Rroxscaffold_152G00434880

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000152
OG0002944
Physical Location & Seq
Reverse (-)
418083 .. 419664
1582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_152G00434880.1

Sequence Viewer

Length: 477 bp
ATGTTTACAACTATAATCAGTGACCCAATGGGAGGTCTATCGGCCCCAGAAAGCCAAGCAGCAGCCCCAAAACTCAATCCCCAATCAGAGATTGAGTCTGCAAAGTGTGACTGCTGTGGGCTCACAGAGGAGTGCACCCCTGCCTATATAGAACATGTCCGAGAGAGAAACATGGGCCATTGGATTTGTGGGTTGTGTTCTGAGGCTATAAAAGATGAGATTGTGAGGTCTGAGAGGCTAGTTAGCACTGAAGAAGCCATGGCTACACATATGAACTTTTGCAAGAAGTTTAAGGCAGCCGGGCCTCCTTTGAACCCTACTATTCATTTGATATCTGCCATGAGACAGATTCTTCGGCGTAGTTTGGACTCTCCGAGGGGCTCGAATTCAACTCCGAATAGTCCGATGAGGATGGGTGGCCGCGCGGCGCTTACTAGGTCCGGGAGTTGCTTTCCTGCCCTGACCGGAGTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

158

Amino Acids

17.11

Weight (kDa)

6.89

Isoelectric Point (pI)

