Rroxscaffold_1G00073460

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002602
Physical Location & Seq
Reverse (-)
94311646 .. 94315680
4035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073460.1

Sequence Viewer

Length: 1281 bp
ATGGTGACGATGACGAAGAAGACGTCGCCGCCGCCAATCACGGCGCGCCACGTGATCAGGTCCAGCTGGAAGCTTCTGATCGTCTTCTCCGTCTTGCTCTGCGTTTTGGCTCTGTACAGGTTTCAGTCTCAGCAGCCAGACCTTTACTCTCCTTCTTCATTATCTCGCGCGAGATCTCGTATTTCTCGCCACAGCGTCAGCTTCGCCGGCCCCGCCAAGGTCGCCTTCCTATTTCTCGCACGACGTGACCTCCCGCTCGATTTTCTGTGGGAGAGCTTCTTCGAGGTTCGAGGTCTAGCTCTTTTTTGTGTTTTTGCTTTGAATTTCGGGAATTTCAATTGGTGGTTTGGTTGTCGGAGACGGAGAGTGACGGAGAATTTTGTTCGGTTTTGGCAGAATGCCGGTGGCGCTTTGAATTTTTCGATTTATATTCACTCCGCGCCTGGTTTTGTGTTCGATGAGTCCACCACGAGGTCTCGCTTCTTTCACGGCCGTCAGTTACCGAATAGCATACAGGTGGGATGGGGAGAATCGAGCATGATTGAAGCGGAGAGGTTGTTGTTTGCAACAGCTCTTGAGGATCCAGCAAATCAAAGATTTGTTCTCCTCTCTGACAGTTGCGTTCCTTTGTACAACTTCAGCTTCATATACAATTACCTGATGGCTTCTCCTAGGAGTATTGTGGACAGCTTCCTGGATGTGAAGGAGGGCCGCTACAACCCAAAAATGTCACCCATAATACCAAAAGCCAAATGGCGGAAAGGATCACAGTGGATTGCTTTGATTCGGACACACGCTGAAGTCCTTGTGGATGATGAAGTTATACTACCAGTTTTCAAAAAGTTTTGCAGACGTCGTCCACCTGTTGATGTCAGAAAGGGGAAGCTGAATCTTAAACTTCAGAAACAACACAACTGTATCCCAGACGAACACTATGTGCAGACACTGCTTGCGATGTGTGAGCTTGACGGCGAACTTGAGCGAAGGACATTAACCTATACATTGTGGAGCAACTCTCCAAATAAAACAGAGAGTAAAGGCTGGCATCCTATGACTTTCAACCATGCCAATGCGGCCCCTCAAAAGATTAGGGAAATAAAGGGGATCAACCATGTATACTATGAGACAGAATTTCGAACAGAATGGTGTCGTATAAATTCTACTGTTGTTCCCTGTTTTTTATTCGCAAGGAAGTTCTCCAAGGGTGCTGCCATGCGCCTTCTGAGCGAGGGAGTTGTTGGTCACTTTGACACAGCTGCATTGTTAAAACCATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

426

Amino Acids

49.15

Weight (kDa)

9.81

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 139 - 366 2.9e-60 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 26, 856
AccBSI CCGCTC 1 cut(s) 256
AccI GTMKAC 1 cut(s) 1116
AccII CGCG 4 cut(s) 46, 168, 170, 440
AciI CCGC 9 cut(s) 29, 32, 213, 254, 438, 548, 712, 757, 1073
AclWI GGATC 4 cut(s) 575, 588, 772, 1112
AcoI YGGCCR 1 cut(s) 490
AcsI RAATTY 6 cut(s) 322, 331, 376, 415, 1130, 1156
AcuI CTGAAG 3 cut(s) 622, 819, 884
AcvI CACGTG 1 cut(s) 52
AcyI GRCGYC 2 cut(s) 23, 853
AdeI CACNNNGTG 2 cut(s) 245, 937
AfaI GTAC 2 cut(s) 116, 632
AfiI CCNNNNNNNGG 3 cut(s) 217, 471, 756
