Rh2AG536500

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002602
Physical Location & Seq
Forward (+)
76450796 .. 76453814
3019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG536500.1

Sequence Viewer

Length: 912 bp
ATGGCAAAGAGAAGAGCTTCATCACCGATGCGCCAACTTTCGAGGTTGAGGTCAAGACTCATCATCATCCTCTGTGTCTCGCTATGTGTACTGGCTTTACTTAGAGTAGTATTCCATTCTGAGTCCAACCTCCAGTCCTCATTCCCCACCTCTCGAAGAAGGTCTCGTGCTTTTATTGATATTCAGTTTGTTGGCCCTCCAAAGGTTGCTTTTCTCTTCCTGGTTCGGCGGGATCTCCCTCTTGATTTTCTCTGGGGCACCTTCTTCAAGAATGGTGATAGAGCAAAATTCTCCATTTACGTTCATTCAGAGCCTGGTTTTGTGTTTGATGGATCAACCACAAGGTCTGCATTCTTCTTTGGTCGACAGTTAAACAACAGCATTCAGGTAGTATGGGGAGAATCAAGTATGATCGAAGCAGAGCGGTTACTATTTGAAGAAGCTCTTTATGATCCAGCCAATCAAAGATTTGTCCTTCTCTCAGATAGCTGTGTACCTCTCTACAACTTCAGTTACATTTACAACTATGTAATGTCTTCTCCAAAAAGCTTCGTTGACAGCTTTATTGATGTTAAAGGACATCGTTATGATCCAAAGATGTCACCTACTATACCTAAGGAGAGATGGCGAAAAGGATCCCAGTGGATAACATTGGCTCGAAGACATGCTGAAATTGTTGTGGATGACGACACTGTTTTCCCCGTCTTCAGGGAATTCTGCAAGCGATGGCCGCCTACACCCCTGAGTTTTAGAAGGCGACTACTTGCAATCAATTGGACTTTCAAGCTTCCATTGTTTACATTTCAGAGGCATCGCAATTGTATCCCCGATGAACATTATGTGCAGACACTACTTGCAGTAAGCAGAATGCTTCCTATGCTTTTCAGTCTTGATAGTCATAGTATATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

303

Amino Acids

35.49

Weight (kDa)

10.05

Isoelectric Point (pI)

