Rroxscaffold_7G00157100

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002944
Physical Location & Seq
Forward (+)
919511 .. 919975
465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00157100.1

Sequence Viewer

Length: 465 bp
ATGGCATTAGTCTCTCCGATGGAGGAGTCTGCTGCTGGTGCCGATGAGGTGGAGATGGTGACGTGTCACTGCTGCGGGTTAGAGGAGGAGTGCACGCCGGGATATATAGCGCGGGAAAGGGAGAAGCACCATGGCCGTTGGATCTGCGGGCTTTGCTCGACGGCGATCGAGGACGACAGGATGAAGTTGCAGAGGACGATCACCGCCGACGAGGCCATCAGGCGCCACGTCAAGTTTTACGAGAACTTTAGGTCGACGTCTCCGAGGAGGAGTGTGGATTCTTCCGCGGCGGAGGAGGAGGAGGAGGAGAAGGACTTGTTGATCTCGAAGGTGATGCAGCTCCTGCGGCGGACTTTGGACTCGCCGAGGAGGACCGGGACGGGTTCCGGTCGCGGGGCGTTTGATCACCGTCTTTTGAGGTCGAAGAGCTGCGTTGCGACTCTAGGAGAGAAGGGTCTAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

154

Amino Acids

17.3

Weight (kDa)

5.79

Isoelectric Point (pI)

