Rorug06G0392500

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002944
Physical Location & Seq
Reverse (-)
53805083 .. 53806964
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0392500.1

Sequence Viewer

Length: 483 bp
ATGTGGAGGCGAGTGGTGTCTTGTTCGACCTCGGCTCAATTCCAAGCCCTAGGCTCATGCAGATCTTCTCCATCTCTCAACCTCACCCGTCGATTTCTAATCTCCTCAGCATCCACGCCTTCCACTCCCCCGCCGTTTCAGCGATCCCCCAATCCCGCCTCCGGTTTGAAGAAGAAGGTTGAGGATATTATGCCCATTGCTACCGGTCACGAGCGCGAGGAACTTCAGGCTGAGCTTCAGGGGAGGGACGTTCTTGAGATCAATCATCCAGTCGGCCCTTTTGGTACTAAGGAAGCACCTGCTATAGTCAAGTCCTACTATGACAGAAGAATAGTTGGCTGCCCTGGTGTTGAAGGAGAGGATGAGCATGATGTTGTTTGGTTTTGGCTGGAGAAAGGCAAGCCACATGAATGCCCTGTCTGTTCCCAATATTTCAAGCTGGAAGTTGTTGGCCCAGGAGGTCCTCCAGATGGTCATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

160

Amino Acids

17.65

Weight (kDa)

6.59

Isoelectric Point (pI)

