Rroxscaffold_2G00091180

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002602
Physical Location & Seq
Reverse (-)
12792611 .. 12795521
2911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00091180.1

Sequence Viewer

Length: 1155 bp
ATGCGCCAACTTTCGAGGTTGAGGTCAAGACTCATCATCATCCTCTGTGTCTCGCTATGTGTACTGGCTTTACTTAGAGTAGTATTCCATTCTGAGTCCAACCTCCAGTCCTCATTCCCCACCTCTCGAAGAAGATCTCGTGCTTTTATTGATATTCAGTTTGTTGGCCCTCCAAAGGTTGCTTTTCTCTTCCTGGTTCGGCGGGATCTCCCTCTTGATTTTCTCTGGGGCACCTTCTTCAAGAATGGTGATAGAGCAAAATTCTCCATTTACGTTCATTCAGAGCCCGGTTTTGTGTTTGATGGATCAACCACAAGGTCTGCATTCTTCTTTGGTCGACAGTTAAACAACAGCATTCAGGTAGTATGGGGAGAATCAAGTATGATCGAAGCAGAGCGGTTACTATTTGAAGCAGCTCTTTATGATCCAGCCAATCAAAGATTTGTCCTTCTCTCAGATAGCTGTGTACCTCTCTACAACTTTAGTTACATTTACAGCTATGTAATGTCATCTCCAAAAAGCTTCGTTGACAGCTTTATTGATGTTAAAGGACATCGTTATGATCCAAAGATGTCACCTACTATACCCAAGGAGAGATGGCGAAAAGGATCCCAGTGGATAACATTGACTCGAAGACATGCTGAAATTGTTGTGGATGACGACACTGTTTTCCCCGTCTTCAGGGAATTCTGCAAGCGATGGCCGCCTACACCCCTGAGTTTTAGAAGGCGACTACTTGCAATCAATTGGACTTTCAATCTTCCATTGTTTACATTTCAGAGGCATCGCAATTGTATCCCGGATGAACATTATGTGCAGACACTACTTGCAATAAGTGGACTTGACAACGAACTTGAACGAAGAGCAGTGACCTATACATTATGGAATCAGTCAACCCTAGAAGGGAAAATAAAATCTTGGCACCCCTTAACATTTGATTATGCAGATGCAGCTCCTCAAAAACTAAGACAAATAAAGGAAATCAAGCACGTCTACAATGAGTATGAACAACACAATGAGTTCTGCCATACCAATTCCAAAGTCACTCCTTGTTTCTTGTTTGCACGAAAGTTCACTCTTGGAGCTGCAATGCGCGTCTTGAGAGAAGGTTTGGTTGGTTCTTATGATGTGACAGCAATACTACGAGATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

384

Amino Acids

44.93

Weight (kDa)

9.54

Isoelectric Point (pI)

