RLG00000010358

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002944
Physical Location & Seq
Forward (+)
968294 .. 968749
456 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010358

Sequence Viewer

Length: 456 bp
ATGGAGCCCGTGGAGGAGTCTGCAGCTGGTGCCGATGAGGTGGAGATGGTGACGTGTCACTGCTGCGGGTTAGAGGAGGAGTGCACGCCGGGATATATAGCGCGGGAAAGGGAGAAGCACCATGGCCATTGGATCTGCGGGCTTTGCGCGACGGCGATCGAGGACGATAGGATGAAGTTGCAGAGGAAGATCACCGCCGACGAGGCCATCAGGCGCCACGTCAAGTTTTACGAGCACTTTCGGTCGACGTCTCCGAGGAGTAGTGTGGATTCTTCCGCGGCGGAGGAGGAGGAGGAGGAGAAGGACTTGTTGATCTCGAAGGTGATGCAGCTCCTGCGGCGGACTTTGGACTCGCCCAGGAGGACCGGGACGGGTTCCGGTCGCGGGGCGTTTGATCACCGTCTTTTGAGGTCGAAGAGCTGCTTTGCGACTGTAGGAGAGAATTACAGGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

152

Amino Acids

17.13

Weight (kDa)

5.82

Isoelectric Point (pI)

