Prupe.4G016500_v2.0.a1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0002602
Physical Location & Seq
Forward (+)
790703 .. 794480
3778 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G016500.2

Sequence Viewer

Length: 1158 bp
ATGATGATGACGAAGAAGTCACCACCAATCCCGGCGCGCCACGTACTCAGGTTCAGCTGGCAGCTGGTGGTCATCCTCTCGATCACTCTCTGCGTCTTAGCCTTCTTCAAGCTTCACTCGCAACCTGACCTCTACTCCTCTCCTTCTTCATTATCTATCGCAAGATCTCGAGTTTCTCGCCATGGCAACAACTTTAGCGGCCCTCCCAAGATTGCCTTCCTTTTTCTCGCCCGCCGTAGCCTCCCCCTCGATTTTCTTTGGGGCAGCTTCTTCGAGAGTGCTGACATGCCGAATTTTTCGATTTATATTCACTCGGCGCCTGGTTTTTCGTTCGACGAGTCGACGACGAGATCACATTTCTTTTACGGCCGCCAATTGACGAATAGCATTCAGGTGGGTTGGGGAGAATCAAGTATGATCGAAGCAGAGAGGTTGTTGTTCGCAACAGCTCTTGAGGATCCAGCGAATCAACGATTTGTACTCCTCTCTGACAGTTGTGTTCCTTTGTACAACTTCAGCTACATATACAACTATTTGATGGCTTCTCCAAGGAGCTTTGTGGACAGCTTTCTTGATGTAAAGGAGGGCCGCTACAACCCGAAAATGTCACCTAACATACCAAAACAAAAATGGCGAAAAGGATCCCAGTGGATTGCTTTGGTTCGAAGCCATGCTGAAGTCCTTGTGGATGATGAAGTTGTACTACCAGCTTTCAGGAAATTTTGCAAGCGTCGCCCACCTCTGGATGCCAGAAAGGGGAAGCTGAACATTAAACTTCAGAAGCAACACAACTGTATCCCAGATGAACACTATGTGCAGACACTGCTTACGATGAGTGAGCGTGAAGACGAACTTGAGCGAAGGACATTAACCTATACACTGTGGAACAACTCTGCAACTAAAACAGAGAGTAAAGGCTGGCATCCTATGACTTTCACCCACGCGAATGCAGCCCCTCACAAAATAAAGGAAATAAAGGAGATCAACCATGTATACTATGAGACAGAATACCGGACAGAGTGGTGTCGTGTAAATTCGACATATGTTCCCTGTTTTTTATTCGCAAGGAAATTCTCCCAGGGGGCTGCCATGCGCCTTCTTAGCGAGGGAGTGGTTGGTCAATTTGATACCTCTTCATTATTAGATCCACCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

386

Amino Acids

44.42

Weight (kDa)

9.31

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 316
AccI GTMKAC 2 cut(s) 341, 993
AccII CGCG 2 cut(s) 37, 944
AciI CCGC 4 cut(s) 198, 232, 370, 589
AclWI GGATC 5 cut(s) 452, 465, 636, 649, 1139
AcoI YGGCCR 1 cut(s) 367
AcsI RAATTY 4 cut(s) 292, 719, 1033, 1070
AcuI CTGAAG 3 cut(s) 499, 696, 761
AcyI GRCGYC 1 cut(s) 317
AdeI CACNNNGTG 1 cut(s) 814
AfaI GTAC 4 cut(s) 45, 480, 509, 702
AfiI CCNNNNNNNGG 2 cut(s) 742, 756
AgsI TTSAA 1 cut(s) 109
AjnI CCWGG 2 cut(s) 319, 1077
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
Alw26I GTCTC 1 cut(s) 995
AlwI GGATC 5 cut(s) 452, 465, 636, 649, 1139
AlwNI CAGNNNCTG 1 cut(s) 823
Ama87I CYCGRG 1 cut(s) 168
AoxI GGCC 3 cut(s) 199, 367, 586
ApeKI GCWGC 4 cut(s) 61, 264, 950, 1085
ApoI RAATTY 4 cut(s) 292, 719, 1033, 1070
AscI GGCGCGCC 1 cut(s) 35
AspLEI GCGC 4 cut(s) 37, 39, 319, 1095
AspS9I GGNCC 2 cut(s) 200, 586
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 3 cut(s) 12, 600, 928
