MD09G1071200.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002602
Physical Location & Seq
Forward (+)
4918665 .. 4921375
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1071200.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGGCCAAGAAGAGAGCTTCTTCAGCATCCATGAGCCGGCTTTTGAGCTTCGTTTCAAGGCTATTCATCGTTCTCAGCATCTTGCTATGTCTATTGGCTTTCCTTAGACTAATCCATTCTCAGTCCAAGGACCAACCACCAGTCTTTGCATCCCCAACAAGGTTTCCCGCTGTTATTCATGATAATCAGTTTGAAGGCCCTCCAAAAGTTGCTTTCCTCTTCCTTGCCCGCCGGGATCTTCCTCTTGATTTTCTTTGGAACACCTTCTTCAAGAATGGTGATGCGGCAAATTTCTCGATTTATATCCATTCGGAGCCTGGTTTTGTGTTTGATGAACCAACCACAAGGTCTGCTTTCTTTCATGATCGACAGTTGAACAACAGCATTCAGGTAGGGTGGGGGGAATCGAGCATGATAGAAGCAGAGCGCATACTACTCCAAGAAGCTCTGCGCGATCCAGCCAATCAAAGATTTGTCCTCCTTTCAGACAGCTGTGTACCTCTATACAACTTCAGTTACATTTACAACTACACAATCTCTTCTCCGAAAAGCTTTGTAGACAGCTTTGTCGATGGTCATGCATTTCGTTATGATCCGAAGATGGCACCTACCATACCTAAGGAAAAATGGCGTAAAGGATCCCAGTGGATAGCTTTGGTGAGAAGACATGCAGAAATCATCGTCAATGACAAGACCGTCTTTCCTGTCTTCAGGAAACTCTGTAAGCGATGGCCGCCTGGAGATTTAAAATGGAGGAGGAAACTTCTTGCATTCAATTTCGATATCAAGTATATATTGCAGACATTTGAGCAAAAACGCAATTGTATCCCCGATGAACATTATGTCCAGACACTACTTGCAATAAGTGGACTCGAGGACGAACTTGAACGAAGAGCACTGACCTACACATCATGGAATCAGTCAGCGGTAGGAAGTAAAAAGAGATCTTGGCACCCTATAAAGTTTGATTTTGCAGATGCAGGGCCTCAAAAGATCAGGGAAATTAAGGATATCGATCATGTATACGACGAGTCCGAGGGCCAAACAGAGTTCTGCCGCGCAAATTCCAAAGTCGCTCCTTGCTACTTGTTTGCAAGAAAATTCACTCTTGGAGCTGCTGTGCGGATTATGAGAGAAGGTGTGGTTGGTCCTTATGATGTTCCTTCAAAACCAAATATATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

395

Amino Acids

45.55

Weight (kDa)

9.34

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 70 - 335 3.7e-68 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 566, 695
AccB1I GGYRCC 2 cut(s) 604, 951
AccI GTMKAC 2 cut(s) 558, 1023
AccII CGCG 2 cut(s) 453, 1059
AciI CCGC 7 cut(s) 168, 229, 284, 734, 926, 1057, 1123
AclWI GGATC 5 cut(s) 243, 449, 587, 633, 646
AcoI YGGCCR 2 cut(s) 3, 731
AcsI RAATTY 3 cut(s) 289, 1063, 1100
AcuI CTGAAG 3 cut(s) 6, 496, 694
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 2 cut(s) 36, 159
AgsI TTSAA 7 cut(s) 57, 194, 271, 376, 775, 887, 1167
AjnI CCWGG 2 cut(s) 316, 736
AjuI GAANNNNNNNTTGG 2 cut(s) 1128, 1160
AloI GAACNNNNNNTCC 2 cut(s) 828, 860
AluBI AGCT 8 cut(s) 17, 48, 446, 492, 552, 564, 653, 1115
AluI AGCT 8 cut(s) 17, 48, 446, 492, 552, 564, 653, 1115
Alw21I GWGCWC 1 cut(s) 898
AlwI GGATC 5 cut(s) 243, 449, 587, 633, 646
Ama87I CYCGRG 1 cut(s) 872
