RLG00000021099

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002602
Physical Location & Seq
Forward (+)
71941543 .. 71943983
2441 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021099

Sequence Viewer

Length: 1182 bp
ATGACAAAGAGAAGGGCTTCATCACCGATGCGCCAACTTTCAAGGTTGAGGTCAAGACTCATCATCATCCTCTGTGTCTCGCTATGCGTACTGGCTTTACTTACAGTAGTATTCAATTCTGAGTCCAACCCTCAGTCCTCATTCCCCACCTCTCGAAGAAGGTCTCGTGCTTTTATTGATATTCAGTTTGTTGGCCCTCCAAAGGTTGCTTTTCTCTTCCTGGTTCGGCGGGATCTCCCTCTTGATTTTCTCTGGGGCACCTTCTTCAAGAATGGTGATGGAGCAAAATTCTCCATTTACGTTCATTCAGAGCCTGGTTTTGTGTTTGATGGATCAACCACAAGGTCTGCATTCTTCTTTGGTCGACAGTTAAATAACAGCATTCAGGTAGTATGGGGAGAATCAAGTATGATCGAAGCAGAGCGGTTACTATTTGAAGAAGCTCTTTATGATCCAGCCAATCAAAGATTTGTCCTTCTCTCAGATAGCTGTGTACCTCTCTACAACTTTAGTTACATTTACAACTATGTAATGTCTTCTCCAAAAAGCTTCGTTGACAGCTTTATTGATGTTAAAGGACATCGTTATGATCCAAAGATGTCACCTACTATACCTAATGAGAGATGGCGAAAAGGATCCCAGTGGATAACATTGGCTCGAAGACATGCTGAAATTGTTGTGGATGACGACACTGTTTTCCCCATCTTCAGGGAATTCTGCAAGCGATGGCCGCCTACACCCCTGAGTTTTAGAAGGCGACTACTTGCAATCAATTGGACTTTCAAGCTTCCATTGTTTACATTTCAGAGGCATCGCAATTGTATCCCCGATGAACATTATGTGCAGACACTACTTGCAATAAGTGGACTTGACAACGAACTTGAACGAAGAGCAGTGACCTATACATCATGGAATCAGTCAACCCTAGAAGGGAAAAGAAGATCTTGGCACCCTTTAACATTTGATTATGCAGATGCAGCTCCTCAAAAACTGAGACACATAAAGGAAATCAACCACGTCTACAATGAGTATGAACAACACAATGAGTTCTGCCATACCAATTCCAAAGTCACTCCTTGTTTCTTGTTTGCACGAAAGTTCACTCTTGGAGCTGCAATGCGCGTCTTGAGAGAAGGTTTGGTTGGTTCTTATGATGTGACAGCAATACTACGAGATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

394

Amino Acids

45.86

Weight (kDa)

9.56

Isoelectric Point (pI)

