MD05G1350900.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002602
Physical Location & Seq
Reverse (-)
46808317 .. 46811839
3523 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1350900.v1.1.491

Sequence Viewer

Length: 1407 bp
ATGATCACGGCGCGGAAATCAGAGACTCCACACTTGTCATCATTGTCCGCTGACAGAGTCCAGCAGAGGAGTCCACCTTCTCCCTCTTTCTCTCCGAAGCCCCACATAAAACTCCAAAATTCACACACCCAGCAGCGCACCCAGCGCACCCCCTCCCCTCTCCTCCTCTTCCTACTATTTGAAAATTCAAATGCTCCTCCTCTCCTCTCCTCCTCCGAATTCAACCCTTCAATGACGATGACGATGATGACGATGAAGAAGTCACCGCCGATCCCGGCGCGCCACGTCATCAGGTTGAGCTGGAAGCTGGTGATCGTCCTCTCCGTCGCGCTTTGCGTATGGGCCTTGTTCCGGCTCCACTCGCAGCCCGACCTCATTTCCTCTTCTCCGTCTTCGTTGGTCGTAGCGAGATCTCGAATTTCCCGCCACGGCAGAAGCTTCACCGGCACTCCGAAGATCGCGTTCCTTTTCCTAGCCCGCCGTAGCCTCCCGCTCGATTTTCTCTGGGGCAGCTTCTTCGAGAATGCTGAAGCGCCGAATTTTTCGATTTACATTCACTCGGCGCCTGGTTTTGTGTTCGATGAGTCGACGACGCGGTCTCATTTCTTTTACGGCCGTCAATTGACGAATAGCATACAGGTGGGTTGGGGAGAATCGAGTATGATCGAAGCGGAGAGGCTGTTGTTTGCAACAGCTCTTGGGGATCCCGCAAATCAAAGATTTATACTCCTTTCTGATAGTTGTGTTCCTTTGTACAACTTCAGCTATGTATACAGTTATATGATGGCTTCTCGTAGGAGTTTTGTGGACAGCTTTCTTGACGTAAAGGAGGGCCGCTACAATCCTAAAATGTCACCTTACATACCAAAAGAAAAATGGCGAAAAGGATCCCAGTGGATTGCTTTGGTACGAAGCCATGCAGAAGTCCTTGTGGACGATGATGTTGTTCTTCCAGTGTTCAAAAGTTTTTGCAAGCGTCGCCCGCCTGTGGATGTCAGAAAGGGAAAGCTGAATCTTAAACTTCAGAAGCAGCACAACTGTATCCCAGATGAACACTACGTGCAGACACTGCTTGCGATGAGTGAGCTTGAAGGCGAACTTGAGCGAAGGACATTAACCTATTCGTTGTGGAACAATTCTGCTGCAACTAAAACCGAGAGTAAAGGCTGGCATCCTATGACTTTCAACCACGCAAATGCGGCGCCTAAAAAAATAAAAGAAATAAAGGATGTCAACCACGTATACTATGAGACAGAATACCGGACAGAATGGTGTCGTGTCAATTCAACATACGTTCCTTGTTTCTTATTCGCAAGGAAATTCTCACCGGGAGCTGCCATGCGCCTTCTTAGCGGGGGAGTAGTCGGTCAATTTGATACCTCTGCACTGTTAAATCCGCCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

469

Amino Acids

53.29

Weight (kDa)

9.74

Isoelectric Point (pI)

