Rmu_sc0005381.1_g000011

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005381.1
OG0002944
Physical Location & Seq
Reverse (-)
69018 .. 69461
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005381.1_g000011.1.cds

Sequence Viewer

Length: 444 bp
atggaggagtctgcagctggtgccgatgaggtggagatggtgacgtgtcactgctgcgggttagaggaggagtgcacgccaggatatatagcgcgggaaagggagaagcaccatggccgttggatctgcggcctttgcacgacggcgatcgaggacgataggatgaagttgcagaggaagatcaccgccgacgaggctatcaggcgccacgtcaagttttacgagaactttcggtcgacgtctccgaggaggagtgtagattcttccgcggcggaggaggaggaggagaaggacttgttgatctcgaaggtgatgcagctcctgcggcggactttggactcgccgaggaggaccgggacgggttccggtcgcggggcgtttgatcaccgtcttttgaggtcgaagagctgctttgcgactgtaggagagaagggtctagactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

147

Amino Acids

16.65

Weight (kDa)

6.23

Isoelectric Point (pI)

75.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 242
AccB1I GGYRCC 2 cut(s) 20, 204
AccI GTMKAC 1 cut(s) 236
AccII CGCG 3 cut(s) 94, 269, 372
AclWI GGATC 1 cut(s) 131
AcoI YGGCCR 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 205, 239
AfiI CCNNNNNNNGG 1 cut(s) 372
AflIII ACRYGT 1 cut(s) 44
AjiI CACGTC 2 cut(s) 45, 211
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 3 cut(s) 17, 319, 408
AluI AGCT 3 cut(s) 17, 319, 408
Alw21I GWGCWC 1 cut(s) 77
Alw26I GTCTC 1 cut(s) 246
Alw44I GTGCAC 1 cut(s) 73
AlwI GGATC 1 cut(s) 131
AlwNI CAGNNNCTG 1 cut(s) 322
AoxI GGCC 2 cut(s) 115, 130
ApaLI GTGCAC 1 cut(s) 73
ApeKI GCWGC 4 cut(s) 14, 54, 316, 408
AspLEI GCGC 2 cut(s) 94, 207
AspS9I GGNCC 1 cut(s) 351
AsuC2I CCSGG 1 cut(s) 355
AsuHPI GGTGA 4 cut(s) 52, 175, 322, 377
AvaII GGWCC 1 cut(s) 351
BaeGI GKGCMC 1 cut(s) 77
BanI GGYRCC 2 cut(s) 20, 204
Bbv12I GWGCWC 1 cut(s) 77
BbvI GCAGC 4 cut(s) 26, 41, 328, 395
BccI CCATC 1 cut(s) 31
BceAI ACGGC 2 cut(s) 102, 159
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 1 cut(s) 81
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 1 cut(s) 437
BfmI CTRYAG 2 cut(s) 12, 420
BfoI RGCGCY 1 cut(s) 208
BglI GCCNNNNNGGC 1 cut(s) 194
BisI GCNGC 7 cut(s) 15, 55, 130, 270, 317, 326, 409
BlsI GCNGC 7 cut(s) 16, 56, 131, 271, 318, 327, 410
Bme1390I CCNGG 2 cut(s) 81, 355
Bme18I GGWCC 1 cut(s) 351
BmgBI CACGTC 2 cut(s) 45, 211
BmgT120I GGNCC 1 cut(s) 351
BmiI GGNNCC 3 cut(s) 22, 206, 364
BmrFI CCNGG 2 cut(s) 81, 355
BmsI GCATC 1 cut(s) 303
BpuMI CCSGG 1 cut(s) 355
BsaHI GRCGYC 2 cut(s) 205, 239
BsaJI CCNNGG 4 cut(s) 112, 245, 267, 344
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 1 cut(s) 372
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 4 cut(s) 112, 245, 267, 344
BseGI GGATG 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 372
BseSI GKGCMC 1 cut(s) 77
BseXI GCAGC 4 cut(s) 26, 41, 328, 395
Bsh1236I CGCG 3 cut(s) 94, 269, 372
Bsh1285I CGRYCG 3 cut(s) 150, 236, 370
BshFI GGCC 2 cut(s) 117, 132
BshNI GGYRCC 2 cut(s) 20, 204
BsiEI CGRYCG 3 cut(s) 150, 236, 370
BsiHKAI GWGCWC 1 cut(s) 77
BsiSI CCGG 2 cut(s) 354, 366
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 1 cut(s) 372
BsmAI GTCTC 1 cut(s) 246
BsmBI CGTCTC 1 cut(s) 246
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 2 cut(s) 117, 132
