FvH4_3g01580

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002602
Physical Location & Seq
Forward (+)
809970 .. 813584
3615 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g01580.t1

Sequence Viewer

Length: 1164 bp
ATGGTGACGGCGAAGAAGCCGCCGCCGCCGATCACGGCGCGCCACGTCATCAGGAGAAGCTGGAAGCTTCTGATCGTGTTCTCCGTCGCGCTCTGCCTTTTGGCTCTGTACAGGCTTCACTCCCAGCCGGACCTGTACTCTCCTTCTTCCTCATTATCTCGCGCGAGATCTCGCATCGCTCGCCACAGCGTCGGCTTCGCCGGCCCGGCCAAGATCGCCTTCCTCTTCCTCGCGCGCCGTGACCTCCCGCTCGATTTCCTCTGGGAGAGCTTCTTCGAGAATGCCGGTGGCGCTTTGAATTTTTCGATTTATATTCACTCCGCGCCTGGTTTTGTGTTCGATGAGTCCACCACGAGGTCGCGCTTCTTTCACGGCCGTCAATTGCCGAATAGCATTCAGGTGGGATGGGGGGAATCGAGCATGATTGAAGCGGAGAGGTTGTTGTTTGCGACAGCTCTTGAGGATCCAGCGAATCAAAGATTCGTTCTCCTCTCTGACAGTTGTGTTCCTTTGTACAACTTCAGCTTCATATACAATTACCTGATGGCTTCTCCTGGGAGCATTGTGGACAGCTTCCTGGATGTGAAGGAGGGCCGCTACAACCCAAAAATGTCACCCATAATACCGAAAAAGAAATGGCGGAAAGGATCACAGTGGATTGCTTTGATTCGGAGACACGCTGAAGTCCTTGTGGATGACGAAGTTATACTTCCAGTATTCAAGAAATTTTGCAGGCGTCGCCCACCTGTTGATATCAGGAAGGGGAAGCTGAATCTTAAACTTCAAAAACAGCACAATTGTATCCCAGACGAACACTATGTGCAGACACTGCTTGCGATGAGTGAGCTTGATGGCGAACTTGAGCGAAGGACATTAACTTATACATTGTGGAACAACTCTCCAAATAAAACAGAAAGTAAAGGCTGGCATCCTATGACTTTCAACCATGCGAGTGCAGCCCCTCAAAAGATAAGGGAAATAAAGGAGATCAACCATGTATACTATGAGACAGAATACCGAACAGAATGGTGTCGTATAAATTCTACTGCTGTTCCCTGTTTTCTATTCGCGAGGAAGTTCTCCAAGGGTGCTGCCATGCGCCTTCTCAGTGAGGGAGTTGTTGGTCACTTTGACACAGCTGCATTGTTAAAACCATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

388

Amino Acids

44.32

Weight (kDa)

