MD04G1143800.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002944
Physical Location & Seq
Reverse (-)
23295673 .. 23296020
348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1143800.v1.1.491

Sequence Viewer

Length: 348 bp
ATGCTCATGGCCCGGTCGGAGGTGAAGTGCTACTGCTGCGGTTTGACGGAGGAGTGCAGGCCGGCATACGTAGCGACGGTGAAGGAGAGGCACCAGGACCGGTGGATTTACGGGTTCTGCGTTGAGGCGATCAAGGACGAGAACTCGAGGTCGTCGAGGAAGATAAGCACGGAGGAGGCGATGAAGCAGCACGTCAGCTTTTGCGAGGATTTCCGGATGTCGAGTCCTCCGGCGAAGCCGACGGAATATCTTATAGCTGCGAAGCAACTGTTGCGGCGGACTAAGGATTCGCAGATGAGGGAGAGACTGGGTGGCTGCCGGCCGGGGATGGTGACTTGGGGAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

116

Amino Acids

13.26

Weight (kDa)

8.9

Isoelectric Point (pI)

73.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 7 - 97 2.2e-29 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 90
AccIII TCCGGA 1 cut(s) 213
AciI CCGC 3 cut(s) 39, 274, 277
AcoI YGGCCR 1 cut(s) 320
AfiI CCNNNNNNNGG 1 cut(s) 19
AgeI ACCGGT 1 cut(s) 99
AjiI CACGTC 1 cut(s) 193
AjnI CCWGG 1 cut(s) 93
AluBI AGCT 2 cut(s) 198, 257
AluI AGCT 2 cut(s) 198, 257
Alw26I GTCTC 1 cut(s) 298
Ama87I CYCGRG 1 cut(s) 145
Aor13HI TCCGGA 1 cut(s) 213
AoxI GGCC 3 cut(s) 9, 59, 320
ApeKI GCWGC 4 cut(s) 36, 187, 257, 315
ArsI GACNNNNNNTTYG 2 cut(s) 271, 303
AsiGI ACCGGT 1 cut(s) 99
AspS9I GGNCC 2 cut(s) 10, 97
AsuC2I CCSGG 2 cut(s) 13, 324
AsuHPI GGTGA 3 cut(s) 34, 91, 343
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 1 cut(s) 97
BanI GGYRCC 1 cut(s) 90
BbvI GCAGC 4 cut(s) 23, 199, 244, 302
BccI CCATC 1 cut(s) 322
BciT130I CCWGG 1 cut(s) 95
BcnI CCSGG 2 cut(s) 13, 324
BcoDI GTCTC 1 cut(s) 298
BisI GCNGC 5 cut(s) 37, 188, 258, 275, 316
BlsI GCNGC 5 cut(s) 38, 189, 259, 276, 317
Bme1390I CCNGG 3 cut(s) 13, 95, 324
Bme18I GGWCC 1 cut(s) 97
BmeT110I CYCGRG 1 cut(s) 145
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 2 cut(s) 10, 97
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 3 cut(s) 13, 95, 324
BmrI ACTGGG 1 cut(s) 317
BmuI ACTGGG 1 cut(s) 317
BpuMI CCSGG 2 cut(s) 13, 324
BsaAI YACGTR 1 cut(s) 70
BsaJI CCNNGG 1 cut(s) 323
BsaWI WCCGGW 2 cut(s) 99, 213
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 1 cut(s) 19
Bse118I RCCGGY 3 cut(s) 61, 99, 318
Bse1I ACTGG 1 cut(s) 312
BseAI TCCGGA 1 cut(s) 213
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 1 cut(s) 323
BseGI GGATG 2 cut(s) 222, 333
BseLI CCNNNNNNNGG 1 cut(s) 19
BseNI ACTGG 1 cut(s) 312
BseRI GAGGAG 2 cut(s) 65, 188
BseX3I CGGCCG 1 cut(s) 320
BseXI GCAGC 4 cut(s) 23, 199, 244, 302
BsgI GTGCAG 1 cut(s) 76
Bsh1285I CGRYCG 2 cut(s) 17, 323
BshFI GGCC 3 cut(s) 11, 61, 322
BshNI GGYRCC 1 cut(s) 90
BshTI ACCGGT 1 cut(s) 99
BsiEI CGRYCG 2 cut(s) 17, 323
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 7 cut(s) 13, 62, 100, 214, 230, 319, 323
BslI CCNNNNNNNGG 1 cut(s) 19
BsmAI GTCTC 1 cut(s) 298
BsnI GGCC 3 cut(s) 11, 61, 322
BsoBI CYCGRG 1 cut(s) 145
Bsp13I TCCGGA 1 cut(s) 213
Bsp143I GATC 1 cut(s) 129
BspACI CCGC 3 cut(s) 39, 274, 277
BspANI GGCC 3 cut(s) 11, 61, 322
BspEI TCCGGA 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 90
BsrFI RCCGGY 3 cut(s) 61, 99, 318
BsrI ACTGG 1 cut(s) 312
BssAI RCCGGY 3 cut(s) 61, 99, 318
BssECI CCNNGG 1 cut(s) 323
BssMI GATC 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 2 cut(s) 79, 270
BstAPI GCANNNNNTGC 1 cut(s) 271
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 3 cut(s) 59, 63, 320
BstDEI CTNAG 1 cut(s) 282
BstF5I GGATG 2 cut(s) 222, 333
BstKTI GATC 1 cut(s) 132
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 1 cut(s) 129
BstMCI CGRYCG 2 cut(s) 17, 323
BstMWI GCNNNNNNNGC 3 cut(s) 36, 71, 271
BstNI CCWGG 1 cut(s) 95