53.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 153 - 408 2.7e-67 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 595
AccB1I GGYRCC 2 cut(s) 562, 1201
AccBSI CCGCTC 1 cut(s) 493
AccI GTMKAC 3 cut(s) 587, 771, 1242
AccII CGCG 5 cut(s) 13, 280, 329, 461, 595
AclWI GGATC 5 cut(s) 265, 698, 711, 882, 895
AcoI YGGCCR 1 cut(s) 613
AcsI RAATTY 6 cut(s) 118, 184, 218, 417, 538, 1319
AcuI CTGAAG 3 cut(s) 549, 745, 1007
AcyI GRCGYC 2 cut(s) 563, 1202
AdeI CACNNNGTG 1 cut(s) 1060
AfaI GTAC 2 cut(s) 755, 909
AfiI CCNNNNNNNGG 2 cut(s) 351, 988
AgsI TTSAA 8 cut(s) 182, 189, 223, 231, 961, 1091, 1186, 1287
AjiI CACGTC 1 cut(s) 286
AjnI CCWGG 1 cut(s) 565
Alw26I GTCTC 3 cut(s) 17, 603, 1244
AlwI GGATC 5 cut(s) 265, 698, 711, 882, 895
AlwNI CAGNNNCTG 1 cut(s) 1069
AoxI GGCC 3 cut(s) 342, 613, 832
ApeKI GCWGC 6 cut(s) 133, 364, 510, 1030, 1142, 1334
ApoI RAATTY 6 cut(s) 118, 184, 218, 417, 538, 1319
AscI GGCGCGCC 1 cut(s) 278
AspS9I GGNCC 2 cut(s) 342, 832
AsuC2I CCSGG 2 cut(s) 275, 1329
AsuHPI GGTGA 5 cut(s) 255, 322, 433, 846, 1317
BaeI ACNNNNGTAYC 2 cut(s) 1024, 1057
BamHI GGATCC 2 cut(s) 703, 887
BanI GGYRCC 2 cut(s) 562, 1201
BbsI GAAGAC 1 cut(s) 384
BbvI GCAGC 6 cut(s) 145, 376, 522, 1042, 1129, 1321
BccI CCATC 1 cut(s) 778
BceAI ACGGC 5 cut(s) 24, 445, 465, 600, 628
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BciT130I CCWGG 1 cut(s) 567
BciVI GTATCC 1 cut(s) 1052
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 2 cut(s) 275, 1329
BcoDI GTCTC 3 cut(s) 17, 603, 1244
BfaI CTAG 1 cut(s) 473
BfoI RGCGCY 3 cut(s) 536, 566, 1205
BfuI GTATCC 1 cut(s) 1052
BglII AGATCT 1 cut(s) 410
BisI GCNGC 8 cut(s) 134, 365, 511, 835, 1031, 1143, 1200, 1335
BlsI GCNGC 8 cut(s) 135, 366, 512, 836, 1032, 1144, 1201, 1336
Bme1390I CCNGG 3 cut(s) 275, 567, 1329
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 2 cut(s) 342, 832
BmiI GGNNCC 5 cut(s) 356, 564, 705, 889, 1203
BmrFI CCNGG 3 cut(s) 275, 567, 1329
BmrI ACTGGG 1 cut(s) 886
BmsI GCATC 1 cut(s) 1180
BmuI ACTGGG 1 cut(s) 886
BpiI GAAGAC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 1121
BpuMI CCSGG 2 cut(s) 275, 1329
BsaAI YACGTR 2 cut(s) 1060, 1240
BsaHI GRCGYC 2 cut(s) 563, 1202
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 1 cut(s) 427
BsaWI WCCGGW 1 cut(s) 1260
BsaXI ACNNNNNCTCC 4 cut(s) 433, 463, 665, 695
Bsc4I CCNNNNNNNGG 2 cut(s) 351, 988
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 2 cut(s) 892, 953
BseBI CCWGG 1 cut(s) 567
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 3 cut(s) 997, 1171, 1234
BseLI CCNNNNNNNGG 2 cut(s) 351, 988
BseNI ACTGG 2 cut(s) 892, 953
BsePI GCGCGC 1 cut(s) 278
BseRI GAGGAG 8 cut(s) 82, 152, 155, 186, 189, 194, 199, 202
BseX3I CGGCCG 1 cut(s) 613
BseXI GCAGC 6 cut(s) 145, 376, 522, 1042, 1129, 1321
BseYI CCCAGC 2 cut(s) 129, 141
