MD09G1180300.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002944
Physical Location & Seq
Forward (+)
15475775 .. 15477693
1919 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1180300.v1.1.491

Sequence Viewer

Length: 495 bp
ATGGCAATGTCCGCAACTATCGTCAGCACCGACACGATTGTTCTACCGGCCGCGGCCGCGGAAACTCAGCCACAACAAACCACAACAAAACTCGTTTCCCCCATCGAAATCGAGTTCGTACAATGCGACTGCTGTGGATTAACAGAAGAGTGCACACCTGCGTACATTGAGCACGTGAGGGAGAGATATCAGGGCCATTGGATTTGTGGGTTGTGTGCAGAAGCTATAAAAGATGAGATTGTTAGGTCGCAGAAGAGGCTGATCAGCACGGAAGAAGCCATGGCCACACACATGAACTTCTGCAAAAAGTTTAAGGACTCAGGGCCTCCTCCAAACCCTACCATTCACTTGATATCCGCCATGAGACAGATTCTCCGGCGCAGTTTGGATTCTCCGAGGGGGGGTTTGAGATCAACTCCAAGTAGTCCTACAAAGATAAATGCAGGCAAAGGGCTCACAAGGTCTGAGAGTTGCTTCCCGACTCTGACTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

165

Amino Acids

17.95

Weight (kDa)

7.57

Isoelectric Point (pI)

