Prupe.1G394900_v2.0.a1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0002944
Physical Location & Seq
Forward (+)
35083671 .. 35085310
1640 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G394900.1

Sequence Viewer

Length: 420 bp
ATGGAGGCGTCACAGCCAGGGAGTCCAAGCGCAGCTCAGTTGGTATTGGAAGAGGAGTTGGTGAAGTGCTACTGCTGCGGGTTGACGGAGGAATGCACGCTGGAGTACATAGCTACAGTGAAGGAGCGACATCAGGGCCGTTGGATTTGCGGACTTTGTGCGGAGGCGATCAAGGACGAGAACTCGAGGTCGCATAGGGGCCTCAGTGACGAGGAGGCGATGAAACGACACGCCAGTTTCCGTGATGAGTTTCGATTGTCGAGTCCTCCGACGAAGTCTGCTGAGGAATTGATATCGGCGATGAAGCAACTTTTGCGACGGACTTTGAACTCGTTGAAAAAAGAAGGGTTAGGTTGCCAACCCGCTCTTTCAAGGTCGAAGAGCTTCTTTGTGCATGTGGCAAAAGCGCAGGCAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

140

Amino Acids

15.49

Weight (kDa)

6.89

Isoelectric Point (pI)

66.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 365
AciI CCGC 4 cut(s) 78, 150, 161, 363
AcyI GRCGYC 1 cut(s) 8
AfaI GTAC 1 cut(s) 107
AgsI TTSAA 3 cut(s) 328, 337, 372
AjnI CCWGG 1 cut(s) 16
AluBI AGCT 3 cut(s) 35, 113, 384
AluI AGCT 3 cut(s) 35, 113, 384
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 2 cut(s) 136, 199
ApeKI GCWGC 2 cut(s) 32, 75
Asp700I GAANNNNTTC 1 cut(s) 383
AspLEI GCGC 2 cut(s) 32, 409
AspS9I GGNCC 2 cut(s) 136, 199
AsuHPI GGTGA 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 184
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 2 cut(s) 44, 62
BceAI ACGGC 1 cut(s) 123
BciT130I CCWGG 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 114
BisI GCNGC 2 cut(s) 33, 76
BlsI GCNGC 2 cut(s) 34, 77
Bme1390I CCNGG 1 cut(s) 18
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 136, 199
BmiI GGNNCC 1 cut(s) 200
BmrFI CCNGG 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 122
Bpu10I CCTNAGC 1 cut(s) 282
BsaHI GRCGYC 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 234
BseBI CCWGG 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 17
BseMII CTCAG 3 cut(s) 50, 217, 273
BseNI ACTGG 1 cut(s) 234
BseRI GAGGAG 2 cut(s) 68, 227
BseXI GCAGC 2 cut(s) 44, 62
BshFI GGCC 2 cut(s) 138, 201
BsiHKCI CYCGRG 1 cut(s) 184
BsmI GAATGC 1 cut(s) 98
BsnI GGCC 2 cut(s) 138, 201
BsoBI CYCGRG 1 cut(s) 184
Bsp143I GATC 1 cut(s) 168
BspACI CCGC 4 cut(s) 78, 150, 161, 363
BspANI GGCC 2 cut(s) 138, 201
BspCNI CTCAG 3 cut(s) 49, 216, 274
BspLI GGNNCC 1 cut(s) 200
BspQI GCTCTTC 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 365
BsrI ACTGG 1 cut(s) 234
BssECI CCNNGG 1 cut(s) 17
BssMI GATC 1 cut(s) 168
BssNI GRCGYC 1 cut(s) 8
Bst2UI CCWGG 1 cut(s) 18
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 2 cut(s) 45, 374
BstACI GRCGYC 1 cut(s) 8
BstAPI GCANNNNNTGC 1 cut(s) 313
BstC8I GCNNGC 2 cut(s) 98, 411
BstDEI CTNAG 3 cut(s) 36, 203, 282
BstHHI GCGC 2 cut(s) 32, 409
BstKTI GATC 1 cut(s) 171
BstMBI GATC 1 cut(s) 168
BstMWI GCNNNNNNNGC 2 cut(s) 75, 313
BstNI CCWGG 1 cut(s) 18
BstNSI RCATGY 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 16
BstSFI CTRYAG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 44, 62
BsuRI GGCC 2 cut(s) 138, 201
BtgZI GCGATG 2 cut(s) 233, 314
BtsIMutI CAGTG 2 cut(s) 123, 211
Cac8I GCNNGC 2 cut(s) 98, 411
CfoI GCGC 2 cut(s) 32, 409
Cfr13I GGNCC 2 cut(s) 136, 199
Csp6I GTAC 1 cut(s) 106
CviAII CATG 1 cut(s) 395
CviJI RGCY 6 cut(s) 16, 35, 113, 138, 201, 384
CviKI_1 RGCY 6 cut(s) 16, 35, 113, 138, 201, 384
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 3 cut(s) 36, 203, 282