AsuII TTCGAA 1 cut(s) 664
AvaI CYCGRG 1 cut(s) 168
BaeI ACNNNNGTAYC 2 cut(s) 778, 811
BamHI GGATCC 2 cut(s) 457, 641
BanI GGYRCC 1 cut(s) 316
BarI GAAGNNNNNNTAC 2 cut(s) 687, 719
BbsI GAAGAC 1 cut(s) 852
BbvI GCAGC 4 cut(s) 73, 276, 962, 1072
BccI CCATC 1 cut(s) 532
BceAI ACGGC 2 cut(s) 219, 382
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 2 cut(s) 321, 1079
BciVI GTATCC 1 cut(s) 806
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 995
BfoI RGCGCY 1 cut(s) 320
BfuI GTATCC 1 cut(s) 806
BglII AGATCT 1 cut(s) 164
BisI GCNGC 7 cut(s) 62, 199, 265, 370, 589, 951, 1086
BlsI GCNGC 7 cut(s) 63, 200, 266, 371, 590, 952, 1087
Bme1390I CCNGG 3 cut(s) 32, 321, 1079
BmeT110I CYCGRG 1 cut(s) 168
BmgT120I GGNCC 2 cut(s) 200, 586
BmiI GGNNCC 3 cut(s) 318, 459, 643
BmrFI CCNGG 3 cut(s) 32, 321, 1079
BmrI ACTGGG 1 cut(s) 640
BmsI GCATC 2 cut(s) 736, 931
BmuI ACTGGG 1 cut(s) 640
BpiI GAAGAC 1 cut(s) 852
Bpu14I TTCGAA 1 cut(s) 664
BpuEI CTTGAG 2 cut(s) 473, 875
BpuMI CCSGG 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 4 cut(s) 181, 548, 1077, 1078
BsaWI WCCGGW 1 cut(s) 1011
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 2 cut(s) 742, 756
Bse1I ACTGG 1 cut(s) 646
BseBI CCWGG 2 cut(s) 321, 1079
BseDI CCNNGG 4 cut(s) 181, 548, 1077, 1078
BseGI GGATG 4 cut(s) 72, 694, 751, 922
BseLI CCNNNNNNNGG 2 cut(s) 742, 756
BseMII CTCAG 1 cut(s) 61
BseNI ACTGG 1 cut(s) 646
BsePI GCGCGC 1 cut(s) 35
BseRI GAGGAG 2 cut(s) 127, 473
BseX3I CGGCCG 1 cut(s) 367
BseXI GCAGC 4 cut(s) 73, 276, 962, 1072
BsgI GTGCAG 1 cut(s) 836
Bsh1236I CGCG 2 cut(s) 37, 944
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 3 cut(s) 201, 369, 588
BshNI GGYRCC 1 cut(s) 316
BsiEI CGRYCG 1 cut(s) 370
BsiHKCI CYCGRG 1 cut(s) 168
BsiSI CCGG 2 cut(s) 32, 1012
BslI CCNNNNNNNGG 2 cut(s) 742, 756
BsmAI GTCTC 1 cut(s) 995
BsmI GAATGC 2 cut(s) 387, 952
BsnI GGCC 3 cut(s) 201, 369, 588
BsoBI CYCGRG 1 cut(s) 168
Bsp119I TTCGAA 1 cut(s) 664
Bsp1407I TGTACA 1 cut(s) 507
Bsp143I GATC 8 cut(s) 81, 164, 350, 417, 457, 641, 981, 1144
Bsp19I CCATGG 1 cut(s) 181
BspACI CCGC 4 cut(s) 198, 232, 370, 589
BspANI GGCC 3 cut(s) 201, 369, 588
BspCNI CTCAG 1 cut(s) 60
BspFNI CGCG 2 cut(s) 37, 944
BspLI GGNNCC 3 cut(s) 318, 459, 643
BspPI GGATC 5 cut(s) 452, 465, 636, 649, 1139
BspT104I TTCGAA 1 cut(s) 664
BspT107I GGYRCC 1 cut(s) 316
BsrGI TGTACA 1 cut(s) 507
BsrI ACTGG 1 cut(s) 646
BssECI CCNNGG 4 cut(s) 181, 548, 1077, 1078
BssHII GCGCGC 1 cut(s) 35
