pycom09g09780

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002944
Physical Location & Seq
Forward (+)
7918628 .. 7918942
315 bp
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UTR
Exon/CDS
Intron
pycom09g09780.1

Sequence Viewer

Length: 315 bp
ATGGCAATGTCCGCAACTATCGTCACCACCGACACGATTGTTCTACCGGCCGCGGCGGCGGAAACTCAGCCACAACAAACCACAACAAAACTCGTTCCCTCCATCGAAATCGAGTTCGTACAATGCGACTGCTGTGGATTAACAGAAGAGTGCACACCTGCGTACATTGAGCACGTGAGGGAGAGATATCAGGGCCATTGGATTTGTGGGTTGTGTGCAGAAGCTATAAAAGATGAGATTGTTAGGTCGCAGAAGAGGCTGATCGAGCACGGAAGAAGCCATGGCCACACACATGAACTTCTGCAAAAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

105

Amino Acids

11.62

Weight (kDa)

5.6

Isoelectric Point (pI)

67.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 37 - 86 1e-24 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 166
Acc36I ACCTGC 1 cut(s) 166
AccII CGCG 1 cut(s) 53
AciI CCGC 5 cut(s) 12, 51, 53, 56, 59
AcoI YGGCCR 2 cut(s) 48, 283
AcvI CACGTG 1 cut(s) 175
AfaI GTAC 2 cut(s) 120, 164
AluBI AGCT 1 cut(s) 224
AluI AGCT 1 cut(s) 224
Alw21I GWGCWC 3 cut(s) 155, 174, 270
Alw44I GTGCAC 1 cut(s) 151
AoxI GGCC 3 cut(s) 48, 193, 283
ApaLI GTGCAC 1 cut(s) 151
AspS9I GGNCC 1 cut(s) 193
AsuHPI GGTGA 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 155
BalI TGGCCA 1 cut(s) 285
BbrPI CACGTG 1 cut(s) 175
Bbv12I GWGCWC 3 cut(s) 155, 174, 270
BccI CCATC 1 cut(s) 110
BfuAI ACCTGC 1 cut(s) 166
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 3 cut(s) 51, 54, 57
BlsI GCNGC 3 cut(s) 52, 55, 58
BmgT120I GGNCC 1 cut(s) 193
BsaAI YACGTR 1 cut(s) 175
BsaJI CCNNGG 2 cut(s) 51, 280
Bse118I RCCGGY 1 cut(s) 46
Bse3DI GCAATG 1 cut(s) 12
BseDI CCNNGG 2 cut(s) 51, 280
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 155
BseX3I CGGCCG 1 cut(s) 48
BsgI GTGCAG 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 53
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 3 cut(s) 50, 195, 285
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 3 cut(s) 155, 174, 270
BsiSI CCGG 1 cut(s) 47
BsnI GGCC 3 cut(s) 50, 195, 285
Bsp1286I GDGCHC 3 cut(s) 155, 174, 270
Bsp143I GATC 1 cut(s) 261
Bsp19I CCATGG 1 cut(s) 280
BspACI CCGC 5 cut(s) 12, 51, 53, 56, 59
BspANI GGCC 3 cut(s) 50, 195, 285
BspCNI CTCAG 1 cut(s) 79
BspFNI CGCG 1 cut(s) 53
BspMI ACCTGC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 46
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 51, 280
BssMI GATC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 280
Bst6I CTCTTC 2 cut(s) 141, 248
BstBAI YACGTR 1 cut(s) 175
BstDEI CTNAG 1 cut(s) 66
BstDSI CCRYGG 2 cut(s) 51, 280
BstFNI CGCG 1 cut(s) 53
BstKTI GATC 1 cut(s) 264
BstMBI GATC 1 cut(s) 261
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 4 cut(s) 11, 56, 256, 265
BstSLI GKGCMC 1 cut(s) 155
BstUI CGCG 1 cut(s) 53
BstZI CGGCCG 1 cut(s) 48
BsuRI GGCC 3 cut(s) 50, 195, 285
BtgI CCRYGG 2 cut(s) 51, 280
BveI ACCTGC 1 cut(s) 166
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 193
Cfr42I CCGCGG 1 cut(s) 54
Csp6I GTAC 2 cut(s) 119, 163
