Rh6AG508900

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002944
Physical Location & Seq
Reverse (-)
68062631 .. 68063462
832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG508900.1

Sequence Viewer

Length: 447 bp
ATGGAGGAGTCTGCAGCTGGTGCCGATGAGGTGGAGATGGTGACGTGTCACTGCTGCGGGTTAGAGGAGGAGTGCACGCCGGGATATATAGCGCGGGAAAGGGAGAAGCACCATGGCCGTTGGATCTGCGGGCTTTGCACGACGGCGATCGAGGAAGATGGGATGAAGTTGCAGAGGAAGATCACCGCCGACGAGGCCATCAGGCGTCACGTCAAGTTTTACGAGAACTTTCGGTCGACGTCTCCGAGGAGGAGTGTGGATTCTTCCGCGGCGGAGGACGAGGAGGAGGAGAAGGACTTGTTGATCTCGAAGGTGATGCAGCTCCTGCGGCGGACTTTGGACTCGCCGAGGAGGACCGGGACGGGTTCCGGTCGCGGGGCGTTTGATCACCGTCTTTTGAGGTCGAAGAGCTGCTTTGCGACTCTAGGAGAGAAGGGTCTAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

148

Amino Acids

16.69

Weight (kDa)

5.79

Isoelectric Point (pI)