69.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 14 - 89 1.7e-25 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 251
AccB1I GGYRCC 2 cut(s) 29, 213
AccI GTMKAC 1 cut(s) 245
AccII CGCG 4 cut(s) 103, 149, 278, 384
AclWI GGATC 1 cut(s) 140
AcoI YGGCCR 1 cut(s) 124
AcyI GRCGYC 2 cut(s) 214, 248
AfiI CCNNNNNNNGG 1 cut(s) 384
AflIII ACRYGT 1 cut(s) 53
AjiI CACGTC 2 cut(s) 54, 220
AjnI CCWGG 1 cut(s) 356
AluBI AGCT 3 cut(s) 26, 331, 420
AluI AGCT 3 cut(s) 26, 331, 420
Alw21I GWGCWC 2 cut(s) 86, 237
Alw26I GTCTC 1 cut(s) 255
Alw44I GTGCAC 1 cut(s) 82
AlwI GGATC 1 cut(s) 140
AlwNI CAGNNNCTG 1 cut(s) 334
AoxI GGCC 2 cut(s) 124, 204
ApaLI GTGCAC 1 cut(s) 82
ApeKI GCWGC 4 cut(s) 23, 63, 328, 420
AspLEI GCGC 3 cut(s) 103, 149, 216
AspS9I GGNCC 1 cut(s) 363
AsuC2I CCSGG 2 cut(s) 90, 367
AsuHPI GGTGA 4 cut(s) 61, 184, 334, 389
AvaII GGWCC 1 cut(s) 363
BaeGI GKGCMC 1 cut(s) 86
BalI TGGCCA 1 cut(s) 126
BanI GGYRCC 2 cut(s) 29, 213
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 2 cut(s) 86, 237
BbvI GCAGC 4 cut(s) 35, 50, 340, 407
BccI CCATC 2 cut(s) 40, 215
BceAI ACGGC 1 cut(s) 168
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 1 cut(s) 358
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 2 cut(s) 90, 367
BcoDI GTCTC 1 cut(s) 255
BfaI CTAG 1 cut(s) 454
BfmI CTRYAG 2 cut(s) 21, 432
BfoI RGCGCY 1 cut(s) 217
BglI GCCNNNNNGGC 1 cut(s) 203
BisI GCNGC 6 cut(s) 24, 64, 279, 329, 338, 421
BlsI GCNGC 6 cut(s) 25, 65, 280, 330, 339, 422
Bme1390I CCNGG 3 cut(s) 90, 358, 367
Bme18I GGWCC 1 cut(s) 363
BmgBI CACGTC 2 cut(s) 54, 220
BmgT120I GGNCC 1 cut(s) 363
BmiI GGNNCC 4 cut(s) 6, 31, 215, 376
BmrFI CCNGG 3 cut(s) 90, 358, 367
BmsI GCATC 1 cut(s) 315
BpuMI CCSGG 2 cut(s) 90, 367
BsaHI GRCGYC 2 cut(s) 214, 248
BsaJI CCNNGG 5 cut(s) 9, 121, 254, 276, 356
BsaWI WCCGGW 1 cut(s) 377
Bsc4I CCNNNNNNNGG 1 cut(s) 384
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 5 cut(s) 9, 121, 254, 276, 356
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 384
BseRI GAGGAG 9 cut(s) 29, 89, 92, 271, 299, 302, 305, 308, 311
BseSI GKGCMC 1 cut(s) 86
BseXI GCAGC 4 cut(s) 35, 50, 340, 407
Bsh1236I CGCG 4 cut(s) 103, 149, 278, 384
Bsh1285I CGRYCG 3 cut(s) 159, 245, 382
BshFI GGCC 2 cut(s) 126, 206
BshNI GGYRCC 2 cut(s) 29, 213
BsiEI CGRYCG 3 cut(s) 159, 245, 382
BsiHKAI GWGCWC 2 cut(s) 86, 237
BsiSI CCGG 3 cut(s) 89, 366, 378
BslFI GGGAC 1 cut(s) 382
BslI CCNNNNNNNGG 1 cut(s) 384
BsmAI GTCTC 1 cut(s) 255
BsmBI CGTCTC 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 382
BsnI GGCC 2 cut(s) 126, 206
Bsp1286I GDGCHC 3 cut(s) 9, 86, 237
Bsp143I GATC 5 cut(s) 132, 156, 189, 312, 394
Bsp19I CCATGG 1 cut(s) 121
BspANI GGCC 2 cut(s) 126, 206
BspFNI CGCG 4 cut(s) 103, 149, 278, 384
BspLI GGNNCC 4 cut(s) 6, 31, 215, 376
BspMAI CTGCAG 1 cut(s) 25
BspPI GGATC 1 cut(s) 140
BspQI GCTCTTC 1 cut(s) 410
BspT107I GGYRCC 2 cut(s) 29, 213
BssECI CCNNGG 5 cut(s) 9, 121, 254, 276, 356
BssMI GATC 5 cut(s) 132, 156, 189, 312, 394
BssNI GRCGYC 2 cut(s) 214, 248
BssT1I CCWWGG 1 cut(s) 121
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 2 cut(s) 401, 433
