FvH4_6g42672

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002602
Physical Location & Seq
Forward (+)
33208683 .. 33211508
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42672.t2

Sequence Viewer

Length: 1134 bp
ATGACAAAGAGAAGGCCTTCATCACTGATGCGCCGACTTTCAAGGTTGAAGTCAAGACTCATCATCATCCTCTGTGTCTTGCTATGTGTACTGGCTTTCCTTAGATTAGTACTCCGTTCTGAGTCCAACCTCCAGTCCTCATTTCCCTCCACTCCAAGAAGGTCCCGTGGTTTTATTGATGCTCAATTTGTTGGCCCTCCAAAGGTTGCGTTTCTCTTCCTGGTTCGTCAATATCTCCCTCTTGATTTTCTCTGGGGCACCTTCTTCAAGAATGGTGATGCAGCAAAATTCTCCATTTACATTCATTCAGAGCCTGGTTTTGTGCTTGATGAAACCACCACAAGGTCTGCATTCTTCTTTGGTCGACAGTTAAACAACAGCATTCAGGTAGTATGGGGAGAATCAAGTATGATAGAAGCAGAGCAGTTGCTATTTGAAGAAGCTCTTTATGATCCAGCCAATCAAAGATTTGTCCTTCTCTCAGATAGCTGTGTACCTCTCCACAACTTTAGTTACATTTACAACTACATAATGTCTTCCCCCAATAGCTTCGTTGACAGCTTTATTGATGTTAAAGGACGTCGTTACGATCCAAAGATGTCACCTACTATACCTAAGGAGAGATGGCGAAAAGGATCCCAGTGGATAACATTGGTTAGAAAACATGCTGAAATTGTTGTGGATGATGATACTGTATTCCCACTGTTCAGGAAATTCTGCAAGCGCTGGCCACCTACACCCCTGAGTTTCAGAAGGCGACTACTTGCAATCAATTGGACTTCGAAGCTTCCGTTGTCTATATTTCAGAAGCATCGCAACTGTATCCCGGATGAACATTATGTGCAGACACTACTTGCAATAAGTGGACTTGACAACGAACTTGAACAGCGAGGAGTAACCTATACATTATGGAATCAGTCAACCATAAAAGGGAAAAAAAAATCTTGGCACCCCTTAACATTTGATTATGCAGATGCAGCTCCTCAAAAATTAAAAGAAATAAAGGAAATCAACCACGTCTACAATGAGTATACACAACACAATGAGTTCTGCCATACCAATTCAAAAGTCACTCCCTGTTTCTTGTTTGCGCGAAAGTTCACAGTTGGAGCTGCAATGCGCGTCTTGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

378

Amino Acids

44.03

Weight (kDa)