9.71

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 72 - 327 5.5e-68 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 250
AccI GTMKAC 1 cut(s) 999
AccII CGCG 9 cut(s) 40, 89, 162, 164, 233, 235, 323, 361, 1070
AciI CCGC 8 cut(s) 20, 23, 26, 248, 321, 431, 595, 640
AclWI GGATC 3 cut(s) 458, 471, 655
AcoI YGGCCR 2 cut(s) 207, 373
AcsI RAATTY 3 cut(s) 298, 725, 1039
AcuI CTGAAG 2 cut(s) 505, 702
AcyI GRCGYC 1 cut(s) 736
AdeI CACNNNGTG 1 cut(s) 820
AfaI GTAC 3 cut(s) 110, 137, 515
AfiI CCNNNNNNNGG 1 cut(s) 354
AgsI TTSAA 5 cut(s) 298, 428, 721, 785, 943
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 3 cut(s) 325, 553, 576
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AluBI AGCT 9 cut(s) 60, 67, 270, 455, 525, 573, 769, 847, 1139
AluI AGCT 9 cut(s) 60, 67, 270, 455, 525, 573, 769, 847, 1139
Alw26I GTCTC 2 cut(s) 667, 1001
AlwI GGATC 3 cut(s) 458, 471, 655
AlwNI CAGNNNCTG 1 cut(s) 829
AoxI GGCC 4 cut(s) 202, 207, 373, 592
ApeKI GCWGC 3 cut(s) 956, 1091, 1139
ApoI RAATTY 3 cut(s) 298, 725, 1039
AscI GGCGCGCC 1 cut(s) 38
AspS9I GGNCC 3 cut(s) 130, 203, 592
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 3 cut(s) 16, 606, 1149
AvaII GGWCC 1 cut(s) 130
BaeI ACNNNNGTAYC 2 cut(s) 784, 817
BamHI GGATCC 1 cut(s) 463
BauI CACGAG 1 cut(s) 352
BbvI GCAGC 3 cut(s) 968, 1078, 1126
BccI CCATC 4 cut(s) 399, 538, 845, 1162
BceAI ACGGC 5 cut(s) 24, 51, 222, 360, 388
BciT130I CCWGG 3 cut(s) 327, 555, 578
BciVI GTATCC 1 cut(s) 812
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 2 cut(s) 667, 1001
BfoI RGCGCY 1 cut(s) 294
BfuI GTATCC 1 cut(s) 812
BglI GCCNNNNNGGC 1 cut(s) 206
BglII AGATCT 1 cut(s) 167
BisI GCNGC 7 cut(s) 20, 23, 26, 595, 957, 1092, 1140
BlsI GCNGC 7 cut(s) 21, 24, 27, 596, 958, 1093, 1141
Bme1390I CCNGG 4 cut(s) 206, 327, 555, 578
Bme18I GGWCC 1 cut(s) 130
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 3 cut(s) 130, 203, 592
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 4 cut(s) 206, 327, 555, 578
BmsI GCATC 2 cut(s) 183, 937
BpuEI CTTGAG 2 cut(s) 479, 881
BpuMI CCSGG 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 736
BsaJI CCNNGG 2 cut(s) 554, 1083
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse118I RCCGGY 2 cut(s) 200, 284
Bse1I ACTGG 1 cut(s) 713
BseBI CCWGG 3 cut(s) 327, 555, 578
BseDI CCNNGG 2 cut(s) 554, 1083
BseGI GGATG 4 cut(s) 410, 586, 700, 928
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMII CTCAG 1 cut(s) 1120
BseNI ACTGG 1 cut(s) 713
BsePI GCGCGC 2 cut(s) 38, 233
BseRI GAGGAG 1 cut(s) 479
BseX3I CGGCCG 1 cut(s) 373
BseXI GCAGC 3 cut(s) 968, 1078, 1126
BseYI CCCAGC 1 cut(s) 123
BsgI GTGCAG 2 cut(s) 842, 975