AgsI TTSAA 6 cut(s) 322, 337, 415, 545, 838, 1060
AjiI CACGTC 1 cut(s) 245
AjnI CCWGG 2 cut(s) 442, 693
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
Alw26I GTCTC 4 cut(s) 132, 352, 480, 1118
AlwI GGATC 4 cut(s) 575, 588, 772, 1112
AlwNI CAGNNNCTG 1 cut(s) 946
AoxI GGCC 4 cut(s) 208, 490, 709, 1074
ApeKI GCWGC 3 cut(s) 133, 1208, 1256
ApoI RAATTY 6 cut(s) 322, 331, 376, 415, 1130, 1156
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AscI GGCGCGCC 1 cut(s) 44
AspA2I CCTAGG 1 cut(s) 671
AspLEI GCGC 6 cut(s) 46, 48, 170, 410, 442, 1218
AspS9I GGNCC 4 cut(s) 60, 209, 709, 1075
AsuHPI GGTGA 3 cut(s) 16, 723, 1266
AsuII TTCGAA 1 cut(s) 1135
AvaII GGWCC 1 cut(s) 60
AvrII CCTAGG 1 cut(s) 671
BaeI ACNNNNGTAYC 2 cut(s) 901, 934
BamHI GGATCC 1 cut(s) 580
BarI GAAGNNNNNNTAC 2 cut(s) 810, 842
BauI CACGAG 1 cut(s) 469
BbrPI CACGTG 1 cut(s) 52
BbsI GAAGAC 2 cut(s) 26, 76
BbvI GCAGC 3 cut(s) 145, 1195, 1243
BccI CCATC 3 cut(s) 516, 655, 1279
BceAI ACGGC 4 cut(s) 57, 477, 505, 985
BciT130I CCWGG 2 cut(s) 444, 695
BciVI GTATCC 1 cut(s) 929
BclI TGATCA 1 cut(s) 54
BcoDI GTCTC 4 cut(s) 132, 352, 480, 1118
BfaI CTAG 2 cut(s) 296, 672
BfoI RGCGCY 1 cut(s) 411
BfuI GTATCC 1 cut(s) 929
BglI GCCNNNNNGGC 1 cut(s) 1073
BglII AGATCT 1 cut(s) 173
BisI GCNGC 7 cut(s) 29, 32, 134, 712, 1074, 1209, 1257
BlnI CCTAGG 1 cut(s) 671
BlsI GCNGC 7 cut(s) 30, 33, 135, 713, 1075, 1210, 1258
Bme1390I CCNGG 2 cut(s) 444, 695
Bme18I GGWCC 1 cut(s) 60
BmgBI CACGTC 1 cut(s) 245
BmgT120I GGNCC 4 cut(s) 60, 209, 709, 1075
BmiI GGNNCC 3 cut(s) 211, 582, 1077
BmrFI CCNGG 2 cut(s) 444, 695
BmsI GCATC 1 cut(s) 1054
BpiI GAAGAC 2 cut(s) 26, 76
BplI GAGNNNNNCTC 2 cut(s) 1000, 1032
Bpu14I TTCGAA 1 cut(s) 1135
BpuEI CTTGAG 2 cut(s) 596, 998
BsaAI YACGTR 1 cut(s) 52
BsaHI GRCGYC 2 cut(s) 23, 853
BsaI GGTCTC 1 cut(s) 480
BsaJI CCNNGG 3 cut(s) 216, 671, 1200
BsaXI ACNNNNNCTCC 4 cut(s) 234, 264, 542, 572
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 471, 756
Bse118I RCCGGY 2 cut(s) 206, 401
Bse1I ACTGG 1 cut(s) 830
BseBI CCWGG 2 cut(s) 444, 695
BseDI CCNNGG 3 cut(s) 216, 671, 1200
BseGI GGATG 4 cut(s) 527, 703, 817, 1045
BseLI CCNNNNNNNGG 3 cut(s) 217, 471, 756
BseMII CTCAG 2 cut(s) 143, 1214
BseNI ACTGG 1 cut(s) 830