70.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 31 - 124 7.6e-41 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 423, 425
AciI CCGC 2 cut(s) 421, 425
AcoI YGGCCR 1 cut(s) 418
AcsI RAATTY 1 cut(s) 385
AcuI CTGAAG 1 cut(s) 270
AfiI CCNNNNNNNGG 1 cut(s) 32
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 2 cut(s) 313, 390
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 337
Alw44I GTGCAC 1 cut(s) 133
AoxI GGCC 4 cut(s) 42, 175, 302, 418
ApaLI GTGCAC 1 cut(s) 133
ApeKI GCWGC 3 cut(s) 59, 62, 296
ApoI RAATTY 1 cut(s) 385
ArsI GACNNNNNNTTYG 2 cut(s) 336, 368
AspLEI GCGC 2 cut(s) 425, 430
AspS9I GGNCC 4 cut(s) 43, 175, 302, 438
AsuC2I CCSGG 2 cut(s) 301, 442
AvaII GGWCC 1 cut(s) 438
BaeGI GKGCMC 1 cut(s) 137
BanII GRGCYC 2 cut(s) 123, 383
Bbv12I GWGCWC 1 cut(s) 137
BbvI GCAGC 3 cut(s) 71, 74, 308
BccI CCATC 1 cut(s) 406
BcnI CCSGG 2 cut(s) 301, 442
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 2 cut(s) 239, 435
BfoI RGCGCY 1 cut(s) 431
BisI GCNGC 5 cut(s) 60, 63, 297, 421, 426
BlsI GCNGC 5 cut(s) 61, 64, 298, 422, 427
Bme1390I CCNGG 2 cut(s) 301, 442
Bme18I GGWCC 1 cut(s) 438
BmgT120I GGNCC 4 cut(s) 43, 175, 302, 438
BmiI GGNNCC 1 cut(s) 45
BmrFI CCNGG 2 cut(s) 301, 442
BpuMI CCSGG 2 cut(s) 301, 442
BsaJI CCNNGG 2 cut(s) 258, 374
BsaWI WCCGGW 1 cut(s) 464
Bsc4I CCNNNNNNNGG 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 258, 374
BseGI GGATG 1 cut(s) 417
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 2 cut(s) 192, 222
BseRI GAGGAG 1 cut(s) 143
BseSI GKGCMC 1 cut(s) 137
BseXI GCAGC 3 cut(s) 71, 74, 308
Bsh1236I CGCG 2 cut(s) 423, 425
BshFI GGCC 4 cut(s) 44, 177, 304, 420
BsiHKAI GWGCWC 1 cut(s) 137
BsiSI CCGG 3 cut(s) 300, 441, 465
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 337
BsnI GGCC 4 cut(s) 44, 177, 304, 420
Bsp1286I GDGCHC 3 cut(s) 123, 137, 383
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 2 cut(s) 421, 425
BspANI GGCC 4 cut(s) 44, 177, 304, 420
BspCNI CTCAG 2 cut(s) 193, 223
BspFNI CGCG 2 cut(s) 423, 425
BspLI GGNNCC 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 258, 374
BssT1I CCWWGG 1 cut(s) 258
BstDEI CTNAG 2 cut(s) 201, 231
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 417
BstFNI CGCG 2 cut(s) 423, 425
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 2 cut(s) 425, 430
BstMAI GTCTC 1 cut(s) 337
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 299, 440
BstSLI GKGCMC 1 cut(s) 137
BstUI CGCG 2 cut(s) 423, 425
BstV1I GCAGC 3 cut(s) 71, 74, 308
BsuRI GGCC 4 cut(s) 44, 177, 304, 420
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 1 cut(s) 417
BtsIMutI CAGTG 2 cut(s) 25, 246
CfoI GCGC 2 cut(s) 425, 430
Cfr13I GGNCC 4 cut(s) 43, 175, 302, 438
CviAII CATG 4 cut(s) 155, 172, 259, 340
DdeI CTNAG 2 cut(s) 201, 231
EaeI YGGCCR 1 cut(s) 418
Eco130I CCWWGG 1 cut(s) 258
Eco24I GRGCYC 2 cut(s) 123, 383
Eco32I GATATC 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 438
Eco57I CTGAAG 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 385
EcoRV GATATC 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 2 cut(s) 123, 383
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 4 cut(s) 158, 175, 262, 343
FatI CATG 4 cut(s) 154, 171, 258, 339
FauNDI CATATG 1 cut(s) 270
Fnu4HI GCNGC 5 cut(s) 60, 63, 297, 421, 426
FokI GGATG 1 cut(s) 424
FriOI GRGCYC 2 cut(s) 123, 383
Fsp4HI GCNGC 5 cut(s) 60, 63, 297, 421, 426
FspBI CTAG 2 cut(s) 239, 435
GlaI GCGC 2 cut(s) 424, 429
GluI GCNGC 5 cut(s) 60, 63, 297, 421, 426
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 4 cut(s) 44, 177, 304, 420
HapII CCGG 3 cut(s) 300, 441, 465
HhaI GCGC 2 cut(s) 425, 430
Hin1II CATG 4 cut(s) 158, 175, 262, 343
Hin6I GCGC 2 cut(s) 423, 428
HinP1I GCGC 2 cut(s) 423, 428
HinfI GANTC 3 cut(s) 95, 349, 368
HpaII CCGG 3 cut(s) 300, 441, 465
Hpy166II GTNNAC 2 cut(s) 6, 135
Hpy188I TCNGA 7 cut(s) 88, 161, 202, 232, 375, 396, 405
Hpy8I GTNNAC 2 cut(s) 6, 135
HpyCH4V TGCA 3 cut(s) 101, 135, 282
HpyF3I CTNAG 2 cut(s) 201, 231
Hsp92II CATG 4 cut(s) 158, 175, 262, 343
HspAI GCGC 2 cut(s) 423, 428
LpnPI CCDG 6 cut(s) 60, 153, 313, 454, 468, 473
Lsp1109I GCAGC 3 cut(s) 71, 74, 308
MaeI CTAG 2 cut(s) 239, 435
MaeIII GTNAC 2 cut(s) 20, 107
MboII GAAGA 2 cut(s) 263, 344
MhlI GDGCHC 3 cut(s) 123, 137, 383
MluCI AATT 1 cut(s) 385
MlyI GAGTC 2 cut(s) 104, 362
MnlI CCTC 8 cut(s) 26, 121, 196, 219, 228, 315, 369, 402
MseI TTAA 1 cut(s) 291
MspI CCGG 3 cut(s) 300, 441, 465
MspR9I CCNGG 2 cut(s) 301, 442
MteI GCGCNGCGC 1 cut(s) 426
MvnI CGCG 2 cut(s) 423, 425
NciI CCSGG 2 cut(s) 301, 442
NcoI CCATGG 1 cut(s) 258
NdeI CATATG 1 cut(s) 270
NlaIII CATG 4 cut(s) 158, 175, 262, 343
NlaIV GGNNCC 1 cut(s) 45
NmuCI GTSAC 2 cut(s) 20, 107
NspI RCATGY 1 cut(s) 158
PciI ACATGT 1 cut(s) 154
PfeI GAWTC 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 440
PkrI GCNGC 5 cut(s) 61, 64, 298, 422, 427
PleI GAGTC 2 cut(s) 103, 362
PpsI GAGTC 2 cut(s) 103, 362
PscI ACATGT 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 45
PspPI GGNCC 4 cut(s) 43, 175, 302, 438
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 5 cut(s) 60, 63, 297, 421, 426
Sau96I GGNCC 4 cut(s) 43, 175, 302, 438
SchI GAGTC 2 cut(s) 104, 362
ScrFI CCNGG 2 cut(s) 301, 442
SduI GDGCHC 3 cut(s) 123, 137, 383
SetI ASST 3 cut(s) 37, 230, 440
SinI GGWCC 1 cut(s) 438
Sse9I AATT 1 cut(s) 385
SsiI CCGC 2 cut(s) 421, 425
SspMI CTAG 2 cut(s) 239, 435
StyD4I CCNGG 2 cut(s) 299, 440
StyI CCWWGG 1 cut(s) 258
TaqI TCGA 1 cut(s) 383
TasI AATT 1 cut(s) 385
TauI GCSGC 2 cut(s) 423, 428
TfiI GAWTC 1 cut(s) 349
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 2 cut(s) 25, 253
TseFI GTSAC 2 cut(s) 20, 107
TseI GCWGC 3 cut(s) 59, 62, 296
Tsp45I GTSAC 2 cut(s) 20, 107
TspDTI ATGAA 2 cut(s) 287, 314
TspRI CASTG 2 cut(s) 25, 253
VneI GTGCAC 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 438
XapI RAATTY 1 cut(s) 385
XceI RCATGY 1 cut(s) 158
XcmI CCANNNNNNNNNTGG 1 cut(s) 185
XspI CTAG 2 cut(s) 239, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.