BssMI GATC 8 cut(s) 81, 164, 350, 417, 457, 641, 981, 1144
BssNAI GTATAC 1 cut(s) 994
BssNI GRCGYC 1 cut(s) 317
BssT1I CCWWGG 2 cut(s) 181, 548
Bst1107I GTATAC 1 cut(s) 994
Bst2UI CCWGG 2 cut(s) 321, 1079
Bst4CI ACNGT 3 cut(s) 494, 794, 882
Bst6I CTCTTC 1 cut(s) 1138
BstACI GRCGYC 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 823
BstAUI TGTACA 1 cut(s) 507
BstBAI YACGTR 1 cut(s) 43
BstBI TTCGAA 1 cut(s) 664
BstC8I GCNNGC 5 cut(s) 37, 59, 232, 728, 920
BstDEI CTNAG 3 cut(s) 47, 97, 1100
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 4 cut(s) 72, 694, 751, 922
BstFNI CGCG 2 cut(s) 37, 944
BstH2I RGCGCY 1 cut(s) 320
BstHHI GCGC 4 cut(s) 37, 39, 319, 1095
BstKTI GATC 8 cut(s) 84, 167, 353, 420, 460, 644, 984, 1147
BstMAI GTCTC 1 cut(s) 995
BstMBI GATC 8 cut(s) 81, 164, 350, 417, 457, 641, 981, 1144
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 5 cut(s) 118, 732, 823, 950, 1101
BstNI CCWGG 2 cut(s) 321, 1079
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 3 cut(s) 30, 319, 1077
BstUI CGCG 2 cut(s) 37, 944
BstV1I GCAGC 4 cut(s) 73, 276, 962, 1072
BstV2I GAAGAC 1 cut(s) 852
BstX2I RGATCY 4 cut(s) 164, 457, 641, 1144
BstYI RGATCY 4 cut(s) 164, 457, 641, 1144
BstZ17I GTATAC 1 cut(s) 994
BstZI CGGCCG 1 cut(s) 367
BsuI GTATCC 1 cut(s) 806
BsuRI GGCC 3 cut(s) 201, 369, 588
BtgI CCRYGG 1 cut(s) 181
BtsCI GGATG 4 cut(s) 72, 694, 751, 922
BtsI GCAGTG 1 cut(s) 821
BtsIMutI CAGTG 3 cut(s) 653, 821, 878
Cac8I GCNNGC 5 cut(s) 37, 59, 232, 728, 920
CaiI CAGNNNCTG 1 cut(s) 823
CfoI GCGC 4 cut(s) 37, 39, 319, 1095
Cfr13I GGNCC 2 cut(s) 200, 586
CseI GACGC 2 cut(s) 82, 719
Csp6I GTAC 4 cut(s) 44, 479, 508, 701
CviAII CATG 6 cut(s) 182, 286, 671, 989, 1090, 1155
CviQI GTAC 4 cut(s) 44, 479, 508, 701
DdeI CTNAG 3 cut(s) 47, 97, 1100
DinI GGCGCC 1 cut(s) 318
DpnI GATC 8 cut(s) 83, 166, 352, 419, 459, 643, 983, 1146
DpnII GATC 8 cut(s) 81, 164, 350, 417, 457, 641, 981, 1144
DraIII CACNNNGTG 1 cut(s) 814
DrdI GACNNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 16
EaeI YGGCCR 1 cut(s) 367
EagI CGGCCG 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 1138
EarI CTCTTC 1 cut(s) 1138
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 2 cut(s) 181, 548
Eco52I CGGCCG 1 cut(s) 367
Eco57I CTGAAG 3 cut(s) 499, 696, 761
Eco88I CYCGRG 1 cut(s) 168
EcoRII CCWGG 2 cut(s) 319, 1077
EcoT14I CCWWGG 2 cut(s) 181, 548
EgeI GGCGCC 1 cut(s) 318
EheI GGCGCC 1 cut(s) 318
ErhI CCWWGG 2 cut(s) 181, 548
FaeI CATG 6 cut(s) 185, 289, 674, 992, 1093, 1158
