RLG00000031028

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002602
Physical Location & Seq
Forward (+)
1469471 .. 1472555
3085 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031028

Sequence Viewer

Length: 1188 bp
ATGGTGACGATGACGAAGAAGACGTCGCCACCGCCAATCACGGCGCGCCACGTGATCAGGTCCAGCTGGAAGCTTCTGATCGTCTTCTCCGTCTTGCTCTGCGTTTTGGCTCTGTACAGGTTTCAGTCTCAGCAGCCAGACCTTTACTCTCCTTCTTCTTCTTCTTCTTCTTCTTCATTATCTCGCGCGAGATCTCGAATTGCTCGCCACAGCGTCAGCTTCGCCGGCCCTGCCAAGATCGCCTTCCTATTTCTCGCGCGACGTGACCTCCCGCTCGATTTTCTTTGGGAGAGCTTCTTCGAGAATGCCGGTGGCGCTTCGAATTTTTCGATTTATATTCACTCGGCGCCTGGTTTTGTGTTCGATGAGTCCACGACGAGGTCTCGCTTCTTTCACGGCCGTCAGTTGCCGAATAGCATACAGGTGGGATGGGGAGAGTCGAGCATGATTGAAGCGGAGAGGTTGTTGTTTGCAACAGCTCTTGAGGATCCAGCAAATCAAAGATTTGTTCTCCTCTCTGACAGTTGCGTTCCTTTGTACAACTTCAGCTTCATATACAATTACCTGATGGCTTCTTCTAGGAGTATTGTTGACAGCTTCCTGGATGTGAAGGAGGGCCGCTACAACCCAAAAATGTCACCTATAATACCAAAAGCCAAATGGCGGAAAGGATCACAGTGGATTGCTTTGATTCGGACACACGCTGAAGTCCTTGTGGATGATGAAGTTATACTACCAGTTTTCAAAAAGTTTTGCAGACGTCGTCCACCTGTTGATGTCAGAAAGGGGAAGCTGAATCTTAAACTTCAGAAACAACACAACTGTATCCCAGACGAACACTATGTGCAGACACTGCTTGCGATGAGTGAGCTTGACGGCGAACTTGAGCGAAGGACATTAACCTATACATTGTGGAGCAACTCTCCAAATAAAACAGAGAGTAAAGGCTGGCATCCTATGACTTTCAACCATGCCAATGCGGCCCCTCAAAAGATTAGGGAAATAAAGGGGATCAACCATGTATACTATGAGACAGAATTCCGAACAGAATGGTGTCGTATAAATTCTACTGTTGTTCCCTGTTTTTTATTCGCTAGGAAGTTCTCCAAGGGTGCTGCCATGCGCCTTCTGAGCGAGGGAGTTGTTGGTCACTTTGACACAGCTGCATTGTTAAAACCATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

396

Amino Acids

45.02

Weight (kDa)