Bst6I CTCTTC 1 cut(s) 410
BstACI GRCGYC 2 cut(s) 214, 248
BstAPI GCANNNNNTGC 2 cut(s) 29, 334
BstC8I GCNNGC 2 cut(s) 86, 140
BstDSI CCRYGG 3 cut(s) 9, 121, 276
BstF5I GGATG 1 cut(s) 177
BstFNI CGCG 4 cut(s) 103, 149, 278, 384
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 3 cut(s) 103, 149, 216
BstKTI GATC 5 cut(s) 135, 159, 192, 315, 397
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 5 cut(s) 132, 156, 189, 312, 394
BstMCI CGRYCG 3 cut(s) 159, 245, 382
BstMWI GCNNNNNNNGC 5 cut(s) 29, 144, 203, 334, 337
BstNI CCWGG 1 cut(s) 358
BstSCI CCNGG 3 cut(s) 88, 356, 365
BstSFI CTRYAG 2 cut(s) 21, 432
BstSLI GKGCMC 1 cut(s) 86
BstUI CGCG 4 cut(s) 103, 149, 278, 384
BstV1I GCAGC 4 cut(s) 35, 50, 340, 407
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
BsuRI GGCC 2 cut(s) 126, 206
BtgI CCRYGG 3 cut(s) 9, 121, 276
BtrI CACGTC 2 cut(s) 54, 220
BtsCI GGATG 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 58
Cac8I GCNNGC 2 cut(s) 86, 140
CaiI CAGNNNCTG 1 cut(s) 334
CfoI GCGC 3 cut(s) 103, 149, 216
Cfr13I GGNCC 1 cut(s) 363
Cfr42I CCGCGG 1 cut(s) 279
CviAII CATG 1 cut(s) 122
CviJI RGCY 7 cut(s) 7, 26, 126, 142, 206, 331, 420
CviKI_1 RGCY 7 cut(s) 7, 26, 126, 142, 206, 331, 420
DinI GGCGCC 1 cut(s) 215
DpnI GATC 5 cut(s) 134, 158, 191, 314, 396
DpnII GATC 5 cut(s) 132, 156, 189, 312, 394
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 410
EarI CTCTTC 1 cut(s) 410
EciI GGCGGA 2 cut(s) 296, 355
Eco130I CCWWGG 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 9
Eco47I GGWCC 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 9
EgeI GGCGCC 1 cut(s) 215
EheI GGCGCC 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 121
Esp3I CGTCTC 1 cut(s) 255
FaeI CATG 1 cut(s) 125
FaiI YATR 3 cut(s) 96, 98, 123
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FaqI GGGAC 1 cut(s) 382
FatI CATG 1 cut(s) 121
FauI CCCGC 4 cut(s) 59, 96, 131, 377
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 1 cut(s) 245
Fnu4HI GCNGC 6 cut(s) 24, 64, 279, 329, 338, 421
FokI GGATG 1 cut(s) 184
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 6 cut(s) 24, 64, 279, 329, 338, 421
FspBI CTAG 1 cut(s) 454
GlaI GCGC 3 cut(s) 102, 148, 215
GluI GCNGC 6 cut(s) 24, 64, 279, 329, 338, 421
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 2 cut(s) 126, 206
HapII CCGG 3 cut(s) 89, 366, 378
HhaI GCGC 3 cut(s) 103, 149, 216
Hin1I GRCGYC 2 cut(s) 214, 248
Hin1II CATG 1 cut(s) 125
Hin6I GCGC 3 cut(s) 101, 147, 214
HinP1I GCGC 3 cut(s) 101, 147, 214
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HinfI GANTC 3 cut(s) 17, 269, 350
HpaII CCGG 3 cut(s) 89, 366, 378
HphI GGTGA 4 cut(s) 61, 184, 334, 389
Hpy166II GTNNAC 2 cut(s) 84, 246
Hpy188I TCNGA 1 cut(s) 255
Hpy188III TCNNGA 1 cut(s) 316
Hpy8I GTNNAC 2 cut(s) 84, 246
Hpy99I CGWCG 3 cut(s) 154, 203, 250
HpyAV CCTTC 2 cut(s) 295, 313
HpyCH4III ACNGT 2 cut(s) 401, 433
HpyCH4IV ACGT 3 cut(s) 53, 219, 248
HpyCH4V TGCA 4 cut(s) 23, 84, 181, 328
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 144, 203, 334, 337