BsgI GTGCAG 2 cut(s) 1082, 1368
Bsh1236I CGCG 5 cut(s) 13, 280, 329, 461, 595
Bsh1285I CGRYCG 1 cut(s) 616
BshFI GGCC 3 cut(s) 344, 615, 834
BshNI GGYRCC 2 cut(s) 562, 1201
BsiEI CGRYCG 1 cut(s) 616
BsiSI CCGG 5 cut(s) 275, 352, 444, 1261, 1328
BslI CCNNNNNNNGG 2 cut(s) 351, 988
BsmAI GTCTC 3 cut(s) 17, 603, 1244
BsmI GAATGC 1 cut(s) 529
BsnI GGCC 3 cut(s) 344, 615, 834
Bso31I GGTCTC 1 cut(s) 603
Bsp1407I TGTACA 1 cut(s) 753
Bsp143I GATC 8 cut(s) 3, 270, 312, 410, 456, 663, 703, 887
BspANI GGCC 3 cut(s) 344, 615, 834
BspFNI CGCG 5 cut(s) 13, 280, 329, 461, 595
BspLI GGNNCC 5 cut(s) 356, 564, 705, 889, 1203
BspPI GGATC 5 cut(s) 265, 698, 711, 882, 895
BspT107I GGYRCC 2 cut(s) 562, 1201
BspTNI GGTCTC 1 cut(s) 603
BsrBI CCGCTC 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 443
BsrGI TGTACA 1 cut(s) 753
BsrI ACTGG 2 cut(s) 892, 953
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 1 cut(s) 427
BssHII GCGCGC 1 cut(s) 278
BssMI GATC 8 cut(s) 3, 270, 312, 410, 456, 663, 703, 887
BssNAI GTATAC 2 cut(s) 772, 1243
BssNI GRCGYC 2 cut(s) 563, 1202
Bst1107I GTATAC 2 cut(s) 772, 1243
Bst2UI CCWGG 1 cut(s) 567
Bst4CI ACNGT 3 cut(s) 776, 1040, 1389
Bst6I CTCTTC 2 cut(s) 173, 388
BstACI GRCGYC 2 cut(s) 563, 1202
BstAPI GCANNNNNTGC 1 cut(s) 1069
BstAUI TGTACA 1 cut(s) 753
BstBAI YACGTR 2 cut(s) 1060, 1240
BstC8I GCNNGC 6 cut(s) 280, 478, 974, 983, 1074, 1169
BstDEI CTNAG 1 cut(s) 1349
BstDSI CCRYGG 1 cut(s) 427
BstF5I GGATG 3 cut(s) 997, 1171, 1234
BstFNI CGCG 5 cut(s) 13, 280, 329, 461, 595
BstH2I RGCGCY 3 cut(s) 536, 566, 1205
BstKTI GATC 8 cut(s) 6, 273, 315, 413, 459, 666, 706, 890
BstMAI GTCTC 3 cut(s) 17, 603, 1244
BstMBI GATC 8 cut(s) 3, 270, 312, 410, 456, 663, 703, 887
BstMCI CGRYCG 1 cut(s) 616
BstMWI GCNNNNNNNGC 9 cut(s) 142, 144, 361, 444, 978, 982, 1069, 1199, 1350
BstNI CCWGG 1 cut(s) 567
BstSCI CCNGG 3 cut(s) 273, 565, 1327
BstUI CGCG 5 cut(s) 13, 280, 329, 461, 595
BstV1I GCAGC 6 cut(s) 145, 376, 522, 1042, 1129, 1321
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 3 cut(s) 410, 703, 887
BstYI RGATCY 3 cut(s) 410, 703, 887
BstZ17I GTATAC 2 cut(s) 772, 1243
BstZI CGGCCG 1 cut(s) 613
BsuI GTATCC 1 cut(s) 1052
BsuRI GGCC 3 cut(s) 344, 615, 834
BtgI CCRYGG 1 cut(s) 427
BtgZI GCGATG 1 cut(s) 1091
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 3 cut(s) 997, 1171, 1234
BtsI GCAGTG 1 cut(s) 1067
BtsIMutI CAGTG 4 cut(s) 899, 960, 1067, 1385
Cac8I GCNNGC 6 cut(s) 280, 478, 974, 983, 1074, 1169
CaiI CAGNNNCTG 1 cut(s) 1069
Cfr10I RCCGGY 1 cut(s) 443
Cfr13I GGNCC 2 cut(s) 342, 832
CseI GACGC 2 cut(s) 601, 965
Csp6I GTAC 2 cut(s) 754, 908
CviAII CATG 3 cut(s) 917, 1339, 1404
CviQI GTAC 2 cut(s) 754, 908
DdeI CTNAG 1 cut(s) 1349
DinI GGCGCC 2 cut(s) 564, 1203
DpnI GATC 8 cut(s) 5, 272, 314, 412, 458, 665, 705, 889
