RchiOBHm_Chr5g0002391

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002602
Physical Location & Seq
Forward (+)
1394614 .. 1398628
4015 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28377

Sequence Viewer

Length: 1176 bp
ATGGTGACGATGACGAAGAAGACGTCGCCACCGCCAATCACGGCGCGCCACGTGATCAGGTCCAGCTGGAAGCTTCTGATCGTCTTCTCCGTCTTGCTCTGCGTTTTGGCTCTGTACAGGTTTCAGTCTCAGCAGCCAGACCTTTACTCTCCTTCTTCTTCTTCATTATCTCGCGCGAGATCTCGAATTGCTCGCCACAGCGTCAGCTTCGCCGGCCCCGCCAAGATCGCCTTCCTATTTCTCGCGCGACGTGACGTCCCGCTCGATTTTCTTTGGGAGAGCTTCTTCGAGAATGCCGGTGGCGCTTTGAATTTTTCGATTTATATTCACTCGGCGCCTGGTTTTGTGTTCGATGAGTCCACGACGAGGTCTCGGTTCTTTCACGGCCGTCAGTTGCCGAATAGCATACAGGTGGGATGGGGAGAATCGAGCATGATTGAAGCGGAGAGGTTGTTGTTTGCAACAGCTCTTGAGGATCCAGCAAATCAAAGATTTGTTCTCCTCTCTGACAGTTGCGTTCCTTTGTACAACTTCAGCTTCATATACAATTACCTGATGACTTCTCGTAGGAGTATTGTTGACAGCTTCCTGGATGTGAAGGAGGGCCGCTACAACCCAAAAATGTCACCCGTAATACCAAAAGCCAAATGGCGGAAAGGATCACAGTGGATTGCTTTGATTCGGAGACACGCTGAAGTCCTTGTGGATGATGAAGTTATACTTCCAGTTTTCAAAAAGTTTTGCAGACGTCGTCCACCTGTTGATATCAGAAAGGGGAAGCTGAATCTTAAACTTCAGAAACAGCACAACTGTATCCCAGACGAACACTATGTGCAGACACTGCTTGCGATGAGTGAGCTTGATGGCGAACTTGAGCGAAGGACATTAACCTATACATTGTGGAGCAACTCTCCAAATAAAACAGAGAGTAAAGGCTGGCATCCTATGACTTTCAACCATGCCAATGCGGCCCCTCAAAAGATTAGGGAAATAAAGGGGATCAACCATGTATACTATGAGACAGAATTCCGAACAGAATGGTGTCGTATAAATTCTACTGTTGTTCCCTGTTTTTTATTCGCAAGGAAGTTCTCCAAGGGTGCTGCCATGCGCCTTCTGAGCGAGGGAGTTGTTGGTCACTTTGACACAGCTGCATTGTTAAAACCATCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

391

Amino Acids

44.84

Weight (kDa)