AoxI GGCC 5 cut(s) 3, 196, 731, 983, 1039
ApeKI GCWGC 1 cut(s) 1115
ApoI RAATTY 3 cut(s) 289, 1063, 1100
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 3 cut(s) 429, 453, 1061
AspS9I GGNCC 5 cut(s) 130, 197, 983, 1039, 1148
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 2 cut(s) 290, 670
AvaI CYCGRG 1 cut(s) 872
AvaII GGWCC 2 cut(s) 130, 1148
AxyI CCTNAGG 1 cut(s) 618
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 638
BanI GGYRCC 2 cut(s) 604, 951
BbsI GAAGAC 2 cut(s) 670, 700
Bbv12I GWGCWC 1 cut(s) 898
BbvI GCAGC 1 cut(s) 1102
BccI CCATC 3 cut(s) 566, 595, 723
BcgI CGANNNNNNTGC 4 cut(s) 276, 310, 560, 594
BciT130I CCWGG 2 cut(s) 318, 738
BciVI GTATCC 1 cut(s) 836
BcnI CCSGG 1 cut(s) 233
BfuI GTATCC 1 cut(s) 836
BglII AGATCT 1 cut(s) 944
BisI GCNGC 4 cut(s) 285, 734, 1057, 1116
BlsI GCNGC 4 cut(s) 286, 735, 1058, 1117
Bme1390I CCNGG 3 cut(s) 233, 318, 738
Bme18I GGWCC 2 cut(s) 130, 1148
BmeT110I CYCGRG 1 cut(s) 872
BmgT120I GGNCC 5 cut(s) 130, 197, 983, 1039, 1148
BmiI GGNNCC 4 cut(s) 315, 606, 640, 953
BmrFI CCNGG 3 cut(s) 233, 318, 738
BmrI ACTGGG 1 cut(s) 637
BmsI GCATC 5 cut(s) 35, 87, 158, 271, 967
BmuI ACTGGG 1 cut(s) 637
BpiI GAAGAC 2 cut(s) 670, 700
BpmI CTGGAG 1 cut(s) 759
BpuMI CCSGG 1 cut(s) 233
Bsa29I ATCGAT 1 cut(s) 1014
BsaBI GATNNNNATC 2 cut(s) 302, 1014
BsaJI CCNNGG 2 cut(s) 126, 1035
BsaXI ACNNNNNCTCC 2 cut(s) 1104, 1134
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 159
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 2 cut(s) 140, 643
Bse21I CCTNAGG 1 cut(s) 618
Bse8I GATNNNNATC 2 cut(s) 302, 1014
BseBI CCWGG 2 cut(s) 318, 738
BseCI ATCGAT 1 cut(s) 1014
BseDI CCNNGG 2 cut(s) 126, 1035
BseGI GGATG 2 cut(s) 26, 149
BseJI GATNNNNATC 2 cut(s) 302, 1014
BseLI CCNNNNNNNGG 2 cut(s) 36, 159
BseMII CTCAG 2 cut(s) 88, 134
BseNI ACTGG 2 cut(s) 140, 643
BseRI GAGGAG 1 cut(s) 769
BseXI GCAGC 1 cut(s) 1102
Bsh1236I CGCG 2 cut(s) 453, 1059
BshFI GGCC 5 cut(s) 5, 198, 733, 985, 1041
BshNI GGYRCC 2 cut(s) 604, 951
BshVI ATCGAT 1 cut(s) 1014
BsiHKAI GWGCWC 1 cut(s) 898
BsiHKCI CYCGRG 1 cut(s) 872
BsiSI CCGG 2 cut(s) 37, 232
BslI CCNNNNNNNGG 2 cut(s) 36, 159
BsmI GAATGC 2 cut(s) 384, 770
BsnI GGCC 5 cut(s) 5, 198, 733, 985, 1041
BsoBI CYCGRG 1 cut(s) 872
Bsp1286I GDGCHC 1 cut(s) 898
Bsp143I GATC 8 cut(s) 235, 364, 454, 592, 638, 944, 993, 1015
BspACI CCGC 7 cut(s) 168, 229, 284, 734, 926, 1057, 1123
BspANI GGCC 5 cut(s) 5, 198, 733, 985, 1041