Bsp1286I GDGCHC 1 cut(s) 77
Bsp143I GATC 5 cut(s) 123, 147, 180, 300, 382
Bsp19I CCATGG 1 cut(s) 112
BspANI GGCC 2 cut(s) 117, 132
BspFNI CGCG 3 cut(s) 94, 269, 372
BspLI GGNNCC 3 cut(s) 22, 206, 364
BspMAI CTGCAG 1 cut(s) 16
BspPI GGATC 1 cut(s) 131
BspQI GCTCTTC 1 cut(s) 398
BspT107I GGYRCC 2 cut(s) 20, 204
BssECI CCNNGG 4 cut(s) 112, 245, 267, 344
BssMI GATC 5 cut(s) 123, 147, 180, 300, 382
BssNI GRCGYC 2 cut(s) 205, 239
BssT1I CCWWGG 1 cut(s) 112
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 2 cut(s) 389, 421
Bst6I CTCTTC 1 cut(s) 398
BstACI GRCGYC 2 cut(s) 205, 239
BstAPI GCANNNNNTGC 2 cut(s) 20, 322
BstC8I GCNNGC 1 cut(s) 77
BstDSI CCRYGG 2 cut(s) 112, 267
BstF5I GGATG 1 cut(s) 168
BstFNI CGCG 3 cut(s) 94, 269, 372
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 2 cut(s) 94, 207
BstKTI GATC 5 cut(s) 126, 150, 183, 303, 385
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 5 cut(s) 123, 147, 180, 300, 382
BstMCI CGRYCG 3 cut(s) 150, 236, 370
BstMWI GCNNNNNNNGC 5 cut(s) 20, 135, 194, 322, 325
BstNI CCWGG 1 cut(s) 81
BstSCI CCNGG 2 cut(s) 79, 353
BstSFI CTRYAG 2 cut(s) 12, 420
BstSLI GKGCMC 1 cut(s) 77
BstUI CGCG 3 cut(s) 94, 269, 372
BstV1I GCAGC 4 cut(s) 26, 41, 328, 395
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 2 cut(s) 117, 132
BtgI CCRYGG 2 cut(s) 112, 267
BtrI CACGTC 2 cut(s) 45, 211
BtsCI GGATG 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 49
Cac8I GCNNGC 1 cut(s) 77
CaiI CAGNNNCTG 1 cut(s) 322
CfoI GCGC 2 cut(s) 94, 207
Cfr13I GGNCC 1 cut(s) 351
Cfr42I CCGCGG 1 cut(s) 270
CviAII CATG 1 cut(s) 113
CviJI RGCY 6 cut(s) 17, 117, 132, 197, 319, 408
CviKI_1 RGCY 6 cut(s) 17, 117, 132, 197, 319, 408
DinI GGCGCC 1 cut(s) 206
DpnI GATC 5 cut(s) 125, 149, 182, 302, 384
DpnII GATC 5 cut(s) 123, 147, 180, 300, 382
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
EciI GGCGGA 2 cut(s) 287, 343
Eco130I CCWWGG 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 351
EcoRII CCWGG 1 cut(s) 79
EcoT14I CCWWGG 1 cut(s) 112
EgeI GGCGCC 1 cut(s) 206
EheI GGCGCC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 112
Esp3I CGTCTC 1 cut(s) 246
FaeI CATG 1 cut(s) 116
FaiI YATR 3 cut(s) 87, 89, 114
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 1 cut(s) 370
FatI CATG 1 cut(s) 112
FauI CCCGC 3 cut(s) 50, 87, 365
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 1 cut(s) 236
Fnu4HI GCNGC 7 cut(s) 15, 55, 130, 270, 317, 326, 409
FokI GGATG 1 cut(s) 175
Fsp4HI GCNGC 7 cut(s) 15, 55, 130, 270, 317, 326, 409
FspBI CTAG 1 cut(s) 437
GlaI GCGC 2 cut(s) 93, 206
GluI GCNGC 7 cut(s) 15, 55, 130, 270, 317, 326, 409
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 2 cut(s) 117, 132
HapII CCGG 2 cut(s) 354, 366
HhaI GCGC 2 cut(s) 94, 207
Hin1I GRCGYC 2 cut(s) 205, 239
Hin1II CATG 1 cut(s) 116
Hin6I GCGC 2 cut(s) 92, 205
HinP1I GCGC 2 cut(s) 92, 205
HincII GTYRAC 1 cut(s) 237
HindII GTYRAC 1 cut(s) 237
HinfI GANTC 3 cut(s) 8, 260, 338
HpaII CCGG 2 cut(s) 354, 366
HphI GGTGA 4 cut(s) 52, 175, 322, 377
Hpy166II GTNNAC 2 cut(s) 75, 237
Hpy188I TCNGA 1 cut(s) 246
Hpy188III TCNNGA 2 cut(s) 304, 437
Hpy8I GTNNAC 2 cut(s) 75, 237