76.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 17 - 92 4.2e-26 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 260
AccB1I GGYRCC 2 cut(s) 38, 222
AccI GTMKAC 1 cut(s) 254
AccII CGCG 3 cut(s) 112, 287, 393
AclWI GGATC 1 cut(s) 149
AcoI YGGCCR 1 cut(s) 133
AcyI GRCGYC 2 cut(s) 223, 257
AfiI CCNNNNNNNGG 1 cut(s) 393
AflIII ACRYGT 1 cut(s) 62
AjiI CACGTC 2 cut(s) 63, 229
AluBI AGCT 2 cut(s) 340, 429
AluI AGCT 2 cut(s) 340, 429
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 2 cut(s) 16, 264
Alw44I GTGCAC 1 cut(s) 91
AlwI GGATC 1 cut(s) 149
AlwNI CAGNNNCTG 1 cut(s) 343
AoxI GGCC 2 cut(s) 133, 213
ApaLI GTGCAC 1 cut(s) 91
ApeKI GCWGC 4 cut(s) 32, 72, 337, 429
AspLEI GCGC 2 cut(s) 112, 225
AspS9I GGNCC 1 cut(s) 372
AsuC2I CCSGG 2 cut(s) 99, 376
AsuHPI GGTGA 4 cut(s) 70, 193, 343, 398
AvaII GGWCC 1 cut(s) 372
BaeGI GKGCMC 1 cut(s) 95
BanI GGYRCC 2 cut(s) 38, 222
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 4 cut(s) 19, 59, 349, 416
BccI CCATC 3 cut(s) 13, 49, 224
BceAI ACGGC 2 cut(s) 120, 177
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 2 cut(s) 99, 376
BcoDI GTCTC 2 cut(s) 16, 264
BfaI CTAG 2 cut(s) 443, 458
BfoI RGCGCY 1 cut(s) 226
BglI GCCNNNNNGGC 1 cut(s) 212
BisI GCNGC 6 cut(s) 33, 73, 288, 338, 347, 430
BlsI GCNGC 6 cut(s) 34, 74, 289, 339, 348, 431
Bme1390I CCNGG 2 cut(s) 99, 376
Bme18I GGWCC 1 cut(s) 372
BmgBI CACGTC 2 cut(s) 63, 229
BmgT120I GGNCC 1 cut(s) 372
BmiI GGNNCC 3 cut(s) 40, 224, 385
BmrFI CCNGG 2 cut(s) 99, 376
BmsI GCATC 1 cut(s) 324
BpuMI CCSGG 2 cut(s) 99, 376
BsaHI GRCGYC 2 cut(s) 223, 257
BsaJI CCNNGG 4 cut(s) 130, 263, 285, 365
BsaWI WCCGGW 1 cut(s) 386
Bsc4I CCNNNNNNNGG 1 cut(s) 393
BseDI CCNNGG 4 cut(s) 130, 263, 285, 365
BseGI GGATG 1 cut(s) 186
BseLI CCNNNNNNNGG 1 cut(s) 393
BseSI GKGCMC 1 cut(s) 95
BseXI GCAGC 4 cut(s) 19, 59, 349, 416
Bsh1236I CGCG 3 cut(s) 112, 287, 393
Bsh1285I CGRYCG 2 cut(s) 168, 391
BshFI GGCC 2 cut(s) 135, 215
BshNI GGYRCC 2 cut(s) 38, 222
BsiEI CGRYCG 2 cut(s) 168, 391
BsiHKAI GWGCWC 1 cut(s) 95
BsiSI CCGG 3 cut(s) 98, 375, 387
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 1 cut(s) 393
BsmAI GTCTC 2 cut(s) 16, 264
BsmBI CGTCTC 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 391
BsnI GGCC 2 cut(s) 135, 215
Bsp1286I GDGCHC 1 cut(s) 95
Bsp143I GATC 5 cut(s) 141, 165, 198, 321, 403
Bsp19I CCATGG 1 cut(s) 130
BspANI GGCC 2 cut(s) 135, 215
BspFNI CGCG 3 cut(s) 112, 287, 393
BspLI GGNNCC 3 cut(s) 40, 224, 385
BspPI GGATC 1 cut(s) 149
BspQI GCTCTTC 1 cut(s) 419
BspT107I GGYRCC 2 cut(s) 38, 222
BssECI CCNNGG 4 cut(s) 130, 263, 285, 365
BssMI GATC 5 cut(s) 141, 165, 198, 321, 403
BssNI GRCGYC 2 cut(s) 223, 257
BssT1I CCWWGG 1 cut(s) 130
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 1 cut(s) 419
BstACI GRCGYC 2 cut(s) 223, 257
BstAPI GCANNNNNTGC 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 95, 149
BstDSI CCRYGG 2 cut(s) 130, 285
BstF5I GGATG 1 cut(s) 186
BstFNI CGCG 3 cut(s) 112, 287, 393
BstH2I RGCGCY 1 cut(s) 226
BstHHI GCGC 2 cut(s) 112, 225
BstKTI GATC 5 cut(s) 144, 168, 201, 324, 406
BstMAI GTCTC 2 cut(s) 16, 264
BstMBI GATC 5 cut(s) 141, 165, 198, 321, 403
BstMCI CGRYCG 2 cut(s) 168, 391
BstMWI GCNNNNNNNGC 5 cut(s) 38, 153, 212, 343, 346
BstSCI CCNGG 2 cut(s) 97, 374
BstSLI GKGCMC 1 cut(s) 95
BstUI CGCG 3 cut(s) 112, 287, 393
BstV1I GCAGC 4 cut(s) 19, 59, 349, 416
BstX2I RGATCY 1 cut(s) 141
BstYI RGATCY 1 cut(s) 141
BsuRI GGCC 2 cut(s) 135, 215
BtgI CCRYGG 2 cut(s) 130, 285
BtrI CACGTC 2 cut(s) 63, 229
BtsCI GGATG 1 cut(s) 186
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 1 cut(s) 67
Cac8I GCNNGC 2 cut(s) 95, 149
CaiI CAGNNNCTG 1 cut(s) 343
CfoI GCGC 2 cut(s) 112, 225
Cfr13I GGNCC 1 cut(s) 372
Cfr42I CCGCGG 1 cut(s) 288
CviAII CATG 1 cut(s) 131
CviJI RGCY 5 cut(s) 135, 151, 215, 340, 429
CviKI_1 RGCY 5 cut(s) 135, 151, 215, 340, 429
DinI GGCGCC 1 cut(s) 224
DpnI GATC 5 cut(s) 143, 167, 200, 323, 405
DpnII GATC 5 cut(s) 141, 165, 198, 321, 403
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 419
EarI CTCTTC 1 cut(s) 419
EciI GGCGGA 2 cut(s) 305, 364
Eco130I CCWWGG 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 130