9.69

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 80 - 335 8.4e-69 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 26, 763
AccB1I GGYRCC 1 cut(s) 346
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 1023
AccII CGCG 5 cut(s) 46, 186, 188, 257, 259
AciI CCGC 6 cut(s) 32, 272, 455, 619, 664, 980
AclWI GGATC 4 cut(s) 482, 495, 679, 1019
AcoI YGGCCR 1 cut(s) 397
AcsI RAATTY 3 cut(s) 322, 1037, 1063
AcuI CTGAAG 3 cut(s) 529, 726, 791
AcvI CACGTG 1 cut(s) 52
AcyI GRCGYC 3 cut(s) 23, 347, 760
AdeI CACNNNGTG 1 cut(s) 844
AfaI GTAC 2 cut(s) 116, 539
AfiI CCNNNNNNNGG 2 cut(s) 378, 663
AgsI TTSAA 3 cut(s) 452, 745, 967
AjiI CACGTC 1 cut(s) 263
AjnI CCWGG 2 cut(s) 349, 600
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
Alw26I GTCTC 3 cut(s) 132, 387, 1025
AlwI GGATC 4 cut(s) 482, 495, 679, 1019
AlwNI CAGNNNCTG 1 cut(s) 853
AoxI GGCC 4 cut(s) 226, 397, 616, 981
ApeKI GCWGC 3 cut(s) 133, 1115, 1163
ApoI RAATTY 3 cut(s) 322, 1037, 1063
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AscI GGCGCGCC 1 cut(s) 44
AspLEI GCGC 7 cut(s) 46, 48, 188, 259, 317, 349, 1125
AspS9I GGNCC 4 cut(s) 60, 227, 616, 982
AsuHPI GGTGA 3 cut(s) 16, 630, 1173
AsuII TTCGAA 1 cut(s) 320
AvaII GGWCC 1 cut(s) 60
BaeI ACNNNNGTAYC 2 cut(s) 808, 841
BamHI GGATCC 1 cut(s) 487
BanI GGYRCC 1 cut(s) 346
BarI GAAGNNNNNNTAC 2 cut(s) 717, 749
BbrPI CACGTG 1 cut(s) 52
BbsI GAAGAC 2 cut(s) 26, 76
BbvI GCAGC 3 cut(s) 145, 1102, 1150
BccI CCATC 3 cut(s) 423, 562, 1186
BceAI ACGGC 4 cut(s) 57, 384, 412, 892
BciT130I CCWGG 2 cut(s) 351, 602
BciVI GTATCC 1 cut(s) 836
BclI TGATCA 1 cut(s) 54
BcoDI GTCTC 3 cut(s) 132, 387, 1025
BfaI CTAG 2 cut(s) 579, 1095
BfoI RGCGCY 2 cut(s) 318, 350
BfuI GTATCC 1 cut(s) 836
BglI GCCNNNNNGGC 1 cut(s) 980
BglII AGATCT 1 cut(s) 191
BisI GCNGC 5 cut(s) 134, 619, 981, 1116, 1164
BlsI GCNGC 5 cut(s) 135, 620, 982, 1117, 1165
Bme1390I CCNGG 2 cut(s) 351, 602
Bme18I GGWCC 1 cut(s) 60
BmgBI CACGTC 1 cut(s) 263
BmgT120I GGNCC 4 cut(s) 60, 227, 616, 982
BmiI GGNNCC 3 cut(s) 348, 489, 984
BmrFI CCNGG 2 cut(s) 351, 602
BmsI GCATC 1 cut(s) 961
BpiI GAAGAC 2 cut(s) 26, 76
BplI GAGNNNNNCTC 2 cut(s) 907, 939
Bpu14I TTCGAA 1 cut(s) 320
BpuEI CTTGAG 2 cut(s) 503, 905
BsaAI YACGTR 1 cut(s) 52
BsaHI GRCGYC 3 cut(s) 23, 347, 760
BsaI GGTCTC 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 1107
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 449, 479
Bsc4I CCNNNNNNNGG 2 cut(s) 378, 663
Bse118I RCCGGY 2 cut(s) 224, 308
Bse1I ACTGG 1 cut(s) 737
BseBI CCWGG 2 cut(s) 351, 602
BseDI CCNNGG 1 cut(s) 1107
BseGI GGATG 4 cut(s) 434, 610, 724, 952
BseLI CCNNNNNNNGG 2 cut(s) 378, 663
BseMII CTCAG 2 cut(s) 143, 1121
BseNI ACTGG 1 cut(s) 737
BsePI GCGCGC 1 cut(s) 44
BseRI GAGGAG 1 cut(s) 503
BseX3I CGGCCG 1 cut(s) 397
BseXI GCAGC 3 cut(s) 145, 1102, 1150
BsgI GTGCAG 1 cut(s) 866
Bsh1236I CGCG 5 cut(s) 46, 186, 188, 257, 259