Bsh1236I CGCG 9 cut(s) 40, 89, 162, 164, 233, 235, 323, 361, 1070
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 4 cut(s) 204, 209, 375, 594
BsiEI CGRYCG 1 cut(s) 376
BsiSI CCGG 4 cut(s) 128, 201, 206, 285
BslI CCNNNNNNNGG 1 cut(s) 354
BsmAI GTCTC 2 cut(s) 667, 1001
BsmI GAATGC 2 cut(s) 286, 393
BsnI GGCC 4 cut(s) 204, 209, 375, 594
Bsp1407I TGTACA 2 cut(s) 108, 513
Bsp143I GATC 7 cut(s) 30, 72, 167, 213, 463, 647, 987
Bsp68I TCGCGA 1 cut(s) 1070
BspACI CCGC 8 cut(s) 20, 23, 26, 248, 321, 431, 595, 640
BspANI GGCC 4 cut(s) 204, 209, 375, 594
BspCNI CTCAG 1 cut(s) 1119
BspFNI CGCG 9 cut(s) 40, 89, 162, 164, 233, 235, 323, 361, 1070
BspLI GGNNCC 1 cut(s) 465
BspPI GGATC 3 cut(s) 458, 471, 655
BsrBI CCGCTC 1 cut(s) 250
BsrFI RCCGGY 2 cut(s) 200, 284
BsrGI TGTACA 2 cut(s) 108, 513
BsrI ACTGG 1 cut(s) 713
BssAI RCCGGY 2 cut(s) 200, 284
BssECI CCNNGG 2 cut(s) 554, 1083
BssHII GCGCGC 2 cut(s) 38, 233
BssMI GATC 7 cut(s) 30, 72, 167, 213, 463, 647, 987
BssNAI GTATAC 1 cut(s) 1000
BssNI GRCGYC 1 cut(s) 736
BssSI CACGAG 1 cut(s) 352
BssT1I CCWWGG 1 cut(s) 1083
Bst1107I GTATAC 1 cut(s) 1000
Bst2BI CACGAG 1 cut(s) 352
Bst2UI CCWGG 3 cut(s) 327, 555, 578
Bst4CI ACNGT 2 cut(s) 500, 654
Bst6I CTCTTC 1 cut(s) 230
BstACI GRCGYC 1 cut(s) 736
BstAPI GCANNNNNTGC 1 cut(s) 829
BstAUI TGTACA 2 cut(s) 108, 513
BstC8I GCNNGC 7 cut(s) 40, 181, 202, 235, 734, 834, 926
BstDEI CTNAG 1 cut(s) 1106
BstF5I GGATG 4 cut(s) 410, 586, 700, 928
BstFNI CGCG 9 cut(s) 40, 89, 162, 164, 233, 235, 323, 361, 1070
BstH2I RGCGCY 1 cut(s) 294
BstKTI GATC 7 cut(s) 33, 75, 170, 216, 466, 650, 990
BstMAI GTCTC 2 cut(s) 667, 1001
BstMBI GATC 7 cut(s) 30, 72, 167, 213, 463, 647, 987
BstMCI CGRYCG 1 cut(s) 376
BstMWI GCNNNNNNNGC 9 cut(s) 25, 180, 201, 206, 215, 290, 738, 829, 956
BstNI CCWGG 3 cut(s) 327, 555, 578
BstSCI CCNGG 4 cut(s) 204, 325, 553, 576
BstUI CGCG 9 cut(s) 40, 89, 162, 164, 233, 235, 323, 361, 1070
BstV1I GCAGC 3 cut(s) 968, 1078, 1126
BstX2I RGATCY 2 cut(s) 167, 463
BstYI RGATCY 2 cut(s) 167, 463
BstZ17I GTATAC 1 cut(s) 1000
BstZI CGGCCG 1 cut(s) 373
BsuI GTATCC 1 cut(s) 812
BsuRI GGCC 4 cut(s) 204, 209, 375, 594
BtgZI GCGATG 2 cut(s) 160, 851
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 4 cut(s) 410, 586, 700, 928
BtsI GCAGTG 1 cut(s) 827
BtsIMutI CAGTG 3 cut(s) 659, 827, 1114
BtuMI TCGCGA 1 cut(s) 1070
Cac8I GCNNGC 7 cut(s) 40, 181, 202, 235, 734, 834, 926
CaiI CAGNNNCTG 1 cut(s) 829
Cfr10I RCCGGY 2 cut(s) 200, 284
Cfr13I GGNCC 3 cut(s) 130, 203, 592
CseI GACGC 2 cut(s) 178, 725
Csp6I GTAC 3 cut(s) 109, 136, 514
CviAII CATG 5 cut(s) 421, 947, 995, 1096, 1161
CviQI GTAC 3 cut(s) 109, 136, 514
DdeI CTNAG 1 cut(s) 1106