61.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 69 - 334 1.7e-66 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 257, 948
AccBSI CCGCTC 1 cut(s) 424
AccI GTMKAC 2 cut(s) 364, 1020
AccII CGCG 1 cut(s) 1122
AciI CCGC 3 cut(s) 229, 424, 731
AclWI GGATC 6 cut(s) 240, 340, 446, 584, 630, 643
AcoI YGGCCR 1 cut(s) 728
AcsI RAATTY 2 cut(s) 287, 713
AcuI CTGAAG 1 cut(s) 691
AfaI GTAC 2 cut(s) 90, 495
AfiI CCNNNNNNNGG 3 cut(s) 202, 708, 930
AgsI TTSAA 6 cut(s) 42, 115, 268, 437, 784, 884
AjiI CACGTC 1 cut(s) 1018
AjnI CCWGG 2 cut(s) 219, 313
AjuI GAANNNNNNNTTGG 2 cut(s) 1125, 1157
AluBI AGCT 7 cut(s) 443, 489, 549, 561, 787, 980, 1112
AluI AGCT 7 cut(s) 443, 489, 549, 561, 787, 980, 1112
Alw26I GTCTC 3 cut(s) 82, 168, 988
AlwI GGATC 6 cut(s) 240, 340, 446, 584, 630, 643
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 2 cut(s) 193, 728
ApeKI GCWGC 2 cut(s) 977, 1112
ApoI RAATTY 2 cut(s) 287, 713
Asp700I GAANNNNTTC 1 cut(s) 16
AspLEI GCGC 2 cut(s) 33, 1122
AspS9I GGNCC 1 cut(s) 194
AsuHPI GGTGA 3 cut(s) 15, 287, 594
BaeGI GKGCMC 1 cut(s) 260
BamHI GGATCC 1 cut(s) 635
BanI GGYRCC 2 cut(s) 257, 948
BauI CACGAG 1 cut(s) 165
BbsI GAAGAC 2 cut(s) 528, 667
BbvI GCAGC 2 cut(s) 989, 1099
BccI CCATC 5 cut(s) 272, 323, 618, 710, 720
BciT130I CCWGG 2 cut(s) 221, 315
BciVI GTATCC 1 cut(s) 833
BcoDI GTCTC 3 cut(s) 82, 168, 988
BfaI CTAG 2 cut(s) 926, 1180
BfuI GTATCC 1 cut(s) 833
BglII AGATCT 1 cut(s) 941
BisI GCNGC 3 cut(s) 731, 978, 1113
BlsI GCNGC 3 cut(s) 732, 979, 1114
Bme1390I CCNGG 2 cut(s) 221, 315
BmgBI CACGTC 1 cut(s) 1018
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 3 cut(s) 259, 637, 950
BmrFI CCNGG 2 cut(s) 221, 315
BmrI ACTGGG 1 cut(s) 634
BmsI GCATC 3 cut(s) 18, 820, 964
BmuI ACTGGG 1 cut(s) 634
BpiI GAAGAC 2 cut(s) 528, 667
BpuEI CTTGAG 1 cut(s) 1147
BsaI GGTCTC 1 cut(s) 168
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 708, 930
Bse1I ACTGG 2 cut(s) 96, 640
Bse3DI GCAATG 1 cut(s) 1122
BseBI CCWGG 2 cut(s) 221, 315
BseGI GGATG 2 cut(s) 66, 688
BseLI CCNNNNNNNGG 3 cut(s) 202, 708, 930
BseMI GCAATG 1 cut(s) 1122
BseMII CTCAG 5 cut(s) 111, 146, 495, 734, 983
BseNI ACTGG 2 cut(s) 96, 640
BseRI GAGGAG 1 cut(s) 972
BseSI GKGCMC 1 cut(s) 260
BseXI GCAGC 2 cut(s) 989, 1099
BsgI GTGCAG 1 cut(s) 863
Bsh1236I CGCG 1 cut(s) 1122
BshFI GGCC 2 cut(s) 195, 730
BshNI GGYRCC 2 cut(s) 257, 948
BslI CCNNNNNNNGG 3 cut(s) 202, 708, 930
BsmAI GTCTC 3 cut(s) 82, 168, 988
BsmI GAATGC 2 cut(s) 350, 381
BsnI GGCC 2 cut(s) 195, 730
Bso31I GGTCTC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 260
Bsp143I GATC 7 cut(s) 232, 332, 411, 451, 589, 635, 941
BspACI CCGC 3 cut(s) 229, 424, 731
BspANI GGCC 2 cut(s) 195, 730
BspCNI CTCAG 5 cut(s) 112, 145, 494, 735, 984
BspFNI CGCG 1 cut(s) 1122
BspLI GGNNCC 3 cut(s) 259, 637, 950
BspPI GGATC 6 cut(s) 240, 340, 446, 584, 630, 643
BspQI GCTCTTC 1 cut(s) 883
BspT107I GGYRCC 2 cut(s) 257, 948
BspTNI GGTCTC 1 cut(s) 168
