MD08G1046000.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002944
Physical Location & Seq
Reverse (-)
3478425 .. 3478829
405 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1046000.v1.1.491

Sequence Viewer

Length: 405 bp
ATGGAGTCGGCGGAGGTGAAGCGGATTTCGGAGCCGGAGGAGGTGGTGGAGGTGAAGTGCTACTGCTGCTGTTTGAAGGAGGAGTGCACACCGGCGTACGTAGCGGCGGTGAAGGAGAGGCACCAGGGCCGGTGGATTTGCGGACTCTGCACTGAGGCGATCAAGGACGAGAACTCGAGGTCGCCGAGGAAGATTAGCACGGAGGAGGCGGTGAAGCGGCACTTCAGATTCTGCAAGGAGTTTCGGAAGTCGATTCCTCCCGCGAACCCGACGGCGGATCTGATATCGGCAATGAAACAGCTGTTGCTGCGGACTCTGGACTCGCCGAGGTTGGGCGACTGCCGGCCGGCGATGGTGAGGTCGAAGAGCTGCTTTGCGACTGTGGGGAAGGCACAGGGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

135

Amino Acids

15.09

Weight (kDa)

8.84

Isoelectric Point (pI)

68.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 16 - 108 1.5e-36 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 120
AccII CGCG 1 cut(s) 263
AclWI GGATC 1 cut(s) 285
AcoI YGGCCR 1 cut(s) 344
AcuI CTGAAG 1 cut(s) 208
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 274, 332
AgsI TTSAA 1 cut(s) 76
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 2 cut(s) 301, 369
AluI AGCT 2 cut(s) 301, 369
Alw21I GWGCWC 1 cut(s) 89
Alw44I GTGCAC 1 cut(s) 85
AlwI GGATC 1 cut(s) 285
AlwNI CAGNNNCTG 1 cut(s) 231
Ama87I CYCGRG 1 cut(s) 175
AoxI GGCC 2 cut(s) 127, 344
ApaLI GTGCAC 1 cut(s) 85
ApeKI GCWGC 3 cut(s) 66, 307, 369
AspS9I GGNCC 1 cut(s) 127
AsuHPI GGTGA 5 cut(s) 28, 64, 121, 223, 367
AvaI CYCGRG 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 89
BanI GGYRCC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 89
BbvI GCAGC 3 cut(s) 53, 294, 356
BccI CCATC 1 cut(s) 346
BceAI ACGGC 1 cut(s) 288
BciT130I CCWGG 1 cut(s) 125
BisI GCNGC 5 cut(s) 67, 105, 218, 308, 370
BlsI GCNGC 5 cut(s) 68, 106, 219, 309, 371
Bme1390I CCNGG 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 2 cut(s) 33, 122
BmrFI CCNGG 1 cut(s) 125
BsaAI YACGTR 1 cut(s) 100
BsaJI CCNNGG 3 cut(s) 124, 185, 326
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 2 cut(s) 274, 332
Bse118I RCCGGY 4 cut(s) 91, 129, 342, 346
Bse3DI GCAATG 1 cut(s) 297
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 3 cut(s) 124, 185, 326
BseLI CCNNNNNNNGG 2 cut(s) 274, 332
BseMI GCAATG 1 cut(s) 297
BseMII CTCAG 1 cut(s) 144
BseRI GAGGAG 3 cut(s) 53, 95, 218
BseSI GKGCMC 1 cut(s) 89
BseX3I CGGCCG 1 cut(s) 344
BseXI GCAGC 3 cut(s) 53, 294, 356
BsgI GTGCAG 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 263
Bsh1285I CGRYCG 1 cut(s) 347
BshFI GGCC 2 cut(s) 129, 346
BshNI GGYRCC 1 cut(s) 120
BsiEI CGRYCG 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 89
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 5 cut(s) 35, 92, 130, 343, 347
BsiWI CGTACG 1 cut(s) 96
BslI CCNNNNNNNGG 2 cut(s) 274, 332
BsnI GGCC 2 cut(s) 129, 346
BsoBI CYCGRG 1 cut(s) 175
Bsp1286I GDGCHC 1 cut(s) 89
Bsp143I GATC 2 cut(s) 159, 277
BspANI GGCC 2 cut(s) 129, 346
BspCNI CTCAG 1 cut(s) 145
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 2 cut(s) 33, 122
BspPI GGATC 1 cut(s) 285
BspQI GCTCTTC 1 cut(s) 359
BspT107I GGYRCC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 297
BsrFI RCCGGY 4 cut(s) 91, 129, 342, 346
BssAI RCCGGY 4 cut(s) 91, 129, 342, 346
BssECI CCNNGG 3 cut(s) 124, 185, 326
BssMI GATC 2 cut(s) 159, 277
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 1 cut(s) 382
Bst6I CTCTTC 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 100
BstC8I GCNNGC 2 cut(s) 344, 348
BstDEI CTNAG 1 cut(s) 153
BstFNI CGCG 1 cut(s) 263
BstKTI GATC 2 cut(s) 162, 280
BstMBI GATC 2 cut(s) 159, 277
BstMCI CGRYCG 1 cut(s) 347
BstMWI GCNNNNNNNGC 4 cut(s) 66, 101, 147, 307
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 89
BstSNI TACGTA 1 cut(s) 100
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 3 cut(s) 53, 294, 356
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BstZI CGGCCG 1 cut(s) 344
BsuRI GGCC 2 cut(s) 129, 346