9.76

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 73 - 318 6.1e-60 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 583
AccB1I GGYRCC 2 cut(s) 257, 948
AccI GTMKAC 3 cut(s) 364, 1020, 1031
AccII CGCG 2 cut(s) 1093, 1122
AclWI GGATC 4 cut(s) 446, 584, 630, 643
AcoI YGGCCR 1 cut(s) 728
AcsI RAATTY 2 cut(s) 287, 713
AcyI GRCGYC 1 cut(s) 580
AfaI GTAC 3 cut(s) 90, 111, 495
AfeI AGCGCT 1 cut(s) 725
AfiI CCNNNNNNNGG 3 cut(s) 202, 342, 930
AgsI TTSAA 6 cut(s) 42, 49, 268, 437, 884, 1065
AjiI CACGTC 1 cut(s) 1018
AjnI CCWGG 2 cut(s) 219, 313
AluBI AGCT 7 cut(s) 443, 489, 549, 561, 787, 980, 1112
AluI AGCT 7 cut(s) 443, 489, 549, 561, 787, 980, 1112
AlwI GGATC 4 cut(s) 446, 584, 630, 643
AlwNI CAGNNNCTG 1 cut(s) 314
Aor51HI AGCGCT 1 cut(s) 725
AoxI GGCC 3 cut(s) 14, 193, 728
ApeKI GCWGC 3 cut(s) 281, 977, 1112
ApoI RAATTY 2 cut(s) 287, 713
Asp700I GAANNNNTTC 1 cut(s) 16
AspLEI GCGC 4 cut(s) 33, 726, 1093, 1122
AspS9I GGNCC 2 cut(s) 162, 194
AsuC2I CCSGG 1 cut(s) 827
AsuHPI GGTGA 2 cut(s) 287, 594
AsuII TTCGAA 1 cut(s) 782
AvaII GGWCC 1 cut(s) 162
AxyI CCTNAGG 1 cut(s) 615
BaeGI GKGCMC 1 cut(s) 260
BalI TGGCCA 1 cut(s) 730
BamHI GGATCC 1 cut(s) 635
BanI GGYRCC 2 cut(s) 257, 948
BbsI GAAGAC 1 cut(s) 528
BbvI GCAGC 3 cut(s) 293, 989, 1099
BccI CCATC 1 cut(s) 618
BciT130I CCWGG 2 cut(s) 221, 315
BciVI GTATCC 1 cut(s) 833
BcnI CCSGG 1 cut(s) 827
BfoI RGCGCY 1 cut(s) 727
BfuI GTATCC 1 cut(s) 833
BisI GCNGC 3 cut(s) 282, 978, 1113
BlsI GCNGC 3 cut(s) 283, 979, 1114
BmcAI AGTACT 1 cut(s) 111
Bme1390I CCNGG 3 cut(s) 221, 315, 827
Bme18I GGWCC 1 cut(s) 162
BmgBI CACGTC 1 cut(s) 1018
BmgT120I GGNCC 2 cut(s) 162, 194
BmiI GGNNCC 4 cut(s) 164, 259, 637, 950
BmrFI CCNGG 3 cut(s) 221, 315, 827
BmrI ACTGGG 1 cut(s) 634
BmsI GCATC 5 cut(s) 18, 169, 268, 820, 964
BmuI ACTGGG 1 cut(s) 634
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 116
Bpu14I TTCGAA 1 cut(s) 782
BpuMI CCSGG 1 cut(s) 827
BsaHI GRCGYC 1 cut(s) 580
BsaJI CCNNGG 1 cut(s) 166
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 342, 930
Bse1I ACTGG 3 cut(s) 96, 133, 640
Bse21I CCTNAGG 1 cut(s) 615
Bse3DI GCAATG 1 cut(s) 1122
BseBI CCWGG 2 cut(s) 221, 315
BseDI CCNNGG 1 cut(s) 166
BseGI GGATG 3 cut(s) 66, 688, 835
BseLI CCNNNNNNNGG 3 cut(s) 202, 342, 930
BseMI GCAATG 1 cut(s) 1122
BseMII CTCAG 3 cut(s) 111, 495, 734
BseNI ACTGG 3 cut(s) 96, 133, 640
BseRI GAGGAG 2 cut(s) 906, 972
BseSI GKGCMC 1 cut(s) 260
