MD15G1047800.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002944
Physical Location & Seq
Forward (+)
3281589 .. 3282005
417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1047800.v1.1.491

Sequence Viewer

Length: 417 bp
ATGGAGTCGGCGGAGGCGAAACGGATTTCGGAGCCGGACGAGGAGGAGGTGGAGGTGAAGTGCTACTGCTGCGGTTTGACGGAGGAGTGCACGCCGGCATACGTAGCGACGGTGAAGGAGAGGCACCAGGACCGGTGGATTTGCGGGCTCTGCGCTGAGGCGATCAAGGACGAGAACTCGAGGTCGTCGAGGAAGATCAGCACGGAGGAGGCGATGAAGCGGCACGTCAGCTTCTGCGAGGAGTTCCGGATGTCGAGTCCTCCGGCGAAGCCGACGGAATATCTTATATCCGCGATGAAGCAACTGTTGCGGCGGACTAAGGATTCGCAGAGGAGGGAGAGACTGGGTGGCTGCCGGCCGGGGATGGTGAGGTCAAAGAGCTGCTTTGCGACGGTGGGGAAGGCGCAGGGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

139

Amino Acids

15.66

Weight (kDa)

8.07

Isoelectric Point (pI)

85.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 18 - 109 2.4e-36 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 123
AccII CGCG 1 cut(s) 293
AccIII TCCGGA 1 cut(s) 246
AciI CCGC 7 cut(s) 11, 72, 144, 220, 291, 310, 313
AcoI YGGCCR 1 cut(s) 356
AgeI ACCGGT 1 cut(s) 132
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 2 cut(s) 231, 381
AluI AGCT 2 cut(s) 231, 381
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 1 cut(s) 334
Alw44I GTGCAC 1 cut(s) 88
AlwNI CAGNNNCTG 1 cut(s) 234
Ama87I CYCGRG 1 cut(s) 178
Aor13HI TCCGGA 1 cut(s) 246
AoxI GGCC 1 cut(s) 356
ApaLI GTGCAC 1 cut(s) 88
ApeKI GCWGC 3 cut(s) 69, 351, 381
ArsI GACNNNNNNTTYG 2 cut(s) 307, 339
AsiGI ACCGGT 1 cut(s) 132
AspLEI GCGC 2 cut(s) 155, 406
AspS9I GGNCC 1 cut(s) 130
AsuC2I CCSGG 1 cut(s) 360
AsuHPI GGTGA 3 cut(s) 67, 124, 379
AvaI CYCGRG 1 cut(s) 178
AvaII GGWCC 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 92
BanI GGYRCC 1 cut(s) 123
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 92
BbvCI CCTCAGC 1 cut(s) 156
BbvI GCAGC 3 cut(s) 56, 338, 368
BccI CCATC 1 cut(s) 358
BciT130I CCWGG 1 cut(s) 128
BcnI CCSGG 1 cut(s) 360
BcoDI GTCTC 1 cut(s) 334
BisI GCNGC 5 cut(s) 70, 221, 311, 352, 382
BlsI GCNGC 5 cut(s) 71, 222, 312, 353, 383
Bme1390I CCNGG 2 cut(s) 128, 360
Bme18I GGWCC 1 cut(s) 130
BmeT110I CYCGRG 1 cut(s) 178
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 130
BmiI GGNNCC 2 cut(s) 33, 125
BmrFI CCNGG 2 cut(s) 128, 360
BmrI ACTGGG 1 cut(s) 353
BmuI ACTGGG 1 cut(s) 353
Bpu10I CCTNAGC 1 cut(s) 156
BpuMI CCSGG 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 359
BsaWI WCCGGW 2 cut(s) 132, 246
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bse118I RCCGGY 3 cut(s) 94, 132, 354
Bse1I ACTGG 1 cut(s) 348
BseAI TCCGGA 1 cut(s) 246
BseBI CCWGG 1 cut(s) 128
BseDI CCNNGG 1 cut(s) 359
BseGI GGATG 2 cut(s) 255, 369
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 1 cut(s) 348
BseRI GAGGAG 6 cut(s) 56, 59, 98, 221, 254, 346