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 4 cut(s) 228, 399, 618, 983
BshNI GGYRCC 1 cut(s) 346
BsiEI CGRYCG 1 cut(s) 400
BsiSI CCGG 2 cut(s) 225, 309
BslI CCNNNNNNNGG 2 cut(s) 378, 663
BsmAI GTCTC 3 cut(s) 132, 387, 1025
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 4 cut(s) 228, 399, 618, 983
Bso31I GGTCTC 1 cut(s) 387
Bsp119I TTCGAA 1 cut(s) 320
Bsp1407I TGTACA 2 cut(s) 114, 537
Bsp143I GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
BspACI CCGC 6 cut(s) 32, 272, 455, 619, 664, 980
BspANI GGCC 4 cut(s) 228, 399, 618, 983
BspCNI CTCAG 2 cut(s) 142, 1122
BspFNI CGCG 5 cut(s) 46, 186, 188, 257, 259
BspLI GGNNCC 3 cut(s) 348, 489, 984
BspPI GGATC 4 cut(s) 482, 495, 679, 1019
BspT104I TTCGAA 1 cut(s) 320
BspT107I GGYRCC 1 cut(s) 346
BspTNI GGTCTC 1 cut(s) 387
BsrBI CCGCTC 1 cut(s) 274
BsrFI RCCGGY 2 cut(s) 224, 308
BsrGI TGTACA 2 cut(s) 114, 537
BsrI ACTGG 1 cut(s) 737
BssAI RCCGGY 2 cut(s) 224, 308
BssECI CCNNGG 1 cut(s) 1107
BssHII GCGCGC 1 cut(s) 44
BssMI GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
BssNAI GTATAC 1 cut(s) 1024
BssNI GRCGYC 3 cut(s) 23, 347, 760
BssT1I CCWWGG 1 cut(s) 1107
Bst1107I GTATAC 1 cut(s) 1024
Bst2UI CCWGG 2 cut(s) 351, 602
Bst4CI ACNGT 4 cut(s) 524, 678, 824, 1072
BstACI GRCGYC 3 cut(s) 23, 347, 760
BstAPI GCANNNNNTGC 1 cut(s) 853
BstAUI TGTACA 2 cut(s) 114, 537
BstBAI YACGTR 1 cut(s) 52
BstBI TTCGAA 1 cut(s) 320
BstC8I GCNNGC 5 cut(s) 46, 205, 226, 858, 950
BstDEI CTNAG 2 cut(s) 129, 1130
BstF5I GGATG 4 cut(s) 434, 610, 724, 952
BstFNI CGCG 5 cut(s) 46, 186, 188, 257, 259
BstH2I RGCGCY 2 cut(s) 318, 350
BstHHI GCGC 7 cut(s) 46, 48, 188, 259, 317, 349, 1125
BstKTI GATC 7 cut(s) 57, 81, 194, 240, 490, 674, 1014
BstMAI GTCTC 3 cut(s) 132, 387, 1025
BstMBI GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 7 cut(s) 225, 230, 239, 314, 853, 980, 1131
BstNI CCWGG 2 cut(s) 351, 602
BstSCI CCNGG 2 cut(s) 349, 600
BstUI CGCG 5 cut(s) 46, 186, 188, 257, 259
BstV1I GCAGC 3 cut(s) 145, 1102, 1150
BstV2I GAAGAC 2 cut(s) 26, 76
BstX2I RGATCY 2 cut(s) 191, 487
BstYI RGATCY 2 cut(s) 191, 487
BstZ17I GTATAC 1 cut(s) 1024
BstZI CGGCCG 1 cut(s) 397
BsuI GTATCC 1 cut(s) 836
BsuRI GGCC 4 cut(s) 228, 399, 618, 983
BtgZI GCGATG 1 cut(s) 875
BtrI CACGTC 1 cut(s) 263
BtsCI GGATG 4 cut(s) 434, 610, 724, 952
BtsI GCAGTG 1 cut(s) 851
BtsIMutI CAGTG 2 cut(s) 683, 851
Cac8I GCNNGC 5 cut(s) 46, 205, 226, 858, 950
CaiI CAGNNNCTG 1 cut(s) 853
CfoI GCGC 7 cut(s) 46, 48, 188, 259, 317, 349, 1125
Cfr10I RCCGGY 2 cut(s) 224, 308
Cfr13I GGNCC 4 cut(s) 60, 227, 616, 982
CseI GACGC 1 cut(s) 202
Csp6I GTAC 2 cut(s) 115, 538
CviAII CATG 5 cut(s) 445, 971, 1019, 1120, 1185
CviQI GTAC 2 cut(s) 115, 538
DdeI CTNAG 2 cut(s) 129, 1130
DinI GGCGCC 1 cut(s) 348
DpnI GATC 7 cut(s) 56, 80, 193, 239, 489, 673, 1013
DpnII GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
DraIII CACNNNGTG 1 cut(s) 844
EaeI YGGCCR 1 cut(s) 397
EagI CGGCCG 1 cut(s) 397
