pycom08g03800

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002944
Physical Location & Seq
Reverse (-)
2902349 .. 2902780
432 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03800.1

Sequence Viewer

Length: 432 bp
ATGAGAGGTTTTGTTATGGAGTCGGCGGAGGCGAAGCGGGTTTCGGAGCCGCAGGAGGAGGTGGTGGTGGTGAAGTGCTACTGCTGCGGTTTGGCGGAGGAGTGCACACCGGCGTACATAGCGACGGTGAAGGAGAGGCACCAGGGCCGGTGGATTTGCGGACTCTGCACTGAGGCGATCAAGGACGAGAACTCGAGGTCACCGAGGAAGATTAGCACGGAGGAGGCGATGAAGCGGCACGTCAGCTTCTACGAGGAGTTTCGGATGTCGAGTCCTCCCGCGAACCCGACGGAGGATCTGATATCGGCGGTGAAACAGCTGTTGCGGCGGACTCTGAACTCGCCGAGGAGGGAGAGGCTGGGCGACTGCCGGCCGGCGATGGTGAGGTCGAAGAGCTGCTTTGCGACTGTCGGGAAGGCACTGGGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

144

Amino Acids

16.13

Weight (kDa)

8.83

Isoelectric Point (pI)

87.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 23 - 114 7.1e-37 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 138
AccII CGCG 1 cut(s) 281
AclWI GGATC 1 cut(s) 303
AcoI YGGCCR 1 cut(s) 371
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 292
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 3 cut(s) 246, 319, 396
AluI AGCT 3 cut(s) 246, 319, 396
Alw21I GWGCWC 1 cut(s) 107
Alw44I GTGCAC 1 cut(s) 103
AlwI GGATC 1 cut(s) 303
Ama87I CYCGRG 1 cut(s) 193
AoxI GGCC 2 cut(s) 145, 371
ApaLI GTGCAC 1 cut(s) 103
ApeKI GCWGC 2 cut(s) 84, 396
AspS9I GGNCC 1 cut(s) 145
AsuHPI GGTGA 5 cut(s) 82, 139, 192, 322, 394
AvaI CYCGRG 1 cut(s) 193
BaeGI GKGCMC 1 cut(s) 107
BanI GGYRCC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 71, 383
BccI CCATC 1 cut(s) 373
BciT130I CCWGG 1 cut(s) 143
BisI GCNGC 5 cut(s) 50, 85, 236, 326, 397
BlsI GCNGC 5 cut(s) 51, 86, 237, 327, 398
Bme1390I CCNGG 1 cut(s) 143
BmeT110I CYCGRG 1 cut(s) 193
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 2 cut(s) 48, 140
BmrFI CCNGG 1 cut(s) 143
BmrI ACTGGG 1 cut(s) 431
BmuI ACTGGG 1 cut(s) 431
BsaJI CCNNGG 3 cut(s) 142, 203, 344
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse118I RCCGGY 4 cut(s) 109, 147, 369, 373
Bse1I ACTGG 1 cut(s) 426
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 3 cut(s) 142, 203, 344
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 1 cut(s) 292
BseMII CTCAG 1 cut(s) 162
BseNI ACTGG 1 cut(s) 426
BseRI GAGGAG 5 cut(s) 71, 113, 236, 269, 361
BseSI GKGCMC 1 cut(s) 107
BseX3I CGGCCG 1 cut(s) 371
BseXI GCAGC 2 cut(s) 71, 383
BseYI CCCAGC 1 cut(s) 358
BsgI GTGCAG 1 cut(s) 151
Bsh1236I CGCG 1 cut(s) 281
Bsh1285I CGRYCG 1 cut(s) 374
BshFI GGCC 2 cut(s) 147, 373
BshNI GGYRCC 1 cut(s) 138
BsiEI CGRYCG 1 cut(s) 374
BsiHKAI GWGCWC 1 cut(s) 107
BsiHKCI CYCGRG 1 cut(s) 193
BsiSI CCGG 4 cut(s) 110, 148, 370, 374