BspCNI CTCAG 2 cut(s) 87, 133
BspDI ATCGAT 1 cut(s) 1014
BspFNI CGCG 2 cut(s) 453, 1059
BspHI TCATGA 2 cut(s) 178, 361
BspLI GGNNCC 4 cut(s) 315, 606, 640, 953
BspPI GGATC 5 cut(s) 243, 449, 587, 633, 646
BspQI GCTCTTC 1 cut(s) 886
BspT107I GGYRCC 2 cut(s) 604, 951
BsrFI RCCGGY 1 cut(s) 36
BsrI ACTGG 2 cut(s) 140, 643
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 2 cut(s) 126, 1035
BssMI GATC 8 cut(s) 235, 364, 454, 592, 638, 944, 993, 1015
BssNAI GTATAC 1 cut(s) 1024
BssT1I CCWWGG 1 cut(s) 126
Bst1107I GTATAC 1 cut(s) 1024
Bst2UI CCWGG 2 cut(s) 318, 738
Bst4CI ACNGT 2 cut(s) 372, 697
Bst6I CTCTTC 4 cut(s) 5, 224, 544, 886
BstC8I GCNNGC 2 cut(s) 38, 229
BstDEI CTNAG 4 cut(s) 74, 104, 120, 618
BstF5I GGATG 2 cut(s) 26, 149
BstFNI CGCG 2 cut(s) 453, 1059
BstHHI GCGC 3 cut(s) 429, 453, 1061
BstKTI GATC 8 cut(s) 238, 367, 457, 595, 641, 947, 996, 1018
BstMBI GATC 8 cut(s) 235, 364, 454, 592, 638, 944, 993, 1015
BstMWI GCNNNNNNNGC 2 cut(s) 23, 733
BstNI CCWGG 2 cut(s) 318, 738
BstNSI RCATGY 1 cut(s) 671
BstSCI CCNGG 3 cut(s) 231, 316, 736
BstUI CGCG 2 cut(s) 453, 1059
BstV1I GCAGC 1 cut(s) 1102
BstV2I GAAGAC 2 cut(s) 670, 700
BstX2I RGATCY 3 cut(s) 235, 638, 944
BstYI RGATCY 3 cut(s) 235, 638, 944
BstZ17I GTATAC 1 cut(s) 1024
Bsu15I ATCGAT 1 cut(s) 1014
Bsu36I CCTNAGG 1 cut(s) 618
BsuI GTATCC 1 cut(s) 836
BsuRI GGCC 5 cut(s) 5, 198, 733, 985, 1041
BsuTUI ATCGAT 1 cut(s) 1014
BtgZI GCGATG 1 cut(s) 742
BtsCI GGATG 2 cut(s) 26, 149
BtsIMutI CAGTG 2 cut(s) 650, 896
Cac8I GCNNGC 2 cut(s) 38, 229
CciI TCATGA 2 cut(s) 178, 361
CfoI GCGC 3 cut(s) 429, 453, 1061
Cfr10I RCCGGY 1 cut(s) 36
Cfr13I GGNCC 5 cut(s) 130, 197, 983, 1039, 1148
ClaI ATCGAT 1 cut(s) 1014
Csp6I GTAC 1 cut(s) 497
CviAII CATG 8 cut(s) 31, 179, 362, 412, 578, 668, 912, 1019
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 4 cut(s) 74, 104, 120, 618
DpnI GATC 8 cut(s) 237, 366, 456, 594, 640, 946, 995, 1017
DpnII GATC 8 cut(s) 235, 364, 454, 592, 638, 944, 993, 1015
DraI TTTAAA 1 cut(s) 747
DrdI GACNNNNNNGTC 2 cut(s) 566, 695
DseDI GACNNNNNNGTC 2 cut(s) 566, 695
EaeI YGGCCR 2 cut(s) 3, 731
Eam1104I CTCTTC 4 cut(s) 5, 224, 544, 886
EarI CTCTTC 4 cut(s) 5, 224, 544, 886
Eco130I CCWWGG 1 cut(s) 126
Eco32I GATATC 2 cut(s) 784, 1012
Eco47I GGWCC 2 cut(s) 130, 1148
Eco57I CTGAAG 3 cut(s) 6, 496, 694
Eco81I CCTNAGG 1 cut(s) 618
Eco88I CYCGRG 1 cut(s) 872
EcoO109I RGGNCCY 2 cut(s) 197, 983
EcoRII CCWGG 2 cut(s) 316, 736