55.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 60 - 325 8.9e-67 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 230, 921
AccBSI CCGCTC 1 cut(s) 397
AccI GTMKAC 2 cut(s) 337, 993
AccII CGCG 1 cut(s) 1095
AciI CCGC 3 cut(s) 202, 397, 704
AclWI GGATC 6 cut(s) 213, 313, 419, 557, 603, 616
AcoI YGGCCR 1 cut(s) 701
AcsI RAATTY 2 cut(s) 260, 686
AcuI CTGAAG 1 cut(s) 664
AfaI GTAC 2 cut(s) 63, 468
AfiI CCNNNNNNNGG 3 cut(s) 175, 681, 903
AgsI TTSAA 4 cut(s) 241, 410, 757, 857
AjiI CACGTC 1 cut(s) 991
AjnI CCWGG 1 cut(s) 192
AjuI GAANNNNNNNTTGG 2 cut(s) 1098, 1130
AluBI AGCT 7 cut(s) 416, 462, 498, 522, 534, 953, 1085
AluI AGCT 7 cut(s) 416, 462, 498, 522, 534, 953, 1085
Alw26I GTCTC 1 cut(s) 55
AlwI GGATC 6 cut(s) 213, 313, 419, 557, 603, 616
AoxI GGCC 2 cut(s) 166, 701
ApeKI GCWGC 3 cut(s) 413, 950, 1085
ApoI RAATTY 2 cut(s) 260, 686
AspLEI GCGC 2 cut(s) 6, 1095
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 2 cut(s) 288, 800
AsuHPI GGTGA 2 cut(s) 260, 567
BaeGI GKGCMC 1 cut(s) 233
BamHI GGATCC 1 cut(s) 608
BanI GGYRCC 2 cut(s) 230, 921
BanII GRGCYC 1 cut(s) 288
BauI CACGAG 1 cut(s) 138
BbsI GAAGAC 2 cut(s) 640, 670
BbvI GCAGC 3 cut(s) 425, 962, 1072
BccI CCATC 3 cut(s) 296, 591, 693
BciT130I CCWGG 1 cut(s) 194
BciVI GTATCC 1 cut(s) 806
BcnI CCSGG 2 cut(s) 288, 800
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 2 cut(s) 899, 1153
BfuI GTATCC 1 cut(s) 806
BglII AGATCT 1 cut(s) 134
BisI GCNGC 4 cut(s) 414, 704, 951, 1086
BlsI GCNGC 4 cut(s) 415, 705, 952, 1087
Bme1390I CCNGG 3 cut(s) 194, 288, 800
BmgBI CACGTC 1 cut(s) 991
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 3 cut(s) 232, 610, 923
BmrFI CCNGG 3 cut(s) 194, 288, 800
BmrI ACTGGG 1 cut(s) 607
BmsI GCATC 2 cut(s) 793, 937
BmuI ACTGGG 1 cut(s) 607
BpiI GAAGAC 2 cut(s) 640, 670
BpmI CTGGAG 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 1120
BpuMI CCSGG 2 cut(s) 288, 800
BsaJI CCNNGG 1 cut(s) 588
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 681, 903
Bse1I ACTGG 3 cut(s) 69, 106, 613
Bse3DI GCAATG 1 cut(s) 1095
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 1 cut(s) 588
BseGI GGATG 3 cut(s) 39, 661, 808
BseLI CCNNNNNNNGG 3 cut(s) 175, 681, 903
BseMI GCAATG 1 cut(s) 1095
BseMII CTCAG 3 cut(s) 84, 468, 707
BseNI ACTGG 3 cut(s) 69, 106, 613
BseRI GAGGAG 1 cut(s) 945
BseSI GKGCMC 1 cut(s) 233
BseXI GCAGC 3 cut(s) 425, 962, 1072
BsgI GTGCAG 1 cut(s) 836
Bsh1236I CGCG 1 cut(s) 1095
BshFI GGCC 2 cut(s) 168, 703
BshNI GGYRCC 2 cut(s) 230, 921
BsiSI CCGG 2 cut(s) 288, 800
BslI CCNNNNNNNGG 3 cut(s) 175, 681, 903
BsmAI GTCTC 1 cut(s) 55
BsmI GAATGC 2 cut(s) 323, 354
BsnI GGCC 2 cut(s) 168, 703
Bsp1286I GDGCHC 2 cut(s) 233, 288
Bsp143I GATC 7 cut(s) 134, 205, 305, 384, 424, 562, 608
BspACI CCGC 3 cut(s) 202, 397, 704
BspANI GGCC 2 cut(s) 168, 703
BspCNI CTCAG 3 cut(s) 85, 467, 708
BspFNI CGCG 1 cut(s) 1095
BspLI GGNNCC 3 cut(s) 232, 610, 923
BspPI GGATC 6 cut(s) 213, 313, 419, 557, 603, 616
BspQI GCTCTTC 1 cut(s) 856
BspT107I GGYRCC 2 cut(s) 230, 921
BsrBI CCGCTC 1 cut(s) 397
BsrDI GCAATG 1 cut(s) 1095
BsrI ACTGG 3 cut(s) 69, 106, 613
