RLG00000020184

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002944
Physical Location & Seq
Reverse (-)
62176109 .. 62176558
450 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020184

Sequence Viewer

Length: 450 bp
ATGGGAGGTCTATCAGCCCCAGAAAGCCAAGCAGCAGCCCCAAAACTCAATCCCCAATCAGAGATTGAGTCTGCAAAGTGTGATTGCTGTGGGCTCACAGAGGAGTGCACCCCCGCCTATATAGAACATGTCCGAGAGAGAAACATGGGCCATTGGATTTGTGGGTTGTGTTCTGAGGCTATAAAAGATGAGATTGTGAGGTCTGAGAGGCTAGTTAGCACTGAAGAAGCCATGGCTACACATATGAGCTTTTGCAAGAAATTTAAGGCATCCGGGCCTCCTTTGAACCCTGCTATTCATTTGATATCTGCCATGAGACAGATTCTCCGGCGTAGTTTGGACTCTCCGAGGGGCTCGAATTCAACTCCGAATAGTCCGATGAGGATGGGTGGCCGCGCGGCGCTTACTAGGTCCGGGAGTTGCTTTCCTGCCCTGACCGGAGTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

150

Amino Acids

16.06

Weight (kDa)

7.58

Isoelectric Point (pI)

80.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 22 - 115 5.1e-41 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 396, 398
AciI CCGC 3 cut(s) 114, 394, 398
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 2 cut(s) 260, 358
AcuI CTGAAG 1 cut(s) 243
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 2 cut(s) 286, 363
AluBI AGCT 1 cut(s) 249
AluI AGCT 1 cut(s) 249
Alw21I GWGCWC 1 cut(s) 110
Alw26I GTCTC 1 cut(s) 310
Alw44I GTGCAC 1 cut(s) 106
AoxI GGCC 3 cut(s) 148, 275, 391
ApaLI GTGCAC 1 cut(s) 106
ApeKI GCWGC 2 cut(s) 32, 35
ApoI RAATTY 2 cut(s) 260, 358
AspLEI GCGC 2 cut(s) 398, 403
AspS9I GGNCC 3 cut(s) 148, 275, 411
AsuC2I CCSGG 2 cut(s) 274, 415
AvaII GGWCC 1 cut(s) 411
BaeGI GKGCMC 1 cut(s) 110
BanII GRGCYC 2 cut(s) 96, 356
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 2 cut(s) 44, 47
BccI CCATC 1 cut(s) 379
BcnI CCSGG 2 cut(s) 274, 415
BcoDI GTCTC 1 cut(s) 310
BfaI CTAG 2 cut(s) 212, 408
BfoI RGCGCY 1 cut(s) 404
BisI GCNGC 4 cut(s) 33, 36, 394, 399
BlsI GCNGC 4 cut(s) 34, 37, 395, 400
Bme1390I CCNGG 2 cut(s) 274, 415
Bme18I GGWCC 1 cut(s) 411
BmgT120I GGNCC 3 cut(s) 148, 275, 411
BmrFI CCNGG 2 cut(s) 274, 415
BmsI GCATC 1 cut(s) 278
BpuMI CCSGG 2 cut(s) 274, 415
BsaJI CCNNGG 2 cut(s) 231, 347
BsaWI WCCGGW 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
BseDI CCNNGG 2 cut(s) 231, 347
BseGI GGATG 2 cut(s) 269, 390
BseMII CTCAG 2 cut(s) 165, 195
BseRI GAGGAG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 110
BseXI GCAGC 2 cut(s) 44, 47
Bsh1236I CGCG 2 cut(s) 396, 398
BshFI GGCC 3 cut(s) 150, 277, 393
BsiHKAI GWGCWC 1 cut(s) 110
BsiSI CCGG 4 cut(s) 273, 328, 414, 438
BsmAI GTCTC 1 cut(s) 310
BsnI GGCC 3 cut(s) 150, 277, 393
Bsp1286I GDGCHC 3 cut(s) 96, 110, 356
Bsp19I CCATGG 1 cut(s) 231
BspACI CCGC 3 cut(s) 114, 394, 398
BspANI GGCC 3 cut(s) 150, 277, 393
BspCNI CTCAG 2 cut(s) 166, 196
BspFNI CGCG 2 cut(s) 396, 398
BssECI CCNNGG 2 cut(s) 231, 347
BssT1I CCWWGG 1 cut(s) 231
BstDEI CTNAG 2 cut(s) 174, 204
BstDSI CCRYGG 1 cut(s) 231
BstF5I GGATG 2 cut(s) 269, 390
BstFNI CGCG 2 cut(s) 396, 398
BstH2I RGCGCY 1 cut(s) 404
BstHHI GCGC 2 cut(s) 398, 403
BstMAI GTCTC 1 cut(s) 310
BstNSI RCATGY 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 272, 413
BstSLI GKGCMC 1 cut(s) 110
BstUI CGCG 2 cut(s) 396, 398
BstV1I GCAGC 2 cut(s) 44, 47
BsuRI GGCC 3 cut(s) 150, 277, 393
BtgI CCRYGG 1 cut(s) 231
BtsCI GGATG 2 cut(s) 269, 390