BsePI GCGCGC 1 cut(s) 44
BseRI GAGGAG 1 cut(s) 596
BseX3I CGGCCG 1 cut(s) 490
BseXI GCAGC 3 cut(s) 145, 1195, 1243
BsgI GTGCAG 1 cut(s) 959
Bsh1236I CGCG 4 cut(s) 46, 168, 170, 440
Bsh1285I CGRYCG 1 cut(s) 493
BshFI GGCC 4 cut(s) 210, 492, 711, 1076
BsiEI CGRYCG 1 cut(s) 493
BsiSI CCGG 2 cut(s) 207, 402
BslI CCNNNNNNNGG 3 cut(s) 217, 471, 756
BsmAI GTCTC 4 cut(s) 132, 352, 480, 1118
BsmBI CGTCTC 1 cut(s) 352
BsmI GAATGC 1 cut(s) 403
BsnI GGCC 4 cut(s) 210, 492, 711, 1076
Bso31I GGTCTC 1 cut(s) 480
Bsp119I TTCGAA 1 cut(s) 1135
Bsp1407I TGTACA 2 cut(s) 114, 630
Bsp143I GATC 6 cut(s) 54, 78, 173, 580, 764, 1104
BspACI CCGC 9 cut(s) 29, 32, 213, 254, 438, 548, 712, 757, 1073
BspANI GGCC 4 cut(s) 210, 492, 711, 1076
BspCNI CTCAG 2 cut(s) 142, 1215
BspFNI CGCG 4 cut(s) 46, 168, 170, 440
BspLI GGNNCC 3 cut(s) 211, 582, 1077
BspPI GGATC 4 cut(s) 575, 588, 772, 1112
BspT104I TTCGAA 1 cut(s) 1135
BspTNI GGTCTC 1 cut(s) 480
BsrBI CCGCTC 1 cut(s) 256
BsrFI RCCGGY 2 cut(s) 206, 401
BsrGI TGTACA 2 cut(s) 114, 630
BsrI ACTGG 1 cut(s) 830
BssAI RCCGGY 2 cut(s) 206, 401
BssECI CCNNGG 3 cut(s) 216, 671, 1200
BssHII GCGCGC 1 cut(s) 44
BssMI GATC 6 cut(s) 54, 78, 173, 580, 764, 1104
BssNAI GTATAC 1 cut(s) 1117
BssNI GRCGYC 2 cut(s) 23, 853
BssSI CACGAG 1 cut(s) 469
BssT1I CCWWGG 3 cut(s) 216, 671, 1200
Bst1107I GTATAC 1 cut(s) 1117
Bst2BI CACGAG 1 cut(s) 469
Bst2UI CCWGG 2 cut(s) 444, 695
Bst4CI ACNGT 4 cut(s) 617, 771, 917, 1165
BstACI GRCGYC 2 cut(s) 23, 853
BstAPI GCANNNNNTGC 1 cut(s) 946
BstAUI TGTACA 2 cut(s) 114, 630
BstBAI YACGTR 1 cut(s) 52
BstBI TTCGAA 1 cut(s) 1135
BstC8I GCNNGC 4 cut(s) 46, 208, 951, 1043
BstDEI CTNAG 2 cut(s) 129, 1223
BstF5I GGATG 4 cut(s) 527, 703, 817, 1045
BstFNI CGCG 4 cut(s) 46, 168, 170, 440
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 6 cut(s) 46, 48, 170, 410, 442, 1218
BstKTI GATC 6 cut(s) 57, 81, 176, 583, 767, 1107
BstMAI GTCTC 4 cut(s) 132, 352, 480, 1118
BstMBI GATC 6 cut(s) 54, 78, 173, 580, 764, 1104
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 7 cut(s) 207, 212, 221, 407, 946, 1073, 1224
BstNI CCWGG 2 cut(s) 444, 695
BstSCI CCNGG 2 cut(s) 442, 693
BstUI CGCG 4 cut(s) 46, 168, 170, 440
BstV1I GCAGC 3 cut(s) 145, 1195, 1243