DpnI GATC 7 cut(s) 32, 74, 169, 215, 465, 649, 989
DpnII GATC 7 cut(s) 30, 72, 167, 213, 463, 647, 987
DraIII CACNNNGTG 1 cut(s) 820
EaeI YGGCCR 2 cut(s) 207, 373
EagI CGGCCG 1 cut(s) 373
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
EciI GGCGGA 1 cut(s) 655
EclXI CGGCCG 1 cut(s) 373
Eco130I CCWWGG 1 cut(s) 1083
Eco32I GATATC 1 cut(s) 754
Eco47I GGWCC 1 cut(s) 130
Eco52I CGGCCG 1 cut(s) 373
Eco57I CTGAAG 2 cut(s) 505, 702
EcoRII CCWGG 3 cut(s) 325, 553, 576
EcoRV GATATC 1 cut(s) 754
EcoT14I CCWWGG 1 cut(s) 1083
ErhI CCWWGG 1 cut(s) 1083
FaeI CATG 5 cut(s) 424, 950, 998, 1099, 1164
FalI AAGNNNNNCTT 4 cut(s) 203, 235, 693, 725
FatI CATG 5 cut(s) 420, 946, 994, 1095, 1160
FauI CCCGC 1 cut(s) 255
FblI GTMKAC 1 cut(s) 999
Fnu4HI GCNGC 7 cut(s) 20, 23, 26, 595, 957, 1092, 1140
FokI GGATG 4 cut(s) 417, 593, 707, 915
Fsp4HI GCNGC 7 cut(s) 20, 23, 26, 595, 957, 1092, 1140
GluI GCNGC 7 cut(s) 20, 23, 26, 595, 957, 1092, 1140
GsaI CCCAGC 1 cut(s) 127
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 4 cut(s) 204, 209, 375, 594
HapII CCGG 4 cut(s) 128, 201, 206, 285
HgaI GACGC 2 cut(s) 178, 725
Hin1I GRCGYC 1 cut(s) 736
Hin1II CATG 5 cut(s) 424, 950, 998, 1099, 1164
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 6 cut(s) 344, 413, 472, 480, 667, 772
HpaII CCGG 4 cut(s) 128, 201, 206, 285
HphI GGTGA 3 cut(s) 16, 606, 1149
Hpy166II GTNNAC 3 cut(s) 348, 568, 1000
Hpy188I TCNGA 3 cut(s) 72, 496, 672
Hpy188III TCNNGA 6 cut(s) 52, 277, 458, 721, 757, 1069
Hpy8I GTNNAC 3 cut(s) 348, 568, 1000
Hpy99I CGWCG 3 cut(s) 89, 194, 741
HpyAV CCTTC 6 cut(s) 153, 229, 580, 754, 861, 1112
HpyCH4III ACNGT 2 cut(s) 500, 654
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 4 cut(s) 732, 823, 956, 1142
HpyF10VI GCNNNNNNNGC 9 cut(s) 25, 180, 201, 206, 215, 290, 738, 829, 956
HpyF3I CTNAG 1 cut(s) 1106
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 736
Hsp92II CATG 5 cut(s) 424, 950, 998, 1099, 1164
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Kzo9I GATC 7 cut(s) 30, 72, 167, 213, 463, 647, 987
LmnI GCTCC 1 cut(s) 558
Lsp1109I GCAGC 3 cut(s) 968, 1078, 1126
LweI GCATC 2 cut(s) 183, 937
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 4 cut(s) 4, 239, 612, 1124
MalI GATC 7 cut(s) 32, 74, 169, 215, 465, 649, 989
MbiI CCGCTC 1 cut(s) 250
MboI GATC 7 cut(s) 30, 72, 167, 213, 463, 647, 987
MboII GAAGA 4 cut(s) 25, 138, 217, 265
MfeI CAATTG 2 cut(s) 380, 796
MflI RGATCY 2 cut(s) 167, 463
MluCI AATT 6 cut(s) 298, 380, 535, 725, 796, 1039
MlyI GAGTC 1 cut(s) 353
MroNI GCCGGC 1 cut(s) 200
MseI TTAA 3 cut(s) 777, 875, 1148
MslI CAYNNNNRTG 3 cut(s) 398, 951, 1159
MspA1I CMGCKG 1 cut(s) 1139
MspI CCGG 4 cut(s) 128, 201, 206, 285