EciI GGCGGA 1 cut(s) 679
EclXI CGGCCG 1 cut(s) 397
Eco130I CCWWGG 1 cut(s) 1107
Eco31I GGTCTC 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 60
Eco52I CGGCCG 1 cut(s) 397
Eco57I CTGAAG 3 cut(s) 529, 726, 791
Eco72I CACGTG 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 1037
EcoRII CCWGG 2 cut(s) 349, 600
EcoT14I CCWWGG 1 cut(s) 1107
EgeI GGCGCC 1 cut(s) 348
EheI GGCGCC 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 1107
FaeI CATG 5 cut(s) 448, 974, 1022, 1123, 1188
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FatI CATG 5 cut(s) 444, 970, 1018, 1119, 1184
FauI CCCGC 1 cut(s) 279
FbaI TGATCA 1 cut(s) 54
FblI GTMKAC 1 cut(s) 1023
Fnu4HI GCNGC 5 cut(s) 134, 619, 981, 1116, 1164
FokI GGATG 4 cut(s) 441, 617, 731, 939
Fsp4HI GCNGC 5 cut(s) 134, 619, 981, 1116, 1164
FspBI CTAG 2 cut(s) 579, 1095
GlaI GCGC 7 cut(s) 45, 47, 187, 258, 316, 348, 1124
GluI GCNGC 5 cut(s) 134, 619, 981, 1116, 1164
HaeII RGCGCY 2 cut(s) 318, 350
HaeIII GGCC 4 cut(s) 228, 399, 618, 983
HapII CCGG 2 cut(s) 225, 309
HgaI GACGC 1 cut(s) 202
HhaI GCGC 7 cut(s) 46, 48, 188, 259, 317, 349, 1125
Hin1I GRCGYC 3 cut(s) 23, 347, 760
Hin1II CATG 5 cut(s) 448, 974, 1022, 1123, 1188
Hin6I GCGC 7 cut(s) 44, 46, 186, 257, 315, 347, 1123
HinP1I GCGC 7 cut(s) 44, 46, 186, 257, 315, 347, 1123
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 4 cut(s) 368, 437, 691, 796
HpaII CCGG 2 cut(s) 225, 309
HphI GGTGA 3 cut(s) 16, 630, 1173
Hpy166II GTNNAC 4 cut(s) 372, 592, 767, 1024
Hpy188I TCNGA 7 cut(s) 78, 520, 696, 782, 810, 1043, 1131
Hpy188III TCNNGA 3 cut(s) 195, 301, 482
Hpy8I GTNNAC 4 cut(s) 372, 592, 767, 1024
Hpy99I CGWCG 4 cut(s) 28, 264, 379, 765
HpyAV CCTTC 5 cut(s) 162, 253, 604, 885, 1136
HpyCH4III ACNGT 4 cut(s) 524, 678, 824, 1072
HpyCH4IV ACGT 4 cut(s) 23, 51, 262, 760
HpyCH4V TGCA 4 cut(s) 473, 756, 847, 1166
HpyF10VI GCNNNNNNNGC 7 cut(s) 225, 230, 239, 314, 853, 980, 1131
HpyF3I CTNAG 2 cut(s) 129, 1130
HpySE526I ACGT 4 cut(s) 23, 51, 262, 760
Hsp92I GRCGYC 3 cut(s) 23, 347, 760
Hsp92II CATG 5 cut(s) 448, 974, 1022, 1123, 1188
HspAI GCGC 7 cut(s) 44, 46, 186, 257, 315, 347, 1123
KasI GGCGCC 1 cut(s) 346
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
LmnI GCTCC 1 cut(s) 915
Lsp1109I GCAGC 3 cut(s) 145, 1102, 1150
LweI GCATC 1 cut(s) 961
MaeI CTAG 2 cut(s) 579, 1095
MaeII ACGT 4 cut(s) 23, 51, 262, 760
MaeIII GTNAC 4 cut(s) 4, 263, 636, 1148
MalI GATC 7 cut(s) 56, 80, 193, 239, 489, 673, 1013
MbiI CCGCTC 1 cut(s) 274
MboI GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
MflI RGATCY 2 cut(s) 191, 487
MluCI AATT 5 cut(s) 198, 322, 559, 1037, 1063
Mly113I GGCGCC 1 cut(s) 347
MlyI GAGTC 2 cut(s) 377, 446
MnlI CCTC 8 cut(s) 278, 372, 453, 478, 524, 607, 996, 1129
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 3 cut(s) 801, 899, 1172
MslI CAYNNNNRTG 3 cut(s) 422, 975, 1183
MspA1I CMGCKG 2 cut(s) 66, 1163
MspI CCGG 2 cut(s) 225, 309