BstV2I GAAGAC 2 cut(s) 26, 76
BstX2I RGATCY 2 cut(s) 173, 580
BstYI RGATCY 2 cut(s) 173, 580
BstZ17I GTATAC 1 cut(s) 1117
BstZI CGGCCG 1 cut(s) 490
BsuI GTATCC 1 cut(s) 929
BsuRI GGCC 4 cut(s) 210, 492, 711, 1076
BtgZI GCGATG 1 cut(s) 968
BtrI CACGTC 1 cut(s) 245
BtsCI GGATG 4 cut(s) 527, 703, 817, 1045
BtsI GCAGTG 1 cut(s) 944
BtsIMutI CAGTG 2 cut(s) 776, 944
Cac8I GCNNGC 4 cut(s) 46, 208, 951, 1043
CaiI CAGNNNCTG 1 cut(s) 946
CfoI GCGC 6 cut(s) 46, 48, 170, 410, 442, 1218
Cfr10I RCCGGY 2 cut(s) 206, 401
Cfr13I GGNCC 4 cut(s) 60, 209, 709, 1075
CseI GACGC 1 cut(s) 184
Csp6I GTAC 2 cut(s) 115, 631
CviAII CATG 5 cut(s) 538, 1064, 1112, 1213, 1278
CviQI GTAC 2 cut(s) 115, 631
DdeI CTNAG 2 cut(s) 129, 1223
DpnI GATC 6 cut(s) 56, 80, 175, 582, 766, 1106
DpnII GATC 6 cut(s) 54, 78, 173, 580, 764, 1104
DraIII CACNNNGTG 2 cut(s) 245, 937
EaeI YGGCCR 1 cut(s) 490
EagI CGGCCG 1 cut(s) 490
EciI GGCGGA 1 cut(s) 772
EclXI CGGCCG 1 cut(s) 490
Eco130I CCWWGG 3 cut(s) 216, 671, 1200
Eco31I GGTCTC 1 cut(s) 480
Eco47I GGWCC 1 cut(s) 60
Eco52I CGGCCG 1 cut(s) 490
Eco57I CTGAAG 3 cut(s) 622, 819, 884
Eco72I CACGTG 1 cut(s) 52
EcoRII CCWGG 2 cut(s) 442, 693
EcoT14I CCWWGG 3 cut(s) 216, 671, 1200
ErhI CCWWGG 3 cut(s) 216, 671, 1200
Esp3I CGTCTC 1 cut(s) 352
FaeI CATG 5 cut(s) 541, 1067, 1115, 1216, 1281
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FatI CATG 5 cut(s) 537, 1063, 1111, 1212, 1277
FauI CCCGC 2 cut(s) 220, 261
FbaI TGATCA 1 cut(s) 54
FblI GTMKAC 1 cut(s) 1116
Fnu4HI GCNGC 7 cut(s) 29, 32, 134, 712, 1074, 1209, 1257
FokI GGATG 4 cut(s) 534, 710, 824, 1032
Fsp4HI GCNGC 7 cut(s) 29, 32, 134, 712, 1074, 1209, 1257
FspBI CTAG 2 cut(s) 296, 672
GlaI GCGC 6 cut(s) 45, 47, 169, 409, 441, 1217
GluI GCNGC 7 cut(s) 29, 32, 134, 712, 1074, 1209, 1257
HaeII RGCGCY 1 cut(s) 411
HaeIII GGCC 4 cut(s) 210, 492, 711, 1076
HapII CCGG 2 cut(s) 207, 402
HgaI GACGC 1 cut(s) 184
HhaI GCGC 6 cut(s) 46, 48, 170, 410, 442, 1218
Hin1I GRCGYC 2 cut(s) 23, 853
Hin1II CATG 5 cut(s) 541, 1067, 1115, 1216, 1281
Hin6I GCGC 6 cut(s) 44, 46, 168, 408, 440, 1216
HinP1I GCGC 6 cut(s) 44, 46, 168, 408, 440, 1216
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 4 cut(s) 461, 530, 784, 889
HpaII CCGG 2 cut(s) 207, 402