9.79

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 76 - 331 9.5e-69 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 26, 258, 751
AccB1I GGYRCC 1 cut(s) 334
AccBSI CCGCTC 1 cut(s) 262
AccI GTMKAC 1 cut(s) 1011
AccII CGCG 5 cut(s) 46, 174, 176, 245, 247
AciI CCGC 7 cut(s) 32, 219, 260, 443, 607, 652, 968
AclWI GGATC 4 cut(s) 470, 483, 667, 1007
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 3 cut(s) 310, 1025, 1051
AcuI CTGAAG 3 cut(s) 517, 714, 779
AcvI CACGTG 1 cut(s) 52
AcyI GRCGYC 4 cut(s) 23, 255, 335, 748
AdeI CACNNNGTG 1 cut(s) 832
AfaI GTAC 2 cut(s) 116, 527
AfiI CCNNNNNNNGG 2 cut(s) 366, 651
AgsI TTSAA 4 cut(s) 310, 440, 733, 955
AhdI GACNNNNNGTC 1 cut(s) 254
AjiI CACGTC 1 cut(s) 251
AjnI CCWGG 2 cut(s) 337, 588
AjuI GAANNNNNNNTTGG 2 cut(s) 215, 247
Alw26I GTCTC 4 cut(s) 132, 375, 679, 1013
AlwI GGATC 4 cut(s) 470, 483, 667, 1007
AlwNI CAGNNNCTG 1 cut(s) 841
AoxI GGCC 4 cut(s) 214, 385, 604, 969
ApeKI GCWGC 3 cut(s) 133, 1103, 1151
ApoI RAATTY 3 cut(s) 310, 1025, 1051
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AscI GGCGCGCC 1 cut(s) 44
AspLEI GCGC 7 cut(s) 46, 48, 176, 247, 305, 337, 1113
AspS9I GGNCC 4 cut(s) 60, 215, 604, 970
AsuHPI GGTGA 3 cut(s) 16, 618, 1161
AvaII GGWCC 1 cut(s) 60
BaeI ACNNNNGTAYC 2 cut(s) 796, 829
BamHI GGATCC 1 cut(s) 475
BanI GGYRCC 1 cut(s) 334
BbrPI CACGTG 1 cut(s) 52
BbsI GAAGAC 2 cut(s) 26, 76
BbvI GCAGC 3 cut(s) 145, 1090, 1138
BccI CCATC 3 cut(s) 411, 857, 1174
BceAI ACGGC 3 cut(s) 57, 372, 400
BciT130I CCWGG 2 cut(s) 339, 590
BciVI GTATCC 1 cut(s) 824
BclI TGATCA 1 cut(s) 54
BcoDI GTCTC 4 cut(s) 132, 375, 679, 1013
BfoI RGCGCY 2 cut(s) 306, 338
BfuI GTATCC 1 cut(s) 824
BglI GCCNNNNNGGC 1 cut(s) 968
BglII AGATCT 1 cut(s) 179
BisI GCNGC 5 cut(s) 134, 607, 969, 1104, 1152
BlsI GCNGC 5 cut(s) 135, 608, 970, 1105, 1153
Bme1390I CCNGG 2 cut(s) 339, 590
Bme18I GGWCC 1 cut(s) 60
BmeRI GACNNNNNGTC 1 cut(s) 254
BmgBI CACGTC 1 cut(s) 251
BmgT120I GGNCC 4 cut(s) 60, 215, 604, 970
BmiI GGNNCC 4 cut(s) 217, 336, 477, 972
BmrFI CCNGG 2 cut(s) 339, 590
BmsI GCATC 1 cut(s) 949
BpiI GAAGAC 2 cut(s) 26, 76
BplI GAGNNNNNCTC 2 cut(s) 895, 927
BpuEI CTTGAG 2 cut(s) 491, 893
BsaAI YACGTR 1 cut(s) 52
BsaHI GRCGYC 4 cut(s) 23, 255, 335, 748
BsaI GGTCTC 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 1095
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 651
Bse118I RCCGGY 2 cut(s) 212, 296
Bse1I ACTGG 1 cut(s) 725
BseBI CCWGG 2 cut(s) 339, 590
BseDI CCNNGG 1 cut(s) 1095
BseGI GGATG 4 cut(s) 422, 598, 712, 940
BseLI CCNNNNNNNGG 2 cut(s) 366, 651
BseMII CTCAG 2 cut(s) 143, 1109
BseNI ACTGG 1 cut(s) 725
BsePI GCGCGC 1 cut(s) 44
BseRI GAGGAG 1 cut(s) 491
BseX3I CGGCCG 1 cut(s) 385
BseXI GCAGC 3 cut(s) 145, 1090, 1138
BsgI GTGCAG 1 cut(s) 854
Bsh1236I CGCG 5 cut(s) 46, 174, 176, 245, 247
Bsh1285I CGRYCG 1 cut(s) 388
BshFI GGCC 4 cut(s) 216, 387, 606, 971
BshNI GGYRCC 1 cut(s) 334
BsiEI CGRYCG 1 cut(s) 388