BseXI GCAGC 3 cut(s) 293, 989, 1099
BsgI GTGCAG 1 cut(s) 863
Bsh1236I CGCG 2 cut(s) 1093, 1122
BshFI GGCC 3 cut(s) 16, 195, 730
BshNI GGYRCC 2 cut(s) 257, 948
BsiSI CCGG 1 cut(s) 827
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 3 cut(s) 202, 342, 930
BsmFI GGGAC 1 cut(s) 148
BsmI GAATGC 2 cut(s) 350, 381
BsnI GGCC 3 cut(s) 16, 195, 730
Bsp119I TTCGAA 1 cut(s) 782
Bsp1286I GDGCHC 1 cut(s) 260
Bsp143I GATC 3 cut(s) 451, 589, 635
BspANI GGCC 3 cut(s) 16, 195, 730
BspCNI CTCAG 3 cut(s) 112, 494, 735
BspFNI CGCG 2 cut(s) 1093, 1122
BspLI GGNNCC 4 cut(s) 164, 259, 637, 950
BspPI GGATC 4 cut(s) 446, 584, 630, 643
BspT104I TTCGAA 1 cut(s) 782
BspT107I GGYRCC 2 cut(s) 257, 948
BsrDI GCAATG 1 cut(s) 1122
BsrI ACTGG 3 cut(s) 96, 133, 640
BssECI CCNNGG 1 cut(s) 166
BssMI GATC 3 cut(s) 451, 589, 635
BssNAI GTATAC 1 cut(s) 1032
BssNI GRCGYC 1 cut(s) 580
Bst1107I GTATAC 1 cut(s) 1032
Bst2UI CCWGG 2 cut(s) 221, 315
Bst4CI ACNGT 5 cut(s) 369, 694, 705, 821, 1105
Bst6I CTCTTC 1 cut(s) 221
BstACI GRCGYC 1 cut(s) 580
BstBI TTCGAA 1 cut(s) 782
BstC8I GCNNGC 2 cut(s) 722, 728
BstDEI CTNAG 5 cut(s) 101, 120, 481, 615, 743
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 3 cut(s) 66, 688, 835
BstFNI CGCG 2 cut(s) 1093, 1122
BstH2I RGCGCY 1 cut(s) 727
BstHHI GCGC 4 cut(s) 33, 726, 1093, 1122
BstKTI GATC 3 cut(s) 454, 592, 638
BstMBI GATC 3 cut(s) 451, 589, 635
BstMWI GCNNNNNNNGC 1 cut(s) 977
BstNI CCWGG 2 cut(s) 221, 315
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 3 cut(s) 219, 313, 825
BstSLI GKGCMC 1 cut(s) 260
BstUI CGCG 2 cut(s) 1093, 1122
BstV1I GCAGC 3 cut(s) 293, 989, 1099
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 1 cut(s) 635
BstYI RGATCY 1 cut(s) 635
BstZ17I GTATAC 1 cut(s) 1032
Bsu36I CCTNAGG 1 cut(s) 615
BsuI GTATCC 1 cut(s) 833
BsuRI GGCC 3 cut(s) 16, 195, 730
BtgI CCRYGG 1 cut(s) 166
BtgZI GCGATG 1 cut(s) 797
BtrI CACGTC 1 cut(s) 1018
BtsCI GGATG 3 cut(s) 66, 688, 835
BtsIMutI CAGTG 3 cut(s) 23, 647, 701
Cac8I GCNNGC 2 cut(s) 722, 728
CaiI CAGNNNCTG 1 cut(s) 314
CfoI GCGC 4 cut(s) 33, 726, 1093, 1122
Cfr13I GGNCC 2 cut(s) 162, 194
CseI GACGC 1 cut(s) 1111
Csp6I GTAC 3 cut(s) 89, 110, 494
CviAII CATG 1 cut(s) 665
CviQI GTAC 3 cut(s) 89, 110, 494
DdeI CTNAG 5 cut(s) 101, 120, 481, 615, 743
DpnI GATC 3 cut(s) 453, 591, 637
DpnII GATC 3 cut(s) 451, 589, 635
EaeI YGGCCR 1 cut(s) 728
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Eco147I AGGCCT 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 162
