FvH4_6g35020

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002944
Physical Location & Seq
Reverse (-)
27677558 .. 27678037
480 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35020.t1

Sequence Viewer

Length: 480 bp
ATGTATGCAACTATAATCAGTGAACCAGTGGGAGGTCTACCAGCCCCAGAAAGTCAAGCAGCAGCAGCCCCAAAGCTCAATCCCCAATCAGAGATTGAGTCTGCAAAGTGTGAATGCTGTGGGCTCACAGAGGAGTGCACCCCTGCCTATATTGAACATGTCCGGGAGAGAAACATGGGCCATTGGATTTGTGGGTTGTGTTCTGAGGCTATTAAAGATGAGATTGTGAGGTCTGAGAGGCTACTTAGCACTGAAGAAGCCATGGCTACACATATGAACTTCTGCATGAAGTTTAAGGCATCCGGCCCTCCTCTGAACCCTACTATTCATTTGATATCTGCCATGACAAAGATTCTCCGGCGCAGTTTGGACTCTCCGAGGGGCTCGAGTTCGACTCCGAGTACTCCCATGAGGATGGGTGGCCGCCCGGGGCTTACCAGGTCCGGGAGTTGCTTTCCTGCCCTGACCGGCGTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

160

Amino Acids

17.12

Weight (kDa)

6.43

Isoelectric Point (pI)