BssECI CCNNGG 1 cut(s) 588
BssMI GATC 7 cut(s) 134, 205, 305, 384, 424, 562, 608
BssSI CACGAG 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 588
Bst2BI CACGAG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 2 cut(s) 342, 667
Bst6I CTCTTC 2 cut(s) 194, 856
BstC8I GCNNGC 1 cut(s) 695
BstDEI CTNAG 5 cut(s) 74, 93, 454, 716, 965
BstF5I GGATG 3 cut(s) 39, 661, 808
BstFNI CGCG 1 cut(s) 1095
BstHHI GCGC 2 cut(s) 6, 1095
BstKTI GATC 7 cut(s) 137, 208, 308, 387, 427, 565, 611
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 7 cut(s) 134, 205, 305, 384, 424, 562, 608
BstMWI GCNNNNNNNGC 2 cut(s) 703, 950
BstNI CCWGG 1 cut(s) 194
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 3 cut(s) 192, 286, 798
BstSLI GKGCMC 1 cut(s) 233
BstUI CGCG 1 cut(s) 1095
BstV1I GCAGC 3 cut(s) 425, 962, 1072
BstV2I GAAGAC 2 cut(s) 640, 670
BstX2I RGATCY 3 cut(s) 134, 205, 608
BstYI RGATCY 3 cut(s) 134, 205, 608
BsuI GTATCC 1 cut(s) 806
BsuRI GGCC 2 cut(s) 168, 703
BtgZI GCGATG 2 cut(s) 712, 770
BtrI CACGTC 1 cut(s) 991
BtsCI GGATG 3 cut(s) 39, 661, 808
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 3 cut(s) 620, 663, 873
Cac8I GCNNGC 1 cut(s) 695
CfoI GCGC 2 cut(s) 6, 1095
Cfr13I GGNCC 1 cut(s) 167
CseI GACGC 1 cut(s) 1084
Csp6I GTAC 2 cut(s) 62, 467
CviAII CATG 1 cut(s) 638
CviQI GTAC 2 cut(s) 62, 467
DdeI CTNAG 5 cut(s) 74, 93, 454, 716, 965
DpnI GATC 7 cut(s) 136, 207, 307, 386, 426, 564, 610
DpnII GATC 7 cut(s) 134, 205, 305, 384, 424, 562, 608
EaeI YGGCCR 1 cut(s) 701
Eam1104I CTCTTC 2 cut(s) 194, 856
EarI CTCTTC 2 cut(s) 194, 856
Eco130I CCWWGG 1 cut(s) 588
Eco24I GRGCYC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 1150
Eco57I CTGAAG 1 cut(s) 664
EcoRI GAATTC 1 cut(s) 686
EcoRII CCWGG 1 cut(s) 192
EcoRV GATATC 1 cut(s) 1150
EcoT14I CCWWGG 1 cut(s) 588
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 588
FaeI CATG 1 cut(s) 641
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FatI CATG 1 cut(s) 637
FauI CCCGC 1 cut(s) 195
FblI GTMKAC 2 cut(s) 337, 993
Fnu4HI GCNGC 4 cut(s) 414, 704, 951, 1086
FokI GGATG 3 cut(s) 26, 668, 815
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 4 cut(s) 414, 704, 951, 1086
FspBI CTAG 2 cut(s) 899, 1153
GlaI GCGC 2 cut(s) 5, 1094
GluI GCNGC 4 cut(s) 414, 704, 951, 1086
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 2 cut(s) 168, 703
HapII CCGG 2 cut(s) 288, 800
HgaI GACGC 1 cut(s) 1084
HhaI GCGC 2 cut(s) 6, 1095
Hin1II CATG 1 cut(s) 641
Hin6I GCGC 2 cut(s) 4, 1093
HinP1I GCGC 2 cut(s) 4, 1093
HincII GTYRAC 3 cut(s) 338, 529, 894
HindII GTYRAC 3 cut(s) 338, 529, 894
HindIII AAGCTT 1 cut(s) 520
HinfI GANTC 5 cut(s) 30, 95, 374, 628, 886
HpaII CCGG 2 cut(s) 288, 800
HphI GGTGA 2 cut(s) 260, 567
Hpy166II GTNNAC 9 cut(s) 62, 338, 467, 529, 771, 839, 894, 994, 1074
Hpy188I TCNGA 4 cut(s) 94, 283, 457, 780
Hpy188III TCNNGA 5 cut(s) 27, 126, 215, 241, 1099
Hpy8I GTNNAC 9 cut(s) 62, 338, 467, 529, 771, 839, 894, 994, 1074
HpyAV CCTTC 5 cut(s) 244, 458, 720, 896, 1100
HpyCH4III ACNGT 2 cut(s) 342, 667
HpyCH4IV ACGT 2 cut(s) 273, 990
HpyCH4V TGCA 9 cut(s) 323, 693, 740, 817, 830, 944, 950, 1064, 1088