BsiSI CCGG 2 cut(s) 213, 297
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 2 cut(s) 366, 651
BsmAI GTCTC 4 cut(s) 132, 375, 679, 1013
BsmFI GGGAC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 4 cut(s) 216, 387, 606, 971
Bso31I GGTCTC 1 cut(s) 375
Bsp1407I TGTACA 2 cut(s) 114, 525
Bsp143I GATC 7 cut(s) 54, 78, 179, 225, 475, 659, 999
BspACI CCGC 7 cut(s) 32, 219, 260, 443, 607, 652, 968
BspANI GGCC 4 cut(s) 216, 387, 606, 971
BspCNI CTCAG 2 cut(s) 142, 1110
BspFNI CGCG 5 cut(s) 46, 174, 176, 245, 247
BspLI GGNNCC 4 cut(s) 217, 336, 477, 972
BspPI GGATC 4 cut(s) 470, 483, 667, 1007
BspT107I GGYRCC 1 cut(s) 334
BspTNI GGTCTC 1 cut(s) 375
BsrBI CCGCTC 1 cut(s) 262
BsrFI RCCGGY 2 cut(s) 212, 296
BsrGI TGTACA 2 cut(s) 114, 525
BsrI ACTGG 1 cut(s) 725
BssAI RCCGGY 2 cut(s) 212, 296
BssECI CCNNGG 1 cut(s) 1095
BssHII GCGCGC 1 cut(s) 44
BssMI GATC 7 cut(s) 54, 78, 179, 225, 475, 659, 999
BssNAI GTATAC 1 cut(s) 1012
BssNI GRCGYC 4 cut(s) 23, 255, 335, 748
BssT1I CCWWGG 1 cut(s) 1095
Bst1107I GTATAC 1 cut(s) 1012
Bst2UI CCWGG 2 cut(s) 339, 590
Bst4CI ACNGT 4 cut(s) 512, 666, 812, 1060
BstACI GRCGYC 4 cut(s) 23, 255, 335, 748
BstAPI GCANNNNNTGC 1 cut(s) 841
BstAUI TGTACA 2 cut(s) 114, 525
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 5 cut(s) 46, 193, 214, 846, 938
BstDEI CTNAG 2 cut(s) 129, 1118
BstF5I GGATG 4 cut(s) 422, 598, 712, 940
BstFNI CGCG 5 cut(s) 46, 174, 176, 245, 247
BstH2I RGCGCY 2 cut(s) 306, 338
BstHHI GCGC 7 cut(s) 46, 48, 176, 247, 305, 337, 1113
BstKTI GATC 7 cut(s) 57, 81, 182, 228, 478, 662, 1002
BstMAI GTCTC 4 cut(s) 132, 375, 679, 1013
BstMBI GATC 7 cut(s) 54, 78, 179, 225, 475, 659, 999
BstMCI CGRYCG 1 cut(s) 388
BstMWI GCNNNNNNNGC 7 cut(s) 213, 218, 227, 302, 841, 968, 1119
BstNI CCWGG 2 cut(s) 339, 590
BstSCI CCNGG 2 cut(s) 337, 588
BstUI CGCG 5 cut(s) 46, 174, 176, 245, 247
BstV1I GCAGC 3 cut(s) 145, 1090, 1138
BstV2I GAAGAC 2 cut(s) 26, 76
BstX2I RGATCY 2 cut(s) 179, 475
BstYI RGATCY 2 cut(s) 179, 475
BstZ17I GTATAC 1 cut(s) 1012
BstZI CGGCCG 1 cut(s) 385
BsuI GTATCC 1 cut(s) 824
BsuRI GGCC 4 cut(s) 216, 387, 606, 971
BtgZI GCGATG 1 cut(s) 863
BtrI CACGTC 1 cut(s) 251
BtsCI GGATG 4 cut(s) 422, 598, 712, 940
BtsI GCAGTG 1 cut(s) 839
BtsIMutI CAGTG 2 cut(s) 671, 839
Cac8I GCNNGC 5 cut(s) 46, 193, 214, 846, 938
CaiI CAGNNNCTG 1 cut(s) 841
CfoI GCGC 7 cut(s) 46, 48, 176, 247, 305, 337, 1113
Cfr10I RCCGGY 2 cut(s) 212, 296
Cfr13I GGNCC 4 cut(s) 60, 215, 604, 970
CseI GACGC 1 cut(s) 190
Csp6I GTAC 2 cut(s) 115, 526
CviAII CATG 5 cut(s) 433, 959, 1007, 1108, 1173
CviQI GTAC 2 cut(s) 115, 526
DdeI CTNAG 2 cut(s) 129, 1118
DinI GGCGCC 1 cut(s) 336
DpnI GATC 7 cut(s) 56, 80, 181, 227, 477, 661, 1001
DpnII GATC 7 cut(s) 54, 78, 179, 225, 475, 659, 999
DraIII CACNNNGTG 1 cut(s) 832
DriI GACNNNNNGTC 1 cut(s) 254
EaeI YGGCCR 1 cut(s) 385
EagI CGGCCG 1 cut(s) 385
Eam1105I GACNNNNNGTC 1 cut(s) 254
EciI GGCGGA 1 cut(s) 667