76.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 37 - 131 1.5e-41 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 166
Acc36I ACCTGC 1 cut(s) 166
AccII CGCG 2 cut(s) 53, 59
AciI CCGC 6 cut(s) 12, 51, 53, 57, 59, 357
AcoI YGGCCR 3 cut(s) 48, 54, 282
AcvI CACGTG 1 cut(s) 175
AfaI GTAC 2 cut(s) 120, 164
AfiI CCNNNNNNNGG 1 cut(s) 401
AluBI AGCT 1 cut(s) 224
AluI AGCT 1 cut(s) 224
Alw21I GWGCWC 2 cut(s) 155, 174
Alw26I GTCTC 1 cut(s) 358
Alw44I GTGCAC 1 cut(s) 151
AoxI GGCC 5 cut(s) 48, 54, 193, 282, 323
ApaLI GTGCAC 1 cut(s) 151
AspLEI GCGC 1 cut(s) 381
AspS9I GGNCC 2 cut(s) 193, 323
BaeGI GKGCMC 1 cut(s) 155
BalI TGGCCA 1 cut(s) 284
BanII GRGCYC 1 cut(s) 456
BbrPI CACGTG 1 cut(s) 175
Bbv12I GWGCWC 2 cut(s) 155, 174
BccI CCATC 1 cut(s) 110
BclI TGATCA 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 358
BfuAI ACCTGC 1 cut(s) 166
BisI GCNGC 3 cut(s) 51, 54, 57
BlsI GCNGC 3 cut(s) 52, 55, 58
BmgT120I GGNCC 2 cut(s) 193, 323
BplI GAGNNNNNCTC 2 cut(s) 400, 432
BsaAI YACGTR 1 cut(s) 175
BsaJI CCNNGG 4 cut(s) 51, 57, 279, 395
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 493
Bse3DI GCAATG 1 cut(s) 12
BseDI CCNNGG 4 cut(s) 51, 57, 279, 395
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 3 cut(s) 80, 333, 456
BseNI ACTGG 1 cut(s) 493
BseRI GAGGAG 1 cut(s) 318
BseSI GKGCMC 1 cut(s) 155
BseX3I CGGCCG 2 cut(s) 48, 54
BsgI GTGCAG 1 cut(s) 237
Bsh1236I CGCG 2 cut(s) 53, 59
Bsh1285I CGRYCG 2 cut(s) 51, 57
BshFI GGCC 5 cut(s) 50, 56, 195, 284, 325
BsiEI CGRYCG 2 cut(s) 51, 57
BsiHKAI GWGCWC 2 cut(s) 155, 174
BsiSI CCGG 2 cut(s) 47, 376
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 358
BsnI GGCC 5 cut(s) 50, 56, 195, 284, 325
Bsp1286I GDGCHC 3 cut(s) 155, 174, 456
Bsp143I GATC 2 cut(s) 261, 410
Bsp19I CCATGG 1 cut(s) 279
BspACI CCGC 6 cut(s) 12, 51, 53, 57, 59, 357
BspANI GGCC 5 cut(s) 50, 56, 195, 284, 325
BspCNI CTCAG 3 cut(s) 79, 332, 457
BspFNI CGCG 2 cut(s) 53, 59
BspMI ACCTGC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 46
BsrI ACTGG 1 cut(s) 493
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 4 cut(s) 51, 57, 279, 395
BssMI GATC 2 cut(s) 261, 410
BssT1I CCWWGG 1 cut(s) 279
Bst6I CTCTTC 2 cut(s) 141, 248
BstBAI YACGTR 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 445
BstDEI CTNAG 3 cut(s) 66, 319, 465
BstDSI CCRYGG 3 cut(s) 51, 57, 279
BstFNI CGCG 2 cut(s) 53, 59
BstHHI GCGC 1 cut(s) 381
BstKTI GATC 2 cut(s) 264, 413
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 2 cut(s) 261, 410
BstMCI CGRYCG 2 cut(s) 51, 57
BstMWI GCNNNNNNNGC 3 cut(s) 11, 56, 256
BstSLI GKGCMC 1 cut(s) 155
BstUI CGCG 2 cut(s) 53, 59
BstZI CGGCCG 2 cut(s) 48, 54
BsuRI GGCC 5 cut(s) 50, 56, 195, 284, 325
BtgI CCRYGG 3 cut(s) 51, 57, 279
BveI ACCTGC 1 cut(s) 166
Cac8I GCNNGC 1 cut(s) 445
CciNI GCGGCCGC 1 cut(s) 54
CfoI GCGC 1 cut(s) 381
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 2 cut(s) 193, 323
Cfr42I CCGCGG 2 cut(s) 54, 60
Csp6I GTAC 2 cut(s) 119, 163
CviAII CATG 3 cut(s) 280, 292, 361
CviQI GTAC 2 cut(s) 119, 163
DdeI CTNAG 3 cut(s) 66, 319, 465
DpnI GATC 2 cut(s) 263, 412
DpnII GATC 2 cut(s) 261, 410
EaeI YGGCCR 3 cut(s) 48, 54, 282
EagI CGGCCG 2 cut(s) 48, 54
Eam1104I CTCTTC 2 cut(s) 141, 248
EarI CTCTTC 2 cut(s) 141, 248