BsrBI CCGCTC 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 1122
BsrI ACTGG 2 cut(s) 96, 640
BssMI GATC 7 cut(s) 232, 332, 411, 451, 589, 635, 941
BssSI CACGAG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 221, 315
Bst4CI ACNGT 3 cut(s) 106, 369, 694
Bst6I CTCTTC 2 cut(s) 221, 883
BstC8I GCNNGC 1 cut(s) 722
BstDEI CTNAG 5 cut(s) 120, 132, 481, 743, 992
BstF5I GGATG 2 cut(s) 66, 688
BstFNI CGCG 1 cut(s) 1122
BstHHI GCGC 2 cut(s) 33, 1122
BstKTI GATC 7 cut(s) 235, 335, 414, 454, 592, 638, 944
BstMAI GTCTC 3 cut(s) 82, 168, 988
BstMBI GATC 7 cut(s) 232, 332, 411, 451, 589, 635, 941
BstMWI GCNNNNNNNGC 2 cut(s) 730, 977
BstNI CCWGG 2 cut(s) 221, 315
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 2 cut(s) 219, 313
BstSLI GKGCMC 1 cut(s) 260
BstUI CGCG 1 cut(s) 1122
BstV1I GCAGC 2 cut(s) 989, 1099
BstV2I GAAGAC 2 cut(s) 528, 667
BstX2I RGATCY 3 cut(s) 232, 635, 941
BstYI RGATCY 3 cut(s) 232, 635, 941
BsuI GTATCC 1 cut(s) 833
BsuRI GGCC 2 cut(s) 195, 730
BtgZI GCGATG 2 cut(s) 739, 797
BtrI CACGTC 1 cut(s) 1018
BtsCI GGATG 2 cut(s) 66, 688
BtsI GCAGTG 1 cut(s) 900
BtsIMutI CAGTG 3 cut(s) 647, 690, 900
Cac8I GCNNGC 1 cut(s) 722
CaiI CAGNNNCTG 1 cut(s) 314
CfoI GCGC 2 cut(s) 33, 1122
Cfr13I GGNCC 1 cut(s) 194
CseI GACGC 1 cut(s) 1111
Csp6I GTAC 2 cut(s) 89, 494
CviAII CATG 2 cut(s) 665, 909
CviQI GTAC 2 cut(s) 89, 494
DdeI CTNAG 5 cut(s) 120, 132, 481, 743, 992
DpnI GATC 7 cut(s) 234, 334, 413, 453, 591, 637, 943
DpnII GATC 7 cut(s) 232, 332, 411, 451, 589, 635, 941
EaeI YGGCCR 1 cut(s) 728
Eam1104I CTCTTC 2 cut(s) 221, 883
EarI CTCTTC 2 cut(s) 221, 883
Eco31I GGTCTC 1 cut(s) 168
Eco32I GATATC 1 cut(s) 1177
Eco57I CTGAAG 1 cut(s) 691
EcoRI GAATTC 1 cut(s) 713
EcoRII CCWGG 2 cut(s) 219, 313
EcoRV GATATC 1 cut(s) 1177
FaeI CATG 2 cut(s) 668, 912
FalI AAGNNNNNCTT 4 cut(s) 429, 461, 928, 960
FatI CATG 2 cut(s) 664, 908
FauI CCCGC 1 cut(s) 222
FblI GTMKAC 2 cut(s) 364, 1020
Fnu4HI GCNGC 3 cut(s) 731, 978, 1113
FokI GGATG 2 cut(s) 53, 695
Fsp4HI GCNGC 3 cut(s) 731, 978, 1113
FspBI CTAG 2 cut(s) 926, 1180
GlaI GCGC 2 cut(s) 32, 1121
GluI GCNGC 3 cut(s) 731, 978, 1113
HaeIII GGCC 2 cut(s) 195, 730
HgaI GACGC 1 cut(s) 1111
HhaI GCGC 2 cut(s) 33, 1122
Hin1II CATG 2 cut(s) 668, 912
Hin6I GCGC 2 cut(s) 31, 1120
HinP1I GCGC 2 cut(s) 31, 1120
HincII GTYRAC 3 cut(s) 365, 556, 921
HindII GTYRAC 3 cut(s) 365, 556, 921
HindIII AAGCTT 2 cut(s) 547, 785
HinfI GANTC 4 cut(s) 57, 122, 401, 913
HphI GGTGA 3 cut(s) 15, 287, 594
Hpy166II GTNNAC 8 cut(s) 365, 494, 556, 798, 866, 921, 1021, 1101
Hpy188I TCNGA 4 cut(s) 121, 310, 484, 807
Hpy188III TCNNGA 5 cut(s) 54, 153, 242, 268, 1126
Hpy8I GTNNAC 8 cut(s) 365, 494, 556, 798, 866, 921, 1021, 1101
HpyAV CCTTC 7 cut(s) 6, 153, 271, 485, 747, 923, 1127
HpyCH4III ACNGT 3 cut(s) 106, 369, 694
HpyCH4IV ACGT 2 cut(s) 300, 1017
HpyCH4V TGCA 9 cut(s) 350, 720, 767, 844, 857, 971, 977, 1091, 1115
HpyF10VI GCNNNNNNNGC 2 cut(s) 730, 977
HpyF3I CTNAG 5 cut(s) 120, 132, 481, 743, 992