Eco47III AGCGCT 1 cut(s) 725
Eco81I CCTNAGG 1 cut(s) 615
EcoO109I RGGNCCY 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 219, 313
FaeI CATG 1 cut(s) 668
FalI AAGNNNNNCTT 2 cut(s) 429, 461
FaqI GGGAC 1 cut(s) 148
FatI CATG 1 cut(s) 664
FblI GTMKAC 3 cut(s) 364, 1020, 1031
Fnu4HI GCNGC 3 cut(s) 282, 978, 1113
FokI GGATG 3 cut(s) 53, 695, 842
Fsp4HI GCNGC 3 cut(s) 282, 978, 1113
GlaI GCGC 4 cut(s) 32, 725, 1092, 1121
GluI GCNGC 3 cut(s) 282, 978, 1113
GsuI CTGGAG 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 727
HaeIII GGCC 3 cut(s) 16, 195, 730
HapII CCGG 1 cut(s) 827
HgaI GACGC 1 cut(s) 1111
HhaI GCGC 4 cut(s) 33, 726, 1093, 1122
Hin1I GRCGYC 1 cut(s) 580
Hin1II CATG 1 cut(s) 668
Hin6I GCGC 4 cut(s) 31, 724, 1091, 1120
HinP1I GCGC 4 cut(s) 31, 724, 1091, 1120
HincII GTYRAC 3 cut(s) 365, 556, 921
HindII GTYRAC 3 cut(s) 365, 556, 921
HindIII AAGCTT 1 cut(s) 785
HinfI GANTC 4 cut(s) 57, 122, 401, 913
HpaII CCGG 1 cut(s) 827
HphI GGTGA 2 cut(s) 287, 594
Hpy166II GTNNAC 9 cut(s) 89, 365, 494, 556, 866, 921, 1021, 1032, 1101
Hpy188I TCNGA 5 cut(s) 121, 310, 484, 752, 807
Hpy188III TCNNGA 5 cut(s) 54, 242, 268, 709, 1126
Hpy8I GTNNAC 9 cut(s) 89, 365, 494, 556, 866, 921, 1021, 1032, 1101
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 6 cut(s) 6, 27, 153, 271, 485, 747
HpyCH4III ACNGT 5 cut(s) 369, 694, 705, 821, 1105
HpyCH4IV ACGT 2 cut(s) 580, 1017
HpyCH4V TGCA 9 cut(s) 281, 350, 720, 767, 844, 857, 971, 977, 1115
HpyF10VI GCNNNNNNNGC 1 cut(s) 977
HpyF3I CTNAG 5 cut(s) 101, 120, 481, 615, 743
HpySE526I ACGT 2 cut(s) 580, 1017
Hsp92I GRCGYC 1 cut(s) 580
Hsp92II CATG 1 cut(s) 668
HspAI GCGC 4 cut(s) 31, 724, 1091, 1120
Kzo9I GATC 3 cut(s) 451, 589, 635
LmnI GCTCC 2 cut(s) 985, 1109
Lsp1109I GCAGC 3 cut(s) 293, 989, 1099
LweI GCATC 5 cut(s) 18, 169, 268, 820, 964
MaeII ACGT 2 cut(s) 580, 1017
MaeIII GTNAC 5 cut(s) 512, 584, 600, 895, 1069
MalI GATC 3 cut(s) 453, 591, 637
MboI GATC 3 cut(s) 451, 589, 635
MboII GAAGA 5 cut(s) 208, 256, 346, 449, 528
MfeI CAATTG 1 cut(s) 772
MflI RGATCY 1 cut(s) 635
MhlI GDGCHC 1 cut(s) 260
MlsI TGGCCA 1 cut(s) 730
MluCI AATT 7 cut(s) 185, 287, 672, 713, 772, 989, 1060
MluNI TGGCCA 1 cut(s) 730
MlyI GAGTC 2 cut(s) 51, 131
MmeI TCCRAC 2 cut(s) 150, 1087
MnlI CCTC 9 cut(s) 80, 140, 148, 157, 207, 249, 507, 884, 993
Mox20I TGGCCA 1 cut(s) 730
MroXI GAANNNNTTC 1 cut(s) 16
MscI TGGCCA 1 cut(s) 730