BtgZI GCGATG 1 cut(s) 365
BtsIMutI CAGTG 1 cut(s) 150
Cac8I GCNNGC 2 cut(s) 344, 348
CaiI CAGNNNCTG 1 cut(s) 231
Cfr10I RCCGGY 4 cut(s) 91, 129, 342, 346
Cfr13I GGNCC 1 cut(s) 127
Csp6I GTAC 1 cut(s) 97
CviJI RGCY 5 cut(s) 34, 129, 301, 346, 369
CviKI_1 RGCY 5 cut(s) 34, 129, 301, 346, 369
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 1 cut(s) 153
DpnI GATC 2 cut(s) 161, 279
DpnII GATC 2 cut(s) 159, 277
EaeI YGGCCR 1 cut(s) 344
EagI CGGCCG 1 cut(s) 344
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
EciI GGCGGA 2 cut(s) 26, 290
EclXI CGGCCG 1 cut(s) 344
Eco105I TACGTA 1 cut(s) 100
Eco32I GATATC 1 cut(s) 285
Eco52I CGGCCG 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 123
EcoRV GATATC 1 cut(s) 285
FalI AAGNNNNNCTT 4 cut(s) 206, 238, 356, 388
FauI CCCGC 1 cut(s) 268
Fnu4HI GCNGC 5 cut(s) 67, 105, 218, 308, 370
Fsp4HI GCNGC 5 cut(s) 67, 105, 218, 308, 370
GluI GCNGC 5 cut(s) 67, 105, 218, 308, 370
HaeIII GGCC 2 cut(s) 129, 346
HapII CCGG 5 cut(s) 35, 92, 130, 343, 347
HinfI GANTC 6 cut(s) 5, 144, 228, 253, 313, 320
HpaII CCGG 5 cut(s) 35, 92, 130, 343, 347
HphI GGTGA 5 cut(s) 28, 64, 121, 223, 367
Hpy166II GTNNAC 1 cut(s) 87
Hpy188I TCNGA 4 cut(s) 31, 227, 246, 282
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 1 cut(s) 87
Hpy99I CGWCG 1 cut(s) 274
HpyAV CCTTC 3 cut(s) 70, 106, 382
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 3 cut(s) 87, 150, 234
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 101, 147, 307
HpyF3I CTNAG 1 cut(s) 153
HpySE526I ACGT 1 cut(s) 99
KroI GCCGGC 2 cut(s) 342, 346
KroNI GCCGGC 2 cut(s) 344, 348
Kzo9I GATC 2 cut(s) 159, 277
LguI GCTCTTC 1 cut(s) 359
LmnI GCTCC 1 cut(s) 31
LpnPI CCDG 9 cut(s) 48, 105, 110, 137, 143, 302, 356, 360, 380
Lsp1109I GCAGC 3 cut(s) 53, 294, 356
MaeII ACGT 1 cut(s) 99
MalI GATC 2 cut(s) 161, 279
MboI GATC 2 cut(s) 159, 277
MboII GAAGA 2 cut(s) 202, 376
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 89
MluCI AATT 1 cut(s) 400
MlyI GAGTC 4 cut(s) 14, 138, 307, 314
MroNI GCCGGC 2 cut(s) 342, 346
MspA1I CMGCKG 1 cut(s) 301
MspI CCGG 5 cut(s) 35, 92, 130, 343, 347
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 4 cut(s) 66, 101, 147, 307
NaeI GCCGGC 2 cut(s) 344, 348
NdeII GATC 2 cut(s) 159, 277
NgoMIV GCCGGC 2 cut(s) 342, 346
NlaIV GGNNCC 2 cut(s) 33, 122
NmeAIII GCCGAG 2 cut(s) 210, 351
PaeR7I CTCGAG 1 cut(s) 175
PciSI GCTCTTC 1 cut(s) 359
PcsI WCGNNNNNNNCGW 1 cut(s) 182
PdiI GCCGGC 2 cut(s) 344, 348
PfeI GAWTC 2 cut(s) 228, 253
Pfl23II CGTACG 1 cut(s) 96
PkrI GCNGC 5 cut(s) 68, 106, 219, 309, 371
PleI GAGTC 4 cut(s) 13, 138, 307, 314
PpsI GAGTC 4 cut(s) 13, 138, 307, 314
Ppu21I YACGTR 1 cut(s) 100
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspLI CGTACG 1 cut(s) 96
PspN4I GGNNCC 2 cut(s) 33, 122
PspPI GGNCC 1 cut(s) 127
PspXI VCTCGAGB 1 cut(s) 175
PstNI CAGNNNCTG 1 cut(s) 231
PsuI RGATCY 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 301
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SapI GCTCTTC 1 cut(s) 359
SatI GCNGC 5 cut(s) 67, 105, 218, 308, 370
Sau3AI GATC 2 cut(s) 159, 277
Sau96I GGNCC 1 cut(s) 127
SchI GAGTC 4 cut(s) 14, 138, 307, 314
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 1 cut(s) 89
SetI ASST 9 cut(s) 18, 45, 54, 102, 182, 303, 332, 362, 371
Sfr274I CTCGAG 1 cut(s) 175
SgrAI CRCCGGYG 1 cut(s) 91
SlaI CTCGAG 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 175
SmoI CTYRAG 1 cut(s) 175
SnaBI TACGTA 1 cut(s) 100
Sse9I AATT 1 cut(s) 400
StyD4I CCNGG 1 cut(s) 123
TaaI ACNGT 1 cut(s) 382
TaiI ACGT 1 cut(s) 102
TaqI TCGA 3 cut(s) 176, 251, 362
TasI AATT 1 cut(s) 400
TauI GCSGC 2 cut(s) 107, 220
TfiI GAWTC 2 cut(s) 228, 253
TscAI CASTG 1 cut(s) 157
TseI GCWGC 3 cut(s) 66, 307, 369
TspDTI ATGAA 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 215
TspRI CASTG 1 cut(s) 157
VneI GTGCAC 1 cut(s) 85
XhoI CTCGAG 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.