83.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 32 - 125 1.1e-39 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 37
AciI CCGC 1 cut(s) 424
AcoI YGGCCR 1 cut(s) 421
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 1 cut(s) 403
AfiI CCNNNNNNNGG 2 cut(s) 32, 444
AflIII ACRYGT 1 cut(s) 157
AgsI TTSAA 1 cut(s) 155
AjnI CCWGG 1 cut(s) 437
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw21I GWGCWC 1 cut(s) 140
Alw44I GTGCAC 1 cut(s) 136
Ama87I CYCGRG 2 cut(s) 385, 427
AoxI GGCC 3 cut(s) 178, 304, 421
ApaLI GTGCAC 1 cut(s) 136
ApeKI GCWGC 3 cut(s) 59, 62, 65
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 3 cut(s) 178, 305, 441
AsuC2I CCSGG 4 cut(s) 164, 428, 429, 445
AvaI CYCGRG 2 cut(s) 385, 427
AvaII GGWCC 1 cut(s) 441
BaeGI GKGCMC 1 cut(s) 140
BanII GRGCYC 2 cut(s) 126, 386
Bbv12I GWGCWC 1 cut(s) 140
BbvI GCAGC 3 cut(s) 71, 74, 77
BccI CCATC 1 cut(s) 409
BciT130I CCWGG 1 cut(s) 439
BcnI CCSGG 4 cut(s) 164, 428, 429, 445
BisI GCNGC 4 cut(s) 60, 63, 66, 424
BlsI GCNGC 4 cut(s) 61, 64, 67, 425
BmcAI AGTACT 1 cut(s) 403
Bme1390I CCNGG 5 cut(s) 164, 428, 429, 439, 445
Bme18I GGWCC 1 cut(s) 441
BmeT110I CYCGRG 2 cut(s) 385, 427
BmgT120I GGNCC 3 cut(s) 178, 305, 441
BmrFI CCNGG 5 cut(s) 164, 428, 429, 439, 445
BmsI GCATC 1 cut(s) 308
BplI GAGNNNNNCTC 2 cut(s) 379, 411
BpuMI CCSGG 4 cut(s) 164, 428, 429, 445
BsaJI CCNNGG 4 cut(s) 261, 377, 427, 428
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 444
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 1 cut(s) 26
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 4 cut(s) 261, 377, 427, 428
BseGI GGATG 2 cut(s) 299, 420
BseLI CCNNNNNNNGG 2 cut(s) 32, 444
BseMII CTCAG 2 cut(s) 195, 225
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 2 cut(s) 146, 300
BseSI GKGCMC 1 cut(s) 140
BseXI GCAGC 3 cut(s) 71, 74, 77
BshFI GGCC 3 cut(s) 180, 306, 423
BsiHKAI GWGCWC 1 cut(s) 140
BsiHKCI CYCGRG 2 cut(s) 385, 427
BsiSI CCGG 6 cut(s) 163, 303, 358, 428, 444, 468
BslI CCNNNNNNNGG 2 cut(s) 32, 444
BsmI GAATGC 1 cut(s) 119
BsnI GGCC 3 cut(s) 180, 306, 423
BsoBI CYCGRG 2 cut(s) 385, 427
Bsp1286I GDGCHC 3 cut(s) 126, 140, 386
Bsp19I CCATGG 1 cut(s) 261
BspACI CCGC 1 cut(s) 424
BspANI GGCC 3 cut(s) 180, 306, 423
BspCNI CTCAG 2 cut(s) 196, 226
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 1 cut(s) 26
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 4 cut(s) 261, 377, 427, 428
BssT1I CCWWGG 1 cut(s) 261
Bst2UI CCWGG 1 cut(s) 439
BstDEI CTNAG 3 cut(s) 204, 234, 245
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 2 cut(s) 299, 420
BstHHI GCGC 1 cut(s) 363
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNI CCWGG 1 cut(s) 439
BstNSI RCATGY 1 cut(s) 161
BstSCI CCNGG 5 cut(s) 162, 426, 427, 437, 443
BstSLI GKGCMC 1 cut(s) 140
BstV1I GCAGC 3 cut(s) 71, 74, 77
BstXI CCANNNNNNTGG 1 cut(s) 415
BsuRI GGCC 3 cut(s) 180, 306, 423
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 2 cut(s) 299, 420
BtsIMutI CAGTG 3 cut(s) 25, 33, 249
CfoI GCGC 1 cut(s) 363
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 3 cut(s) 178, 305, 441
Cfr9I CCCGGG 1 cut(s) 427
CsiI ACCWGGT 1 cut(s) 437
Csp6I GTAC 1 cut(s) 402
CviAII CATG 6 cut(s) 158, 175, 262, 286, 343, 409
CviQI GTAC 1 cut(s) 402
DdeI CTNAG 3 cut(s) 204, 234, 245
EaeI YGGCCR 1 cut(s) 421
Eco130I CCWWGG 1 cut(s) 261
Eco24I GRGCYC 2 cut(s) 126, 386
Eco32I GATATC 1 cut(s) 336
Eco47I GGWCC 1 cut(s) 441
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 2 cut(s) 385, 427