MseI TTAA 4 cut(s) 371, 573, 956, 992
Msp20I TGGCCA 1 cut(s) 730
MspI CCGG 1 cut(s) 827
MspR9I CCNGG 3 cut(s) 221, 315, 827
MunI CAATTG 1 cut(s) 772
Mva1269I GAATGC 2 cut(s) 350, 381
MvaI CCWGG 2 cut(s) 221, 315
MvnI CGCG 2 cut(s) 1093, 1122
MwoI GCNNNNNNNGC 1 cut(s) 977
NciI CCSGG 1 cut(s) 827
NdeII GATC 3 cut(s) 451, 589, 635
NlaIII CATG 1 cut(s) 668
NlaIV GGNNCC 4 cut(s) 164, 259, 637, 950
NmuCI GTSAC 2 cut(s) 600, 1069
NspI RCATGY 1 cut(s) 668
NspV TTCGAA 1 cut(s) 782
PceI AGGCCT 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 788
PctI GAATGC 2 cut(s) 350, 381
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 2 cut(s) 401, 913
PfoI TCCNGGA 1 cut(s) 825
PkrI GCNGC 3 cut(s) 283, 979, 1114
PleI GAGTC 2 cut(s) 51, 130
PpsI GAGTC 2 cut(s) 51, 130
PpuMI RGGWCCY 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 219, 313
PspGI CCWGG 2 cut(s) 219, 313
PspN4I GGNNCC 4 cut(s) 164, 259, 637, 950
PspPI GGNCC 2 cut(s) 162, 194
PspPPI RGGWCCY 1 cut(s) 162
PstNI CAGNNNCTG 1 cut(s) 314
PsuI RGATCY 1 cut(s) 635
RsaI GTAC 3 cut(s) 90, 111, 495
RsaNI GTAC 3 cut(s) 89, 110, 494
SalI GTCGAC 1 cut(s) 363
SaqAI TTAA 4 cut(s) 371, 573, 956, 992
SatI GCNGC 3 cut(s) 282, 978, 1113
Sau3AI GATC 3 cut(s) 451, 589, 635
Sau96I GGNCC 2 cut(s) 162, 194
ScaI AGTACT 1 cut(s) 111
SchI GAGTC 2 cut(s) 51, 131
ScrFI CCNGG 3 cut(s) 221, 315, 827
SduI GDGCHC 1 cut(s) 260
SfaNI GCATC 5 cut(s) 18, 169, 268, 820, 964
SfuI TTCGAA 1 cut(s) 782
SinI GGWCC 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 1126
SmoI CTYRAG 1 cut(s) 1126
Sse9I AATT 7 cut(s) 185, 287, 672, 713, 772, 989, 1060
SseBI AGGCCT 1 cut(s) 16
StuI AGGCCT 1 cut(s) 16
StyD4I CCNGG 3 cut(s) 219, 313, 825
TaaI ACNGT 5 cut(s) 369, 694, 705, 821, 1105
TaiI ACGT 2 cut(s) 583, 1020
TaqI TCGA 2 cut(s) 364, 782
TasI AATT 7 cut(s) 185, 287, 672, 713, 772, 989, 1060
TatI WGTACW 2 cut(s) 88, 109
TfiI GAWTC 2 cut(s) 401, 913
Tru1I TTAA 4 cut(s) 371, 573, 956, 992
Tru9I TTAA 4 cut(s) 371, 573, 956, 992
TscAI CASTG 3 cut(s) 30, 647, 708
TseFI GTSAC 2 cut(s) 600, 1069
TseI GCWGC 3 cut(s) 281, 977, 1112
Tsp45I GTSAC 2 cut(s) 600, 1069
TspDTI ATGAA 4 cut(s) 9, 293, 345, 846
TspGWI ACGGA 2 cut(s) 104, 780
TspRI CASTG 3 cut(s) 30, 647, 708
VpaK11BI GGWCC 1 cut(s) 162
XapI RAATTY 2 cut(s) 287, 713
XceI RCATGY 1 cut(s) 668
XmiI GTMKAC 3 cut(s) 364, 1020, 1031
XmnI GAANNNNTTC 1 cut(s) 16
ZraI GACGTC 1 cut(s) 581
ZrmI AGTACT 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.