65.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 69 - 288 2.2e-59 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 257
AccBSI CCGCTC 1 cut(s) 424
AccI GTMKAC 1 cut(s) 364
AciI CCGC 3 cut(s) 229, 424, 731
AclWI GGATC 6 cut(s) 240, 340, 446, 584, 630, 643
AcoI YGGCCR 1 cut(s) 728
AcsI RAATTY 2 cut(s) 287, 713
AcuI CTGAAG 2 cut(s) 493, 691
AfaI GTAC 2 cut(s) 90, 495
AfiI CCNNNNNNNGG 2 cut(s) 202, 708
AgsI TTSAA 3 cut(s) 268, 437, 784
AjnI CCWGG 2 cut(s) 219, 313
AluBI AGCT 6 cut(s) 17, 443, 489, 549, 561, 787
AluI AGCT 6 cut(s) 17, 443, 489, 549, 561, 787
Alw26I GTCTC 2 cut(s) 82, 168
AlwI GGATC 6 cut(s) 240, 340, 446, 584, 630, 643
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 2 cut(s) 193, 728
ApoI RAATTY 2 cut(s) 287, 713
Asp700I GAANNNNTTC 1 cut(s) 16
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 194
AsuHPI GGTGA 3 cut(s) 15, 287, 594
AxyI CCTNAGG 1 cut(s) 615
BaeGI GKGCMC 1 cut(s) 260
BamHI GGATCC 1 cut(s) 635
BanI GGYRCC 1 cut(s) 257
BauI CACGAG 1 cut(s) 165
BbsI GAAGAC 3 cut(s) 528, 667, 697
BccI CCATC 3 cut(s) 323, 618, 720
BciT130I CCWGG 2 cut(s) 221, 315
BciVI GTATCC 1 cut(s) 833
BcoDI GTCTC 2 cut(s) 82, 168
BfuI GTATCC 1 cut(s) 833
BisI GCNGC 1 cut(s) 731
BlsI GCNGC 1 cut(s) 732
Bme1390I CCNGG 2 cut(s) 221, 315
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 2 cut(s) 259, 637
BmrFI CCNGG 2 cut(s) 221, 315
BmrI ACTGGG 1 cut(s) 634
BmsI GCATC 2 cut(s) 18, 820
BmuI ACTGGG 1 cut(s) 634
BpiI GAAGAC 3 cut(s) 528, 667, 697
BpmI CTGGAG 1 cut(s) 116
BsaI GGTCTC 1 cut(s) 168
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 708
Bse1I ACTGG 3 cut(s) 96, 133, 640
Bse21I CCTNAGG 1 cut(s) 615
BseBI CCWGG 2 cut(s) 221, 315
BseGI GGATG 2 cut(s) 66, 688
BseLI CCNNNNNNNGG 2 cut(s) 202, 708
BseMII CTCAG 3 cut(s) 111, 495, 734
BseNI ACTGG 3 cut(s) 96, 133, 640
BseSI GKGCMC 1 cut(s) 260
BsgI GTGCAG 1 cut(s) 863
BshFI GGCC 2 cut(s) 195, 730
BshNI GGYRCC 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 202, 708
BsmAI GTCTC 2 cut(s) 82, 168
BsmI GAATGC 3 cut(s) 350, 381, 873
BsnI GGCC 2 cut(s) 195, 730
Bso31I GGTCTC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 260
Bsp143I GATC 6 cut(s) 232, 332, 411, 451, 589, 635
BspACI CCGC 3 cut(s) 229, 424, 731
BspANI GGCC 2 cut(s) 195, 730
BspCNI CTCAG 3 cut(s) 112, 494, 735
BspHI TCATGA 1 cut(s) 908
BspLI GGNNCC 2 cut(s) 259, 637
BspPI GGATC 6 cut(s) 240, 340, 446, 584, 630, 643
BspQI GCTCTTC 1 cut(s) 7
BspT107I GGYRCC 1 cut(s) 257
BspTNI GGTCTC 1 cut(s) 168
BsrBI CCGCTC 1 cut(s) 424
BsrI ACTGG 3 cut(s) 96, 133, 640
BssMI GATC 6 cut(s) 232, 332, 411, 451, 589, 635
BssSI CACGAG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 221, 315
Bst4CI ACNGT 2 cut(s) 369, 694
Bst6I CTCTTC 2 cut(s) 7, 221
BstC8I GCNNGC 1 cut(s) 722
BstDEI CTNAG 5 cut(s) 101, 120, 481, 615, 743
BstF5I GGATG 2 cut(s) 66, 688
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 6 cut(s) 235, 335, 414, 454, 592, 638
BstMAI GTCTC 2 cut(s) 82, 168
BstMBI GATC 6 cut(s) 232, 332, 411, 451, 589, 635
BstMWI GCNNNNNNNGC 2 cut(s) 730, 877
BstNI CCWGG 2 cut(s) 221, 315
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 2 cut(s) 219, 313
BstSLI GKGCMC 1 cut(s) 260
BstV2I GAAGAC 3 cut(s) 528, 667, 697
BstX2I RGATCY 2 cut(s) 232, 635
BstYI RGATCY 2 cut(s) 232, 635
Bsu36I CCTNAGG 1 cut(s) 615
BsuI GTATCC 1 cut(s) 833
BsuRI GGCC 2 cut(s) 195, 730
BtgZI GCGATG 2 cut(s) 739, 797
BtsCI GGATG 2 cut(s) 66, 688
BtsIMutI CAGTG 2 cut(s) 647, 690
Cac8I GCNNGC 1 cut(s) 722
CaiI CAGNNNCTG 1 cut(s) 314
CciI TCATGA 1 cut(s) 908
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 1 cut(s) 194
Csp6I GTAC 2 cut(s) 89, 494
CviAII CATG 2 cut(s) 665, 909
CviQI GTAC 2 cut(s) 89, 494
DdeI CTNAG 5 cut(s) 101, 120, 481, 615, 743
DpnI GATC 6 cut(s) 234, 334, 413, 453, 591, 637
DpnII GATC 6 cut(s) 232, 332, 411, 451, 589, 635