EclXI CGGCCG 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 1095
Eco31I GGTCTC 1 cut(s) 375
Eco32I GATATC 1 cut(s) 766
Eco47I GGWCC 1 cut(s) 60
Eco52I CGGCCG 1 cut(s) 385
Eco57I CTGAAG 3 cut(s) 517, 714, 779
Eco72I CACGTG 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 1025
EcoRII CCWGG 2 cut(s) 337, 588
EcoRV GATATC 1 cut(s) 766
EcoT14I CCWWGG 1 cut(s) 1095
EgeI GGCGCC 1 cut(s) 336
EheI GGCGCC 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 1095
FaeI CATG 5 cut(s) 436, 962, 1010, 1111, 1176
FalI AAGNNNNNCTT 4 cut(s) 215, 247, 705, 737
FaqI GGGAC 1 cut(s) 242
FatI CATG 5 cut(s) 432, 958, 1006, 1107, 1172
FauI CCCGC 2 cut(s) 226, 267
FbaI TGATCA 1 cut(s) 54
FblI GTMKAC 1 cut(s) 1011
Fnu4HI GCNGC 5 cut(s) 134, 607, 969, 1104, 1152
FokI GGATG 4 cut(s) 429, 605, 719, 927
Fsp4HI GCNGC 5 cut(s) 134, 607, 969, 1104, 1152
GlaI GCGC 7 cut(s) 45, 47, 175, 246, 304, 336, 1112
GluI GCNGC 5 cut(s) 134, 607, 969, 1104, 1152
HaeII RGCGCY 2 cut(s) 306, 338
HaeIII GGCC 4 cut(s) 216, 387, 606, 971
HapII CCGG 2 cut(s) 213, 297
HgaI GACGC 1 cut(s) 190
HhaI GCGC 7 cut(s) 46, 48, 176, 247, 305, 337, 1113
Hin1I GRCGYC 4 cut(s) 23, 255, 335, 748
Hin1II CATG 5 cut(s) 436, 962, 1010, 1111, 1176
Hin6I GCGC 7 cut(s) 44, 46, 174, 245, 303, 335, 1111
HinP1I GCGC 7 cut(s) 44, 46, 174, 245, 303, 335, 1111
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 4 cut(s) 356, 425, 679, 784
HpaII CCGG 2 cut(s) 213, 297
HphI GGTGA 3 cut(s) 16, 618, 1161
Hpy166II GTNNAC 4 cut(s) 360, 580, 755, 1012
Hpy188I TCNGA 7 cut(s) 78, 508, 684, 770, 798, 1031, 1119
Hpy188III TCNNGA 3 cut(s) 183, 289, 470
Hpy8I GTNNAC 4 cut(s) 360, 580, 755, 1012
Hpy99I CGWCG 4 cut(s) 28, 252, 367, 753
HpyAV CCTTC 5 cut(s) 162, 241, 592, 873, 1124
HpyCH4III ACNGT 4 cut(s) 512, 666, 812, 1060
HpyCH4IV ACGT 5 cut(s) 23, 51, 250, 255, 748
HpyCH4V TGCA 4 cut(s) 461, 744, 835, 1154
HpyF10VI GCNNNNNNNGC 7 cut(s) 213, 218, 227, 302, 841, 968, 1119
HpyF3I CTNAG 2 cut(s) 129, 1118
HpySE526I ACGT 5 cut(s) 23, 51, 250, 255, 748
Hsp92I GRCGYC 4 cut(s) 23, 255, 335, 748
Hsp92II CATG 5 cut(s) 436, 962, 1010, 1111, 1176
HspAI GCGC 7 cut(s) 44, 46, 174, 245, 303, 335, 1111
KasI GGCGCC 1 cut(s) 334
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 7 cut(s) 54, 78, 179, 225, 475, 659, 999
LmnI GCTCC 1 cut(s) 903
Lsp1109I GCAGC 3 cut(s) 145, 1090, 1138
LweI GCATC 1 cut(s) 949
MaeII ACGT 5 cut(s) 23, 51, 250, 255, 748
MaeIII GTNAC 4 cut(s) 4, 251, 624, 1136
MalI GATC 7 cut(s) 56, 80, 181, 227, 477, 661, 1001
MbiI CCGCTC 1 cut(s) 262
MboI GATC 7 cut(s) 54, 78, 179, 225, 475, 659, 999
MboII GAAGA 7 cut(s) 28, 31, 76, 147, 150, 153, 277
MflI RGATCY 2 cut(s) 179, 475
MluCI AATT 5 cut(s) 186, 310, 547, 1025, 1051
Mly113I GGCGCC 1 cut(s) 335
MlyI GAGTC 1 cut(s) 365
MnlI CCTC 7 cut(s) 360, 441, 466, 512, 595, 984, 1117
MroNI GCCGGC 1 cut(s) 212
MseI TTAA 3 cut(s) 789, 887, 1160
MslI CAYNNNNRTG 3 cut(s) 410, 963, 1171