BslI CCNNNNNNNGG 1 cut(s) 292
BsnI GGCC 2 cut(s) 147, 373
BsoBI CYCGRG 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 107
Bsp143I GATC 2 cut(s) 177, 295
BspANI GGCC 2 cut(s) 147, 373
BspCNI CTCAG 1 cut(s) 163
BspFNI CGCG 1 cut(s) 281
BspLI GGNNCC 2 cut(s) 48, 140
BspPI GGATC 1 cut(s) 303
BspQI GCTCTTC 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 138
BsrFI RCCGGY 4 cut(s) 109, 147, 369, 373
BsrI ACTGG 1 cut(s) 426
BssAI RCCGGY 4 cut(s) 109, 147, 369, 373
BssECI CCNNGG 3 cut(s) 142, 203, 344
BssMI GATC 2 cut(s) 177, 295
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 127, 409
Bst6I CTCTTC 1 cut(s) 386
BstC8I GCNNGC 2 cut(s) 371, 375
BstDEI CTNAG 1 cut(s) 171
BstEII GGTNACC 1 cut(s) 198
BstF5I GGATG 1 cut(s) 270
BstFNI CGCG 1 cut(s) 281
BstKTI GATC 2 cut(s) 180, 298
BstMBI GATC 2 cut(s) 177, 295
BstMCI CGRYCG 1 cut(s) 374
BstMWI GCNNNNNNNGC 4 cut(s) 84, 119, 165, 325
BstNI CCWGG 1 cut(s) 143
BstPI GGTNACC 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 141
BstSLI GKGCMC 1 cut(s) 107
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 71, 383
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BstZI CGGCCG 1 cut(s) 371
BsuRI GGCC 2 cut(s) 147, 373
BtgZI GCGATG 2 cut(s) 242, 392
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 1 cut(s) 270
BtsIMutI CAGTG 2 cut(s) 168, 419
Cac8I GCNNGC 2 cut(s) 371, 375
Cfr10I RCCGGY 4 cut(s) 109, 147, 369, 373
Cfr13I GGNCC 1 cut(s) 145
Csp6I GTAC 1 cut(s) 115
CviJI RGCY 7 cut(s) 49, 147, 246, 319, 358, 373, 396
CviKI_1 RGCY 7 cut(s) 49, 147, 246, 319, 358, 373, 396
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 1 cut(s) 171
DpnI GATC 2 cut(s) 179, 297
DpnII GATC 2 cut(s) 177, 295
EaeI YGGCCR 1 cut(s) 371
EagI CGGCCG 1 cut(s) 371
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
EciI GGCGGA 3 cut(s) 41, 110, 343
EclXI CGGCCG 1 cut(s) 371
Eco32I GATATC 1 cut(s) 303
Eco52I CGGCCG 1 cut(s) 371
Eco88I CYCGRG 1 cut(s) 193
Eco91I GGTNACC 1 cut(s) 198
EcoO65I GGTNACC 1 cut(s) 198
EcoRII CCWGG 1 cut(s) 141
EcoRV GATATC 1 cut(s) 303
FaiI YATR 2 cut(s) 17, 119
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FauI CCCGC 2 cut(s) 30, 286
Fnu4HI GCNGC 5 cut(s) 50, 85, 236, 326, 397
FokI GGATG 1 cut(s) 277
Fsp4HI GCNGC 5 cut(s) 50, 85, 236, 326, 397
GluI GCNGC 5 cut(s) 50, 85, 236, 326, 397
GsaI CCCAGC 1 cut(s) 362
HaeIII GGCC 2 cut(s) 147, 373
HapII CCGG 4 cut(s) 110, 148, 370, 374
HinfI GANTC 4 cut(s) 20, 162, 271, 331
HpaII CCGG 4 cut(s) 110, 148, 370, 374
HphI GGTGA 5 cut(s) 82, 139, 192, 322, 394
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 4 cut(s) 46, 264, 300, 336
Hpy188III TCNNGA 1 cut(s) 412
Hpy8I GTNNAC 1 cut(s) 105