77.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 11 - 86 1.7e-25 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 20
AccI GTMKAC 1 cut(s) 236
AccII CGCG 3 cut(s) 94, 269, 375
AclWI GGATC 1 cut(s) 131
AcoI YGGCCR 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 205, 239
AfiI CCNNNNNNNGG 1 cut(s) 375
AflIII ACRYGT 1 cut(s) 44
AjiI CACGTC 2 cut(s) 45, 211
AluBI AGCT 3 cut(s) 17, 322, 411
AluI AGCT 3 cut(s) 17, 322, 411
Alw21I GWGCWC 1 cut(s) 77
Alw26I GTCTC 1 cut(s) 246
Alw44I GTGCAC 1 cut(s) 73
AlwI GGATC 1 cut(s) 131
AlwNI CAGNNNCTG 1 cut(s) 325
AoxI GGCC 2 cut(s) 115, 195
ApaLI GTGCAC 1 cut(s) 73
ApeKI GCWGC 4 cut(s) 14, 54, 319, 411
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 1 cut(s) 354
AsuC2I CCSGG 2 cut(s) 81, 358
AsuHPI GGTGA 4 cut(s) 52, 175, 325, 380
AvaII GGWCC 1 cut(s) 354
BaeGI GKGCMC 1 cut(s) 77
BanI GGYRCC 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 77
BbvI GCAGC 4 cut(s) 26, 41, 331, 398
BccI CCATC 3 cut(s) 31, 152, 206
BceAI ACGGC 2 cut(s) 102, 159
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BclI TGATCA 1 cut(s) 385
BcnI CCSGG 2 cut(s) 81, 358
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 2 cut(s) 425, 440
BfmI CTRYAG 1 cut(s) 12
BglI GCCNNNNNGGC 1 cut(s) 194
BisI GCNGC 6 cut(s) 15, 55, 270, 320, 329, 412
BlsI GCNGC 6 cut(s) 16, 56, 271, 321, 330, 413
Bme1390I CCNGG 2 cut(s) 81, 358
Bme18I GGWCC 1 cut(s) 354
BmgBI CACGTC 2 cut(s) 45, 211
BmgT120I GGNCC 1 cut(s) 354
BmiI GGNNCC 2 cut(s) 22, 367
BmrFI CCNGG 2 cut(s) 81, 358
BmsI GCATC 1 cut(s) 306
BpuMI CCSGG 2 cut(s) 81, 358
BsaHI GRCGYC 2 cut(s) 205, 239
BsaJI CCNNGG 4 cut(s) 112, 245, 267, 347
BsaWI WCCGGW 1 cut(s) 368
Bsc4I CCNNNNNNNGG 1 cut(s) 375
BseDI CCNNGG 4 cut(s) 112, 245, 267, 347
BseGI GGATG 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 375
BseRI GAGGAG 9 cut(s) 20, 80, 83, 262, 265, 296, 299, 302, 364
BseSI GKGCMC 1 cut(s) 77
BseXI GCAGC 4 cut(s) 26, 41, 331, 398
Bsh1236I CGCG 3 cut(s) 94, 269, 375
Bsh1285I CGRYCG 3 cut(s) 150, 236, 373
BshFI GGCC 2 cut(s) 117, 197
BshNI GGYRCC 1 cut(s) 20
BsiEI CGRYCG 3 cut(s) 150, 236, 373
BsiHKAI GWGCWC 1 cut(s) 77
BsiSI CCGG 3 cut(s) 80, 357, 369
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 1 cut(s) 375
BsmAI GTCTC 1 cut(s) 246
BsmBI CGTCTC 1 cut(s) 246
BsmFI GGGAC 1 cut(s) 373
BsnI GGCC 2 cut(s) 117, 197
Bsp1286I GDGCHC 1 cut(s) 77
Bsp143I GATC 5 cut(s) 123, 147, 180, 303, 385
Bsp19I CCATGG 1 cut(s) 112
BspANI GGCC 2 cut(s) 117, 197
BspFNI CGCG 3 cut(s) 94, 269, 375
BspLI GGNNCC 2 cut(s) 22, 367
BspMAI CTGCAG 1 cut(s) 16
BspPI GGATC 1 cut(s) 131
BspQI GCTCTTC 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 20
BssECI CCNNGG 4 cut(s) 112, 245, 267, 347
BssMI GATC 5 cut(s) 123, 147, 180, 303, 385
BssNI GRCGYC 2 cut(s) 205, 239
BssT1I CCWWGG 1 cut(s) 112
Bst4CI ACNGT 1 cut(s) 392
Bst6I CTCTTC 1 cut(s) 401
BstACI GRCGYC 2 cut(s) 205, 239
BstAPI GCANNNNNTGC 2 cut(s) 20, 325
BstC8I GCNNGC 2 cut(s) 77, 131
BstDSI CCRYGG 2 cut(s) 112, 267
BstF5I GGATG 1 cut(s) 168
BstFNI CGCG 3 cut(s) 94, 269, 375
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 5 cut(s) 126, 150, 183, 306, 388
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 5 cut(s) 123, 147, 180, 303, 385
BstMCI CGRYCG 3 cut(s) 150, 236, 373
BstMWI GCNNNNNNNGC 5 cut(s) 20, 135, 194, 325, 328
BstSCI CCNGG 2 cut(s) 79, 356
BstSFI CTRYAG 1 cut(s) 12
BstSLI GKGCMC 1 cut(s) 77
BstUI CGCG 3 cut(s) 94, 269, 375
BstV1I GCAGC 4 cut(s) 26, 41, 331, 398
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 2 cut(s) 117, 197
BtgI CCRYGG 2 cut(s) 112, 267
BtrI CACGTC 2 cut(s) 45, 211
BtsCI GGATG 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 49
Cac8I GCNNGC 2 cut(s) 77, 131
CaiI CAGNNNCTG 1 cut(s) 325
CfoI GCGC 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 354
Cfr42I CCGCGG 1 cut(s) 270
CseI GACGC 1 cut(s) 194
CviAII CATG 1 cut(s) 113
CviJI RGCY 6 cut(s) 17, 117, 133, 197, 322, 411
CviKI_1 RGCY 6 cut(s) 17, 117, 133, 197, 322, 411
DpnI GATC 5 cut(s) 125, 149, 182, 305, 387
DpnII GATC 5 cut(s) 123, 147, 180, 303, 385