EcoRII CCWGG 1 cut(s) 437
EcoRV GATATC 1 cut(s) 336
EcoT14I CCWWGG 1 cut(s) 261
EcoT38I GRGCYC 2 cut(s) 126, 386
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 6 cut(s) 161, 178, 265, 289, 346, 412
FatI CATG 6 cut(s) 157, 174, 261, 285, 342, 408
FauNDI CATATG 1 cut(s) 273
FblI GTMKAC 1 cut(s) 37
Fnu4HI GCNGC 4 cut(s) 60, 63, 66, 424
FokI GGATG 2 cut(s) 286, 427
FriOI GRGCYC 2 cut(s) 126, 386
Fsp4HI GCNGC 4 cut(s) 60, 63, 66, 424
GlaI GCGC 1 cut(s) 362
GluI GCNGC 4 cut(s) 60, 63, 66, 424
HaeIII GGCC 3 cut(s) 180, 306, 423
HapII CCGG 6 cut(s) 163, 303, 358, 428, 444, 468
HhaI GCGC 1 cut(s) 363
Hin1II CATG 6 cut(s) 161, 178, 265, 289, 346, 412
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HinfI GANTC 4 cut(s) 98, 352, 371, 394
HpaII CCGG 6 cut(s) 163, 303, 358, 428, 444, 468
Hpy166II GTNNAC 3 cut(s) 23, 38, 138
Hpy188I TCNGA 6 cut(s) 91, 205, 235, 315, 378, 399
Hpy8I GTNNAC 3 cut(s) 23, 38, 138
HpyCH4V TGCA 4 cut(s) 8, 104, 138, 285
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 3 cut(s) 204, 234, 245
Hsp92II CATG 6 cut(s) 161, 178, 265, 289, 346, 412
HspAI GCGC 1 cut(s) 361
Lsp1109I GCAGC 3 cut(s) 71, 74, 77
LweI GCATC 1 cut(s) 308
MabI ACCWGGT 1 cut(s) 437
MboII GAAGA 1 cut(s) 266
MhlI GDGCHC 3 cut(s) 126, 140, 386
MlyI GAGTC 3 cut(s) 107, 365, 388
MnlI CCTC 9 cut(s) 26, 124, 199, 222, 231, 318, 321, 372, 405
MseI TTAA 2 cut(s) 213, 294
MslI CAYNNNNRTG 1 cut(s) 413
MspI CCGG 6 cut(s) 163, 303, 358, 428, 444, 468
MspR9I CCNGG 5 cut(s) 164, 428, 429, 439, 445
Mva1269I GAATGC 1 cut(s) 119
MvaI CCWGG 1 cut(s) 439
MwoI GCNNNNNNNGC 1 cut(s) 65
NciI CCSGG 4 cut(s) 164, 428, 429, 445
NcoI CCATGG 1 cut(s) 261
NdeI CATATG 1 cut(s) 273
NlaIII CATG 6 cut(s) 161, 178, 265, 289, 346, 412
NspI RCATGY 1 cut(s) 161
PaeR7I CTCGAG 1 cut(s) 385
PciI ACATGT 1 cut(s) 157
PctI GAATGC 1 cut(s) 119
PfeI GAWTC 1 cut(s) 352
PfoI TCCNGGA 2 cut(s) 162, 443
PkrI GCNGC 4 cut(s) 61, 64, 67, 425
PleI GAGTC 3 cut(s) 106, 365, 388
PpsI GAGTC 3 cut(s) 106, 365, 388
PscI ACATGT 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 437
PspGI CCWGG 1 cut(s) 437
PspPI GGNCC 3 cut(s) 178, 305, 441
PspXI VCTCGAGB 1 cut(s) 385
RsaI GTAC 1 cut(s) 403
RsaNI GTAC 1 cut(s) 402
RseI CAYNNNNRTG 1 cut(s) 413
SaqAI TTAA 2 cut(s) 213, 294
SatI GCNGC 4 cut(s) 60, 63, 66, 424
Sau96I GGNCC 3 cut(s) 178, 305, 441
ScaI AGTACT 1 cut(s) 403
SchI GAGTC 3 cut(s) 107, 365, 388
ScrFI CCNGG 5 cut(s) 164, 428, 429, 439, 445
SduI GDGCHC 3 cut(s) 126, 140, 386
SetI ASST 4 cut(s) 37, 78, 233, 443
SexAI ACCWGGT 1 cut(s) 437
SfaNI GCATC 1 cut(s) 308
Sfr274I CTCGAG 1 cut(s) 385
SinI GGWCC 1 cut(s) 441
SlaI CTCGAG 1 cut(s) 385
SmaI CCCGGG 1 cut(s) 429
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
SsiI CCGC 1 cut(s) 424
StyD4I CCNGG 5 cut(s) 162, 426, 427, 437, 443
StyI CCWWGG 1 cut(s) 261
TaqI TCGA 2 cut(s) 386, 392
TatI WGTACW 1 cut(s) 401
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 1 cut(s) 352
Tru1I TTAA 2 cut(s) 213, 294
Tru9I TTAA 2 cut(s) 213, 294
TscAI CASTG 3 cut(s) 25, 33, 256
TseI GCWGC 3 cut(s) 59, 62, 65
TspDTI ATGAA 3 cut(s) 290, 302, 317
TspMI CCCGGG 1 cut(s) 427
TspRI CASTG 3 cut(s) 25, 33, 256
VneI GTGCAC 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 441
XceI RCATGY 1 cut(s) 161
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
XhoI CTCGAG 1 cut(s) 385
XmaI CCCGGG 1 cut(s) 427
XmiI GTMKAC 1 cut(s) 37
ZrmI AGTACT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.