EgeI GGCGCC 1 cut(s) 224
EheI GGCGCC 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 130
Esp3I CGTCTC 1 cut(s) 264
FaeI CATG 1 cut(s) 134
FaiI YATR 3 cut(s) 105, 107, 132
FaqI GGGAC 1 cut(s) 391
FatI CATG 1 cut(s) 130
FauI CCCGC 4 cut(s) 68, 105, 140, 386
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 1 cut(s) 254
Fnu4HI GCNGC 6 cut(s) 33, 73, 288, 338, 347, 430
FokI GGATG 1 cut(s) 193
Fsp4HI GCNGC 6 cut(s) 33, 73, 288, 338, 347, 430
FspBI CTAG 2 cut(s) 443, 458
GlaI GCGC 2 cut(s) 111, 224
GluI GCNGC 6 cut(s) 33, 73, 288, 338, 347, 430
HaeII RGCGCY 1 cut(s) 226
HaeIII GGCC 2 cut(s) 135, 215
HapII CCGG 3 cut(s) 98, 375, 387
HhaI GCGC 2 cut(s) 112, 225
Hin1I GRCGYC 2 cut(s) 223, 257
Hin1II CATG 1 cut(s) 134
Hin6I GCGC 2 cut(s) 110, 223
HinP1I GCGC 2 cut(s) 110, 223
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HinfI GANTC 4 cut(s) 26, 278, 359, 439
HpaII CCGG 3 cut(s) 98, 375, 387
HphI GGTGA 4 cut(s) 70, 193, 343, 398
Hpy166II GTNNAC 2 cut(s) 93, 255
Hpy188I TCNGA 2 cut(s) 18, 264
Hpy188III TCNNGA 2 cut(s) 325, 458
Hpy8I GTNNAC 2 cut(s) 93, 255
Hpy99I CGWCG 3 cut(s) 163, 212, 259
HpyAV CCTTC 3 cut(s) 304, 322, 445
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4IV ACGT 3 cut(s) 62, 228, 257
HpyCH4V TGCA 3 cut(s) 93, 190, 337
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 153, 212, 343, 346
HpySE526I ACGT 3 cut(s) 62, 228, 257
Hsp92I GRCGYC 2 cut(s) 223, 257
Hsp92II CATG 1 cut(s) 134
HspAI GCGC 2 cut(s) 110, 223
KasI GGCGCC 1 cut(s) 222
Ksp22I TGATCA 1 cut(s) 403
KspI CCGCGG 1 cut(s) 288
Kzo9I GATC 5 cut(s) 141, 165, 198, 321, 403
LguI GCTCTTC 1 cut(s) 419
LmnI GCTCC 1 cut(s) 345
LpnPI CCDG 7 cut(s) 21, 111, 163, 205, 356, 388, 400
Lsp1109I GCAGC 4 cut(s) 19, 59, 349, 416
LweI GCATC 1 cut(s) 324
MaeI CTAG 2 cut(s) 443, 458
MaeII ACGT 3 cut(s) 62, 228, 257
MaeIII GTNAC 2 cut(s) 58, 65
MalI GATC 5 cut(s) 143, 167, 200, 323, 405
MboI GATC 5 cut(s) 141, 165, 198, 321, 403
MboII GAAGA 2 cut(s) 273, 436
MflI RGATCY 1 cut(s) 141
MhlI GDGCHC 1 cut(s) 95
Mly113I GGCGCC 1 cut(s) 223
MlyI GAGTC 3 cut(s) 35, 353, 433
MmeI TCCRAC 1 cut(s) 119
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 3 cut(s) 98, 375, 387
MspR9I CCNGG 2 cut(s) 99, 376
MvnI CGCG 3 cut(s) 112, 287, 393
MwoI GCNNNNNNNGC 5 cut(s) 38, 153, 212, 343, 346
NarI GGCGCC 1 cut(s) 223
NciI CCSGG 2 cut(s) 99, 376
NcoI CCATGG 1 cut(s) 130
NdeII GATC 5 cut(s) 141, 165, 198, 321, 403
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 3 cut(s) 40, 224, 385
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 2 cut(s) 58, 65
PciSI GCTCTTC 1 cut(s) 419
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PfeI GAWTC 1 cut(s) 278
PkrI GCNGC 6 cut(s) 34, 74, 289, 339, 348, 431
Ple19I CGATCG 1 cut(s) 168
PleI GAGTC 3 cut(s) 34, 353, 433
PluTI GGCGCC 1 cut(s) 226
PpsI GAGTC 3 cut(s) 34, 353, 433
PspN4I GGNNCC 3 cut(s) 40, 224, 385
PspPI GGNCC 1 cut(s) 372
PstNI CAGNNNCTG 1 cut(s) 343
PsuI RGATCY 1 cut(s) 141
PvuI CGATCG 1 cut(s) 168
SacII CCGCGG 1 cut(s) 288
SalI GTCGAC 1 cut(s) 253
SapI GCTCTTC 1 cut(s) 419
SatI GCNGC 6 cut(s) 33, 73, 288, 338, 347, 430
Sau3AI GATC 5 cut(s) 141, 165, 198, 321, 403
Sau96I GGNCC 1 cut(s) 372
SchI GAGTC 3 cut(s) 35, 353, 433
ScrFI CCNGG 2 cut(s) 99, 376
SduI GDGCHC 1 cut(s) 95
SetI ASST 9 cut(s) 51, 65, 231, 254, 260, 333, 342, 422, 431
SfaNI GCATC 1 cut(s) 324
SfoI GGCGCC 1 cut(s) 224
Sfr303I CCGCGG 1 cut(s) 288
SgrBI CCGCGG 1 cut(s) 288
SinI GGWCC 1 cut(s) 372
SspDI GGCGCC 1 cut(s) 222
SspMI CTAG 2 cut(s) 443, 458
StyD4I CCNGG 2 cut(s) 97, 374
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 1 cut(s) 410
TaiI ACGT 3 cut(s) 65, 231, 260
TaqI TCGA 5 cut(s) 158, 168, 254, 326, 422
TauI GCSGC 2 cut(s) 290, 349
TfiI GAWTC 1 cut(s) 278
TscAI CASTG 1 cut(s) 74
TseFI GTSAC 2 cut(s) 58, 65
TseI GCWGC 4 cut(s) 32, 72, 337, 429
Tsp45I GTSAC 2 cut(s) 58, 65
TspDTI ATGAA 1 cut(s) 197
TspRI CASTG 1 cut(s) 74
VneI GTGCAC 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 372
XbaI TCTAGA 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 254
XspI CTAG 2 cut(s) 443, 458
ZraI GACGTC 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.