HpySE526I ACGT 2 cut(s) 300, 1017
Hsp92II CATG 2 cut(s) 668, 912
HspAI GCGC 2 cut(s) 31, 1120
Kzo9I GATC 7 cut(s) 232, 332, 411, 451, 589, 635, 941
LguI GCTCTTC 1 cut(s) 883
LmnI GCTCC 3 cut(s) 281, 985, 1109
Lsp1109I GCAGC 2 cut(s) 989, 1099
LweI GCATC 3 cut(s) 18, 820, 964
MaeI CTAG 2 cut(s) 926, 1180
MaeII ACGT 2 cut(s) 300, 1017
MaeIII GTNAC 6 cut(s) 426, 512, 600, 895, 1069, 1156
MalI GATC 7 cut(s) 234, 334, 413, 453, 591, 637, 943
MbiI CCGCTC 1 cut(s) 424
MboI GATC 7 cut(s) 232, 332, 411, 451, 589, 635, 941
MfeI CAATTG 2 cut(s) 772, 817
MflI RGATCY 3 cut(s) 232, 635, 941
MhlI GDGCHC 1 cut(s) 260
MluCI AATT 7 cut(s) 115, 287, 672, 713, 772, 817, 1060
MlyI GAGTC 2 cut(s) 51, 131
MmeI TCCRAC 1 cut(s) 150
MroXI GAANNNNTTC 1 cut(s) 16
MseI TTAA 3 cut(s) 371, 573, 956
MslI CAYNNNNRTG 1 cut(s) 585
MspR9I CCNGG 2 cut(s) 221, 315
MunI CAATTG 2 cut(s) 772, 817
Mva1269I GAATGC 2 cut(s) 350, 381
MvaI CCWGG 2 cut(s) 221, 315
MvnI CGCG 1 cut(s) 1122
MwoI GCNNNNNNNGC 2 cut(s) 730, 977
NdeII GATC 7 cut(s) 232, 332, 411, 451, 589, 635, 941
NlaIII CATG 2 cut(s) 668, 912
NlaIV GGNNCC 3 cut(s) 259, 637, 950
NmuCI GTSAC 4 cut(s) 600, 895, 1069, 1156
NspI RCATGY 1 cut(s) 668
PciSI GCTCTTC 1 cut(s) 883
PctI GAATGC 2 cut(s) 350, 381
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 2 cut(s) 401, 913
PkrI GCNGC 3 cut(s) 732, 979, 1114
PleI GAGTC 2 cut(s) 51, 130
PpsI GAGTC 2 cut(s) 51, 130
Psp6I CCWGG 2 cut(s) 219, 313
PspGI CCWGG 2 cut(s) 219, 313
PspN4I GGNNCC 3 cut(s) 259, 637, 950
PspPI GGNCC 1 cut(s) 194
PstNI CAGNNNCTG 1 cut(s) 314
PsuI RGATCY 3 cut(s) 232, 635, 941
RsaI GTAC 2 cut(s) 90, 495
RsaNI GTAC 2 cut(s) 89, 494
RseI CAYNNNNRTG 1 cut(s) 585
SalI GTCGAC 1 cut(s) 363
SapI GCTCTTC 1 cut(s) 883
SaqAI TTAA 3 cut(s) 371, 573, 956
SatI GCNGC 3 cut(s) 731, 978, 1113
Sau3AI GATC 7 cut(s) 232, 332, 411, 451, 589, 635, 941
Sau96I GGNCC 1 cut(s) 194
SchI GAGTC 2 cut(s) 51, 131
ScrFI CCNGG 2 cut(s) 221, 315
SduI GDGCHC 1 cut(s) 260
SfaNI GCATC 3 cut(s) 18, 820, 964
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 1 cut(s) 1126
SmoI CTYRAG 1 cut(s) 1126
Sse9I AATT 7 cut(s) 115, 287, 672, 713, 772, 817, 1060
SsiI CCGC 3 cut(s) 229, 424, 731
SspMI CTAG 2 cut(s) 926, 1180
StyD4I CCNGG 2 cut(s) 219, 313
TaaI ACNGT 3 cut(s) 106, 369, 694
TaiI ACGT 2 cut(s) 303, 1020
TaqI TCGA 4 cut(s) 154, 364, 414, 658
TasI AATT 7 cut(s) 115, 287, 672, 713, 772, 817, 1060
TauI GCSGC 1 cut(s) 733
TfiI GAWTC 2 cut(s) 401, 913
Tru1I TTAA 3 cut(s) 371, 573, 956
Tru9I TTAA 3 cut(s) 371, 573, 956
TscAI CASTG 3 cut(s) 647, 697, 900
TseFI GTSAC 4 cut(s) 600, 895, 1069, 1156
TseI GCWGC 2 cut(s) 977, 1112
Tsp45I GTSAC 4 cut(s) 600, 895, 1069, 1156
TspDTI ATGAA 4 cut(s) 9, 293, 846, 1047
TspRI CASTG 3 cut(s) 647, 697, 900
XapI RAATTY 2 cut(s) 287, 713
XceI RCATGY 1 cut(s) 668
XmiI GTMKAC 2 cut(s) 364, 1020
XmnI GAANNNNTTC 1 cut(s) 16
XspI CTAG 2 cut(s) 926, 1180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.