Hpy99I CGWCG 3 cut(s) 145, 194, 241
HpyAV CCTTC 3 cut(s) 283, 301, 424
HpyCH4III ACNGT 2 cut(s) 389, 421
HpyCH4IV ACGT 3 cut(s) 44, 210, 239
HpyCH4V TGCA 5 cut(s) 14, 75, 138, 172, 316
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 135, 194, 322, 325
HpySE526I ACGT 3 cut(s) 44, 210, 239
Hsp92I GRCGYC 2 cut(s) 205, 239
Hsp92II CATG 1 cut(s) 116
HspAI GCGC 2 cut(s) 92, 205
KasI GGCGCC 1 cut(s) 204
Ksp22I TGATCA 1 cut(s) 382
KspI CCGCGG 1 cut(s) 270
Kzo9I GATC 5 cut(s) 123, 147, 180, 300, 382
LguI GCTCTTC 1 cut(s) 398
LmnI GCTCC 1 cut(s) 324
LpnPI CCDG 7 cut(s) 3, 66, 93, 187, 335, 367, 379
Lsp1109I GCAGC 4 cut(s) 26, 41, 328, 395
LweI GCATC 1 cut(s) 303
MaeI CTAG 1 cut(s) 437
MaeII ACGT 3 cut(s) 44, 210, 239
MaeIII GTNAC 2 cut(s) 40, 47
MalI GATC 5 cut(s) 125, 149, 182, 302, 384
MboI GATC 5 cut(s) 123, 147, 180, 300, 382
MboII GAAGA 3 cut(s) 190, 255, 415
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 1 cut(s) 77
Mly113I GGCGCC 1 cut(s) 205
MlyI GAGTC 2 cut(s) 17, 332
MmeI TCCRAC 1 cut(s) 101
MspA1I CMGCKG 2 cut(s) 17, 269
MspI CCGG 2 cut(s) 354, 366
MspR9I CCNGG 2 cut(s) 81, 355
MvaI CCWGG 1 cut(s) 81
MvnI CGCG 3 cut(s) 94, 269, 372
MwoI GCNNNNNNNGC 5 cut(s) 20, 135, 194, 322, 325
NarI GGCGCC 1 cut(s) 205
NciI CCSGG 1 cut(s) 355
NcoI CCATGG 1 cut(s) 112
NdeII GATC 5 cut(s) 123, 147, 180, 300, 382
NlaIII CATG 1 cut(s) 116
NlaIV GGNNCC 3 cut(s) 22, 206, 364
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 2 cut(s) 40, 47
PciSI GCTCTTC 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 7 cut(s) 16, 56, 131, 271, 318, 327, 410
Ple19I CGATCG 1 cut(s) 150
PleI GAGTC 2 cut(s) 16, 332
PluTI GGCGCC 1 cut(s) 208
PpsI GAGTC 2 cut(s) 16, 332
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 3 cut(s) 22, 206, 364
PspPI GGNCC 1 cut(s) 351
PstI CTGCAG 1 cut(s) 16
PstNI CAGNNNCTG 1 cut(s) 322
PsuI RGATCY 1 cut(s) 123
PvuI CGATCG 1 cut(s) 150
PvuII CAGCTG 1 cut(s) 17
SacII CCGCGG 1 cut(s) 270
SalI GTCGAC 1 cut(s) 235
SapI GCTCTTC 1 cut(s) 398
SatI GCNGC 7 cut(s) 15, 55, 130, 270, 317, 326, 409
Sau3AI GATC 5 cut(s) 123, 147, 180, 300, 382
Sau96I GGNCC 1 cut(s) 351
SchI GAGTC 2 cut(s) 17, 332
ScrFI CCNGG 2 cut(s) 81, 355
SduI GDGCHC 1 cut(s) 77
SetI ASST 9 cut(s) 19, 33, 47, 213, 242, 312, 321, 401, 410
SfaNI GCATC 1 cut(s) 303
SfcI CTRYAG 2 cut(s) 12, 420
SfoI GGCGCC 1 cut(s) 206
Sfr303I CCGCGG 1 cut(s) 270
SgrBI CCGCGG 1 cut(s) 270
SinI GGWCC 1 cut(s) 351
SspDI GGCGCC 1 cut(s) 204
SspMI CTAG 1 cut(s) 437
StyD4I CCNGG 2 cut(s) 79, 353
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 2 cut(s) 389, 421
TaiI ACGT 3 cut(s) 47, 213, 242
TaqI TCGA 4 cut(s) 150, 236, 305, 401
TaqII GACCGA 1 cut(s) 222
TauI GCSGC 3 cut(s) 132, 272, 328
TfiI GAWTC 1 cut(s) 260
TscAI CASTG 1 cut(s) 56
TseFI GTSAC 2 cut(s) 40, 47
TseI GCWGC 4 cut(s) 14, 54, 316, 408
Tsp45I GTSAC 2 cut(s) 40, 47
TspDTI ATGAA 1 cut(s) 179
TspRI CASTG 1 cut(s) 56
VneI GTGCAC 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 351
XbaI TCTAGA 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 236
XspI CTAG 1 cut(s) 437
ZraI GACGTC 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.