HphI GGTGA 3 cut(s) 16, 723, 1266
Hpy166II GTNNAC 4 cut(s) 465, 685, 860, 1117
Hpy188I TCNGA 7 cut(s) 78, 357, 613, 789, 875, 903, 1224
Hpy188III TCNNGA 2 cut(s) 328, 575
Hpy8I GTNNAC 4 cut(s) 465, 685, 860, 1117
Hpy99I CGWCG 3 cut(s) 28, 246, 858
HpyAV CCTTC 5 cut(s) 162, 235, 697, 978, 1229
HpyCH4III ACNGT 4 cut(s) 617, 771, 917, 1165
HpyCH4IV ACGT 4 cut(s) 23, 51, 244, 853
HpyCH4V TGCA 4 cut(s) 566, 849, 940, 1259
HpyF10VI GCNNNNNNNGC 7 cut(s) 207, 212, 221, 407, 946, 1073, 1224
HpyF3I CTNAG 2 cut(s) 129, 1223
HpySE526I ACGT 4 cut(s) 23, 51, 244, 853
Hsp92I GRCGYC 2 cut(s) 23, 853
Hsp92II CATG 5 cut(s) 541, 1067, 1115, 1216, 1281
HspAI GCGC 6 cut(s) 44, 46, 168, 408, 440, 1216
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 6 cut(s) 54, 78, 173, 580, 764, 1104
LmnI GCTCC 1 cut(s) 1008
Lsp1109I GCAGC 3 cut(s) 145, 1195, 1243
LweI GCATC 1 cut(s) 1054
MaeI CTAG 2 cut(s) 296, 672
MaeII ACGT 4 cut(s) 23, 51, 244, 853
MaeIII GTNAC 6 cut(s) 4, 245, 367, 498, 729, 1241
MalI GATC 6 cut(s) 56, 80, 175, 582, 766, 1106
MbiI CCGCTC 1 cut(s) 256
MboI GATC 6 cut(s) 54, 78, 173, 580, 764, 1104
MboII GAAGA 5 cut(s) 28, 31, 76, 147, 271
MfeI CAATTG 1 cut(s) 337
MflI RGATCY 2 cut(s) 173, 580
MluCI AATT 8 cut(s) 322, 331, 337, 376, 415, 652, 1130, 1156
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 1 cut(s) 335
MroNI GCCGGC 1 cut(s) 206
MseI TTAA 3 cut(s) 894, 992, 1265
MslI CAYNNNNRTG 3 cut(s) 515, 1068, 1276
MspA1I CMGCKG 2 cut(s) 66, 1256
MspI CCGG 2 cut(s) 207, 402
MspR9I CCNGG 2 cut(s) 444, 695
MunI CAATTG 1 cut(s) 337
Mva1269I GAATGC 1 cut(s) 403
MvaI CCWGG 2 cut(s) 444, 695
MvnI CGCG 4 cut(s) 46, 168, 170, 440
MwoI GCNNNNNNNGC 7 cut(s) 207, 212, 221, 407, 946, 1073, 1224
NaeI GCCGGC 1 cut(s) 208
NdeII GATC 6 cut(s) 54, 78, 173, 580, 764, 1104
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 5 cut(s) 541, 1067, 1115, 1216, 1281
NlaIV GGNNCC 3 cut(s) 211, 582, 1077
NmuCI GTSAC 5 cut(s) 4, 245, 367, 729, 1241
NspV TTCGAA 1 cut(s) 1135
PalAI GGCGCGCC 1 cut(s) 44
PauI GCGCGC 1 cut(s) 44
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 87
PctI GAATGC 1 cut(s) 403
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 3 cut(s) 530, 784, 889
PflFI GACNNNGTC 1 cut(s) 855