FalI AAGNNNNNCTT 4 cut(s) 200, 232, 837, 869
FatI CATG 6 cut(s) 181, 285, 670, 988, 1089, 1154
FauI CCCGC 1 cut(s) 239
FauNDI CATATG 1 cut(s) 1042
FblI GTMKAC 2 cut(s) 341, 993
Fnu4HI GCNGC 7 cut(s) 62, 199, 265, 370, 589, 951, 1086
FokI GGATG 4 cut(s) 59, 701, 758, 909
Fsp4HI GCNGC 7 cut(s) 62, 199, 265, 370, 589, 951, 1086
GlaI GCGC 4 cut(s) 36, 38, 318, 1094
GluI GCNGC 7 cut(s) 62, 199, 265, 370, 589, 951, 1086
HaeII RGCGCY 1 cut(s) 320
HaeIII GGCC 3 cut(s) 201, 369, 588
HapII CCGG 2 cut(s) 32, 1012
HgaI GACGC 2 cut(s) 82, 719
HhaI GCGC 4 cut(s) 37, 39, 319, 1095
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 6 cut(s) 185, 289, 674, 992, 1093, 1158
Hin6I GCGC 4 cut(s) 35, 37, 317, 1093
HinP1I GCGC 4 cut(s) 35, 37, 317, 1093
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 3 cut(s) 338, 407, 466
HpaII CCGG 2 cut(s) 32, 1012
HphI GGTGA 3 cut(s) 12, 600, 928
Hpy166II GTNNAC 3 cut(s) 342, 562, 994
Hpy188I TCNGA 2 cut(s) 490, 780
Hpy188III TCNNGA 7 cut(s) 79, 168, 274, 452, 572, 715, 743
Hpy8I GTNNAC 3 cut(s) 342, 562, 994
Hpy99I CGWCG 4 cut(s) 338, 346, 349, 735
HpyAV CCTTC 5 cut(s) 112, 153, 226, 855, 1106
HpyCH4III ACNGT 3 cut(s) 494, 794, 882
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 4 cut(s) 726, 817, 896, 950
HpyF10VI GCNNNNNNNGC 5 cut(s) 118, 732, 823, 950, 1101
HpyF3I CTNAG 3 cut(s) 47, 97, 1100
HpySE526I ACGT 1 cut(s) 42
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 6 cut(s) 185, 289, 674, 992, 1093, 1158
HspAI GCGC 4 cut(s) 35, 37, 317, 1093
KasI GGCGCC 1 cut(s) 316
Kzo9I GATC 8 cut(s) 81, 164, 350, 417, 457, 641, 981, 1144
LmnI GCTCC 1 cut(s) 552
Lsp1109I GCAGC 4 cut(s) 73, 276, 962, 1072
LweI GCATC 2 cut(s) 736, 931
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 2 cut(s) 18, 606
MalI GATC 8 cut(s) 83, 166, 352, 419, 459, 643, 983, 1146
MboI GATC 8 cut(s) 81, 164, 350, 417, 457, 641, 981, 1144
MboII GAAGA 6 cut(s) 25, 97, 138, 262, 857, 1125
MfeI CAATTG 1 cut(s) 374
MflI RGATCY 4 cut(s) 164, 457, 641, 1144
MluCI AATT 6 cut(s) 292, 374, 719, 1033, 1070, 1121
Mly113I GGCGCC 1 cut(s) 317
MlyI GAGTC 1 cut(s) 347
MseI TTAA 2 cut(s) 771, 869
MslI CAYNNNNRTG 3 cut(s) 392, 945, 1153
MspA1I CMGCKG 2 cut(s) 57, 64
MspI CCGG 2 cut(s) 32, 1012
MspR9I CCNGG 3 cut(s) 32, 321, 1079
MunI CAATTG 1 cut(s) 374
Mva1269I GAATGC 2 cut(s) 387, 952
MvaI CCWGG 2 cut(s) 321, 1079
MvnI CGCG 2 cut(s) 37, 944
MwoI GCNNNNNNNGC 5 cut(s) 118, 732, 823, 950, 1101