HpySE526I ACGT 3 cut(s) 53, 219, 248
Hsp92I GRCGYC 2 cut(s) 214, 248
Hsp92II CATG 1 cut(s) 125
HspAI GCGC 3 cut(s) 101, 147, 214
KasI GGCGCC 1 cut(s) 213
Ksp22I TGATCA 1 cut(s) 394
KspI CCGCGG 1 cut(s) 279
Kzo9I GATC 5 cut(s) 132, 156, 189, 312, 394
LguI GCTCTTC 1 cut(s) 410
LmnI GCTCC 2 cut(s) 4, 336
LpnPI CCDG 9 cut(s) 12, 102, 196, 343, 347, 370, 379, 391, 433
Lsp1109I GCAGC 4 cut(s) 35, 50, 340, 407
LweI GCATC 1 cut(s) 315
MaeI CTAG 1 cut(s) 454
MaeII ACGT 3 cut(s) 53, 219, 248
MaeIII GTNAC 2 cut(s) 49, 56
MalI GATC 5 cut(s) 134, 158, 191, 314, 396
MboI GATC 5 cut(s) 132, 156, 189, 312, 394
MboII GAAGA 3 cut(s) 199, 264, 427
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 3 cut(s) 9, 86, 237
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 1 cut(s) 442
MluNI TGGCCA 1 cut(s) 126
Mly113I GGCGCC 1 cut(s) 214
MlyI GAGTC 2 cut(s) 26, 344
Mox20I TGGCCA 1 cut(s) 126
MscI TGGCCA 1 cut(s) 126
Msp20I TGGCCA 1 cut(s) 126
MspA1I CMGCKG 2 cut(s) 26, 278
MspI CCGG 3 cut(s) 89, 366, 378
MspR9I CCNGG 3 cut(s) 90, 358, 367
MvaI CCWGG 1 cut(s) 358
MvnI CGCG 4 cut(s) 103, 149, 278, 384
MwoI GCNNNNNNNGC 5 cut(s) 29, 144, 203, 334, 337
NarI GGCGCC 1 cut(s) 214
NciI CCSGG 2 cut(s) 90, 367
NcoI CCATGG 1 cut(s) 121
NdeII GATC 5 cut(s) 132, 156, 189, 312, 394
NlaIII CATG 1 cut(s) 125
NlaIV GGNNCC 4 cut(s) 6, 31, 215, 376
NmuCI GTSAC 2 cut(s) 49, 56
PciSI GCTCTTC 1 cut(s) 410
PcsI WCGNNNNNNNCGW 1 cut(s) 251
PfeI GAWTC 1 cut(s) 269
PkrI GCNGC 6 cut(s) 25, 65, 280, 330, 339, 422
Ple19I CGATCG 1 cut(s) 159
PleI GAGTC 2 cut(s) 25, 344
PluTI GGCGCC 1 cut(s) 217
PpsI GAGTC 2 cut(s) 25, 344
Psp6I CCWGG 1 cut(s) 356
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 4 cut(s) 6, 31, 215, 376
PspPI GGNCC 1 cut(s) 363
PstI CTGCAG 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 334
PsuI RGATCY 1 cut(s) 132
PvuI CGATCG 1 cut(s) 159
PvuII CAGCTG 1 cut(s) 26
SacII CCGCGG 1 cut(s) 279
SalI GTCGAC 1 cut(s) 244
SapI GCTCTTC 1 cut(s) 410
SatI GCNGC 6 cut(s) 24, 64, 279, 329, 338, 421
Sau3AI GATC 5 cut(s) 132, 156, 189, 312, 394
Sau96I GGNCC 1 cut(s) 363
SchI GAGTC 2 cut(s) 26, 344
ScrFI CCNGG 3 cut(s) 90, 358, 367
SduI GDGCHC 3 cut(s) 9, 86, 237
SetI ASST 9 cut(s) 28, 42, 56, 222, 251, 324, 333, 413, 422
SfaNI GCATC 1 cut(s) 315
SfcI CTRYAG 2 cut(s) 21, 432
SfoI GGCGCC 1 cut(s) 215
Sfr303I CCGCGG 1 cut(s) 279
SgrBI CCGCGG 1 cut(s) 279
SinI GGWCC 1 cut(s) 363
Sse9I AATT 1 cut(s) 442
SspDI GGCGCC 1 cut(s) 213
SspMI CTAG 1 cut(s) 454
StyD4I CCNGG 3 cut(s) 88, 356, 365
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 2 cut(s) 401, 433
TaiI ACGT 3 cut(s) 56, 222, 251
TaqI TCGA 4 cut(s) 159, 245, 317, 413
TaqII GACCGA 1 cut(s) 231
TasI AATT 1 cut(s) 442
TauI GCSGC 2 cut(s) 281, 340
TfiI GAWTC 1 cut(s) 269
TscAI CASTG 1 cut(s) 65
TseFI GTSAC 2 cut(s) 49, 56
TseI GCWGC 4 cut(s) 23, 63, 328, 420
Tsp45I GTSAC 2 cut(s) 49, 56
TspDTI ATGAA 1 cut(s) 188
TspRI CASTG 1 cut(s) 65
VneI GTGCAC 1 cut(s) 82
VpaK11BI GGWCC 1 cut(s) 363
XmiI GTMKAC 1 cut(s) 245
XspI CTAG 1 cut(s) 454
ZraI GACGTC 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.