BtsIMutI CAGTG 1 cut(s) 219
CfoI GCGC 2 cut(s) 398, 403
Cfr13I GGNCC 3 cut(s) 148, 275, 411
CviAII CATG 4 cut(s) 128, 145, 232, 313
DdeI CTNAG 2 cut(s) 174, 204
EaeI YGGCCR 1 cut(s) 391
Eco130I CCWWGG 1 cut(s) 231
Eco24I GRGCYC 2 cut(s) 96, 356
Eco32I GATATC 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 411
Eco57I CTGAAG 1 cut(s) 243
EcoRI GAATTC 1 cut(s) 358
EcoRV GATATC 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 231
EcoT38I GRGCYC 2 cut(s) 96, 356
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 4 cut(s) 131, 148, 235, 316
FaiI YATR 9 cut(s) 120, 122, 129, 146, 182, 233, 243, 245, 314
FatI CATG 4 cut(s) 127, 144, 231, 312
FauI CCCGC 1 cut(s) 121
FauNDI CATATG 1 cut(s) 243
Fnu4HI GCNGC 4 cut(s) 33, 36, 394, 399
FokI GGATG 2 cut(s) 256, 397
FriOI GRGCYC 2 cut(s) 96, 356
Fsp4HI GCNGC 4 cut(s) 33, 36, 394, 399
FspBI CTAG 2 cut(s) 212, 408
GlaI GCGC 2 cut(s) 397, 402
GluI GCNGC 4 cut(s) 33, 36, 394, 399
HaeII RGCGCY 1 cut(s) 404
HaeIII GGCC 3 cut(s) 150, 277, 393
HapII CCGG 4 cut(s) 273, 328, 414, 438
HhaI GCGC 2 cut(s) 398, 403
Hin1II CATG 4 cut(s) 131, 148, 235, 316
Hin6I GCGC 2 cut(s) 396, 401
HinP1I GCGC 2 cut(s) 396, 401
HinfI GANTC 3 cut(s) 68, 322, 341
HpaII CCGG 4 cut(s) 273, 328, 414, 438
Hpy166II GTNNAC 1 cut(s) 108
Hpy188I TCNGA 7 cut(s) 61, 134, 175, 205, 348, 369, 378
Hpy8I GTNNAC 1 cut(s) 108
HpyCH4V TGCA 3 cut(s) 74, 108, 255
HpyF3I CTNAG 2 cut(s) 174, 204
Hsp92II CATG 4 cut(s) 131, 148, 235, 316
HspAI GCGC 2 cut(s) 396, 401
LpnPI CCDG 7 cut(s) 33, 286, 303, 341, 427, 441, 446
Lsp1109I GCAGC 2 cut(s) 44, 47
LweI GCATC 1 cut(s) 278
MaeI CTAG 2 cut(s) 212, 408
MboII GAAGA 1 cut(s) 236
MhlI GDGCHC 3 cut(s) 96, 110, 356
MluCI AATT 2 cut(s) 260, 358
MlyI GAGTC 2 cut(s) 77, 335
MnlI CCTC 7 cut(s) 94, 169, 192, 201, 288, 342, 375
MseI TTAA 1 cut(s) 264
MspI CCGG 4 cut(s) 273, 328, 414, 438
MspR9I CCNGG 2 cut(s) 274, 415
MteI GCGCNGCGC 1 cut(s) 399
MvnI CGCG 2 cut(s) 396, 398
NciI CCSGG 2 cut(s) 274, 415
NcoI CCATGG 1 cut(s) 231
NdeI CATATG 1 cut(s) 243
NlaIII CATG 4 cut(s) 131, 148, 235, 316
NspI RCATGY 1 cut(s) 131
PciI ACATGT 1 cut(s) 127
PfeI GAWTC 1 cut(s) 322
PfoI TCCNGGA 1 cut(s) 413
PkrI GCNGC 4 cut(s) 34, 37, 395, 400
PleI GAGTC 2 cut(s) 76, 335
PpsI GAGTC 2 cut(s) 76, 335
PscI ACATGT 1 cut(s) 127
PspPI GGNCC 3 cut(s) 148, 275, 411
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 4 cut(s) 33, 36, 394, 399
Sau96I GGNCC 3 cut(s) 148, 275, 411
SchI GAGTC 2 cut(s) 77, 335
ScrFI CCNGG 2 cut(s) 274, 415
SduI GDGCHC 3 cut(s) 96, 110, 356
SetI ASST 4 cut(s) 10, 203, 251, 413
SfaNI GCATC 1 cut(s) 278
SinI GGWCC 1 cut(s) 411
Sse9I AATT 2 cut(s) 260, 358
SsiI CCGC 3 cut(s) 114, 394, 398
SspMI CTAG 2 cut(s) 212, 408
StyD4I CCNGG 2 cut(s) 272, 413
StyI CCWWGG 1 cut(s) 231
TaqI TCGA 1 cut(s) 356
TasI AATT 2 cut(s) 260, 358
TauI GCSGC 2 cut(s) 396, 401
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 1 cut(s) 226
TseI GCWGC 2 cut(s) 32, 35
TspDTI ATGAA 1 cut(s) 287
TspRI CASTG 1 cut(s) 226
VneI GTGCAC 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 411
XapI RAATTY 2 cut(s) 260, 358
XceI RCATGY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XspI CTAG 2 cut(s) 212, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.