EciI GGCGGA 1 cut(s) 346
EclXI CGGCCG 2 cut(s) 48, 54
Eco130I CCWWGG 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 456
Eco32I GATATC 2 cut(s) 188, 354
Eco52I CGGCCG 2 cut(s) 48, 54
Eco72I CACGTG 1 cut(s) 175
EcoO109I RGGNCCY 1 cut(s) 323
EcoRV GATATC 2 cut(s) 188, 354
EcoT14I CCWWGG 1 cut(s) 279
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 3 cut(s) 283, 295, 364
FaiI YATR 4 cut(s) 227, 281, 293, 362
FatI CATG 3 cut(s) 279, 291, 360
FbaI TGATCA 1 cut(s) 261
Fnu4HI GCNGC 3 cut(s) 51, 54, 57
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 3 cut(s) 51, 54, 57
GlaI GCGC 1 cut(s) 380
GluI GCNGC 3 cut(s) 51, 54, 57
HaeIII GGCC 5 cut(s) 50, 56, 195, 284, 325
HapII CCGG 2 cut(s) 47, 376
HhaI GCGC 1 cut(s) 381
Hin1II CATG 3 cut(s) 283, 295, 364
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HinfI GANTC 4 cut(s) 317, 370, 389, 481
HpaII CCGG 2 cut(s) 47, 376
Hpy166II GTNNAC 1 cut(s) 153
Hpy188I TCNGA 3 cut(s) 396, 466, 486
Hpy188III TCNNGA 1 cut(s) 478
Hpy8I GTNNAC 1 cut(s) 153
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 4 cut(s) 153, 218, 303, 443
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 56, 256
HpyF3I CTNAG 3 cut(s) 66, 319, 465
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 3 cut(s) 283, 295, 364
HspAI GCGC 1 cut(s) 379
Ksp22I TGATCA 1 cut(s) 261
KspI CCGCGG 2 cut(s) 54, 60
Kzo9I GATC 2 cut(s) 261, 410
LpnPI CCDG 7 cut(s) 60, 171, 176, 306, 389, 429, 474
MaeII ACGT 1 cut(s) 174
MalI GATC 2 cut(s) 263, 412
MboI GATC 2 cut(s) 261, 410
MboII GAAGA 3 cut(s) 158, 265, 284
MhlI GDGCHC 3 cut(s) 155, 174, 456
MlsI TGGCCA 1 cut(s) 284
MluNI TGGCCA 1 cut(s) 284
MlyI GAGTC 2 cut(s) 311, 475
MnlI CCTC 5 cut(s) 171, 249, 336, 339, 390
Mox20I TGGCCA 1 cut(s) 284
MscI TGGCCA 1 cut(s) 284
MseI TTAA 2 cut(s) 140, 312
MslI CAYNNNNRTG 1 cut(s) 290
Msp20I TGGCCA 1 cut(s) 284
MspA1I CMGCKG 2 cut(s) 53, 59
MspI CCGG 2 cut(s) 47, 376
MvnI CGCG 2 cut(s) 53, 59
MwoI GCNNNNNNNGC 3 cut(s) 11, 56, 256
NcoI CCATGG 1 cut(s) 279
NdeII GATC 2 cut(s) 261, 410
NlaIII CATG 3 cut(s) 283, 295, 364
NotI GCGGCCGC 1 cut(s) 54
PaqCI CACCTGC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 2 cut(s) 27, 123
PfeI GAWTC 2 cut(s) 370, 389
PkrI GCNGC 3 cut(s) 52, 55, 58
PleI GAGTC 2 cut(s) 311, 475
PmaCI CACGTG 1 cut(s) 175
PmlI CACGTG 1 cut(s) 175
PpsI GAGTC 2 cut(s) 311, 475
Ppu21I YACGTR 1 cut(s) 175
PspCI CACGTG 1 cut(s) 175
PspPI GGNCC 2 cut(s) 193, 323
RsaI GTAC 2 cut(s) 120, 164
RsaNI GTAC 2 cut(s) 119, 163
RseI CAYNNNNRTG 1 cut(s) 290
SacII CCGCGG 2 cut(s) 54, 60
SaqAI TTAA 2 cut(s) 140, 312
SatI GCNGC 3 cut(s) 51, 54, 57
Sau3AI GATC 2 cut(s) 261, 410
Sau96I GGNCC 2 cut(s) 193, 323
SchI GAGTC 2 cut(s) 311, 475
SduI GDGCHC 3 cut(s) 155, 174, 456
SetI ASST 5 cut(s) 160, 177, 226, 248, 464
Sfr303I CCGCGG 2 cut(s) 54, 60
SgrBI CCGCGG 2 cut(s) 54, 60
SmiMI CAYNNNNRTG 1 cut(s) 290
SsiI CCGC 6 cut(s) 12, 51, 53, 57, 59, 357
StyI CCWWGG 1 cut(s) 279
TaiI ACGT 1 cut(s) 177
TaqI TCGA 2 cut(s) 105, 111
TauI GCSGC 3 cut(s) 53, 56, 59
TfiI GAWTC 2 cut(s) 370, 389
Tru1I TTAA 2 cut(s) 140, 312
Tru9I TTAA 2 cut(s) 140, 312
TspDTI ATGAA 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 284
VneI GTGCAC 1 cut(s) 151
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.