CviAII CATG 2 cut(s) 281, 293
CviJI RGCY 7 cut(s) 50, 70, 195, 224, 259, 279, 285
CviKI_1 RGCY 7 cut(s) 50, 70, 195, 224, 259, 279, 285
CviQI GTAC 2 cut(s) 119, 163
DdeI CTNAG 1 cut(s) 66
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
EaeI YGGCCR 2 cut(s) 48, 283
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 2 cut(s) 141, 248
EarI CTCTTC 2 cut(s) 141, 248
EciI GGCGGA 1 cut(s) 74
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 280
Eco32I GATATC 1 cut(s) 188
Eco52I CGGCCG 1 cut(s) 48
Eco72I CACGTG 1 cut(s) 175
EcoRV GATATC 1 cut(s) 188
EcoT14I CCWWGG 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 280
FaeI CATG 2 cut(s) 284, 296
FaiI YATR 3 cut(s) 227, 282, 294
FatI CATG 2 cut(s) 280, 292
Fnu4HI GCNGC 3 cut(s) 51, 54, 57
Fsp4HI GCNGC 3 cut(s) 51, 54, 57
GluI GCNGC 3 cut(s) 51, 54, 57
HaeIII GGCC 3 cut(s) 50, 195, 285
HapII CCGG 1 cut(s) 47
Hin1II CATG 2 cut(s) 284, 296
HpaII CCGG 1 cut(s) 47
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 153
Hpy8I GTNNAC 1 cut(s) 153
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 3 cut(s) 153, 218, 304
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 56, 256, 265
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 2 cut(s) 284, 296
KspI CCGCGG 1 cut(s) 54
Kzo9I GATC 1 cut(s) 261
LpnPI CCDG 3 cut(s) 60, 171, 176
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 1 cut(s) 22
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 3 cut(s) 158, 265, 285
MhlI GDGCHC 3 cut(s) 155, 174, 270
MlsI TGGCCA 1 cut(s) 285
MluNI TGGCCA 1 cut(s) 285
MnlI CCTC 3 cut(s) 109, 171, 249
Mox20I TGGCCA 1 cut(s) 285
MscI TGGCCA 1 cut(s) 285
MseI TTAA 2 cut(s) 140, 313
MslI CAYNNNNRTG 1 cut(s) 291
Msp20I TGGCCA 1 cut(s) 285
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 1 cut(s) 47
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 4 cut(s) 11, 56, 256, 265
NcoI CCATGG 1 cut(s) 280
NdeII GATC 1 cut(s) 261
NlaIII CATG 2 cut(s) 284, 296
NmuCI GTSAC 1 cut(s) 22
PaqCI CACCTGC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 2 cut(s) 27, 123
PkrI GCNGC 3 cut(s) 52, 55, 58
PmaCI CACGTG 1 cut(s) 175
PmlI CACGTG 1 cut(s) 175
Ppu21I YACGTR 1 cut(s) 175
PspCI CACGTG 1 cut(s) 175
PspPI GGNCC 1 cut(s) 193
RsaI GTAC 2 cut(s) 120, 164
RsaNI GTAC 2 cut(s) 119, 163
RseI CAYNNNNRTG 1 cut(s) 291
SacII CCGCGG 1 cut(s) 54
SaqAI TTAA 2 cut(s) 140, 313
SatI GCNGC 3 cut(s) 51, 54, 57
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 1 cut(s) 193
SduI GDGCHC 3 cut(s) 155, 174, 270
SetI ASST 4 cut(s) 160, 177, 226, 248
Sfr303I CCGCGG 1 cut(s) 54
SgrBI CCGCGG 1 cut(s) 54
SmiMI CAYNNNNRTG 1 cut(s) 291
SsiI CCGC 5 cut(s) 12, 51, 53, 56, 59
StyI CCWWGG 1 cut(s) 280
TaiI ACGT 1 cut(s) 177
TaqI TCGA 3 cut(s) 105, 111, 264
TauI GCSGC 3 cut(s) 53, 56, 59
Tru1I TTAA 2 cut(s) 140, 313
Tru9I TTAA 2 cut(s) 140, 313
TseFI GTSAC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 1 cut(s) 309
TspGWI ACGGA 1 cut(s) 285
VneI GTGCAC 1 cut(s) 151
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.