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
EciI GGCGGA 2 cut(s) 287, 346
Eco130I CCWWGG 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 112
Esp3I CGTCTC 1 cut(s) 246
FaeI CATG 1 cut(s) 116
FaiI YATR 3 cut(s) 87, 89, 114
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FaqI GGGAC 1 cut(s) 373
FatI CATG 1 cut(s) 112
FauI CCCGC 4 cut(s) 50, 87, 122, 368
FbaI TGATCA 1 cut(s) 385
FblI GTMKAC 1 cut(s) 236
Fnu4HI GCNGC 6 cut(s) 15, 55, 270, 320, 329, 412
FokI GGATG 1 cut(s) 175
Fsp4HI GCNGC 6 cut(s) 15, 55, 270, 320, 329, 412
FspBI CTAG 2 cut(s) 425, 440
GlaI GCGC 1 cut(s) 93
GluI GCNGC 6 cut(s) 15, 55, 270, 320, 329, 412
HaeIII GGCC 2 cut(s) 117, 197
HapII CCGG 3 cut(s) 80, 357, 369
HgaI GACGC 1 cut(s) 194
HhaI GCGC 1 cut(s) 94
Hin1I GRCGYC 2 cut(s) 205, 239
Hin1II CATG 1 cut(s) 116
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HincII GTYRAC 1 cut(s) 237
HindII GTYRAC 1 cut(s) 237
HinfI GANTC 4 cut(s) 8, 260, 341, 421
HpaII CCGG 3 cut(s) 80, 357, 369
HphI GGTGA 4 cut(s) 52, 175, 325, 380
Hpy166II GTNNAC 2 cut(s) 75, 237
Hpy188I TCNGA 1 cut(s) 246
Hpy188III TCNNGA 2 cut(s) 307, 440
Hpy8I GTNNAC 2 cut(s) 75, 237
Hpy99I CGWCG 3 cut(s) 145, 194, 241
HpyAV CCTTC 3 cut(s) 286, 304, 427
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4IV ACGT 3 cut(s) 44, 210, 239
HpyCH4V TGCA 5 cut(s) 14, 75, 138, 172, 319
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 135, 194, 325, 328
HpySE526I ACGT 3 cut(s) 44, 210, 239
Hsp92I GRCGYC 2 cut(s) 205, 239
Hsp92II CATG 1 cut(s) 116
HspAI GCGC 1 cut(s) 92
Ksp22I TGATCA 1 cut(s) 385
KspI CCGCGG 1 cut(s) 270
Kzo9I GATC 5 cut(s) 123, 147, 180, 303, 385
LguI GCTCTTC 1 cut(s) 401
LmnI GCTCC 1 cut(s) 327
LpnPI CCDG 6 cut(s) 3, 93, 187, 338, 370, 382
Lsp1109I GCAGC 4 cut(s) 26, 41, 331, 398
LweI GCATC 1 cut(s) 306
MaeI CTAG 2 cut(s) 425, 440
MaeII ACGT 3 cut(s) 44, 210, 239
MaeIII GTNAC 3 cut(s) 40, 47, 206
MalI GATC 5 cut(s) 125, 149, 182, 305, 387
MboI GATC 5 cut(s) 123, 147, 180, 303, 385
MboII GAAGA 4 cut(s) 167, 190, 255, 418
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 1 cut(s) 77
MlyI GAGTC 3 cut(s) 17, 335, 415
MmeI TCCRAC 1 cut(s) 101
MspA1I CMGCKG 2 cut(s) 17, 269
MspI CCGG 3 cut(s) 80, 357, 369
MspR9I CCNGG 2 cut(s) 81, 358
MvnI CGCG 3 cut(s) 94, 269, 375
MwoI GCNNNNNNNGC 5 cut(s) 20, 135, 194, 325, 328
NciI CCSGG 2 cut(s) 81, 358
NcoI CCATGG 1 cut(s) 112
NdeII GATC 5 cut(s) 123, 147, 180, 303, 385
NlaIII CATG 1 cut(s) 116
NlaIV GGNNCC 2 cut(s) 22, 367
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 3 cut(s) 40, 47, 206
PciSI GCTCTTC 1 cut(s) 401
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 6 cut(s) 16, 56, 271, 321, 330, 413
Ple19I CGATCG 1 cut(s) 150
PleI GAGTC 3 cut(s) 16, 335, 415
PpsI GAGTC 3 cut(s) 16, 335, 415
PspN4I GGNNCC 2 cut(s) 22, 367
PspPI GGNCC 1 cut(s) 354
PstI CTGCAG 1 cut(s) 16
PstNI CAGNNNCTG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 123
PvuI CGATCG 1 cut(s) 150
PvuII CAGCTG 1 cut(s) 17
SacII CCGCGG 1 cut(s) 270
SalI GTCGAC 1 cut(s) 235
SapI GCTCTTC 1 cut(s) 401
SatI GCNGC 6 cut(s) 15, 55, 270, 320, 329, 412
Sau3AI GATC 5 cut(s) 123, 147, 180, 303, 385
Sau96I GGNCC 1 cut(s) 354
SchI GAGTC 3 cut(s) 17, 335, 415
ScrFI CCNGG 2 cut(s) 81, 358
SduI GDGCHC 1 cut(s) 77
SetI ASST 9 cut(s) 19, 33, 47, 213, 242, 315, 324, 404, 413
SfaNI GCATC 1 cut(s) 306
SfcI CTRYAG 1 cut(s) 12
Sfr303I CCGCGG 1 cut(s) 270
SgrBI CCGCGG 1 cut(s) 270
SinI GGWCC 1 cut(s) 354
SspMI CTAG 2 cut(s) 425, 440
StyD4I CCNGG 2 cut(s) 79, 356
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 392
TaiI ACGT 3 cut(s) 47, 213, 242
TaqI TCGA 4 cut(s) 150, 236, 308, 404
TaqII GACCGA 1 cut(s) 222
TauI GCSGC 2 cut(s) 272, 331
TfiI GAWTC 1 cut(s) 260
TscAI CASTG 1 cut(s) 56
TseFI GTSAC 3 cut(s) 40, 47, 206
TseI GCWGC 4 cut(s) 14, 54, 319, 411
Tsp45I GTSAC 3 cut(s) 40, 47, 206
TspDTI ATGAA 1 cut(s) 179
TspRI CASTG 1 cut(s) 56
VneI GTGCAC 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 354
XbaI TCTAGA 1 cut(s) 439
XmiI GTMKAC 1 cut(s) 236
XspI CTAG 2 cut(s) 425, 440
ZraI GACGTC 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.