MspR9I CCNGG 2 cut(s) 351, 602
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 2 cut(s) 351, 602
MvnI CGCG 5 cut(s) 46, 186, 188, 257, 259
MwoI GCNNNNNNNGC 7 cut(s) 225, 230, 239, 314, 853, 980, 1131
NaeI GCCGGC 1 cut(s) 226
NarI GGCGCC 1 cut(s) 347
NdeII GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 5 cut(s) 448, 974, 1022, 1123, 1188
NlaIV GGNNCC 3 cut(s) 348, 489, 984
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 4 cut(s) 4, 263, 636, 1148
NspV TTCGAA 1 cut(s) 320
PalAI GGCGCGCC 1 cut(s) 44
PauI GCGCGC 1 cut(s) 44
PcsI WCGNNNNNNNCGW 3 cut(s) 20, 87, 326
PctI GAATGC 1 cut(s) 310
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 2 cut(s) 691, 796
PflFI GACNNNGTC 2 cut(s) 379, 762
PfoI TCCNGGA 1 cut(s) 600
PkrI GCNGC 5 cut(s) 135, 620, 982, 1117, 1165
PleI GAGTC 2 cut(s) 376, 445
PluTI GGCGCC 1 cut(s) 350
PmaCI CACGTG 1 cut(s) 52
PmlI CACGTG 1 cut(s) 52
PpsI GAGTC 2 cut(s) 376, 445
Ppu21I YACGTR 1 cut(s) 52
Psp6I CCWGG 2 cut(s) 349, 600
PspCI CACGTG 1 cut(s) 52
PspGI CCWGG 2 cut(s) 349, 600
PspN4I GGNNCC 3 cut(s) 348, 489, 984
PspPI GGNCC 4 cut(s) 60, 227, 616, 982
PstNI CAGNNNCTG 1 cut(s) 853
PsuI RGATCY 2 cut(s) 191, 487
PsyI GACNNNGTC 2 cut(s) 379, 762
PteI GCGCGC 1 cut(s) 44
PvuII CAGCTG 2 cut(s) 66, 1163
RsaI GTAC 2 cut(s) 116, 539
RsaNI GTAC 2 cut(s) 115, 538
RseI CAYNNNNRTG 3 cut(s) 422, 975, 1183
SaqAI TTAA 3 cut(s) 801, 899, 1172
SatI GCNGC 5 cut(s) 134, 619, 981, 1116, 1164
Sau3AI GATC 7 cut(s) 54, 78, 191, 237, 487, 671, 1011
Sau96I GGNCC 4 cut(s) 60, 227, 616, 982
SchI GAGTC 2 cut(s) 377, 446
ScrFI CCNGG 2 cut(s) 351, 602
SfaNI GCATC 1 cut(s) 961
SfoI GGCGCC 1 cut(s) 348
SfuI TTCGAA 1 cut(s) 320
SgsI GGCGCGCC 1 cut(s) 44
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 3 cut(s) 422, 975, 1183
SmlI CTYRAG 2 cut(s) 482, 884
SmoI CTYRAG 2 cut(s) 482, 884
Sse9I AATT 5 cut(s) 198, 322, 559, 1037, 1063
SsiI CCGC 6 cut(s) 32, 272, 455, 619, 664, 980
SspDI GGCGCC 1 cut(s) 346
SspMI CTAG 2 cut(s) 579, 1095
StyD4I CCNGG 2 cut(s) 349, 600
StyI CCWWGG 1 cut(s) 1107
TaaI ACNGT 4 cut(s) 524, 678, 824, 1072
TaiI ACGT 4 cut(s) 26, 54, 265, 763
TaqI TCGA 7 cut(s) 196, 276, 300, 320, 329, 363, 440
TasI AATT 5 cut(s) 198, 322, 559, 1037, 1063
TatI WGTACW 2 cut(s) 114, 537
TauI GCSGC 2 cut(s) 621, 983
TfiI GAWTC 2 cut(s) 691, 796
Tru1I TTAA 3 cut(s) 801, 899, 1172
Tru9I TTAA 3 cut(s) 801, 899, 1172
TscAI CASTG 2 cut(s) 683, 858
TseFI GTSAC 4 cut(s) 4, 263, 636, 1148
TseI GCWGC 3 cut(s) 133, 1115, 1163
Tsp45I GTSAC 4 cut(s) 4, 263, 636, 1148
TspDTI ATGAA 3 cut(s) 165, 541, 738
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 2 cut(s) 683, 858
Tth111I GACNNNGTC 2 cut(s) 379, 762
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 3 cut(s) 322, 1037, 1063
XcmI CCANNNNNNNNNTGG 1 cut(s) 657
XmiI GTMKAC 1 cut(s) 1023
XspI CTAG 2 cut(s) 579, 1095
ZraI GACGTC 2 cut(s) 24, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.