MD06G1201300.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002944
Physical Location & Seq
Reverse (-)
33439172 .. 33439609
438 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1201300.v1.1.491

Sequence Viewer

Length: 438 bp
ATGCTCATGGCCCGGTCGGAGGTGAAGTGCTACTGCTGCGGTTTGACGGAGGAGTGCACGCCGGCATACGTAGCGACGGTGAAGGAGAGGCACCAGGACCAGTGGATTTGCGGGCTCTGCGCTGAGGCGATCGAGGACGAGAACTCGAGGTCGTCGAGGAAGATCAACACGGAGGAGGCGATGAAGCGGCACGTCAGCTTCTGCGAGGAGTTCCGGATGTCGAGTCCTCCGGCGAAGCCGACAGAATGTCTTATATCCGCGACGAAGCAACTGTTGCGGCGGACTAAGGATTCGCAGGGGAGGGAGATACTGGGTGGCTGCCGGCCGGGAATGGTGAGGTCAAAGAGCTGTTTTGCGACGGTGGGGAAGGTGCCAAGGGTCGAAAACCACATCGGTGGAGATGAGCCGGACCGTGTTAAGGGAATTCCTGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

146

Amino Acids

16.24

Weight (kDa)

7.5

Isoelectric Point (pI)

87.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 7 - 98 9e-34 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 90, 370
AccII CGCG 1 cut(s) 260
AccIII TCCGGA 1 cut(s) 213
AciI CCGC 6 cut(s) 39, 111, 187, 258, 277, 280
AcoI YGGCCR 1 cut(s) 323
AcsI RAATTY 1 cut(s) 423
AfiI CCNNNNNNNGG 2 cut(s) 19, 418
AhdI GACNNNNNGTC 1 cut(s) 246
AjiI CACGTC 1 cut(s) 193
AjnI CCWGG 1 cut(s) 93
AleI CACNNNNGTG 1 cut(s) 393
AluBI AGCT 2 cut(s) 198, 348
AluI AGCT 2 cut(s) 198, 348
Alw21I GWGCWC 1 cut(s) 59
Alw44I GTGCAC 1 cut(s) 55
AlwNI CAGNNNCTG 1 cut(s) 201
Ama87I CYCGRG 1 cut(s) 145
Aor13HI TCCGGA 1 cut(s) 213
AoxI GGCC 2 cut(s) 9, 323
ApaLI GTGCAC 1 cut(s) 55
ApeKI GCWGC 2 cut(s) 36, 318
ApoI RAATTY 1 cut(s) 423
ArsI GACNNNNNNTTYG 2 cut(s) 274, 306
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 3 cut(s) 10, 97, 409
AsuC2I CCSGG 2 cut(s) 13, 327
AsuHPI GGTGA 3 cut(s) 34, 91, 346
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 2 cut(s) 97, 409
BaeGI GKGCMC 1 cut(s) 59
BanI GGYRCC 2 cut(s) 90, 370
BanII GRGCYC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 59
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 23, 305
BciT130I CCWGG 1 cut(s) 95
BcnI CCSGG 2 cut(s) 13, 327
BisI GCNGC 4 cut(s) 37, 188, 278, 319
BlsI GCNGC 4 cut(s) 38, 189, 279, 320
Bme1390I CCNGG 3 cut(s) 13, 95, 327
Bme18I GGWCC 2 cut(s) 97, 409
BmeRI GACNNNNNGTC 1 cut(s) 246
BmeT110I CYCGRG 1 cut(s) 145
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 3 cut(s) 10, 97, 409
BmiI GGNNCC 2 cut(s) 92, 372
BmrFI CCNGG 3 cut(s) 13, 95, 327
BmrI ACTGGG 1 cut(s) 320
BmuI ACTGGG 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 123
BpuMI CCSGG 2 cut(s) 13, 327
BsaAI YACGTR 1 cut(s) 70
BsaJI CCNNGG 1 cut(s) 374
BsaWI WCCGGW 1 cut(s) 213
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 418
Bse118I RCCGGY 2 cut(s) 61, 321
Bse1I ACTGG 2 cut(s) 100, 315
BseAI TCCGGA 1 cut(s) 213
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 1 cut(s) 374
BseGI GGATG 1 cut(s) 222
BseLI CCNNNNNNNGG 2 cut(s) 19, 418
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 2 cut(s) 100, 315
BseRI GAGGAG 3 cut(s) 65, 188, 221
BseSI GKGCMC 1 cut(s) 59
BseX3I CGGCCG 1 cut(s) 323
BseXI GCAGC 2 cut(s) 23, 305
Bsh1236I CGCG 1 cut(s) 260