NarI GGCGCC 1 cut(s) 317
NciI CCSGG 1 cut(s) 32
NcoI CCATGG 1 cut(s) 181
NdeI CATATG 1 cut(s) 1042
NdeII GATC 8 cut(s) 81, 164, 350, 417, 457, 641, 981, 1144
NlaIII CATG 6 cut(s) 185, 289, 674, 992, 1093, 1158
NlaIV GGNNCC 3 cut(s) 318, 459, 643
NmeAIII GCCGAG 1 cut(s) 293
NmuCI GTSAC 2 cut(s) 18, 606
NspI RCATGY 1 cut(s) 289
NspV TTCGAA 1 cut(s) 664
PaeR7I CTCGAG 1 cut(s) 168
PalAI GGCGCGCC 1 cut(s) 35
PasI CCCWGGG 1 cut(s) 1078
PauI GCGCGC 1 cut(s) 35
PctI GAATGC 2 cut(s) 387, 952
PfeI GAWTC 2 cut(s) 407, 466
PkrI GCNGC 7 cut(s) 63, 200, 266, 371, 590, 952, 1087
PleI GAGTC 1 cut(s) 346
PluTI GGCGCC 1 cut(s) 320
PpsI GAGTC 1 cut(s) 346
Ppu21I YACGTR 1 cut(s) 43
Psp6I CCWGG 2 cut(s) 319, 1077
PspGI CCWGG 2 cut(s) 319, 1077
PspN4I GGNNCC 3 cut(s) 318, 459, 643
PspPI GGNCC 2 cut(s) 200, 586
PstNI CAGNNNCTG 1 cut(s) 823
PsuI RGATCY 4 cut(s) 164, 457, 641, 1144
PteI GCGCGC 1 cut(s) 35
PvuII CAGCTG 2 cut(s) 57, 64
RsaI GTAC 4 cut(s) 45, 480, 509, 702
RsaNI GTAC 4 cut(s) 44, 479, 508, 701
RseI CAYNNNNRTG 3 cut(s) 392, 945, 1153
SalI GTCGAC 1 cut(s) 340
SaqAI TTAA 2 cut(s) 771, 869
SatI GCNGC 7 cut(s) 62, 199, 265, 370, 589, 951, 1086
Sau3AI GATC 8 cut(s) 81, 164, 350, 417, 457, 641, 981, 1144
Sau96I GGNCC 2 cut(s) 200, 586
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 3 cut(s) 32, 321, 1079
SfaNI GCATC 2 cut(s) 736, 931
SfoI GGCGCC 1 cut(s) 318
Sfr274I CTCGAG 1 cut(s) 168
SfuI TTCGAA 1 cut(s) 664
SgsI GGCGCGCC 1 cut(s) 35
SlaI CTCGAG 1 cut(s) 168
SmiMI CAYNNNNRTG 3 cut(s) 392, 945, 1153
SmlI CTYRAG 3 cut(s) 168, 452, 854
SmoI CTYRAG 3 cut(s) 168, 452, 854
Sse9I AATT 6 cut(s) 292, 374, 719, 1033, 1070, 1121
SsiI CCGC 4 cut(s) 198, 232, 370, 589
SspDI GGCGCC 1 cut(s) 316
StyD4I CCNGG 3 cut(s) 30, 319, 1077
StyI CCWWGG 2 cut(s) 181, 548
TaaI ACNGT 3 cut(s) 494, 794, 882
TaiI ACGT 1 cut(s) 45
TasI AATT 6 cut(s) 292, 374, 719, 1033, 1070, 1121
TatI WGTACW 3 cut(s) 478, 507, 700
TauI GCSGC 3 cut(s) 201, 372, 591
TfiI GAWTC 2 cut(s) 407, 466
Tru1I TTAA 2 cut(s) 771, 869
Tru9I TTAA 2 cut(s) 771, 869
TscAI CASTG 3 cut(s) 653, 828, 885
TseFI GTSAC 2 cut(s) 18, 606
TseI GCWGC 4 cut(s) 61, 264, 950, 1085
Tsp45I GTSAC 2 cut(s) 18, 606
TspDTI ATGAA 4 cut(s) 138, 708, 819, 1125
TspRI CASTG 3 cut(s) 653, 828, 885
XapI RAATTY 4 cut(s) 292, 719, 1033, 1070
XceI RCATGY 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 627
XhoI CTCGAG 1 cut(s) 168
XmiI GTMKAC 2 cut(s) 341, 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.