RCCGGY 2 cut(s) 61, 172
Bse1I ACTGG 1 cut(s) 508
Bse21I CCTNAGG 1 cut(s) 334
Bse3DI GCAATG 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 410
BseLI CCNNNNNNNGG 2 cut(s) 378, 416
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 3 cut(s) 95, 348, 471
BseNI ACTGG 1 cut(s) 508
BseRI GAGGAG 1 cut(s) 333
BseSI GKGCMC 1 cut(s) 170
BseX3I CGGCCG 1 cut(s) 66
BseXI GCAGC 1 cut(s) 54
BsgI GTGCAG 1 cut(s) 252
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 4 cut(s) 68, 210, 332, 340
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 3 cut(s) 170, 189, 284
BsiSI CCGG 3 cut(s) 62, 173, 391
BslI CCNNNNNNNGG 2 cut(s) 378, 416
BsnI GGCC 4 cut(s) 68, 210, 332, 340
Bsp1286I GDGCHC 4 cut(s) 170, 189, 284, 471
Bsp143I GATC 1 cut(s) 425
BspACI CCGC 4 cut(s) 65, 69, 222, 372
BspANI GGCC 4 cut(s) 68, 210, 332, 340
BspCNI CTCAG 3 cut(s) 94, 347, 472
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 2 cut(s) 61, 172
BsrI ACTGG 1 cut(s) 508
BssAI RCCGGY 2 cut(s) 61, 172
BssECI CCNNGG 1 cut(s) 410
BssMI GATC 1 cut(s) 425
Bst6I CTCTTC 2 cut(s) 156, 263
BstAPI GCANNNNNTGC 1 cut(s) 26
BstBAI YACGTR 1 cut(s) 190
BstC8I GCNNGC 2 cut(s) 170, 174
BstDEI CTNAG 3 cut(s) 81, 334, 480
BstHHI GCGC 1 cut(s) 396
BstKTI GATC 1 cut(s) 428
BstMBI GATC 1 cut(s) 425
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstSLI GKGCMC 1 cut(s) 170
BstV1I GCAGC 1 cut(s) 54
BstZI CGGCCG 1 cut(s) 66
Bsu36I CCTNAGG 1 cut(s) 334
BsuRI GGCC 4 cut(s) 68, 210, 332, 340
Cac8I GCNNGC 2 cut(s) 170, 174
CaiI CAGNNNCTG 1 cut(s) 239
CciNI GCGGCCGC 1 cut(s) 66
CfoI GCGC 1 cut(s) 396
Cfr10I RCCGGY 2 cut(s) 61, 172
Cfr13I GGNCC 2 cut(s) 208, 338
Csp6I GTAC 1 cut(s) 178
CviAII CATG 3 cut(s) 295, 307, 376
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 3 cut(s) 81, 334, 480
DpnI GATC 1 cut(s) 427
DpnII GATC 1 cut(s) 425
EaeI YGGCCR 1 cut(s) 66
EagI CGGCCG 1 cut(s) 66
Eam1104I CTCTTC 2 cut(s) 156, 263
EarI CTCTTC 2 cut(s) 156, 263
EciI GGCGGA 1 cut(s) 361
EclXI CGGCCG 1 cut(s) 66
Eco147I AGGCCT 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 471
Eco32I GATATC 2 cut(s) 203, 369
Eco52I CGGCCG 1 cut(s) 66
Eco72I CACGTG 1 cut(s) 190
Eco81I CCTNAGG 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 338
EcoRV GATATC 2 cut(s) 203, 369
EcoT38I GRGCYC 1 cut(s) 471
FaeI CATG 3 cut(s) 298, 310, 379
FaiI YATR 3 cut(s) 296, 308, 377
FatI CATG 3 cut(s) 294, 306, 375
FauI CCCGC 1 cut(s) 215
Fnu4HI GCNGC 3 cut(s) 43, 66, 69
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 3 cut(s) 43, 66, 69
GlaI GCGC 1 cut(s) 395
GluI GCNGC 3 cut(s) 43, 66, 69