EcoRV GATATC 2 cut(s) 784, 1012
EcoT14I CCWWGG 1 cut(s) 126
EcoT22I ATGCAT 1 cut(s) 583
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 8 cut(s) 34, 182, 365, 415, 581, 671, 915, 1022
FalI AAGNNNNNCTT 4 cut(s) 337, 369, 683, 715
FatI CATG 8 cut(s) 30, 178, 361, 411, 577, 667, 911, 1018
FauI CCCGC 2 cut(s) 175, 236
FblI GTMKAC 2 cut(s) 558, 1023
Fnu4HI GCNGC 4 cut(s) 285, 734, 1057, 1116
FokI GGATG 2 cut(s) 13, 136
Fsp4HI GCNGC 4 cut(s) 285, 734, 1057, 1116
GlaI GCGC 3 cut(s) 428, 452, 1060
GluI GCNGC 4 cut(s) 285, 734, 1057, 1116
GsuI CTGGAG 1 cut(s) 759
HaeIII GGCC 5 cut(s) 5, 198, 733, 985, 1041
HapII CCGG 2 cut(s) 37, 232
HhaI GCGC 3 cut(s) 429, 453, 1061
Hin1II CATG 8 cut(s) 34, 182, 365, 415, 581, 671, 915, 1022
Hin6I GCGC 3 cut(s) 427, 451, 1059
HinP1I GCGC 3 cut(s) 427, 451, 1059
HindIII AAGCTT 1 cut(s) 550
HinfI GANTC 4 cut(s) 404, 870, 916, 1031
HpaII CCGG 2 cut(s) 37, 232
HphI GGTGA 2 cut(s) 290, 670
Hpy166II GTNNAC 4 cut(s) 497, 559, 869, 1024
Hpy188I TCNGA 5 cut(s) 313, 487, 546, 597, 1036
Hpy188III TCNNGA 7 cut(s) 179, 245, 271, 295, 362, 712, 847
Hpy8I GTNNAC 4 cut(s) 497, 559, 869, 1024
Hpy99I CGWCG 1 cut(s) 1031
HpyAV CCTTC 4 cut(s) 188, 274, 1130, 1173
HpyCH4III ACNGT 2 cut(s) 372, 697
HpyCH4V TGCA 9 cut(s) 149, 581, 671, 770, 799, 860, 974, 980, 1094
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 733
HpyF3I CTNAG 4 cut(s) 74, 104, 120, 618
Hsp92II CATG 8 cut(s) 34, 182, 365, 415, 581, 671, 915, 1022
HspAI GCGC 3 cut(s) 427, 451, 1059
KroI GCCGGC 1 cut(s) 36
KroNI GCCGGC 1 cut(s) 38
Kzo9I GATC 8 cut(s) 235, 364, 454, 592, 638, 944, 993, 1015
LguI GCTCTTC 1 cut(s) 886
LmnI GCTCC 3 cut(s) 313, 1081, 1112
Lsp1109I GCAGC 1 cut(s) 1102
LweI GCATC 5 cut(s) 35, 87, 158, 271, 967
MaeIII GTNAC 1 cut(s) 515
MalI GATC 8 cut(s) 237, 366, 456, 594, 640, 946, 995, 1017
MboI GATC 8 cut(s) 235, 364, 454, 592, 638, 944, 993, 1015
MfeI CAATTG 1 cut(s) 820
MflI RGATCY 3 cut(s) 235, 638, 944
MhlI GDGCHC 1 cut(s) 898
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 289, 775, 820, 1002, 1063, 1100
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 864, 1040
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 583
MroNI GCCGGC 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 263
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 746, 1005
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 170, 492, 926
MspI CCGG 2 cut(s) 37, 232