Bsh1285I CGRYCG 3 cut(s) 17, 132, 326
BshFI GGCC 2 cut(s) 11, 325
BshNI GGYRCC 2 cut(s) 90, 370
BsiEI CGRYCG 3 cut(s) 17, 132, 326
BsiHKAI GWGCWC 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 7 cut(s) 13, 62, 214, 230, 322, 326, 407
BslI CCNNNNNNNGG 2 cut(s) 19, 418
BsnI GGCC 2 cut(s) 11, 325
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 59, 117
Bsp13I TCCGGA 1 cut(s) 213
Bsp143I GATC 2 cut(s) 129, 162
BspACI CCGC 6 cut(s) 39, 111, 187, 258, 277, 280
BspANI GGCC 2 cut(s) 11, 325
BspCNI CTCAG 1 cut(s) 115
BspEI TCCGGA 1 cut(s) 213
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 2 cut(s) 92, 372
BspT107I GGYRCC 2 cut(s) 90, 370
BsrFI RCCGGY 2 cut(s) 61, 321
BsrI ACTGG 2 cut(s) 100, 315
BssAI RCCGGY 2 cut(s) 61, 321
BssECI CCNNGG 1 cut(s) 374
BssMI GATC 2 cut(s) 129, 162
BssT1I CCWWGG 1 cut(s) 374
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 4 cut(s) 79, 273, 361, 413
BstAPI GCANNNNNTGC 1 cut(s) 274
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 4 cut(s) 59, 63, 113, 323
BstDEI CTNAG 2 cut(s) 123, 285
BstF5I GGATG 1 cut(s) 222
BstFNI CGCG 1 cut(s) 260
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 2 cut(s) 132, 165
BstMBI GATC 2 cut(s) 129, 162
BstMCI CGRYCG 3 cut(s) 17, 132, 326
BstMWI GCNNNNNNNGC 4 cut(s) 36, 71, 117, 274
BstNI CCWGG 1 cut(s) 95
BstSCI CCNGG 3 cut(s) 11, 93, 325
BstSLI GKGCMC 1 cut(s) 59
BstSNI TACGTA 1 cut(s) 70
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 2 cut(s) 23, 305
BstXI CCANNNNNNTGG 1 cut(s) 395
BstZI CGGCCG 1 cut(s) 323
BsuRI GGCC 2 cut(s) 11, 325
BtgZI GCGATG 1 cut(s) 194
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 107
Cac8I GCNNGC 4 cut(s) 59, 63, 113, 323
CaiI CAGNNNCTG 1 cut(s) 201
CfoI GCGC 1 cut(s) 122
Cfr10I RCCGGY 2 cut(s) 61, 321
Cfr13I GGNCC 3 cut(s) 10, 97, 409
CpoI CGGWCCG 1 cut(s) 409
CspI CGGWCCG 1 cut(s) 409
CviAII CATG 1 cut(s) 7
CviJI RGCY 8 cut(s) 11, 115, 198, 238, 318, 325, 348, 406
CviKI_1 RGCY 8 cut(s) 11, 115, 198, 238, 318, 325, 348, 406
DdeI CTNAG 2 cut(s) 123, 285
DpnI GATC 2 cut(s) 131, 164
DpnII GATC 2 cut(s) 129, 162
DriI GACNNNNNGTC 1 cut(s) 246
EaeI YGGCCR 1 cut(s) 323
EagI CGGCCG 1 cut(s) 323
Eam1105I GACNNNNNGTC 1 cut(s) 246
EciI GGCGGA 1 cut(s) 295
EclXI CGGCCG 1 cut(s) 323
Eco105I TACGTA 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 374
Eco24I GRGCYC 1 cut(s) 117
Eco47I GGWCC 2 cut(s) 97, 409
Eco52I CGGCCG 1 cut(s) 323
Eco88I CYCGRG 1 cut(s) 145
EcoRI GAATTC 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 374
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 374
FaeI CATG 1 cut(s) 10
FaiI YATR 3 cut(s) 8, 67, 254
FatI CATG 1 cut(s) 6
FauI CCCGC 1 cut(s) 104
Fnu4HI GCNGC 4 cut(s) 37, 188, 278, 319
FokI GGATG 1 cut(s) 229
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 4 cut(s) 37, 188, 278, 319
GlaI GCGC 1 cut(s) 121
GluI GCNGC 4 cut(s) 37, 188, 278, 319
HaeIII GGCC 2 cut(s) 11, 325
HapII CCGG 7 cut(s) 13, 62, 214, 230, 322, 326, 407
HhaI GCGC 1 cut(s) 122
Hin1II CATG 1 cut(s) 10
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 2 cut(s) 223, 290