HaeIII GGCC 4 cut(s) 68, 210, 332, 340
HapII CCGG 3 cut(s) 62, 173, 391
HhaI GCGC 1 cut(s) 396
Hin1II CATG 3 cut(s) 298, 310, 379
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HinfI GANTC 2 cut(s) 385, 404
HpaII CCGG 3 cut(s) 62, 173, 391
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 3 cut(s) 411, 481, 501
Hpy188III TCNNGA 1 cut(s) 472
Hpy8I GTNNAC 1 cut(s) 168
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 5 cut(s) 29, 168, 233, 318, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 3 cut(s) 81, 334, 480
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 3 cut(s) 298, 310, 379
HspAI GCGC 1 cut(s) 394
KroI GCCGGC 1 cut(s) 172
KroNI GCCGGC 1 cut(s) 174
Kzo9I GATC 1 cut(s) 425
LpnPI CCDG 7 cut(s) 75, 186, 191, 321, 404, 444, 489
Lsp1109I GCAGC 1 cut(s) 54
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 6, 140, 261
MalI GATC 1 cut(s) 427
MboI GATC 1 cut(s) 425
MboII GAAGA 3 cut(s) 173, 280, 299
MhlI GDGCHC 4 cut(s) 170, 189, 284, 471
MluCI AATT 1 cut(s) 450
MnlI CCTC 8 cut(s) 186, 264, 343, 351, 354, 372, 405, 468
MreI CGCCGGCG 1 cut(s) 172
MroNI GCCGGC 1 cut(s) 172
MseI TTAA 2 cut(s) 155, 453
MslI CAYNNNNRTG 1 cut(s) 305
MspI CCGG 3 cut(s) 62, 173, 391
MwoI GCNNNNNNNGC 1 cut(s) 26
NaeI GCCGGC 1 cut(s) 174
NdeII GATC 1 cut(s) 425
NgoMIV GCCGGC 1 cut(s) 172
NlaIII CATG 3 cut(s) 298, 310, 379
NmuCI GTSAC 2 cut(s) 140, 261
NotI GCGGCCGC 1 cut(s) 66
PceI AGGCCT 1 cut(s) 332
PdiI GCCGGC 1 cut(s) 174
PfeI GAWTC 2 cut(s) 385, 404
PkrI GCNGC 3 cut(s) 44, 67, 70
PmaCI CACGTG 1 cut(s) 190
PmlI CACGTG 1 cut(s) 190
Ppu21I YACGTR 1 cut(s) 190
PshBI ATTAAT 1 cut(s) 453
PspCI CACGTG 1 cut(s) 190
PspPI GGNCC 2 cut(s) 208, 338
PstNI CAGNNNCTG 1 cut(s) 239
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
RseI CAYNNNNRTG 1 cut(s) 305
SaqAI TTAA 2 cut(s) 155, 453
SatI GCNGC 3 cut(s) 43, 66, 69
Sau3AI GATC 1 cut(s) 425
Sau96I GGNCC 2 cut(s) 208, 338
SduI GDGCHC 4 cut(s) 170, 189, 284, 471
SetI ASST 4 cut(s) 192, 241, 263, 479
SgrAI CRCCGGYG 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
Sse9I AATT 1 cut(s) 450
SseBI AGGCCT 1 cut(s) 332
SsiI CCGC 4 cut(s) 65, 69, 222, 372
StuI AGGCCT 1 cut(s) 332
TaiI ACGT 1 cut(s) 192
TaqI TCGA 2 cut(s) 120, 126
TasI AATT 1 cut(s) 450
TauI GCSGC 2 cut(s) 68, 71
TfiI GAWTC 2 cut(s) 385, 404
Tru1I TTAA 2 cut(s) 155, 453
Tru9I TTAA 2 cut(s) 155, 453
TseFI GTSAC 2 cut(s) 140, 261
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 2 cut(s) 140, 261
TspDTI ATGAA 1 cut(s) 323
TspGWI ACGGA 1 cut(s) 299
VneI GTGCAC 1 cut(s) 166
VspI ATTAAT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.