DpnII GATC 8 cut(s) 3, 270, 312, 410, 456, 663, 703, 887
DraIII CACNNNGTG 1 cut(s) 1060
DrdI GACNNNNNNGTC 1 cut(s) 595
DseDI GACNNNNNNGTC 1 cut(s) 595
EaeI YGGCCR 1 cut(s) 613
EagI CGGCCG 1 cut(s) 613
Eam1104I CTCTTC 2 cut(s) 173, 388
EarI CTCTTC 2 cut(s) 173, 388
EciI GGCGGA 1 cut(s) 1386
EclXI CGGCCG 1 cut(s) 613
Eco31I GGTCTC 1 cut(s) 603
Eco52I CGGCCG 1 cut(s) 613
Eco57I CTGAAG 3 cut(s) 549, 745, 1007
EcoRI GAATTC 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 565
EgeI GGCGCC 2 cut(s) 564, 1203
EheI GGCGCC 2 cut(s) 564, 1203
FaeI CATG 3 cut(s) 920, 1342, 1407
FalI AAGNNNNNCTT 2 cut(s) 1083, 1115
FatI CATG 3 cut(s) 916, 1338, 1403
FauI CCCGC 6 cut(s) 431, 485, 498, 715, 990, 1346
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 3 cut(s) 587, 771, 1242
Fnu4HI GCNGC 8 cut(s) 134, 365, 511, 835, 1031, 1143, 1200, 1335
FokI GGATG 3 cut(s) 1004, 1158, 1241
Fsp4HI GCNGC 8 cut(s) 134, 365, 511, 835, 1031, 1143, 1200, 1335
FspBI CTAG 1 cut(s) 473
GluI GCNGC 8 cut(s) 134, 365, 511, 835, 1031, 1143, 1200, 1335
GsaI CCCAGC 2 cut(s) 133, 145
HaeII RGCGCY 3 cut(s) 536, 566, 1205
HaeIII GGCC 3 cut(s) 344, 615, 834
HapII CCGG 5 cut(s) 275, 352, 444, 1261, 1328
HgaI GACGC 2 cut(s) 601, 965
Hin1I GRCGYC 2 cut(s) 563, 1202
Hin1II CATG 3 cut(s) 920, 1342, 1407
HincII GTYRAC 2 cut(s) 588, 1234
HindII GTYRAC 2 cut(s) 588, 1234
HindIII AAGCTT 1 cut(s) 436
HinfI GANTC 6 cut(s) 25, 57, 70, 584, 653, 1012
HpaII CCGG 5 cut(s) 275, 352, 444, 1261, 1328
HphI GGTGA 5 cut(s) 255, 322, 433, 846, 1317
Hpy166II GTNNAC 7 cut(s) 74, 588, 772, 808, 934, 1234, 1243
Hpy188I TCNGA 7 cut(s) 22, 96, 217, 453, 736, 998, 1026
Hpy188III TCNNGA 3 cut(s) 414, 520, 818
Hpy8I GTNNAC 7 cut(s) 74, 588, 772, 808, 934, 1234, 1243
Hpy99I CGWCG 4 cut(s) 329, 592, 595, 981
HpyAV CCTTC 5 cut(s) 87, 237, 1085, 1101, 1355
HpyCH4III ACNGT 3 cut(s) 776, 1040, 1389
HpyCH4IV ACGT 5 cut(s) 285, 822, 1059, 1239, 1293
HpyCH4V TGCA 6 cut(s) 689, 920, 972, 1063, 1145, 1385
HpyF10VI GCNNNNNNNGC 9 cut(s) 142, 144, 361, 444, 978, 982, 1069, 1199, 1350
HpyF3I CTNAG 1 cut(s) 1349
HpySE526I ACGT 5 cut(s) 285, 822, 1059, 1239, 1293
Hsp92I GRCGYC 2 cut(s) 563, 1202
Hsp92II CATG 3 cut(s) 920, 1342, 1407
KasI GGCGCC 2 cut(s) 562, 1201
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 8 cut(s) 3, 270, 312, 410, 456, 663, 703, 887
LmnI GCTCC 3 cut(s) 199, 360, 1331
Lsp1109I GCAGC 6 cut(s) 145, 376, 522, 1042, 1129, 1321
LweI GCATC 1 cut(s) 1180
MaeI CTAG 1 cut(s) 473
MaeII ACGT 5 cut(s) 285, 822, 1059, 1239, 1293
MaeIII GTNAC 2 cut(s) 261, 852
MalI GATC 8 cut(s) 5, 272, 314, 412, 458, 665, 705, 889
MbiI CCGCTC 1 cut(s) 493
MboI GATC 8 cut(s) 3, 270, 312, 410, 456, 663, 703, 887
MboII GAAGA 7 cut(s) 160, 268, 375, 384, 466, 508, 941
MfeI CAATTG 1 cut(s) 620
MflI RGATCY 3 cut(s) 410, 703, 887
Mly113I GGCGCC 2 cut(s) 563, 1202