DpnI GATC 1 cut(s) 170
DpnII GATC 1 cut(s) 168
Eam1104I CTCTTC 2 cut(s) 45, 374
EarI CTCTTC 2 cut(s) 45, 374
Eco32I GATATC 1 cut(s) 294
Eco88I CYCGRG 1 cut(s) 184
EcoO109I RGGNCCY 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 16
EcoRV GATATC 1 cut(s) 294
FaeI CATG 1 cut(s) 398
FaiI YATR 3 cut(s) 110, 195, 396
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FatI CATG 1 cut(s) 394
FauI CCCGC 2 cut(s) 71, 370
Fnu4HI GCNGC 2 cut(s) 33, 76
Fsp4HI GCNGC 2 cut(s) 33, 76
GlaI GCGC 2 cut(s) 31, 408
GluI GCNGC 2 cut(s) 33, 76
GsuI CTGGAG 1 cut(s) 122
HaeIII GGCC 2 cut(s) 138, 201
HhaI GCGC 2 cut(s) 32, 409
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 1 cut(s) 398
Hin6I GCGC 2 cut(s) 30, 407
HinP1I GCGC 2 cut(s) 30, 407
HincII GTYRAC 1 cut(s) 84
HindII GTYRAC 1 cut(s) 84
HinfI GANTC 2 cut(s) 22, 262
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 84
Hpy188I TCNGA 1 cut(s) 270
Hpy8I GTNNAC 1 cut(s) 84
Hpy99I CGWCG 2 cut(s) 274, 321
HpyAV CCTTC 2 cut(s) 115, 338
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4V TGCA 2 cut(s) 96, 394
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 313
HpyF3I CTNAG 3 cut(s) 36, 203, 282
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 1 cut(s) 398
HspAI GCGC 2 cut(s) 30, 407
Kzo9I GATC 1 cut(s) 168
LguI GCTCTTC 1 cut(s) 374
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 6 cut(s) 3, 30, 86, 119, 247, 395
Lsp1109I GCAGC 2 cut(s) 44, 62
MaeIII GTNAC 2 cut(s) 9, 206
MalI GATC 1 cut(s) 170
MbiI CCGCTC 1 cut(s) 365
MboI GATC 1 cut(s) 168
MboII GAAGA 2 cut(s) 62, 391
MluCI AATT 1 cut(s) 287
MlyI GAGTC 2 cut(s) 31, 271
MmeI TCCRAC 2 cut(s) 122, 293
MnlI CCTC 9 cut(s) 46, 82, 157, 180, 205, 208, 212, 276, 277
MroXI GAANNNNTTC 1 cut(s) 383
MspR9I CCNGG 1 cut(s) 18
Mva1269I GAATGC 1 cut(s) 98
MvaI CCWGG 1 cut(s) 18
MwoI GCNNNNNNNGC 2 cut(s) 75, 313
NdeII GATC 1 cut(s) 168
NlaIII CATG 1 cut(s) 398
NlaIV GGNNCC 1 cut(s) 200
NmuCI GTSAC 2 cut(s) 9, 206
NspI RCATGY 1 cut(s) 398
PaeR7I CTCGAG 1 cut(s) 184
PciSI GCTCTTC 1 cut(s) 374
PcsI WCGNNNNNNNCGW 1 cut(s) 266
PctI GAATGC 1 cut(s) 98
PdmI GAANNNNTTC 1 cut(s) 383
PflFI GACNNNGTC 1 cut(s) 274
PkrI GCNGC 2 cut(s) 34, 77
PleI GAGTC 2 cut(s) 30, 270
PpsI GAGTC 2 cut(s) 30, 270
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 200
PspPI GGNCC 2 cut(s) 136, 199
PspXI VCTCGAGB 1 cut(s) 184
PsyI GACNNNGTC 1 cut(s) 274
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SapI GCTCTTC 1 cut(s) 374
SatI GCNGC 2 cut(s) 33, 76
Sau3AI GATC 1 cut(s) 168
Sau96I GGNCC 2 cut(s) 136, 199
SchI GAGTC 2 cut(s) 31, 271
ScrFI CCNGG 1 cut(s) 18
SetI ASST 6 cut(s) 37, 115, 191, 355, 377, 386
SfcI CTRYAG 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 184
SlaI CTCGAG 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 184
SmoI CTYRAG 1 cut(s) 184
Sse9I AATT 1 cut(s) 287
SsiI CCGC 4 cut(s) 78, 150, 161, 363
StyD4I CCNGG 1 cut(s) 16
TaaI ACNGT 1 cut(s) 118
TaqI TCGA 4 cut(s) 185, 253, 260, 377
TasI AATT 1 cut(s) 287
TatI WGTACW 1 cut(s) 105
TscAI CASTG 2 cut(s) 123, 211
TseFI GTSAC 2 cut(s) 9, 206
TseI GCWGC 2 cut(s) 32, 75
Tsp45I GTSAC 2 cut(s) 9, 206
TspDTI ATGAA 2 cut(s) 236, 317
TspGWI ACGGA 3 cut(s) 101, 230, 334
TspRI CASTG 2 cut(s) 123, 211
Tth111I GACNNNGTC 1 cut(s) 274
XceI RCATGY 1 cut(s) 398
XhoI CTCGAG 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.