BstSCI CCNGG 3 cut(s) 11, 93, 322
BstSNI TACGTA 1 cut(s) 70
BstV1I GCAGC 4 cut(s) 23, 199, 244, 302
BstZI CGGCCG 1 cut(s) 320
BsuRI GGCC 3 cut(s) 11, 61, 322
BtgZI GCGATG 1 cut(s) 194
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 2 cut(s) 222, 333
Cac8I GCNNGC 3 cut(s) 59, 63, 320
Cfr10I RCCGGY 3 cut(s) 61, 99, 318
Cfr13I GGNCC 2 cut(s) 10, 97
CspAI ACCGGT 1 cut(s) 99
CviAII CATG 1 cut(s) 7
CviJI RGCY 7 cut(s) 11, 61, 198, 238, 257, 315, 322
CviKI_1 RGCY 7 cut(s) 11, 61, 198, 238, 257, 315, 322
DdeI CTNAG 1 cut(s) 282
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
EaeI YGGCCR 1 cut(s) 320
EagI CGGCCG 1 cut(s) 320
EciI GGCGGA 1 cut(s) 292
EclXI CGGCCG 1 cut(s) 320
Eco105I TACGTA 1 cut(s) 70
Eco47I GGWCC 1 cut(s) 97
Eco52I CGGCCG 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 93
FaeI CATG 1 cut(s) 10
FaiI YATR 3 cut(s) 8, 67, 254
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 5 cut(s) 37, 188, 258, 275, 316
FokI GGATG 2 cut(s) 229, 340
Fsp4HI GCNGC 5 cut(s) 37, 188, 258, 275, 316
GluI GCNGC 5 cut(s) 37, 188, 258, 275, 316
HaeIII GGCC 3 cut(s) 11, 61, 322
HapII CCGG 7 cut(s) 13, 62, 100, 214, 230, 319, 323
Hin1II CATG 1 cut(s) 10
HinfI GANTC 2 cut(s) 223, 287
HpaII CCGG 7 cut(s) 13, 62, 100, 214, 230, 319, 323
HphI GGTGA 3 cut(s) 34, 91, 343
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 1 cut(s) 214
Hpy99I CGWCG 3 cut(s) 79, 157, 244
HpyAV CCTTC 1 cut(s) 76
HpyCH4III ACNGT 2 cut(s) 79, 270
HpyCH4IV ACGT 2 cut(s) 69, 192
HpyCH4V TGCA 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 71, 271
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 2 cut(s) 69, 192
Hsp92II CATG 1 cut(s) 10
Kpn2I TCCGGA 1 cut(s) 213
KroI GCCGGC 2 cut(s) 61, 318
KroNI GCCGGC 2 cut(s) 63, 320
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 1 cut(s) 341
Lsp1109I GCAGC 4 cut(s) 23, 199, 244, 302
MaeII ACGT 2 cut(s) 69, 192
MaeIII GTNAC 1 cut(s) 331
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 1 cut(s) 172
MlyI GAGTC 1 cut(s) 232
MroI TCCGGA 1 cut(s) 213
MroNI GCCGGC 2 cut(s) 61, 318
MspI CCGG 7 cut(s) 13, 62, 100, 214, 230, 319, 323
MspR9I CCNGG 3 cut(s) 13, 95, 324
MvaI CCWGG 1 cut(s) 95
MwoI GCNNNNNNNGC 3 cut(s) 36, 71, 271
NaeI GCCGGC 2 cut(s) 63, 320
NciI CCSGG 2 cut(s) 13, 324
NdeII GATC 1 cut(s) 129
NgoMIV GCCGGC 2 cut(s) 61, 318
NlaIII CATG 1 cut(s) 10
NlaIV GGNNCC 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 331
PaeR7I CTCGAG 1 cut(s) 145
PcsI WCGNNNNNNNCGW 3 cut(s) 117, 152, 176
PdiI GCCGGC 2 cut(s) 63, 320
PfeI GAWTC 1 cut(s) 287
PinAI ACCGGT 1 cut(s) 99
PkrI GCNGC 5 cut(s) 38, 189, 259, 276, 317
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
Ppu21I YACGTR 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 2 cut(s) 10, 97
PspXI VCTCGAGB 1 cut(s) 145
SatI GCNGC 5 cut(s) 37, 188, 258, 275, 316
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 2 cut(s) 10, 97
SchI GAGTC 1 cut(s) 232
ScrFI CCNGG 3 cut(s) 13, 95, 324
SetI ASST 6 cut(s) 24, 72, 152, 195, 200, 259
Sfr274I CTCGAG 1 cut(s) 145
SinI GGWCC 1 cut(s) 97
SlaI CTCGAG 1 cut(s) 145
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
SnaBI TACGTA 1 cut(s) 70
SsiI CCGC 3 cut(s) 39, 274, 277
StyD4I CCNGG 3 cut(s) 11, 93, 322
TaaI ACNGT 2 cut(s) 79, 270
TaiI ACGT 2 cut(s) 72, 195
TaqI TCGA 3 cut(s) 146, 155, 221
TauI GCSGC 1 cut(s) 277
TfiI GAWTC 1 cut(s) 287
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 4 cut(s) 36, 187, 257, 315
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 1 cut(s) 197
TspGWI ACGGA 3 cut(s) 62, 185, 257
VpaK11BI GGWCC 1 cut(s) 97
XhoI CTCGAG 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.