HpaII CCGG 7 cut(s) 13, 62, 214, 230, 322, 326, 407
HphI GGTGA 3 cut(s) 34, 91, 346
Hpy166II GTNNAC 1 cut(s) 57
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 2 cut(s) 214, 428
Hpy8I GTNNAC 1 cut(s) 57
Hpy99I CGWCG 4 cut(s) 79, 157, 265, 361
HpyAV CCTTC 2 cut(s) 76, 361
HpyCH4III ACNGT 4 cut(s) 79, 273, 361, 413
HpyCH4IV ACGT 2 cut(s) 69, 192
HpyCH4V TGCA 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 71, 117, 274
HpyF3I CTNAG 2 cut(s) 123, 285
HpySE526I ACGT 2 cut(s) 69, 192
Hsp92II CATG 1 cut(s) 10
HspAI GCGC 1 cut(s) 120
Kpn2I TCCGGA 1 cut(s) 213
KroI GCCGGC 2 cut(s) 61, 321
KroNI GCCGGC 2 cut(s) 63, 323
Kzo9I GATC 2 cut(s) 129, 162
Lsp1109I GCAGC 2 cut(s) 23, 305
MaeII ACGT 2 cut(s) 69, 192
MalI GATC 2 cut(s) 131, 164
MboI GATC 2 cut(s) 129, 162
MboII GAAGA 1 cut(s) 172
MhlI GDGCHC 2 cut(s) 59, 117
MluCI AATT 1 cut(s) 423
MlyI GAGTC 1 cut(s) 232
MroI TCCGGA 1 cut(s) 213
MroNI GCCGGC 2 cut(s) 61, 321
MseI TTAA 1 cut(s) 417
MslI CAYNNNNRTG 1 cut(s) 393
MspI CCGG 7 cut(s) 13, 62, 214, 230, 322, 326, 407
MspR9I CCNGG 3 cut(s) 13, 95, 327
MvaI CCWGG 1 cut(s) 95
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 4 cut(s) 36, 71, 117, 274
NaeI GCCGGC 2 cut(s) 63, 323
NciI CCSGG 2 cut(s) 13, 327
NdeII GATC 2 cut(s) 129, 162
NgoMIV GCCGGC 2 cut(s) 61, 321
NlaIII CATG 1 cut(s) 10
NlaIV GGNNCC 2 cut(s) 92, 372
OliI CACNNNNGTG 1 cut(s) 393
PaeR7I CTCGAG 1 cut(s) 145
PcsI WCGNNNNNNNCGW 2 cut(s) 152, 176
PdiI GCCGGC 2 cut(s) 63, 323
PfeI GAWTC 1 cut(s) 290
PkrI GCNGC 4 cut(s) 38, 189, 279, 320
Ple19I CGATCG 1 cut(s) 132
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
Ppu21I YACGTR 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 2 cut(s) 92, 372
PspPI GGNCC 3 cut(s) 10, 97, 409
PspXI VCTCGAGB 1 cut(s) 145
PstNI CAGNNNCTG 1 cut(s) 201
PvuI CGATCG 1 cut(s) 132
RseI CAYNNNNRTG 1 cut(s) 393
Rsr2I CGGWCCG 1 cut(s) 409
RsrII CGGWCCG 1 cut(s) 409
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 4 cut(s) 37, 188, 278, 319
Sau3AI GATC 2 cut(s) 129, 162
Sau96I GGNCC 3 cut(s) 10, 97, 409
SchI GAGTC 1 cut(s) 232
ScrFI CCNGG 3 cut(s) 13, 95, 327
SduI GDGCHC 2 cut(s) 59, 117
SetI ASST 8 cut(s) 24, 72, 152, 195, 200, 341, 350, 372
Sfr274I CTCGAG 1 cut(s) 145
SinI GGWCC 2 cut(s) 97, 409
SlaI CTCGAG 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 393
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
SnaBI TACGTA 1 cut(s) 70
Sse9I AATT 1 cut(s) 423
SsiI CCGC 6 cut(s) 39, 111, 187, 258, 277, 280
StyD4I CCNGG 3 cut(s) 11, 93, 325
StyI CCWWGG 1 cut(s) 374
TaaI ACNGT 4 cut(s) 79, 273, 361, 413
TaiI ACGT 2 cut(s) 72, 195
TaqI TCGA 5 cut(s) 132, 146, 155, 221, 381
TasI AATT 1 cut(s) 423
TauI GCSGC 2 cut(s) 190, 280
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 1 cut(s) 107
TseI GCWGC 2 cut(s) 36, 318
TspDTI ATGAA 1 cut(s) 197
TspGWI ACGGA 2 cut(s) 62, 185
TspRI CASTG 1 cut(s) 107
VneI GTGCAC 1 cut(s) 55
VpaK11BI GGWCC 2 cut(s) 97, 409
XapI RAATTY 1 cut(s) 423
XhoI CTCGAG 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.