HpyF10VI GCNNNNNNNGC 2 cut(s) 703, 950
HpyF3I CTNAG 5 cut(s) 74, 93, 454, 716, 965
HpySE526I ACGT 2 cut(s) 273, 990
Hsp92II CATG 1 cut(s) 641
HspAI GCGC 2 cut(s) 4, 1093
Kzo9I GATC 7 cut(s) 134, 205, 305, 384, 424, 562, 608
LguI GCTCTTC 1 cut(s) 856
LmnI GCTCC 2 cut(s) 958, 1082
Lsp1109I GCAGC 3 cut(s) 425, 962, 1072
LweI GCATC 2 cut(s) 793, 937
MaeI CTAG 2 cut(s) 899, 1153
MaeII ACGT 2 cut(s) 273, 990
MaeIII GTNAC 6 cut(s) 399, 485, 573, 868, 1042, 1129
MalI GATC 7 cut(s) 136, 207, 307, 386, 426, 564, 610
MbiI CCGCTC 1 cut(s) 397
MboI GATC 7 cut(s) 134, 205, 305, 384, 424, 562, 608
MboII GAAGA 9 cut(s) 141, 144, 181, 229, 319, 645, 670, 752, 873
MfeI CAATTG 2 cut(s) 745, 790
MflI RGATCY 3 cut(s) 134, 205, 608
MhlI GDGCHC 2 cut(s) 233, 288
MluCI AATT 6 cut(s) 260, 645, 686, 745, 790, 1033
MlyI GAGTC 3 cut(s) 24, 104, 622
MmeI TCCRAC 1 cut(s) 123
MseI TTAA 3 cut(s) 344, 546, 929
MslI CAYNNNNRTG 1 cut(s) 558
MspI CCGG 2 cut(s) 288, 800
MspR9I CCNGG 3 cut(s) 194, 288, 800
MunI CAATTG 2 cut(s) 745, 790
Mva1269I GAATGC 2 cut(s) 323, 354
MvaI CCWGG 1 cut(s) 194
MvnI CGCG 1 cut(s) 1095
MwoI GCNNNNNNNGC 2 cut(s) 703, 950
NciI CCSGG 2 cut(s) 288, 800
NdeII GATC 7 cut(s) 134, 205, 305, 384, 424, 562, 608
NlaIII CATG 1 cut(s) 641
NlaIV GGNNCC 3 cut(s) 232, 610, 923
NmuCI GTSAC 4 cut(s) 573, 868, 1042, 1129
NspI RCATGY 1 cut(s) 641
PciSI GCTCTTC 1 cut(s) 856
PctI GAATGC 2 cut(s) 323, 354
PfeI GAWTC 2 cut(s) 374, 886
PfoI TCCNGGA 1 cut(s) 798
PkrI GCNGC 4 cut(s) 415, 705, 952, 1087
PleI GAGTC 3 cut(s) 24, 103, 622
PpsI GAGTC 3 cut(s) 24, 103, 622
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 3 cut(s) 232, 610, 923
PspPI GGNCC 1 cut(s) 167
PsuI RGATCY 3 cut(s) 134, 205, 608
RsaI GTAC 2 cut(s) 63, 468
RsaNI GTAC 2 cut(s) 62, 467
RseI CAYNNNNRTG 1 cut(s) 558
SalI GTCGAC 1 cut(s) 336
SapI GCTCTTC 1 cut(s) 856
SaqAI TTAA 3 cut(s) 344, 546, 929
SatI GCNGC 4 cut(s) 414, 704, 951, 1086
Sau3AI GATC 7 cut(s) 134, 205, 305, 384, 424, 562, 608
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 3 cut(s) 24, 104, 622
ScrFI CCNGG 3 cut(s) 194, 288, 800
SduI GDGCHC 2 cut(s) 233, 288
SfaNI GCATC 2 cut(s) 793, 937
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 1 cut(s) 1099
SmoI CTYRAG 1 cut(s) 1099
Sse9I AATT 6 cut(s) 260, 645, 686, 745, 790, 1033
SsiI CCGC 3 cut(s) 202, 397, 704
SspMI CTAG 2 cut(s) 899, 1153
StyD4I CCNGG 3 cut(s) 192, 286, 798
StyI CCWWGG 1 cut(s) 588
TaaI ACNGT 2 cut(s) 342, 667
TaiI ACGT 2 cut(s) 276, 993
TaqI TCGA 5 cut(s) 14, 127, 337, 387, 631
TasI AATT 6 cut(s) 260, 645, 686, 745, 790, 1033
TatI WGTACW 1 cut(s) 61
TauI GCSGC 1 cut(s) 706
TfiI GAWTC 2 cut(s) 374, 886
Tru1I TTAA 3 cut(s) 344, 546, 929
Tru9I TTAA 3 cut(s) 344, 546, 929
TscAI CASTG 3 cut(s) 620, 670, 873
TseFI GTSAC 4 cut(s) 573, 868, 1042, 1129
TseI GCWGC 3 cut(s) 413, 950, 1085
Tsp45I GTSAC 4 cut(s) 573, 868, 1042, 1129
TspDTI ATGAA 3 cut(s) 266, 819, 1020
TspRI CASTG 3 cut(s) 620, 670, 873
XapI RAATTY 2 cut(s) 260, 686
XceI RCATGY 1 cut(s) 641
XmiI GTMKAC 2 cut(s) 337, 993
XspI CTAG 2 cut(s) 899, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.