Hpy99I CGWCG 2 cut(s) 127, 292
HpyAV CCTTC 2 cut(s) 124, 409
HpyCH4III ACNGT 2 cut(s) 127, 409
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 2 cut(s) 105, 168
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 119, 165, 325
HpyF3I CTNAG 1 cut(s) 171
HpySE526I ACGT 1 cut(s) 240
KroI GCCGGC 2 cut(s) 369, 373
KroNI GCCGGC 2 cut(s) 371, 375
Kzo9I GATC 2 cut(s) 177, 295
LguI GCTCTTC 1 cut(s) 386
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 9 cut(s) 38, 123, 128, 155, 161, 344, 383, 387, 407
Lsp1109I GCAGC 2 cut(s) 71, 383
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 198
MalI GATC 2 cut(s) 179, 297
MboI GATC 2 cut(s) 177, 295
MboII GAAGA 2 cut(s) 220, 403
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 1 cut(s) 427
MlyI GAGTC 4 cut(s) 29, 156, 280, 325
MroNI GCCGGC 2 cut(s) 369, 373
MspA1I CMGCKG 1 cut(s) 319
MspI CCGG 4 cut(s) 110, 148, 370, 374
MspR9I CCNGG 1 cut(s) 143
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 4 cut(s) 84, 119, 165, 325
NaeI GCCGGC 2 cut(s) 371, 375
NdeII GATC 2 cut(s) 177, 295
NgoMIV GCCGGC 2 cut(s) 369, 373
NlaIV GGNNCC 2 cut(s) 48, 140
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 198
PaeR7I CTCGAG 1 cut(s) 193
PciSI GCTCTTC 1 cut(s) 386
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 200, 224
PdiI GCCGGC 2 cut(s) 371, 375
PkrI GCNGC 5 cut(s) 51, 86, 237, 327, 398
PleI GAGTC 4 cut(s) 28, 156, 279, 325
PpsI GAGTC 4 cut(s) 28, 156, 279, 325
Psp6I CCWGG 1 cut(s) 141
PspEI GGTNACC 1 cut(s) 198
PspFI CCCAGC 1 cut(s) 358
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 2 cut(s) 48, 140
PspPI GGNCC 1 cut(s) 145
PspXI VCTCGAGB 1 cut(s) 193
PsuI RGATCY 1 cut(s) 295
PvuII CAGCTG 1 cut(s) 319
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
SapI GCTCTTC 1 cut(s) 386
SatI GCNGC 5 cut(s) 50, 85, 236, 326, 397
Sau3AI GATC 2 cut(s) 177, 295
Sau96I GGNCC 1 cut(s) 145
SchI GAGTC 4 cut(s) 29, 156, 280, 325
ScrFI CCNGG 1 cut(s) 143
SduI GDGCHC 1 cut(s) 107
SetI ASST 8 cut(s) 10, 63, 200, 243, 248, 321, 389, 398
Sfr274I CTCGAG 1 cut(s) 193
SgrAI CRCCGGYG 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 193
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 1 cut(s) 427
StyD4I CCNGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 127, 409
TaiI ACGT 1 cut(s) 243
TaqI TCGA 3 cut(s) 194, 269, 389
TasI AATT 1 cut(s) 427
TauI GCSGC 3 cut(s) 52, 238, 328
TscAI CASTG 2 cut(s) 175, 426
TseFI GTSAC 1 cut(s) 198
TseI GCWGC 2 cut(s) 84, 396
Tsp45I GTSAC 1 cut(s) 198
TspDTI ATGAA 1 cut(s) 245
TspGWI ACGGA 2 cut(s) 233, 305
TspRI CASTG 2 cut(s) 175, 426
VneI GTGCAC 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.