EaeI YGGCCR 1 cut(s) 728
Eam1104I CTCTTC 2 cut(s) 7, 221
EarI CTCTTC 2 cut(s) 7, 221
Eco31I GGTCTC 1 cut(s) 168
Eco57I CTGAAG 2 cut(s) 493, 691
Eco81I CCTNAGG 1 cut(s) 615
EcoRI GAATTC 1 cut(s) 713
EcoRII CCWGG 2 cut(s) 219, 313
FaeI CATG 2 cut(s) 668, 912
FalI AAGNNNNNCTT 2 cut(s) 429, 461
FatI CATG 2 cut(s) 664, 908
FauI CCCGC 1 cut(s) 222
FblI GTMKAC 1 cut(s) 364
Fnu4HI GCNGC 1 cut(s) 731
FokI GGATG 2 cut(s) 53, 695
Fsp4HI GCNGC 1 cut(s) 731
GlaI GCGC 1 cut(s) 32
GluI GCNGC 1 cut(s) 731
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 2 cut(s) 195, 730
HhaI GCGC 1 cut(s) 33
Hin1II CATG 2 cut(s) 668, 912
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 2 cut(s) 365, 556
HindII GTYRAC 2 cut(s) 365, 556
HindIII AAGCTT 2 cut(s) 547, 785
HinfI GANTC 3 cut(s) 57, 122, 401
HphI GGTGA 3 cut(s) 15, 287, 594
Hpy166II GTNNAC 5 cut(s) 89, 365, 494, 556, 798
Hpy188I TCNGA 4 cut(s) 121, 310, 484, 807
Hpy188III TCNNGA 6 cut(s) 54, 153, 242, 268, 890, 909
Hpy8I GTNNAC 5 cut(s) 89, 365, 494, 556, 798
HpyAV CCTTC 4 cut(s) 153, 271, 485, 747
HpyCH4III ACNGT 2 cut(s) 369, 694
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 5 cut(s) 350, 720, 767, 844, 857
HpyF10VI GCNNNNNNNGC 2 cut(s) 730, 877
HpyF3I CTNAG 5 cut(s) 101, 120, 481, 615, 743
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 2 cut(s) 668, 912
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 6 cut(s) 232, 332, 411, 451, 589, 635
LguI GCTCTTC 1 cut(s) 7
LweI GCATC 2 cut(s) 18, 820
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 3 cut(s) 426, 512, 600
MalI GATC 6 cut(s) 234, 334, 413, 453, 591, 637
MbiI CCGCTC 1 cut(s) 424
MboI GATC 6 cut(s) 232, 332, 411, 451, 589, 635
MboII GAAGA 9 cut(s) 24, 168, 208, 256, 346, 449, 528, 672, 697
MfeI CAATTG 2 cut(s) 772, 817
MflI RGATCY 2 cut(s) 232, 635
MhlI GDGCHC 1 cut(s) 260
MluCI AATT 5 cut(s) 287, 672, 713, 772, 817
MlyI GAGTC 2 cut(s) 51, 131
MmeI TCCRAC 1 cut(s) 150
MroXI GAANNNNTTC 1 cut(s) 16
MseI TTAA 2 cut(s) 371, 573
MslI CAYNNNNRTG 1 cut(s) 585
MspR9I CCNGG 2 cut(s) 221, 315
MunI CAATTG 2 cut(s) 772, 817
Mva1269I GAATGC 3 cut(s) 350, 381, 873
MvaI CCWGG 2 cut(s) 221, 315
MwoI GCNNNNNNNGC 2 cut(s) 730, 877
NdeII GATC 6 cut(s) 232, 332, 411, 451, 589, 635
NlaIII CATG 2 cut(s) 668, 912
NlaIV GGNNCC 2 cut(s) 259, 637
NmuCI GTSAC 1 cut(s) 600
NspI RCATGY 1 cut(s) 668
PagI TCATGA 1 cut(s) 908
PciSI GCTCTTC 1 cut(s) 7
PctI GAATGC 3 cut(s) 350, 381, 873
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 401
PkrI GCNGC 1 cut(s) 732
PleI GAGTC 2 cut(s) 51, 130
PpsI GAGTC 2 cut(s) 51, 130
Psp6I CCWGG 2 cut(s) 219, 313
PspGI CCWGG 2 cut(s) 219, 313
PspN4I GGNNCC 2 cut(s) 259, 637
PspPI GGNCC 1 cut(s) 194
PstNI CAGNNNCTG 1 cut(s) 314
PsuI RGATCY 2 cut(s) 232, 635
RsaI GTAC 2 cut(s) 90, 495
RsaNI GTAC 2 cut(s) 89, 494
RseI CAYNNNNRTG 1 cut(s) 585
SalI GTCGAC 1 cut(s) 363
SapI GCTCTTC 1 cut(s) 7
SaqAI TTAA 2 cut(s) 371, 573
SatI GCNGC 1 cut(s) 731
Sau3AI GATC 6 cut(s) 232, 332, 411, 451, 589, 635
Sau96I GGNCC 1 cut(s) 194
SchI GAGTC 2 cut(s) 51, 131
ScrFI CCNGG 2 cut(s) 221, 315
SduI GDGCHC 1 cut(s) 260
SfaNI GCATC 2 cut(s) 18, 820
SmiMI CAYNNNNRTG 1 cut(s) 585
Sse9I AATT 5 cut(s) 287, 672, 713, 772, 817
SsiI CCGC 3 cut(s) 229, 424, 731
StyD4I CCNGG 2 cut(s) 219, 313
TaaI ACNGT 2 cut(s) 369, 694
TaiI ACGT 1 cut(s) 303
TaqI TCGA 5 cut(s) 41, 154, 364, 414, 658
TasI AATT 5 cut(s) 287, 672, 713, 772, 817
TatI WGTACW 1 cut(s) 88
TauI GCSGC 1 cut(s) 733
TfiI GAWTC 1 cut(s) 401
Tru1I TTAA 2 cut(s) 371, 573
Tru9I TTAA 2 cut(s) 371, 573
TscAI CASTG 2 cut(s) 647, 697
TseFI GTSAC 1 cut(s) 600
Tsp45I GTSAC 1 cut(s) 600
TspDTI ATGAA 3 cut(s) 9, 293, 846
TspRI CASTG 2 cut(s) 647, 697
XapI RAATTY 2 cut(s) 287, 713
XceI RCATGY 1 cut(s) 668
XmiI GTMKAC 1 cut(s) 364
XmnI GAANNNNTTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.