PfoI TCCNGGA 1 cut(s) 693
PkrI GCNGC 7 cut(s) 30, 33, 135, 713, 1075, 1210, 1258
PleI GAGTC 1 cut(s) 469
PmaCI CACGTG 1 cut(s) 52
PmlI CACGTG 1 cut(s) 52
PpsI GAGTC 1 cut(s) 469
Ppu21I YACGTR 1 cut(s) 52
Psp6I CCWGG 2 cut(s) 442, 693
PspCI CACGTG 1 cut(s) 52
PspGI CCWGG 2 cut(s) 442, 693
PspN4I GGNNCC 3 cut(s) 211, 582, 1077
PspPI GGNCC 4 cut(s) 60, 209, 709, 1075
PstNI CAGNNNCTG 1 cut(s) 946
PsuI RGATCY 2 cut(s) 173, 580
PsyI GACNNNGTC 1 cut(s) 855
PteI GCGCGC 1 cut(s) 44
PvuII CAGCTG 2 cut(s) 66, 1256
RsaI GTAC 2 cut(s) 116, 632
RsaNI GTAC 2 cut(s) 115, 631
RseI CAYNNNNRTG 3 cut(s) 515, 1068, 1276
SaqAI TTAA 3 cut(s) 894, 992, 1265
SatI GCNGC 7 cut(s) 29, 32, 134, 712, 1074, 1209, 1257
Sau3AI GATC 6 cut(s) 54, 78, 173, 580, 764, 1104
Sau96I GGNCC 4 cut(s) 60, 209, 709, 1075
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 444, 695
SfaNI GCATC 1 cut(s) 1054
SfuI TTCGAA 1 cut(s) 1135
SgsI GGCGCGCC 1 cut(s) 44
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 3 cut(s) 515, 1068, 1276
SmlI CTYRAG 2 cut(s) 575, 977
SmoI CTYRAG 2 cut(s) 575, 977
Sse9I AATT 8 cut(s) 322, 331, 337, 376, 415, 652, 1130, 1156
SsiI CCGC 9 cut(s) 29, 32, 213, 254, 438, 548, 712, 757, 1073
SspMI CTAG 2 cut(s) 296, 672
StyD4I CCNGG 2 cut(s) 442, 693
StyI CCWWGG 3 cut(s) 216, 671, 1200
TaaI ACNGT 4 cut(s) 617, 771, 917, 1165
TaiI ACGT 4 cut(s) 26, 54, 247, 856
TaqI TCGA 7 cut(s) 258, 282, 289, 422, 456, 533, 1135
TasI AATT 8 cut(s) 322, 331, 337, 376, 415, 652, 1130, 1156
TatI WGTACW 2 cut(s) 114, 630
TauI GCSGC 4 cut(s) 31, 34, 714, 1076
TfiI GAWTC 3 cut(s) 530, 784, 889
Tru1I TTAA 3 cut(s) 894, 992, 1265
Tru9I TTAA 3 cut(s) 894, 992, 1265
TscAI CASTG 2 cut(s) 776, 951
TseFI GTSAC 5 cut(s) 4, 245, 367, 729, 1241
TseI GCWGC 3 cut(s) 133, 1208, 1256
Tsp45I GTSAC 5 cut(s) 4, 245, 367, 729, 1241
TspDTI ATGAA 3 cut(s) 147, 634, 831
TspGWI ACGGA 3 cut(s) 79, 376, 386
TspRI CASTG 2 cut(s) 776, 951
Tth111I GACNNNGTC 1 cut(s) 855
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 6 cut(s) 322, 331, 376, 415, 1130, 1156
XcmI CCANNNNNNNNNTGG 1 cut(s) 750
XmaJI CCTAGG 1 cut(s) 671
XmiI GTMKAC 1 cut(s) 1116
XspI CTAG 2 cut(s) 296, 672
ZraI GACGTC 2 cut(s) 24, 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.