BseSI GKGCMC 1 cut(s) 92
BseX3I CGGCCG 1 cut(s) 356
BseXI GCAGC 3 cut(s) 56, 338, 368
Bsh1236I CGCG 1 cut(s) 293
Bsh1285I CGRYCG 1 cut(s) 359
BshFI GGCC 1 cut(s) 358
BshNI GGYRCC 1 cut(s) 123
BshTI ACCGGT 1 cut(s) 132
BsiEI CGRYCG 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 92
BsiHKCI CYCGRG 1 cut(s) 178
BsiSI CCGG 7 cut(s) 35, 95, 133, 247, 263, 355, 359
BsmAI GTCTC 1 cut(s) 334
BsnI GGCC 1 cut(s) 358
BsoBI CYCGRG 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 92, 150
Bsp13I TCCGGA 1 cut(s) 246
Bsp143I GATC 2 cut(s) 162, 195
BspACI CCGC 7 cut(s) 11, 72, 144, 220, 291, 310, 313
BspANI GGCC 1 cut(s) 358
BspCNI CTCAG 1 cut(s) 148
BspEI TCCGGA 1 cut(s) 246
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 2 cut(s) 33, 125
BspT107I GGYRCC 1 cut(s) 123
BsrFI RCCGGY 3 cut(s) 94, 132, 354
BsrI ACTGG 1 cut(s) 348
BssAI RCCGGY 3 cut(s) 94, 132, 354
BssECI CCNNGG 1 cut(s) 359
BssMI GATC 2 cut(s) 162, 195
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 3 cut(s) 112, 306, 394
BstAPI GCANNNNNTGC 1 cut(s) 307
BstBAI YACGTR 1 cut(s) 103
BstC8I GCNNGC 4 cut(s) 92, 96, 146, 356
BstDEI CTNAG 2 cut(s) 156, 318
BstF5I GGATG 2 cut(s) 255, 369
BstFNI CGCG 1 cut(s) 293
BstHHI GCGC 2 cut(s) 155, 406
BstKTI GATC 2 cut(s) 165, 198
BstMAI GTCTC 1 cut(s) 334
BstMBI GATC 2 cut(s) 162, 195
BstMCI CGRYCG 1 cut(s) 359
BstMWI GCNNNNNNNGC 4 cut(s) 69, 104, 150, 307
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 2 cut(s) 126, 358
BstSLI GKGCMC 1 cut(s) 92
BstSNI TACGTA 1 cut(s) 103
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 3 cut(s) 56, 338, 368
BstZI CGGCCG 1 cut(s) 356
BsuRI GGCC 1 cut(s) 358
BtgZI GCGATG 2 cut(s) 227, 308
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 2 cut(s) 255, 369
Cac8I GCNNGC 4 cut(s) 92, 96, 146, 356
CaiI CAGNNNCTG 1 cut(s) 234
CfoI GCGC 2 cut(s) 155, 406
Cfr10I RCCGGY 3 cut(s) 94, 132, 354
Cfr13I GGNCC 1 cut(s) 130
CspAI ACCGGT 1 cut(s) 132
CviJI RGCY 7 cut(s) 34, 148, 231, 271, 351, 358, 381
CviKI_1 RGCY 7 cut(s) 34, 148, 231, 271, 351, 358, 381
DdeI CTNAG 2 cut(s) 156, 318
DpnI GATC 2 cut(s) 164, 197
DpnII GATC 2 cut(s) 162, 195
EaeI YGGCCR 1 cut(s) 356
EagI CGGCCG 1 cut(s) 356
EciI GGCGGA 2 cut(s) 26, 328
EclXI CGGCCG 1 cut(s) 356
Eco105I TACGTA 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 130
Eco52I CGGCCG 1 cut(s) 356
Eco88I CYCGRG 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 150
FaiI YATR 2 cut(s) 100, 287
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FauI CCCGC 1 cut(s) 137
Fnu4HI GCNGC 5 cut(s) 70, 221, 311, 352, 382
FokI GGATG 2 cut(s) 262, 376
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 5 cut(s) 70, 221, 311, 352, 382
GlaI GCGC 2 cut(s) 154, 405
GluI GCNGC 5 cut(s) 70, 221, 311, 352, 382