MspA1I CMGCKG 2 cut(s) 66, 1151
MspI CCGG 2 cut(s) 213, 297
MspR9I CCNGG 2 cut(s) 339, 590
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 2 cut(s) 339, 590
MvnI CGCG 5 cut(s) 46, 174, 176, 245, 247
MwoI GCNNNNNNNGC 7 cut(s) 213, 218, 227, 302, 841, 968, 1119
NaeI GCCGGC 1 cut(s) 214
NarI GGCGCC 1 cut(s) 335
NdeII GATC 7 cut(s) 54, 78, 179, 225, 475, 659, 999
NgoMIV GCCGGC 1 cut(s) 212
NlaIII CATG 5 cut(s) 436, 962, 1010, 1111, 1176
NlaIV GGNNCC 4 cut(s) 217, 336, 477, 972
NmeAIII GCCGAG 1 cut(s) 311
NmuCI GTSAC 4 cut(s) 4, 251, 624, 1136
PalAI GGCGCGCC 1 cut(s) 44
PauI GCGCGC 1 cut(s) 44
PcsI WCGNNNNNNNCGW 3 cut(s) 20, 87, 261
PctI GAATGC 1 cut(s) 298
PdiI GCCGGC 1 cut(s) 214
PfeI GAWTC 3 cut(s) 425, 679, 784
PflFI GACNNNGTC 2 cut(s) 367, 750
PfoI TCCNGGA 1 cut(s) 588
PkrI GCNGC 5 cut(s) 135, 608, 970, 1105, 1153
PleI GAGTC 1 cut(s) 364
PluTI GGCGCC 1 cut(s) 338
PmaCI CACGTG 1 cut(s) 52
PmlI CACGTG 1 cut(s) 52
PpsI GAGTC 1 cut(s) 364
Ppu21I YACGTR 1 cut(s) 52
Psp6I CCWGG 2 cut(s) 337, 588
PspCI CACGTG 1 cut(s) 52
PspGI CCWGG 2 cut(s) 337, 588
PspN4I GGNNCC 4 cut(s) 217, 336, 477, 972
PspPI GGNCC 4 cut(s) 60, 215, 604, 970
PstNI CAGNNNCTG 1 cut(s) 841
PsuI RGATCY 2 cut(s) 179, 475
PsyI GACNNNGTC 2 cut(s) 367, 750
PteI GCGCGC 1 cut(s) 44
PvuII CAGCTG 2 cut(s) 66, 1151
RsaI GTAC 2 cut(s) 116, 527
RsaNI GTAC 2 cut(s) 115, 526
RseI CAYNNNNRTG 3 cut(s) 410, 963, 1171
SaqAI TTAA 3 cut(s) 789, 887, 1160
SatI GCNGC 5 cut(s) 134, 607, 969, 1104, 1152
Sau3AI GATC 7 cut(s) 54, 78, 179, 225, 475, 659, 999
Sau96I GGNCC 4 cut(s) 60, 215, 604, 970
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 2 cut(s) 339, 590
SfaNI GCATC 1 cut(s) 949
SfoI GGCGCC 1 cut(s) 336
SgsI GGCGCGCC 1 cut(s) 44
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 3 cut(s) 410, 963, 1171
SmlI CTYRAG 2 cut(s) 470, 872
SmoI CTYRAG 2 cut(s) 470, 872
Sse9I AATT 5 cut(s) 186, 310, 547, 1025, 1051
SsiI CCGC 7 cut(s) 32, 219, 260, 443, 607, 652, 968
SspDI GGCGCC 1 cut(s) 334
StyD4I CCNGG 2 cut(s) 337, 588
StyI CCWWGG 1 cut(s) 1095
TaaI ACNGT 4 cut(s) 512, 666, 812, 1060
TaiI ACGT 5 cut(s) 26, 54, 253, 258, 751
TaqI TCGA 6 cut(s) 184, 264, 288, 317, 351, 428
TasI AATT 5 cut(s) 186, 310, 547, 1025, 1051
TatI WGTACW 2 cut(s) 114, 525
TauI GCSGC 2 cut(s) 609, 971
TfiI GAWTC 3 cut(s) 425, 679, 784
Tru1I TTAA 3 cut(s) 789, 887, 1160
Tru9I TTAA 3 cut(s) 789, 887, 1160
TscAI CASTG 2 cut(s) 671, 846
TseFI GTSAC 4 cut(s) 4, 251, 624, 1136
TseI GCWGC 3 cut(s) 133, 1103, 1151
Tsp45I GTSAC 4 cut(s) 4, 251, 624, 1136
TspDTI ATGAA 3 cut(s) 153, 529, 726
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 2 cut(s) 671, 846
Tth111I GACNNNGTC 2 cut(s) 367, 750
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 3 cut(s) 310, 1025, 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 645
XmiI GTMKAC 1 cut(s) 1011
ZraI GACGTC 3 cut(s) 24, 256, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.