MlyI GAGTC 4 cut(s) 19, 66, 79, 593
MseI TTAA 3 cut(s) 1017, 1115, 1391
MslI CAYNNNNRTG 2 cut(s) 638, 1194
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 5 cut(s) 275, 352, 444, 1261, 1328
MspR9I CCNGG 3 cut(s) 275, 567, 1329
MunI CAATTG 1 cut(s) 620
Mva1269I GAATGC 1 cut(s) 529
MvaI CCWGG 1 cut(s) 567
MvnI CGCG 5 cut(s) 13, 280, 329, 461, 595
MwoI GCNNNNNNNGC 9 cut(s) 142, 144, 361, 444, 978, 982, 1069, 1199, 1350
NarI GGCGCC 2 cut(s) 563, 1202
NciI CCSGG 2 cut(s) 275, 1329
NdeII GATC 8 cut(s) 3, 270, 312, 410, 456, 663, 703, 887
NlaIII CATG 3 cut(s) 920, 1342, 1407
NlaIV GGNNCC 5 cut(s) 356, 564, 705, 889, 1203
NmeAIII GCCGAG 1 cut(s) 539
NmuCI GTSAC 2 cut(s) 261, 852
PalAI GGCGCGCC 1 cut(s) 278
PauI GCGCGC 1 cut(s) 278
PcsI WCGNNNNNNNCGW 3 cut(s) 248, 321, 333
PctI GAATGC 1 cut(s) 529
PfeI GAWTC 2 cut(s) 653, 1012
PflFI GACNNNGTC 2 cut(s) 56, 595
PkrI GCNGC 8 cut(s) 135, 366, 512, 836, 1032, 1144, 1201, 1336
PleI GAGTC 4 cut(s) 19, 65, 78, 592
PluTI GGCGCC 2 cut(s) 566, 1205
PpsI GAGTC 4 cut(s) 19, 65, 78, 592
Ppu21I YACGTR 2 cut(s) 1060, 1240
Psp6I CCWGG 1 cut(s) 565
PspFI CCCAGC 2 cut(s) 129, 141
PspGI CCWGG 1 cut(s) 565
PspN4I GGNNCC 5 cut(s) 356, 564, 705, 889, 1203
PspPI GGNCC 2 cut(s) 342, 832
PstNI CAGNNNCTG 1 cut(s) 1069
PsuI RGATCY 3 cut(s) 410, 703, 887
PsyI GACNNNGTC 2 cut(s) 56, 595
PteI GCGCGC 1 cut(s) 278
RsaI GTAC 2 cut(s) 755, 909
RsaNI GTAC 2 cut(s) 754, 908
RseI CAYNNNNRTG 2 cut(s) 638, 1194
SalI GTCGAC 1 cut(s) 586
SaqAI TTAA 3 cut(s) 1017, 1115, 1391
SatI GCNGC 8 cut(s) 134, 365, 511, 835, 1031, 1143, 1200, 1335
Sau3AI GATC 8 cut(s) 3, 270, 312, 410, 456, 663, 703, 887
Sau96I GGNCC 2 cut(s) 342, 832
SchI GAGTC 4 cut(s) 19, 66, 79, 593
ScrFI CCNGG 3 cut(s) 275, 567, 1329
SfaNI GCATC 1 cut(s) 1180
SfoI GGCGCC 2 cut(s) 564, 1203
SgsI GGCGCGCC 1 cut(s) 278
SmiMI CAYNNNNRTG 2 cut(s) 638, 1194
SmlI CTYRAG 1 cut(s) 1100
SmoI CTYRAG 1 cut(s) 1100
SspDI GGCGCC 2 cut(s) 562, 1201
SspMI CTAG 1 cut(s) 473
StyD4I CCNGG 3 cut(s) 273, 565, 1327
TaaI ACNGT 3 cut(s) 776, 1040, 1389
TaiI ACGT 5 cut(s) 288, 825, 1062, 1242, 1296
TaqI TCGA 8 cut(s) 415, 495, 519, 545, 579, 587, 656, 666
TaqII GACCGA 1 cut(s) 1355
TatI WGTACW 1 cut(s) 753
TauI GCSGC 2 cut(s) 837, 1202
TfiI GAWTC 2 cut(s) 653, 1012
Tru1I TTAA 3 cut(s) 1017, 1115, 1391
Tru9I TTAA 3 cut(s) 1017, 1115, 1391
TscAI CASTG 4 cut(s) 899, 960, 1074, 1392
TseFI GTSAC 2 cut(s) 261, 852
TseI GCWGC 6 cut(s) 133, 364, 510, 1030, 1142, 1334
Tsp45I GTSAC 2 cut(s) 261, 852
TspDTI ATGAA 2 cut(s) 269, 1065
TspGWI ACGGA 2 cut(s) 313, 378
TspRI CASTG 4 cut(s) 899, 960, 1074, 1392
Tth111I GACNNNGTC 2 cut(s) 56, 595
XapI RAATTY 6 cut(s) 118, 184, 218, 417, 538, 1319
XcmI CCANNNNNNNNNTGG 1 cut(s) 873
XmiI GTMKAC 3 cut(s) 587, 771, 1242
XspI CTAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.