HaeIII GGCC 1 cut(s) 358
HapII CCGG 7 cut(s) 35, 95, 133, 247, 263, 355, 359
HhaI GCGC 2 cut(s) 155, 406
Hin6I GCGC 2 cut(s) 153, 404
HinP1I GCGC 2 cut(s) 153, 404
HinfI GANTC 3 cut(s) 5, 256, 323
HpaII CCGG 7 cut(s) 35, 95, 133, 247, 263, 355, 359
HphI GGTGA 3 cut(s) 67, 124, 379
Hpy166II GTNNAC 1 cut(s) 90
Hpy188I TCNGA 1 cut(s) 31
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 1 cut(s) 90
Hpy99I CGWCG 4 cut(s) 112, 190, 277, 394
HpyAV CCTTC 2 cut(s) 109, 394
HpyCH4III ACNGT 3 cut(s) 112, 306, 394
HpyCH4IV ACGT 2 cut(s) 102, 225
HpyCH4V TGCA 1 cut(s) 90
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 104, 150, 307
HpyF3I CTNAG 2 cut(s) 156, 318
HpySE526I ACGT 2 cut(s) 102, 225
HspAI GCGC 2 cut(s) 153, 404
Kpn2I TCCGGA 1 cut(s) 246
KroI GCCGGC 2 cut(s) 94, 354
KroNI GCCGGC 2 cut(s) 96, 356
Kzo9I GATC 2 cut(s) 162, 195
LmnI GCTCC 1 cut(s) 31
Lsp1109I GCAGC 3 cut(s) 56, 338, 368
MaeII ACGT 2 cut(s) 102, 225
MalI GATC 2 cut(s) 164, 197
MboI GATC 2 cut(s) 162, 195
MboII GAAGA 1 cut(s) 205
MhlI GDGCHC 2 cut(s) 92, 150
MlyI GAGTC 2 cut(s) 14, 265
MroI TCCGGA 1 cut(s) 246
MroNI GCCGGC 2 cut(s) 94, 354
MspI CCGG 7 cut(s) 35, 95, 133, 247, 263, 355, 359
MspR9I CCNGG 2 cut(s) 128, 360
MvaI CCWGG 1 cut(s) 128
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 4 cut(s) 69, 104, 150, 307
NaeI GCCGGC 2 cut(s) 96, 356
NciI CCSGG 1 cut(s) 360
NdeII GATC 2 cut(s) 162, 195
NgoMIV GCCGGC 2 cut(s) 94, 354
NlaIV GGNNCC 2 cut(s) 33, 125
PaeR7I CTCGAG 1 cut(s) 178
PcsI WCGNNNNNNNCGW 3 cut(s) 14, 185, 209
PdiI GCCGGC 2 cut(s) 96, 356
PfeI GAWTC 1 cut(s) 323
PinAI ACCGGT 1 cut(s) 132
PkrI GCNGC 5 cut(s) 71, 222, 312, 353, 383
PleI GAGTC 2 cut(s) 13, 264
PpsI GAGTC 2 cut(s) 13, 264
Ppu21I YACGTR 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 2 cut(s) 33, 125
PspPI GGNCC 1 cut(s) 130
PspXI VCTCGAGB 1 cut(s) 178
PstNI CAGNNNCTG 1 cut(s) 234
SatI GCNGC 5 cut(s) 70, 221, 311, 352, 382
Sau3AI GATC 2 cut(s) 162, 195
Sau96I GGNCC 1 cut(s) 130
SchI GAGTC 2 cut(s) 14, 265
ScrFI CCNGG 2 cut(s) 128, 360
SduI GDGCHC 2 cut(s) 92, 150
SetI ASST 8 cut(s) 51, 57, 105, 185, 228, 233, 374, 383
Sfr274I CTCGAG 1 cut(s) 178
SinI GGWCC 1 cut(s) 130
SlaI CTCGAG 1 cut(s) 178
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
SnaBI TACGTA 1 cut(s) 103
SsiI CCGC 7 cut(s) 11, 72, 144, 220, 291, 310, 313
StyD4I CCNGG 2 cut(s) 126, 358
TaaI ACNGT 3 cut(s) 112, 306, 394
TaiI ACGT 2 cut(s) 105, 228
TaqI TCGA 3 cut(s) 179, 188, 254
TauI GCSGC 2 cut(s) 223, 313
TfiI GAWTC 1 cut(s) 323
TseI GCWGC 3 cut(s) 69, 351, 381
TspDTI ATGAA 2 cut(s) 230, 311
TspGWI ACGGA 4 cut(s) 37, 95, 218, 290
VneI GTGCAC 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 130
XhoI CTCGAG 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.