MspR9I CCNGG 4 cut(s) 206, 327, 555, 578
MunI CAATTG 2 cut(s) 380, 796
Mva1269I GAATGC 2 cut(s) 286, 393
MvaI CCWGG 3 cut(s) 327, 555, 578
MvnI CGCG 9 cut(s) 40, 89, 162, 164, 233, 235, 323, 361, 1070
MwoI GCNNNNNNNGC 9 cut(s) 25, 180, 201, 206, 215, 290, 738, 829, 956
NaeI GCCGGC 1 cut(s) 202
NciI CCSGG 1 cut(s) 206
NdeII GATC 7 cut(s) 30, 72, 167, 213, 463, 647, 987
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 5 cut(s) 424, 950, 998, 1099, 1164
NlaIV GGNNCC 1 cut(s) 465
NmuCI GTSAC 4 cut(s) 4, 239, 612, 1124
NruI TCGCGA 1 cut(s) 1070
PalAI GGCGCGCC 1 cut(s) 38
PauI GCGCGC 2 cut(s) 38, 233
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 2 cut(s) 286, 393
PdiI GCCGGC 1 cut(s) 202
PfeI GAWTC 5 cut(s) 413, 472, 480, 667, 772
PfoI TCCNGGA 1 cut(s) 576
PkrI GCNGC 7 cut(s) 21, 24, 27, 596, 958, 1093, 1141
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
Psp6I CCWGG 3 cut(s) 325, 553, 576
PspFI CCCAGC 1 cut(s) 123
PspGI CCWGG 3 cut(s) 325, 553, 576
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 3 cut(s) 130, 203, 592
PstNI CAGNNNCTG 1 cut(s) 829
PsuI RGATCY 2 cut(s) 167, 463
PteI GCGCGC 2 cut(s) 38, 233
PvuII CAGCTG 1 cut(s) 1139
RruI TCGCGA 1 cut(s) 1070
RsaI GTAC 3 cut(s) 110, 137, 515
RsaNI GTAC 3 cut(s) 109, 136, 514
RseI CAYNNNNRTG 3 cut(s) 398, 951, 1159
SaqAI TTAA 3 cut(s) 777, 875, 1148
SatI GCNGC 7 cut(s) 20, 23, 26, 595, 957, 1092, 1140
Sau3AI GATC 7 cut(s) 30, 72, 167, 213, 463, 647, 987
Sau96I GGNCC 3 cut(s) 130, 203, 592
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 4 cut(s) 206, 327, 555, 578
SfaNI GCATC 2 cut(s) 183, 937
SgsI GGCGCGCC 1 cut(s) 38
SinI GGWCC 1 cut(s) 130
SmiMI CAYNNNNRTG 3 cut(s) 398, 951, 1159
SmlI CTYRAG 2 cut(s) 458, 860
SmoI CTYRAG 2 cut(s) 458, 860
Sse9I AATT 6 cut(s) 298, 380, 535, 725, 796, 1039
SsiI CCGC 8 cut(s) 20, 23, 26, 248, 321, 431, 595, 640
StyD4I CCNGG 4 cut(s) 204, 325, 553, 576
StyI CCWWGG 1 cut(s) 1083
TaaI ACNGT 2 cut(s) 500, 654
TaiI ACGT 1 cut(s) 48
TaqI TCGA 5 cut(s) 252, 276, 305, 339, 416
TasI AATT 6 cut(s) 298, 380, 535, 725, 796, 1039
TatI WGTACW 3 cut(s) 108, 135, 513
TauI GCSGC 4 cut(s) 22, 25, 28, 597
TfiI GAWTC 5 cut(s) 413, 472, 480, 667, 772
Tru1I TTAA 3 cut(s) 777, 875, 1148
Tru9I TTAA 3 cut(s) 777, 875, 1148
TscAI CASTG 3 cut(s) 659, 834, 1114
TseFI GTSAC 4 cut(s) 4, 239, 612, 1124
TseI GCWGC 3 cut(s) 956, 1091, 1139
Tsp45I GTSAC 4 cut(s) 4, 239, 612, 1124
TspDTI ATGAA 1 cut(s) 517
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 3 cut(s) 659, 834, 1114
VpaK11BI GGWCC 1 cut(s) 130
XapI RAATTY 3 cut(s) 298, 725, 1039
XmiI GTMKAC 1 cut(s) 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.