MspR9I CCNGG 3 cut(s) 233, 318, 738
MunI CAATTG 1 cut(s) 820
Mva1269I GAATGC 2 cut(s) 384, 770
MvaI CCWGG 2 cut(s) 318, 738
MvnI CGCG 2 cut(s) 453, 1059
MwoI GCNNNNNNNGC 2 cut(s) 23, 733
NaeI GCCGGC 1 cut(s) 38
NciI CCSGG 1 cut(s) 233
NdeII GATC 8 cut(s) 235, 364, 454, 592, 638, 944, 993, 1015
NgoMIV GCCGGC 1 cut(s) 36
NlaIII CATG 8 cut(s) 34, 182, 365, 415, 581, 671, 915, 1022
NlaIV GGNNCC 4 cut(s) 315, 606, 640, 953
NsiI ATGCAT 1 cut(s) 583
NspI RCATGY 1 cut(s) 671
PaeR7I CTCGAG 1 cut(s) 872
PagI TCATGA 2 cut(s) 178, 361
PciSI GCTCTTC 1 cut(s) 886
PcsI WCGNNNNNNNCGW 1 cut(s) 1032
PctI GAATGC 2 cut(s) 384, 770
PdiI GCCGGC 1 cut(s) 38
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 2 cut(s) 404, 916
PkrI GCNGC 4 cut(s) 286, 735, 1058, 1117
PleI GAGTC 2 cut(s) 864, 1039
PpsI GAGTC 2 cut(s) 864, 1039
Psp6I CCWGG 2 cut(s) 316, 736
PspGI CCWGG 2 cut(s) 316, 736
PspN4I GGNNCC 4 cut(s) 315, 606, 640, 953
PspPI GGNCC 5 cut(s) 130, 197, 983, 1039, 1148
PspXI VCTCGAGB 1 cut(s) 872
PsuI RGATCY 3 cut(s) 235, 638, 944
PvuII CAGCTG 1 cut(s) 492
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
SapI GCTCTTC 1 cut(s) 886
SaqAI TTAA 2 cut(s) 746, 1005
SatI GCNGC 4 cut(s) 285, 734, 1057, 1116
Sau3AI GATC 8 cut(s) 235, 364, 454, 592, 638, 944, 993, 1015
Sau96I GGNCC 5 cut(s) 130, 197, 983, 1039, 1148
SchI GAGTC 2 cut(s) 864, 1040
ScrFI CCNGG 3 cut(s) 233, 318, 738
SduI GDGCHC 1 cut(s) 898
SfaNI GCATC 5 cut(s) 35, 87, 158, 271, 967
Sfr274I CTCGAG 1 cut(s) 872
SinI GGWCC 2 cut(s) 130, 1148
SlaI CTCGAG 1 cut(s) 872
SmlI CTYRAG 1 cut(s) 872
SmoI CTYRAG 1 cut(s) 872
Sse9I AATT 6 cut(s) 289, 775, 820, 1002, 1063, 1100
SsiI CCGC 7 cut(s) 168, 229, 284, 734, 926, 1057, 1123
StyD4I CCNGG 3 cut(s) 231, 316, 736
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 372, 697
TaqI TCGA 7 cut(s) 296, 367, 407, 570, 780, 873, 1014
TasI AATT 6 cut(s) 289, 775, 820, 1002, 1063, 1100
TauI GCSGC 3 cut(s) 287, 736, 1059
TfiI GAWTC 2 cut(s) 404, 916
Tru1I TTAA 2 cut(s) 746, 1005
Tru9I TTAA 2 cut(s) 746, 1005
TscAI CASTG 2 cut(s) 650, 903
TseI GCWGC 1 cut(s) 1115
TspDTI ATGAA 5 cut(s) 55, 167, 348, 350, 849
TspRI CASTG 2 cut(s) 650, 903
VpaK11BI GGWCC 2 cut(s) 130, 1148
XapI RAATTY 3 cut(s) 289, 1063, 1100
XceI RCATGY 1 cut(s) 671
XcmI CCANNNNNNNNNTGG 1 cut(s) 314
XhoI CTCGAG 1 cut(s) 872
XmiI GTMKAC 2 cut(s) 558, 1023
XmnI GAANNNNTTC 1 cut(s) 263
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.