64.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COX5B PF01215 67 - 154 4.8e-20 Cytochrome c oxidase subunit Vb
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 307
Acc36I ACCTGC 1 cut(s) 307
AccII CGCG 1 cut(s) 216
AciI CCGC 2 cut(s) 131, 156
AclWI GGATC 1 cut(s) 138
AcuI CTGAAG 2 cut(s) 209, 221
AfaI GTAC 1 cut(s) 286
AfiI CCNNNNNNNGG 2 cut(s) 161, 470
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 3 cut(s) 169, 353, 436
AjnI CCWGG 2 cut(s) 343, 454
AluBI AGCT 2 cut(s) 235, 439
AluI AGCT 2 cut(s) 235, 439
AlwI GGATC 1 cut(s) 138
AoxI GGCC 2 cut(s) 274, 451
ApeKI GCWGC 1 cut(s) 339
AsiGI ACCGGT 1 cut(s) 203
AspA2I CCTAGG 1 cut(s) 49
AspLEI GCGC 1 cut(s) 216
AspS9I GGNCC 3 cut(s) 275, 452, 461
AsuHPI GGTGA 1 cut(s) 76
AvaII GGWCC 1 cut(s) 461
AvrII CCTAGG 1 cut(s) 49
BauI CACGAG 1 cut(s) 209
BbvCI CCTCAGC 1 cut(s) 106
BbvI GCAGC 1 cut(s) 326
BccI CCATC 2 cut(s) 79, 464
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 2 cut(s) 345, 456
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 303
BfuAI ACCTGC 1 cut(s) 307
BglII AGATCT 1 cut(s) 62
BisI GCNGC 1 cut(s) 340
BlnI CCTAGG 1 cut(s) 49
BlpI GCTNAGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 341
Bme1390I CCNGG 2 cut(s) 345, 456
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 3 cut(s) 275, 452, 461
BmrFI CCNGG 2 cut(s) 345, 456
BmsI GCATC 1 cut(s) 119
BpmI CTGGAG 2 cut(s) 410, 450
Bpu10I CCTNAGC 1 cut(s) 106
Bpu1102I GCTNAGC 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 275
BsaBI GATNNNNATC 1 cut(s) 474
BsaJI CCNNGG 4 cut(s) 30, 49, 343, 454
BsaWI WCCGGW 2 cut(s) 161, 203
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 470
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 269
Bse3DI GCAATG 1 cut(s) 195
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 2 cut(s) 345, 456
BseDI CCNNGG 4 cut(s) 30, 49, 343, 454
BseGI GGATG 3 cut(s) 110, 265, 367
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 2 cut(s) 161, 470
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 2 cut(s) 120, 222
BseNI ACTGG 1 cut(s) 269
BseRI GAGGAG 1 cut(s) 94
BseXI GCAGC 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 216
BshFI GGCC 2 cut(s) 276, 453
BshTI ACCGGT 1 cut(s) 203
BsiSI CCGG 2 cut(s) 162, 204
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 2 cut(s) 161, 470
BsmFI GGGAC 1 cut(s) 260
BsmI GAATGC 1 cut(s) 416
BsnI GGCC 2 cut(s) 276, 453
Bsp143I GATC 3 cut(s) 62, 143, 258
Bsp1720I GCTNAGC 1 cut(s) 231
BspACI CCGC 2 cut(s) 131, 156
BspANI GGCC 2 cut(s) 276, 453
BspCNI CTCAG 2 cut(s) 119, 223
BspFNI CGCG 1 cut(s) 216
BspMI ACCTGC 1 cut(s) 307
BspPI GGATC 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 195
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 1 cut(s) 269
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 4 cut(s) 30, 49, 343, 454
BssMI GATC 3 cut(s) 62, 143, 258
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 49
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 2 cut(s) 345, 456
BstC8I GCNNGC 1 cut(s) 401
BstDEI CTNAG 3 cut(s) 106, 231, 288
BstF5I GGATG 3 cut(s) 110, 265, 367
BstFNI CGCG 1 cut(s) 216
BstHHI GCGC 1 cut(s) 216
BstKTI GATC 3 cut(s) 65, 146, 261
BstMBI GATC 3 cut(s) 62, 143, 258
BstMWI GCNNNNNNNGC 1 cut(s) 139
BstNI CCWGG 2 cut(s) 345, 456
BstSCI CCNGG 2 cut(s) 343, 454
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 326
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BsuRI GGCC 2 cut(s) 276, 453
BtsCI GGATG 3 cut(s) 110, 265, 367
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 1 cut(s) 401
CfoI GCGC 1 cut(s) 216
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 3 cut(s) 275, 452, 461
Csp6I GTAC 1 cut(s) 285
CspAI ACCGGT 1 cut(s) 203
CviAII CATG 3 cut(s) 57, 368, 407
CviQI GTAC 1 cut(s) 285
DdeI CTNAG 3 cut(s) 106, 231, 288
DpnI GATC 3 cut(s) 64, 145, 260
DpnII GATC 3 cut(s) 62, 143, 258
Eco130I CCWWGG 1 cut(s) 49
Eco47I GGWCC 1 cut(s) 461
Eco57I CTGAAG 2 cut(s) 209, 221
EcoO109I RGGNCCY 1 cut(s) 461
EcoRII CCWGG 2 cut(s) 343, 454
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 3 cut(s) 60, 371, 410
FaiI YATR 6 cut(s) 58, 191, 305, 321, 369, 408
FaqI GGGAC 1 cut(s) 260
FatI CATG 3 cut(s) 56, 367, 406
FauI CCCGC 2 cut(s) 138, 163
Fnu4HI GCNGC 1 cut(s) 340
FokI GGATG 3 cut(s) 97, 252, 374
Fsp4HI GCNGC 1 cut(s) 340
FspBI CTAG 1 cut(s) 50
GlaI GCGC 1 cut(s) 215
GluI GCNGC 1 cut(s) 340
GsuI CTGGAG 2 cut(s) 410, 450
HaeIII GGCC 2 cut(s) 276, 453
HapII CCGG 2 cut(s) 162, 204
HhaI GCGC 1 cut(s) 216
Hin1II CATG 3 cut(s) 60, 371, 410
Hin6I GCGC 1 cut(s) 214
HinP1I GCGC 1 cut(s) 214
HpaII CCGG 2 cut(s) 162, 204
HphI GGTGA 1 cut(s) 76
Hpy188III TCNNGA 3 cut(s) 209, 254, 467
Hpy99I CGWCG 1 cut(s) 93
HpyAV CCTTC 3 cut(s) 129, 169, 347
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 1 cut(s) 60
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
HpyF3I CTNAG 3 cut(s) 106, 231, 288
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 3 cut(s) 60, 371, 410
HspAI GCGC 1 cut(s) 214
Kzo9I GATC 3 cut(s) 62, 143, 258
Lsp1109I GCAGC 1 cut(s) 326
LweI GCATC 1 cut(s) 119
MaeI CTAG 1 cut(s) 50
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 206
MalI GATC 3 cut(s) 64, 145, 260
MboI GATC 3 cut(s) 62, 143, 258
MboII GAAGA 4 cut(s) 57, 181, 184, 339
MflI RGATCY 1 cut(s) 62
MluCI AATT 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 409
MspI CCGG 2 cut(s) 162, 204
MspR9I CCNGG 2 cut(s) 345, 456
Mva1269I GAATGC 1 cut(s) 416
MvaI CCWGG 2 cut(s) 345, 456
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 1 cut(s) 139
NdeII GATC 3 cut(s) 62, 143, 258
NlaIII CATG 3 cut(s) 60, 371, 410
NmeAIII GCCGAG 1 cut(s) 11
NmuCI GTSAC 1 cut(s) 206
PaqCI CACCTGC 1 cut(s) 307
PctI GAATGC 1 cut(s) 416
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 1 cut(s) 341
PpuMI RGGWCCY 1 cut(s) 461
Psp5II RGGWCCY 1 cut(s) 461
Psp6I CCWGG 2 cut(s) 343, 454
PspGI CCWGG 2 cut(s) 343, 454
PspPI GGNCC 3 cut(s) 275, 452, 461
PspPPI RGGWCCY 1 cut(s) 461
PsuI RGATCY 1 cut(s) 62
RsaI GTAC 1 cut(s) 286
RsaNI GTAC 1 cut(s) 285
RseI CAYNNNNRTG 1 cut(s) 409
SatI GCNGC 1 cut(s) 340
Sau3AI GATC 3 cut(s) 62, 143, 258
Sau96I GGNCC 3 cut(s) 275, 452, 461
ScrFI CCNGG 2 cut(s) 345, 456
SetI ASST 8 cut(s) 32, 84, 180, 237, 252, 301, 441, 463
SfaNI GCATC 1 cut(s) 119
SfcI CTRYAG 1 cut(s) 303
SinI GGWCC 1 cut(s) 461
SmiMI CAYNNNNRTG 1 cut(s) 409
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 1 cut(s) 38
SsiI CCGC 2 cut(s) 131, 156
SspI AATATT 1 cut(s) 431
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 2 cut(s) 343, 454
StyI CCWWGG 1 cut(s) 49
TaiI ACGT 1 cut(s) 252
TaqI TCGA 2 cut(s) 26, 91
TasI AATT 1 cut(s) 38
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 1 cut(s) 339
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 1 cut(s) 423
VpaK11BI GGWCC 1 cut(s) 461
XmaJI CCTAGG 1 cut(s) 49
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.